EY674869

Overview
NameEY674869
Unique NameEY674869
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1020
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK3_ARATH (Probable fructokinase-3 OS=Arabidopsis thaliana GN=At1g06020 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 7.195e-15
Identity = 43/105 (40.95%), Postives = 60/105 (57.14%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPTV                +   + P F++APGG P+NVAI+  RLGGRAAF+GK+G DDFG  L  ++ K  V  + + FDE  +TA  ++
Sbjct:   10 LIVSFGEMLIDFVPTVS--------------GVSLSESPGFLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDDFGHMLAGILRKNGVDDQGINFDEGARTALAFV 100

HSP 2 Score: 24.6386 bits (52), Expect = 7.195e-15
Identity = 20/75 (26.67%), Postives = 35/75 (46.67%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNS-EVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGK 761
            N+ ++R AK+FH+ S  ++  P        K +  +K+ G  L Y  + +   WP  E A  +    W K ++ K
Sbjct:  128 NLELIRSAKVFHYGSISLITEPCRSAHM--KAMEVAKEAGALLSYDPNLREPLWPSPEEARTQIMSIWDKADIIK 200
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK4_ARATH (Probable fructokinase-4 OS=Arabidopsis thaliana GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.468e-14
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPTV                +   D P F++APGG P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ +  V    + FD   +TA  ++
Sbjct:   10 LIVSFGEMLIDFVPTVS--------------GVSLADAPGFIKAPGGAPANVAIAISRLGGRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDTGARTALAFV 100

HSP 2 Score: 25.409 bits (54), Expect = 3.468e-14
Identity = 22/74 (29.73%), Postives = 33/74 (44.59%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNSEVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGK 761
            N+ V+R AK+FH+ S  L V         K +  +K+ G  L Y  + +   WP  E A  +    W K  + K
Sbjct:  128 NLDVIRSAKVFHYGSISLIVEPCR-SAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIK 200
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK2_SOLHA (Fructokinase-2 OS=Solanum habrochaites GN=FRK2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 9.877e-14
Identity = 40/105 (38.10%), Postives = 59/105 (56.19%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPTV                +   + P F++APGG P+NVAI+  RLGGR+AF+GK+G D+FG  L  ++    VQ   + FD+  +TA  ++
Sbjct:   11 LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLGGRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDKGARTALAFV 101

HSP 2 Score: 25.409 bits (54), Expect = 9.877e-14
Identity = 23/87 (26.44%), Postives = 38/87 (43.68%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNSEVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGKEAKGFLGGVRGKN 800
            N+ ++R AK+FH+ S  L V         K +  +K+ G  L Y  + +   WP  E A  +    W   ++ K +   L  + G N
Sbjct:  129 NLDLIRSAKVFHYGSISLIVEPCRAAHM-KAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTGSN 214
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK2_SOLLC (Fructokinase-2 OS=Solanum lycopersicum GN=FRK2 PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 1.283e-13
Identity = 39/105 (37.14%), Postives = 59/105 (56.19%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPTV                +   + P F++APGG P+NVAI+  RLGG++AF+GK+G D+FG  L  ++    VQ   + FD+  +TA  ++
Sbjct:   11 LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLGGKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDKGARTALAFV 101

HSP 2 Score: 25.409 bits (54), Expect = 1.283e-13
Identity = 23/87 (26.44%), Postives = 38/87 (43.68%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNSEVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGKEAKGFLGGVRGKN 800
            N+ ++R AK+FH+ S  L V         K +  +K+ G  L Y  + +   WP  E A  +    W   ++ K +   L  + G N
Sbjct:  129 NLDLIRSAKVFHYGSISLIVEPCRAAHM-KAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLTGSN 214
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK1_ARATH (Probable fructokinase-1 OS=Arabidopsis thaliana GN=At2g31390 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 4.746e-13
Identity = 39/105 (37.14%), Postives = 58/105 (55.24%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPT                 +   + P F++APGG P+NVAI+  RLGGR+AF+GK+G D+FG  L  ++ K  V  + + FD   +TA  ++
Sbjct:    9 LIVSFGEMLIDFVPTES--------------GVSLAEAPGFLKAPGGAPANVAIAVSRLGGRSAFVGKLGDDEFGHMLAGILRKNGVDDQGINFDTGARTALAFV 99

HSP 2 Score: 25.7942 bits (55), Expect = 4.746e-13
Identity = 24/87 (27.59%), Postives = 38/87 (43.68%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNSEVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGKEAKGFLGGVRGKN 800
            N+ ++R AK+FH+ S  L V         K +  +K+ G  L Y  + +   WP  E A  +    W K  + K +   L  + G N
Sbjct:  127 NLDLIRSAKVFHYGSISLIVEPCR-SAHLKAMEVAKEAGALLSYDPNLREPLWPSKEEAKTQIMSIWDKAEIIKVSDVELEFLTGSN 212
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK_SOLTU (Fructokinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.351e-12
Identity = 39/105 (37.14%), Postives = 59/105 (56.19%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            LI  FG +  +FVPTV                +   + P F++APGG P+NVAI+  RLGG++AF+GK+G D+FG  L  ++    VQ   + FD+  +TA  ++
Sbjct:   12 LIVSFGEMLIDFVPTVS--------------GVSLAEAPGFLKAPGGAPANVAIAVTRLGGKSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDKGARTALAFV 102

HSP 2 Score: 22.7126 bits (47), Expect = 1.351e-12
Identity = 7/15 (46.67%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNS 587
            N+ ++R AK+FH+ S
Sbjct:  130 NLDLIRSAKVFHYGS 144
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK2_ARATH (Probable fructokinase-2 OS=Arabidopsis thaliana GN=At1g06030 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.957e-12
Identity = 31/65 (47.69%), Postives = 46/65 (70.77%), Query Frame = 2 
Query:  266 FVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            F++APGG P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ K  V  + + FD+  +TA  ++
Sbjct:   37 FLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDEFGHMLAGILRKNDVDDQGINFDKGARTALAFV 101

HSP 2 Score: 23.8682 bits (50), Expect = 2.957e-12
Identity = 20/75 (26.67%), Postives = 35/75 (46.67%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNS-EVLNVPTMGFKPS*KQLNGSKKFGRAL-YFFDFKFARWPLGEIAGVENPGNWFKKNLGK 761
            N+ ++R AK+FH+ S  ++  P        K +  +K+ G  L Y  + +   WP  E A  +    W K ++ K
Sbjct:  129 NLELIRSAKVFHYGSISLITEPCRSAHM--KAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIK 201
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK1_ORYSJ (Fructokinase-1 OS=Oryza sativa subsp. japonica GN=FRK1 PE=1 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 5.234e-11
Identity = 40/105 (38.10%), Postives = 56/105 (53.33%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            L+  FG +  +FVPTV                +   + P FV+APGG P+NVAI+  RLGG AAF+GK+G D+FG  L  ++    V    V FD   +TA  ++
Sbjct:    7 LVVSFGEMLIDFVPTVA--------------GVSLAEAPAFVKAPGGAPANVAIAVARLGGGAAFVGKLGDDEFGRMLAAILRDNGVDDGGVVFDAGARTALAFV 97
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK1_ORYSI (Fructokinase-1 OS=Oryza sativa subsp. indica GN=FRK1 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.234e-11
Identity = 40/105 (38.10%), Postives = 56/105 (53.33%), Query Frame = 2 
Query:  146 LICCFGAVQKEFVPTVRVHDNQMHPDIYSTWKMVQWDPPEFVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            L+  FG +  +FVPTV                +   + P FV+APGG P+NVAI+  RLGG AAF+GK+G D+FG  L  ++    V    V FD   +TA  ++
Sbjct:    7 LVVSFGEMLIDFVPTVA--------------GVSLAEAPAFVKAPGGAPANVAIAVARLGGGAAFVGKLGDDEFGRMLAAILRDNGVDDGGVVFDAGARTALAFV 97
[Close]
BLAST of EY674869 vs. ExPASy Swiss-Prot
Match: SCRK5_ARATH (Putative fructokinase-5 OS=Arabidopsis thaliana GN=At4g10260 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 8.803e-11
Identity = 30/65 (46.15%), Postives = 45/65 (69.23%), Query Frame = 2 
Query:  266 FVRAPGGPPSNVAISHVRLGGRAAFLGKVGADDFGDELVFMMNKERVQTRAVKFDENVKTACTYM 460
            F++APGG P+NVA +  +LGG++AF+GK G D+FG  LV ++ K  V +  V FD N +TA  ++
Sbjct:   32 FLKAPGGAPANVACAITKLGGKSAFIGKFGDDEFGHMLVNILKKNGVNSEGVCFDTNARTALAFV 96

HSP 2 Score: 21.1718 bits (43), Expect = 8.803e-11
Identity = 7/15 (46.67%), Postives = 12/15 (80.00%), Query Frame = 3 
Query:  543 NVPVLREAKIFHFNS 587
            N  ++++AKIFH+ S
Sbjct:  124 NKDLIKKAKIFHYGS 138
The following BLAST results are available for this feature:
BLAST of EY674869 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10
Match NameE-valueIdentityDescription
SCRK3_ARATH7.195e-1540.95Probable fructokinase-3 OS=Arabidopsis thaliana GN... [more]
SCRK4_ARATH3.468e-1439.05Probable fructokinase-4 OS=Arabidopsis thaliana GN... [more]
SCRK2_SOLHA9.877e-1438.10Fructokinase-2 OS=Solanum habrochaites GN=FRK2 PE=... [more]
SCRK2_SOLLC1.283e-1337.14Fructokinase-2 OS=Solanum lycopersicum GN=FRK2 PE=... [more]
SCRK1_ARATH4.746e-1337.14Probable fructokinase-1 OS=Arabidopsis thaliana GN... [more]
SCRK_SOLTU1.351e-1237.14Fructokinase OS=Solanum tuberosum PE=2 SV=1[more]
SCRK2_ARATH2.957e-1247.69Probable fructokinase-2 OS=Arabidopsis thaliana GN... [more]
SCRK1_ORYSJ5.234e-1138.10Fructokinase-1 OS=Oryza sativa subsp. japonica GN=... [more]
SCRK1_ORYSI5.234e-1138.10Fructokinase-1 OS=Oryza sativa subsp. indica GN=FR... [more]
SCRK5_ARATH8.803e-1146.15Putative fructokinase-5 OS=Arabidopsis thaliana GN... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C1-401-002-E06-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674869 ID=EY674869; Name=EY674869; organism=Citrus sinensis; type=EST; length=1020bp
ACTGCGACGAAGAAAACCCCTTTGGCGTCCGAAGAAAACCCAAGTCGAGA
ATGGCTTAATTGAGACCAAAAAAGAAACGAAAGAACTAAGCGACGGCGTA
TTGGATTACGATGACGACATGAACTTTCCTTTCGATGATCCTCCTCTAAT
ATGCTGCTTCGGCGCCGTACAAAAAGAGTTTGTGCCGACGGTGAGAGTGC
ACGACAACCAGATGCACCCGGACATCTACTCCACGTGGAAGATGGTCCAG
TGGGATCCGCCGGAGTTTGTGAGGGCCCCAGGTGGGCCCCCTTCCAACGT
GGCGATATCCCACGTGAGGCTTGGCGGGAGGGCGGCGTTCTTGGGGAAGG
TTGGCGCCGATGACTTCGGCGATGAGTTGGTTTTTATGATGAATAAAGAG
AGAGTGCAGACACGCGCGGTGAAGTTTGATGAGAATGTTAAGACCGCGTG
TACGTATATGGAAGATTAAGTTTGAGAATGGGAAGATGATGGCTGAGACG
GTGAAAGATTCGGCTGAGGATTCGCTTCTGAGCTCTGATTTGAACGTACC
AGTGCTCAGAGAGGCTAAAATTTTCCACTTCAATTCTGAAGTTTTGAATG
TCCCCACAATGGGGTTCAAACCTTCTTAAAAGCAATTGAATGGGTCTAAG
AAATTTGGGAGGGCTTTATATTTTTTTGATTTTAAATTTGCCCGTTGGCC
TTTGGGGGAAATCGCGGGGGTGGAAAACCCGGGGAACTGGTTTAAAAAAA
ACCTGGGAAAGGAGGCCAAAGGTTTTTTGGGGGGGGTCAGGGGCAAAAAC
CTGGGAATTTTCTTTTATAAAGGAAAAATTTTTTTATGAAAAGGAAAAAA
AAAAATTTTTTTTTCCCCCAATTTTTTTTTGGGGGAAAAAATTTTGAAAA
AAAAACATAAAAAAAACCCCGGGGGGGGGATTTTTTATTCTTATTTATTT
ACCCCCCGGGGGGGGGGAGAAAAAACCCCCTCCCCCCCTGTGTGGGGGGG
GGGGAAGGGGTGAGGGGGGA
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