EY659950

Overview
NameEY659950
Unique NameEY659950
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length847
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9274 contig Csv1_Contig9274:493..1346. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA1_LARTR (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata GN=RCA1 PE=2 SV=1)

HSP 1 Score: 445.277 bits (1144), Expect = 2.035e-124
Identity = 217/234 (92.74%), Postives = 222/234 (94.87%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHIT N +SLPNIK+PLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFR DNVADDDIVKLVDTFPGQSIDFFGAL  RVY D VR W+S + V
Sbjct:  133 IAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARVYHDEVRKWVSEVGV 366
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA2_LARTR (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata GN=RCA2 PE=2 SV=1)

HSP 1 Score: 439.499 bits (1129), Expect = 1.117e-122
Identity = 213/234 (91.03%), Postives = 221/234 (94.44%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHIT N +SLPNIK+PLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFR DNV ++DIVK+VD FPGQSIDFFGAL  RVYDD VR W+S + V
Sbjct:  133 IAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVVDQFPGQSIDFFGALRARVYDDEVRKWVSEVGV 366
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA_ORYSJ (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica GN=RCA PE=1 SV=2)

HSP 1 Score: 437.187 bits (1123), Expect = 5.543e-122
Identity = 211/234 (90.17%), Postives = 221/234 (94.44%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHI+ N M+LPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKE+NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTR+DR+GVCKGIFR DNV D+DIVK+VD+FPGQSIDFFGAL  RVYDD VR W+S   V
Sbjct:  124 IAPAFMDKLVVHISKNFMTLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCKGIFRTDNVPDEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDTGV 357
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA_MALDO (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica GN=RCA PE=2 SV=1)

HSP 1 Score: 434.106 bits (1115), Expect = 4.692e-121
Identity = 213/234 (91.03%), Postives = 221/234 (94.44%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHIT N M+LPN+KVPLILGIWGGKGQGKSFQCELVFAKM I+PIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMC L INDLDAGAGR+GGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVC GIFR+DNVA +DIVKLVDTFPGQSIDFFGAL  RVYDD VR WI+G+ V
Sbjct:  133 IAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSFQCELVFAKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWITGVGV 366
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA_PHAAU (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus aureus GN=RCA PE=2 SV=2)

HSP 1 Score: 430.639 bits (1106), Expect = 5.188e-120
Identity = 209/234 (89.32%), Postives = 218/234 (93.16%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAF+DKLVVHIT N M+LPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+I KGKMC L INDLDAGAGR+GGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKEEN RVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTR+DR+GVCKGIFR D V ++DI KLVDTFPGQSIDFFGAL  RVYDD VR WISG+ V
Sbjct:  135 IAPAFLDKLVVHITKNFMTLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIAKGKMCALFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGVCKGIFRTDGVPEEDITKLVDTFPGQSIDFFGALRARVYDDEVRKWISGVGV 368
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA_SOLPN (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Solanum pennellii PE=2 SV=1)

HSP 1 Score: 428.713 bits (1101), Expect = 1.971e-119
Identity = 206/234 (88.03%), Postives = 218/234 (93.16%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHIT N ++LPNIKVPLILG+WGGKGQGKSFQCELVF KMGINPIMMSAGELESGNAGEPAKLIRQRYREAA+IIRKG MCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNK+EN RVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFR DNV ++ +VK+VD+FPGQSIDFFGAL  RVYDD VR W+SG  +
Sbjct:  132 IAPAFMDKLVVHITKNFLTLPNIKVPLILGVWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRKGNMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSGTGI 365
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA_CUCSA (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 428.328 bits (1100), Expect = 2.575e-119
Identity = 207/234 (88.46%), Postives = 216/234 (92.31%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKL VHI  N ++LPNIKVPLILG+WGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKMCCL INDLDAGAGR+GGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRM+KFYWAPTREDRIG+C GIFR D V  +DIVKLVDTFPGQSIDFFGAL  RVYDD VR W  G+ V
Sbjct:  130 IAPAFMDKLTVHIVKNFLTLPNIKVPLILGVWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMDKFYWAPTREDRIGICTGIFRTDGVPFEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWAVGVGV 363
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCAA_HORVU (Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic OS=Hordeum vulgare GN=RCAA PE=2 SV=1)

HSP 1 Score: 427.557 bits (1098), Expect = 4.392e-119
Identity = 203/234 (86.75%), Postives = 218/234 (93.16%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVH++ N M+LPNIK+PLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+I+KGKMCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIAD PT VQLPGMYNK ENPRVPI+VTGNDFSTLYAPLIRDGRMEKFYWAPTR+DRIGVCKGIF+ DNV+D+ +VK+VDTFPGQSIDFFGAL  RVYDD VR W+    +
Sbjct:  123 IAPAFMDKLVVHLSKNFMTLPNIKIPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADMIKKGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADAPTNVQLPGMYNKRENPRVPIVVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRIGVCKGIFQTDNVSDESVVKIVDTFPGQSIDFFGALRARVYDDEVRKWVGSTGI 356
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA1_TOBAC (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 427.557 bits (1098), Expect = 4.392e-119
Identity = 207/234 (88.46%), Postives = 215/234 (91.88%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAPAFMDKLVVHIT N + LPNIKVPLILGIWGGKGQGKSFQCELVF KMGINPIMMSAGELESGNAGEPAKLIRQRYREAA+IIRKG MCCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNK+EN RVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVC GIFR DNV  +D+VK+VD FPGQSIDFFGAL  RVYDD VR W+SG  +
Sbjct:  137 IAPAFMDKLVVHITKNFLKLPNIKVPLILGIWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRKGNMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRTDNVPAEDVVKIVDNFPGQSIDFFGALRARVYDDEVRKWVSGTGI 370
[Close]
BLAST of EY659950 vs. ExPASy Swiss-Prot
Match: RCA2_TOBAC (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Nicotiana tabacum GN=RCA PE=2 SV=1)

HSP 1 Score: 426.787 bits (1096), Expect = 7.492e-119
Identity = 204/234 (87.18%), Postives = 217/234 (92.74%), Query Frame = 2 
Query:   38 IAPAFMDKLVVHITNNPMSLPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCCLMINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTCVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRNDNVADDDIVKLVDTFPGQSIDFFGALXXRVYDDXVRNWISGIXV 739
            IAP+FMDKLVVHIT N + LPNIKVPLILG+WGGKGQGKSFQCELVF KMGINPIMMSAGELESGNAGEPAKLIRQRYREAA+IIRKG +CCL INDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPT VQLPGMYNK+EN RVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFR DNV ++ ++K+VDTFPGQSIDFFGAL  RVYDD VR W+SG  +
Sbjct:  137 IAPSFMDKLVVHITKNFLKLPNIKVPLILGVWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIRKGNICCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQENARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVIKIVDTFPGQSIDFFGALRARVYDDEVRKWVSGTGI 370
The following BLAST results are available for this feature:
BLAST of EY659950 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 20
Match NameE-valueIdentityDescription
RCA1_LARTR2.035e-12492.74Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA2_LARTR1.117e-12291.03Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_ORYSJ5.543e-12290.17Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_MALDO4.692e-12191.03Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_PHAAU5.188e-12089.32Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_SOLPN1.971e-11988.03Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA_CUCSA2.575e-11988.46Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCAA_HORVU4.392e-11986.75Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA1_TOBAC4.392e-11988.46Ribulose bisphosphate carboxylase/oxygenase activa... [more]
RCA2_TOBAC7.492e-11987.18Ribulose bisphosphate carboxylase/oxygenase activa... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-016-H05-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY659950 ID=EY659950; Name=EY659950; organism=Citrus sinensis; type=EST; length=847bp
TCAAAAAAGGGACGCGTNCGACGATCGTTGCTNAAAAATTGCCCCTGCTT
TCATGGACAAGCTTGTTGTTCACATCACCAATAACCCCATGTCCCTGCCC
AACATCAAGGTTCCTCTCATCTTGGGTATTTGGGGAGGCAAAGGACAAGG
AAAATCATTCCAATGTGAGCTTGTTTTCGCCAAGATGGGCATCAACCCAA
TTATGATGAGTGCCGGAGAATTGGAAAGTGGCAATGCTGGAGAACCCGCT
AAGTTGATCAGGCAGAGGTACCGTGAGGCAGCTGACATTATCAGGAAGGG
AAAAATGTGCTGCCTCATGATCAATGATCTTGATGCAGGAGCTGGTCGTA
TGGGTGGCACCACCCAGTACACAGTTAACAACCAAATGGTTAACGCCACA
CTCATGAACATTGCTGATAACCCAACTTGTGTCCAACTCCCTGGTATGTA
CAACAAGGAGGAGAACCCCCGTGTCCCAATCATAGTCACCGGTAATGACT
TCTCAACATTGTATGCTCCTCTCATCCGTGATGGTCGTATGGAGAAATTC
TACTGGGCACCTACAAGGGAGGACCGTATTGGTGTGTGCAAGGGTATCTT
CAGGAATGACAATGTGGCTGATGACGACATTGTCAAGCTTGTTGACACCT
TCCCCGGCCAATCCATTGATTTCTTTGGAGCCCTGANGGNCAGAGTCTAT
GATGATGANGTGAGGAATTGGATCTCTGGTATTGNTGTNGAAGCATTGNC
AAGAGCCTTGTGACTCAAAGAAGCAGCCCCACATTCGAGCACCAAGGATG
ACATGGNAGAGCTGCTCGAGTCGGAAACATGATTGTGCAAGAGCAGA
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