EY744376

Overview
NameEY744376
Unique NameEY744376
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length977
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBC22_ARATH (Ubiquitin carrier protein E2 22 OS=Arabidopsis thaliana GN=UBC22 PE=1 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 4.324e-59
Identity = 103/119 (86.55%), Postives = 111/119 (93.28%), Query Frame = 3 
Query:  159 MATNENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            MA+NENLPPNVIKQLAKELKSLDESPP+GIKV VND+DFS I ADIEGP GTPYENG+FRMKL LSHDFPHSPPKGYF+TKIFHPN+A+NGEICVNTLKKDWNPSLGLRHVL VVR  +
Sbjct:    1 MASNENLPPNVIKQLAKELKSLDESPPDGIKVVVNDEDFSQICADIEGPVGTPYENGLFRMKLALSHDFPHSPPKGYFMTKIFHPNVASNGEICVNTLKKDWNPSLGLRHVLSVVRCLL 119

HSP 2 Score: 28.4906 bits (62), Expect = 4.324e-59
Identity = 16/31 (51.61%), Postives = 20/31 (64.52%), Query Frame = 2 
Query:  527 FRESALNEQAGKNAA*KLTKQYPKTARIYIG 619
            F ESALNEQAGK    +   +Y + AR+Y G
Sbjct:  123 FPESALNEQAGKMLL-ENYDEYARHARLYTG 152

HSP 3 Score: 22.3274 bits (46), Expect = 4.324e-59
Identity = 8/8 (100.00%), Postives = 8/8 (100.00%), Query Frame = 1 
Query:  508 CLLIEPFP 531
            CLLIEPFP
Sbjct:  117 CLLIEPFP 124
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_XENTR (Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropicalis GN=ube2s PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 2.518e-39
Identity = 69/115 (60.00%), Postives = 91/115 (79.13%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENLPP++I+Q+ KE+ +L   PPEGIK+  N++D + +  +IEGP GTPY  G+FRMKL+L  DFP +PPKGYFLTKIFHPN++TNGEICVN LKKDW   LG+RHVL+ ++  +
Sbjct:    6 ENLPPHIIRQVYKEVSTLTSDPPEGIKIIPNEEDITDVQVNIEGPEGTPYAGGIFRMKLILGKDFPAAPPKGYFLTKIFHPNVSTNGEICVNVLKKDWKAELGIRHVLLTIKCLL 120
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROVI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila virilis GN=GJ15767 PE=3 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.038e-39
Identity = 71/115 (61.74%), Postives = 92/115 (80.00%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ KEL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  GVFR+KL LS DFP +PPK YFLTKIFHPN+A+NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMKELQDMETTPPEGIKVLINESDVTDIQAFIDGPAGTPYAVGVFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 3.038e-39
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROGR (Ubiquitin-conjugating enzyme E2 S OS=Drosophila grimshawi GN=GH12079 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 3.958e-39
Identity = 70/115 (60.87%), Postives = 92/115 (80.00%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ KEL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  G+FR+KL LS DFP +PPK YFLTKIFHPN+A+NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMKELQDMESTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVASNGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 3.958e-39
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_NEMVE (Ubiquitin-conjugating enzyme E2 S OS=Nematostella vectensis GN=v1g237158 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 3.959e-39
Identity = 71/115 (61.74%), Postives = 89/115 (77.39%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            EN  P +IKQ+A+E+  L   PPEGIKV  ND+D + I A IEGP GTPYE G+F++KL+L  DFP +PPKG+FLTKIFHPN+A NGEICVNTLKKDW P LG++ VL+ V+  +
Sbjct:    9 ENFSPQIIKQVAREIHGLTNDPPEGIKVFSNDEDITDIQASIEGPTGTPYEGGIFKIKLVLGKDFPAAPPKGFFLTKIFHPNVAKNGEICVNTLKKDWKPDLGIKQVLLTVKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 3.959e-39
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROMO (Ubiquitin-conjugating enzyme E2 S OS=Drosophila mojavensis GN=GI11085 PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 6.721e-39
Identity = 70/115 (60.87%), Postives = 91/115 (79.13%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ KEL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  G+FR+KL LS DFP +PPK YFLTKIFHPN+A NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMKELQDMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 6.721e-39
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROWI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila willistoni GN=GK16201 PE=3 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 8.754e-39
Identity = 70/115 (60.87%), Postives = 91/115 (79.13%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ +EL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  GVFR+KL LS DFP +PPK YFLTKIFHPN+A NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMRELQEMENTPPEGIKVLINESDVTDIQALIDGPAGTPYAIGVFRVKLTLSKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 8.754e-39
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_RANCA (Ubiquitin-conjugating enzyme E2 S OS=Rana catesbeiana GN=ube2s PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 9.567e-39
Identity = 69/115 (60.00%), Postives = 90/115 (78.26%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENLPP+VI+Q+ KE+ +L   PPEGIK+  N++D + +   IEGP GTPY  G+FRMKL+L  DFP +PPKGYFLTKIFHPN+++NGEICVN LKKDW   LG+RHVL+ ++  +
Sbjct:    6 ENLPPHVIRQVYKEVSTLTPDPPEGIKIIPNEEDITDVQVSIEGPEGTPYAGGIFRMKLILGKDFPAAPPKGYFLTKIFHPNVSSNGEICVNVLKKDWKAELGIRHVLLTIKCLL 120
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROYA (Ubiquitin-conjugating enzyme E2 S OS=Drosophila yakuba GN=GE15576 PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.936e-38
Identity = 69/115 (60.00%), Postives = 91/115 (79.13%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ +EL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  GVFR+KL L+ DFP +PPK YFLTKIFHPN+A NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 1.936e-38
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
[Close]
BLAST of EY744376 vs. ExPASy Swiss-Prot
Match: UBE2S_DROER (Ubiquitin-conjugating enzyme E2 S OS=Drosophila erecta GN=GG18165 PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 1.936e-38
Identity = 69/115 (60.00%), Postives = 91/115 (79.13%), Query Frame = 3 
Query:  171 ENLPPNVIKQLAKELKSLDESPPEGIKVGVNDDDFSTIFADIEGPAGTPYENGVFRMKLLLSHDFPHSPPKGYFLTKIFHPNIATNGEICVNTLKKDWNPSLGLRHVLIVVRVFI 515
            ENL P  I+Q+ +EL+ ++ +PPEGIKV +N+ D + I A I+GPAGTPY  GVFR+KL L+ DFP +PPK YFLTKIFHPN+A NGEICVNTLKKDW P LG++H+L+ ++  +
Sbjct:    9 ENLSPQTIRQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGVFRVKLTLNKDFPQTPPKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLL 123

HSP 2 Score: 21.9422 bits (45), Expect = 1.936e-38
Identity = 9/10 (90.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  533 ESALNEQAGK 562
            ESALNE+AGK
Sbjct:  129 ESALNEEAGK 138
The following BLAST results are available for this feature:
BLAST of EY744376 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 167
Match NameE-valueIdentityDescription
UBC22_ARATH4.324e-5986.55Ubiquitin carrier protein E2 22 OS=Arabidopsis tha... [more]
UBE2S_XENTR2.518e-3960.00Ubiquitin-conjugating enzyme E2 S OS=Xenopus tropi... [more]
UBE2S_DROVI3.038e-3961.74Ubiquitin-conjugating enzyme E2 S OS=Drosophila vi... [more]
UBE2S_DROGR3.958e-3960.87Ubiquitin-conjugating enzyme E2 S OS=Drosophila gr... [more]
UBE2S_NEMVE3.959e-3961.74Ubiquitin-conjugating enzyme E2 S OS=Nematostella ... [more]
UBE2S_DROMO6.721e-3960.87Ubiquitin-conjugating enzyme E2 S OS=Drosophila mo... [more]
UBE2S_DROWI8.754e-3960.87Ubiquitin-conjugating enzyme E2 S OS=Drosophila wi... [more]
UBE2S_RANCA9.567e-3960.00Ubiquitin-conjugating enzyme E2 S OS=Rana catesbei... [more]
UBE2S_DROYA1.936e-3860.00Ubiquitin-conjugating enzyme E2 S OS=Drosophila ya... [more]
UBE2S_DROER1.936e-3860.00Ubiquitin-conjugating enzyme E2 S OS=Drosophila er... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-007-H10-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744376 ID=EY744376; Name=EY744376; organism=Citrus sinensis; type=EST; length=977bp
ATCAGACAAGGTGCTTTCGGCATTCTGCTTACAAATTTTTTATCACTAAG
CCTTTAAGTTCAAAAAGCAACGGAACATTCTTTGCCAAGGTTCTAATTTC
CTGCCAGTGACAGCGCAAAAGAAGAGCTATTACAAGGATCCTGTCATCTT
TTGTTGACATGGCTACTAATGAAAATCTTCCACCAAATGTAATAAAGCAA
CTTGCTAAGGAATTGAAGAGTCTTGATGAATCTCCTCCTGAGGGCATTAA
AGTAGGCGTAAATGATGATGATTTTTCAACCATATTTGCTGATATTGAAG
GCCCAGCTGGAACTCCTTATGAGAATGGTGTTTTCCGCATGAAGTTGTTA
TTATCTCATGACTTCCCGCACTCCCCTCCAAAAGGTTACTTCCTGACGAA
GATATTCCATCCAAATATTGCAACAAATGGTGAAATTTGTGTGAATACAC
TAAAAAAAGATTGGAATCCAAGTCTTGGATTGAGACATGTTCTCATCGTA
GTCCGGGTGTTTATTGATTGAACCATTTCCGGGAATCAGCATTGAATGAA
CAGGCTGGTAAGAACGCTGCCTGAAAATTAACGAAGCAATACCCCAAAAC
TGCCAGGATTTACATTGGGAATTCATGCTAAAGCAAATCCACAAATATCC
GTCACGGACCTATTTTCCAACCTCCCACGGCCGCTGATCGTAGACCCAAA
CATATACCTCATATGGCCCCTAAGAAAGAAACGCCCAATCTGGCGGACCC
ACTCCATCTGCCCCTTCGACGCACCCCACAAACTCCTTCCCCAACCCCCC
CCTCGAAGGGAGAGACAATATCTCGCAAATCGCCCAACCACCTACTTTCC
CCCACCTACAGACACACGGGACCCTCGCCCGCGTATCATACGAACAATAC
CCCCTTAACCCACCACCACCCATCACACCACACAGTACCAGACCCTGACC
CCGTAATGCACCGTACCCCAACTTGCT
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