EY674945

Overview
NameEY674945
Unique NameEY674945
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length606
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEPF (Imidazolonepropionase OS=Pseudomonas fluorescens (strain Pf0-1) GN=hutI PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.884e-21
Identity = 30/39 (76.92%), Postives = 34/39 (87.18%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            ++LP  +Y   +DLQGAWVTPGLIDCHTHTVFGGNRSGE
Sbjct:   40 SQLPSGEYPAVNDLQGAWVTPGLIDCHTHTVFGGNRSGE 78

HSP 2 Score: 51.2174 bits (121), Expect = 1.884e-21
Identity = 26/44 (59.09%), Postives = 30/44 (68.18%), Query Frame = -2 
Query:  102 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAEG 233
            K    +  +ATMAQG   IIE+AAIVTSG LIEWIGPR +   G
Sbjct:    2 KTLWQHCHVATMAQGVYSIIEDAAIVTSGALIEWIGPRSQLPSG 45
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSESM (Imidazolonepropionase OS=Pseudomonas syringae pv. tomato GN=hutI PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 6.120e-19
Identity = 29/39 (74.36%), Postives = 32/39 (82.05%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AEL + ++    DL GAWVTPGLIDCHTHTVFGGNRSGE
Sbjct:   40 AELAEPEHDNCIDLGGAWVTPGLIDCHTHTVFGGNRSGE 78

HSP 2 Score: 50.447 bits (119), Expect = 6.120e-19
Identity = 25/43 (58.14%), Postives = 30/43 (69.77%), Query Frame = -2 
Query:  105 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAE 233
            K    +  +A+MA GK  IIE+AAIVTSG LIEWIGP+   AE
Sbjct:    2 KTLWKHCHIASMAHGKYSIIEDAAIVTSGALIEWIGPQAELAE 44
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEU2 (Imidazolonepropionase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=hutI PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.120e-19
Identity = 28/39 (71.79%), Postives = 31/39 (79.49%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            A L + ++    DL GAWVTPGLIDCHTHTVFGGNRSGE
Sbjct:   40 AALAEPEHDNCIDLDGAWVTPGLIDCHTHTVFGGNRSGE 78

HSP 2 Score: 51.9878 bits (123), Expect = 6.120e-19
Identity = 26/43 (60.47%), Postives = 30/43 (69.77%), Query Frame = -2 
Query:  105 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAE 233
            K    N  +A+MA GK  IIE+AAIVTSG LIEWIGP+   AE
Sbjct:    2 KTLWKNCHIASMAHGKYSIIEDAAIVTSGALIEWIGPQAALAE 44
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEAE (Imidazolonepropionase OS=Pseudomonas aeruginosa GN=hutI PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.750e-18
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AELP  DYA   DL GAW+TPGLIDCHTH VFGGNRSGE
Sbjct:   40 AELPPGDYAERIDLGGAWLTPGLIDCHTHAVFGGNRSGE 78

HSP 2 Score: 43.8986 bits (102), Expect = 1.750e-18
Identity = 21/44 (47.73%), Postives = 26/44 (59.09%), Query Frame = -2 
Query:  102 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAEG 233
            KR   +   AT+  GK  I+E+AA+VT G LI WIGPR     G
Sbjct:    2 KRLWQHCHAATLKGGKYSIVEDAALVTDGPLIHWIGPRAELPPG 45
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEA8 (Imidazolonepropionase OS=Pseudomonas aeruginosa (strain LESB58) GN=hutI PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.750e-18
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AELP  DYA   DL GAW+TPGLIDCHTH VFGGNRSGE
Sbjct:   40 AELPPGDYAERIDLGGAWLTPGLIDCHTHAVFGGNRSGE 78

HSP 2 Score: 43.8986 bits (102), Expect = 1.750e-18
Identity = 21/44 (47.73%), Postives = 26/44 (59.09%), Query Frame = -2 
Query:  102 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAEG 233
            KR   +   AT+  GK  I+E+AA+VT G LI WIGPR     G
Sbjct:    2 KRLWQHCHAATLKGGKYSIVEDAALVTDGPLIHWIGPRAELPPG 45
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEA7 (Imidazolonepropionase OS=Pseudomonas aeruginosa (strain PA7) GN=hutI PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.959e-18
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AELP  DYA   DL GAW+TPGLIDCHTH VFGGNRSGE
Sbjct:   40 AELPPGDYAERIDLGGAWLTPGLIDCHTHAVFGGNRSGE 78

HSP 2 Score: 43.1282 bits (100), Expect = 2.959e-18
Identity = 20/44 (45.45%), Postives = 25/44 (56.82%), Query Frame = -2 
Query:  102 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAEG 233
            KR   +   AT+  GK  I+E+A +VT G LI WIGPR     G
Sbjct:    2 KRLWQHCHAATLRNGKYSIVEDAVLVTDGPLIHWIGPRAELPPG 45
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEAB (Imidazolonepropionase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=hutI PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.847e-18
Identity = 31/39 (79.49%), Postives = 32/39 (82.05%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AELP  DYA   DL GAW+TPGLIDCHTH VFGGNRSGE
Sbjct:   40 AELPPDDYAERIDLDGAWLTPGLIDCHTHAVFGGNRSGE 78

HSP 2 Score: 42.743 bits (99), Expect = 3.847e-18
Identity = 20/38 (52.63%), Postives = 25/38 (65.79%), Query Frame = -2 
Query:  120 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPR 233
            KR   +   AT+  GK  I+E+AA+VT G LI WIGPR
Sbjct:    2 KRLWQHCHAATLKGGKYSIVEDAALVTDGPLIHWIGPR 39
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEF5 (Imidazolonepropionase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=hutI PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 6.503e-18
Identity = 31/41 (75.61%), Postives = 33/41 (80.49%), Query Frame = -3 
Query:    2 RVAELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 124
            R +E P  DYA  +DL GAWVTPG IDCHTHTVFGGNRSGE
Sbjct:   38 RRSEAPAGDYAQVNDLAGAWVTPGFIDCHTHTVFGGNRSGE 78

HSP 2 Score: 40.4318 bits (93), Expect = 6.503e-18
Identity = 21/44 (47.73%), Postives = 26/44 (59.09%), Query Frame = -2 
Query:  102 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAEG 233
            K    +  +A+MA G   IIE+AA+VTSG  IEWIG R     G
Sbjct:    2 KTLWQHCNVASMASGVYSIIEDAAMVTSGAHIEWIGRRSEAPAG 45
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSE14 (Imidazolonepropionase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=hutI PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.503e-18
Identity = 28/38 (73.68%), Postives = 31/38 (81.58%), Query Frame = -3 
Query:    2 ELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 115
            EL + ++    DL GAWVTPGLIDCHTHTVFGGNRSGE
Sbjct:   41 ELAEPEHDNCIDLGGAWVTPGLIDCHTHTVFGGNRSGE 78

HSP 2 Score: 48.521 bits (114), Expect = 6.503e-18
Identity = 25/43 (58.14%), Postives = 28/43 (65.12%), Query Frame = -2 
Query:  105 KRFGNNAKLATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTAE 233
            K    +  +A+MA GK  IIE AAIVTSG LIEWIGP    AE
Sbjct:    2 KTLWKHCHIASMAHGKYSIIENAAIVTSGALIEWIGPETELAE 44
[Close]
BLAST of EY674945 vs. ExPASy Swiss-Prot
Match: HUTI_PSEPU (Imidazolonepropionase OS=Pseudomonas putida GN=hutI PE=3 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 6.926e-18
Identity = 29/39 (74.36%), Postives = 30/39 (76.92%), Query Frame = -3 
Query:    2 AELPKADYATTHDLQGAWVTPGLIDCHTHTVFGGNRSGE 118
            AEL   D   T DL GAW+TPGLIDCHTH VFGGNRSGE
Sbjct:   40 AELAPVDADRTVDLGGAWITPGLIDCHTHAVFGGNRSGE 78

HSP 2 Score: 45.0542 bits (105), Expect = 6.926e-18
Identity = 22/34 (64.71%), Postives = 26/34 (76.47%), Query Frame = -2 
Query:  108 LATMAQGK*LIIEEAAIVTSGTLIEWIGPRGRTA 209
            +ATMA+G+   IE+AAIVTS  LIEWIGPR   A
Sbjct:   10 VATMAEGRYSAIEDAAIVTSAGLIEWIGPRAELA 43

HSP 3 Score: 22.3274 bits (46), Expect = 6.926e-18
Identity = 7/17 (41.18%), Postives = 10/17 (58.82%), Query Frame = -1 
Query:  187 MKTVWEQCQVGNHGPGQ 237
            M+TVW+ C V     G+
Sbjct:    1 MRTVWQHCHVATMAEGR 17
The following BLAST results are available for this feature:
BLAST of EY674945 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18
Match NameE-valueIdentityDescription
HUTI_PSEPF1.884e-2176.92Imidazolonepropionase OS=Pseudomonas fluorescens (... [more]
HUTI_PSESM6.120e-1974.36Imidazolonepropionase OS=Pseudomonas syringae pv. ... [more]
HUTI_PSEU26.120e-1971.79Imidazolonepropionase OS=Pseudomonas syringae pv. ... [more]
HUTI_PSEAE1.750e-1879.49Imidazolonepropionase OS=Pseudomonas aeruginosa GN... [more]
HUTI_PSEA81.750e-1879.49Imidazolonepropionase OS=Pseudomonas aeruginosa (s... [more]
HUTI_PSEA72.959e-1879.49Imidazolonepropionase OS=Pseudomonas aeruginosa (s... [more]
HUTI_PSEAB3.847e-1879.49Imidazolonepropionase OS=Pseudomonas aeruginosa (s... [more]
HUTI_PSEF56.503e-1875.61Imidazolonepropionase OS=Pseudomonas fluorescens (... [more]
HUTI_PSE146.503e-1873.68Imidazolonepropionase OS=Pseudomonas syringae pv. ... [more]
HUTI_PSEPU6.926e-1874.36Imidazolonepropionase OS=Pseudomonas putida GN=hut... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-003-E11-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674945 ID=EY674945; Name=EY674945; organism=Citrus sinensis; type=EST; length=606bp
ACTCGCCGCTGCGATTGCCGCCGAACACCGTGTGGGTGTGGCAGTCGATC
AGCCCGGGCGTGACCCACGCCCCCTGCAGGTCATGGGTGGTCGCGTAGTC
CGCCTTCGGCAGTTCGGCCACGCGGACCGATCCACTCGATGAGCGTGCCC
GAGGTCACGATGGCGGCTTCCTCAATGATCAATTATTTGCCCTGGGCCAT
GGTTGCCAACTTGGCATTGTTCCCAAACCGTTTTCATCAACGCCCCCGGT
GGGTTTATCGATGAAAAAAAACCCGGGGCGTTATGGAACCTTGGCTGGCT
TTGCCCGGGGGCGGGGTTTGACCCACAGCAAAAAGGGAAACAATTCACGA
AAAAAATTCCAAACCGGGCCCCACCATAAAAAAAACCCCGGGGGGGTCCG
GGAAAAATAACCCCAACACACCAGAAAAAAAAAAAAAACAGAAAAAAAAA
AGGGGGGCCCCCCTCTAAGAAGGAAACAAACCTAAGGTTTCCCCGGGGGG
TGGGCCCCAGGGGTATGTGCCCCCCTTTTTTGAGGGGGCAACCCCAATAT
TTTAATATATTCCATGGAGGCCCGGGTCTTTTATTTATAAACATTTTTTT
TGTGGT
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