EY660033

Overview
NameEY660033
Unique NameEY660033
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length905
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7523 contig Csv1_Contig7523:1..908. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: ACS2_RUTGR (Acridone synthase 2 OS=Ruta graveolens GN=ACS2 PE=1 SV=1)

HSP 1 Score: 308.531 bits (789), Expect = 3.314e-83
Identity = 164/269 (60.97%), Postives = 192/269 (71.38%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSEKQSPLSVGRLIPIL--IL*WVRXXXXXXXXXXXXXXXXNIH*ASLYHIVSGSQTLLPDSDGXMKDH-T*GGSKVHLK 903
            M +++E+R AQ++EG A ILAI TATP N  IQADYPDYYF IT SEHMT            SMIRKRHMC ++E LKANP +C HMG SL+ARQDI++VE P++GKEAA KAIKEWG PKS+ITHLIFCTSAGVDMPGADYQLTR++GLNP VKRMMIYQQGCYAG T+LRLAKDLAENNKGSRVLVVCSE  +P   G     +  ++                    ++   +LY+IVS SQ LLPDSDG ++ H    G  VHLK
Sbjct:    1 MESLKEMRKAQKSEGPAAILAIGTATPDNVYIQADYPDYYFKITKSEHMTELKDKFKTLCEKSMIRKRHMCFSQEFLKANPEVCKHMGKSLNARQDIAVVETPRIGKEAAVKAIKEWGHPKSSITHLIFCTSAGVDMPGADYQLTRMLGLNPSVKRMMIYQQGCYAGGTVLRLAKDLAENNKGSRVLVVCSELTAPTFRGPSPDAVDSLVGQALFADGAAALVVGADPDTSVE-RALYYIVSASQMLLPDSDGAIEGHIREEGLTVHLK 268
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHS3_SOYBN (Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV=1)

HSP 1 Score: 294.278 bits (752), Expect = 1.284e-82
Identity = 143/192 (74.48%), Postives = 163/192 (84.90%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++EEIRNAQRAEG AT++AI TATPPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M L EEILK NP++C +M  SLDARQD+ +VEVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVEEIRNAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 32.7278 bits (73), Expect = 1.284e-82
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGP+ TH+DSLVGQ
Sbjct:  194 TAVTFRGPTDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHSY_PUELO (Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 6.324e-82
Identity = 144/192 (75.00%), Postives = 160/192 (83.33%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++ EIR AQRAEG ATILAI TA PPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M LTEEILK NPNMC +M  SLDARQD+ +VEVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+ +GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVAEIRQAQRAEGPATILAIGTANPPNCVDQSTYPDYYFRITNSEHMTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKQLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 33.8834 bits (76), Expect = 6.324e-82
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGPS TH+DSLVGQ
Sbjct:  194 TAVTFRGPSDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHS1_SOYBN (Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 6.325e-82
Identity = 142/192 (73.96%), Postives = 162/192 (84.38%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++EEIR AQRAEG AT++AI TATPPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M L EEILK NP++C +M  SLDARQD+ +VEVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 32.7278 bits (73), Expect = 6.325e-82
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGP+ TH+DSLVGQ
Sbjct:  194 TAVTFRGPTDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHSB_PEA (Chalcone synthase 1B OS=Pisum sativum GN=CHS-1B PE=3 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.076e-81
Identity = 142/192 (73.96%), Postives = 162/192 (84.38%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MVT++EIR AQ+AEG AT+LAI TATPPNCV Q+ YPDYYF ITNSEH T            SMI+KR+M LTEEILK NP++C +M  SLDARQD+ +VEVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVTVDEIRQAQKAEGPATVLAIGTATPPNCVDQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMIKKRYMHLTEEILKENPSVCEYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPHVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 33.8834 bits (76), Expect = 1.076e-81
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGPS TH+DSLVGQ
Sbjct:  194 TAVTFRGPSDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHSY_VIGUN (Chalcone synthase OS=Vigna unguiculata GN=CHS PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.076e-81
Identity = 143/192 (74.48%), Postives = 161/192 (83.85%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++ EIR AQRAEG ATILAI TATPPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M +TEEILK NP+MC +M  SLDARQDI +VEVP+LGKEAA KAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVSEIRQAQRAEGPATILAIGTATPPNCVDQSTYPDYYFRITNSEHMTDLKEKFQRMCDKSMIKKRYMHVTEEILKENPSMCAYMAPSLDARQDIVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 33.8834 bits (76), Expect = 1.076e-81
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGPS TH+DSLVGQ
Sbjct:  194 TAVTFRGPSDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: ACS3_RUTGR (Probable acridone synthase 3 OS=Ruta graveolens GN=ACS3 PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 1.392e-81
Identity = 162/269 (60.22%), Postives = 190/269 (70.63%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSEKQSPLSVGRLIPIL--IL*WVRXXXXXXXXXXXXXXXXNIH*ASLYHIVSGSQTLLPDSDGXMKDH-T*GGSKVHLK 903
            M +++E+R AQ +EG A ILAI TA P N  +QADYPDYYF +T SEHMT            SMIRKRHMC +EE LKANP +C HMG SL+ARQDI++VE P+LG EAA KAIKEWGQPKS+ITHLIFC+SAGVDMPGADYQLTR++GLNP VKRMMIYQQGCYAG T+LRLAKDLAENNKGSRVLVVCSE  +P   G     +  ++                    +I   +LY++VS SQ LLPDSDG ++ H    G  VHLK
Sbjct:    1 MESLKEMRKAQMSEGPAAILAIGTANPDNVYMQADYPDYYFKMTKSEHMTELKDKFRTLCEKSMIRKRHMCFSEEFLKANPEVCKHMGKSLNARQDIAVVETPRLGNEAAVKAIKEWGQPKSSITHLIFCSSAGVDMPGADYQLTRILGLNPSVKRMMIYQQGCYAGGTVLRLAKDLAENNKGSRVLVVCSELTAPTFRGPSPDAVDSLVGQALFADGAAALVVGADPDSSIE-RALYYLVSASQMLLPDSDGAIEGHIREEGLTVHLK 268
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHS5_SOYBN (Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 1.404e-81
Identity = 141/192 (73.44%), Postives = 162/192 (84.38%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++EEIR AQRAEG AT++AI TATPPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M L EEILK NP++C +M  SLDARQD+ ++EVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVEEIRQAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 32.7278 bits (73), Expect = 1.404e-81
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGP+ TH+DSLVGQ
Sbjct:  194 TAVTFRGPTDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHS2_SOYBN (Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 1.404e-81
Identity = 141/192 (73.44%), Postives = 162/192 (84.38%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++EEIR AQRAEG AT++AI TATPPNCV Q+ YPDYYF ITNSEHMT            SMI+KR+M L EEILK NP++C +M  SLDARQD+ ++EVPKLGKEAATKAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 32.7278 bits (73), Expect = 1.404e-81
Identity = 14/19 (73.68%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGP+ TH+DSLVGQ
Sbjct:  194 TAVTFRGPTDTHLDSLVGQ 212
[Close]
BLAST of EY660033 vs. ExPASy Swiss-Prot
Match: CHSY_PHAVU (Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS17 PE=2 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.831e-81
Identity = 144/192 (75.00%), Postives = 159/192 (82.81%), Query Frame = 1 
Query:  106 MVTMEEIRNAQRAEGLATILAISTATPPNCVIQADYPDYYFGITNSEHMTXXXXXXXXXXXXSMIRKRHMCLTEEILKANPNMCLHMGTSLDARQDISLVEVPKLGKEAATKAIKEWGQPKSNITHLIFCTSAGVDMPGADYQLTRLIGLNPDVKRMMIYQQGCYAGATILRLAKDLAENNKGSRVLVVCSE 681
            MV++ EIR AQRAEG ATILAI TATP NCV Q+ YPDYYF ITNSEHMT            SMI+KR+M L EEILK NPNMC +M  SLDARQDI +VEVPKLGKEAA KAIKEWGQPKS ITHLIFCT++GVDMPGADYQLT+L+GL P VKR M+YQQGC+AG T+LRLAKDLAENNKG+RVLVVCSE
Sbjct:    1 MVSVSEIRQAQRAEGPATILAIGTATPSNCVDQSTYPDYYFRITNSEHMTDLKEKFQRMCDKSMIKKRYMHLNEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 33.8834 bits (76), Expect = 1.831e-81
Identity = 15/19 (78.95%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:  684 TIPTFRGPSYTHIDSLVGQ 740
            T  TFRGPS TH+DSLVGQ
Sbjct:  194 TAVTFRGPSDTHLDSLVGQ 212
The following BLAST results are available for this feature:
BLAST of EY660033 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 145
Match NameE-valueIdentityDescription
ACS2_RUTGR3.314e-8360.97Acridone synthase 2 OS=Ruta graveolens GN=ACS2 PE=... [more]
CHS3_SOYBN1.284e-8274.48Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV... [more]
CHSY_PUELO6.324e-8275.00Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 S... [more]
CHS1_SOYBN6.325e-8273.96Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV... [more]
CHSB_PEA1.076e-8173.96Chalcone synthase 1B OS=Pisum sativum GN=CHS-1B PE... [more]
CHSY_VIGUN1.076e-8174.48Chalcone synthase OS=Vigna unguiculata GN=CHS PE=2... [more]
ACS3_RUTGR1.392e-8160.22Probable acridone synthase 3 OS=Ruta graveolens GN... [more]
CHS5_SOYBN1.404e-8173.44Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV... [more]
CHS2_SOYBN1.404e-8173.44Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV... [more]
CHSY_PHAVU1.831e-8175.00Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS1... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-017-G10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY660033 ID=EY660033; Name=EY660033; organism=Citrus sinensis; type=EST; length=905bp
TGCAGGACCGGTCCGGAATTCTTCAACACCGCGGGCGTNCGGAAAGCGCT
TCCTNAAAATAAACTAAGTTTAAGTACTGTTTTGGAAGCAATCTGACGAT
CAGAGATGGTCACCATGGAGGAGATTAGAAACGCTCAGCGAGCCGAGGGC
CTGGCGACCATCCTCGCCATCAGCACGGCAACGCCGCCCAATTGTGTCAT
CCAAGCTGATTATCCTGACTATTACTTCGGGATCACCAACAGCGAGCACA
TGACTGAGCTCAAAGAGAAGTTCAAGCTCTTGTGTGAGAAGTCGATGATA
AGAAAGCGTCACATGTGCTTAACAGAAGAGATTTTAAAAGCAAACCCTAA
TATGTGCTTACACATGGGGACATCACTCGATGCGCGCCAGGACATTTCTC
TTGTTGAAGTACCAAAGCTTGGCAAAGAGGCGGCAACCAAAGCTATCAAA
GAATGGGGACAGCCCAAATCCAATATCACCCATCTCATTTTCTGCACATC
TGCAGGCGTCGACATGCCTGGCGCTGACTATCAGCTCACAAGACTCATCG
GCCTCAACCCGGATGTTAAGCGTATGATGATATATCAACAAGGGTGTTAT
GCTGGCGCCACCATCCTTCGTCTTGCTAAAGATTTGGCTGAAAACAACAA
GGGCTCTCGTGTTCTTGTTGTTTGTTCTGAGAAACAATCCCCACTTTCCG
TGGGCCGTCTTATACCCATATTGATTCTTTAGTGGGTCAGGCTCTTTTCG
CTGATGGTGCTGCTGCACTGATTGNTGGTGCTGATCCTGAACATCCATTG
AGCGTCATTGTATCATATTGTGTCGGGCTCACAGACGCTTCTGCCTGATT
CTGATGGTNGCATGAAGGACCATACGTGAGGGGGGTCTAAGGTTCATTTG
AAGAA
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