EY674853

Overview
NameEY674853
Unique NameEY674853
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1000
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY674853_ssr218 microsatellite EY674853_ssr218:218..229. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH6_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 6 OS=Arabidopsis thaliana GN=XTH6 PE=2 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 9.320e-29
Identity = 80/180 (44.44%), Postives = 107/180 (59.44%), Query Frame = 2 
Query:  155 RPATFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYYEFPTPKM*ETSLILSSWETERVNLTRSKPI-----IYANGKGEKRTENEPLVLPLLAIIHLYTNLLGTFSILAFLGERMGPPRGLKKTMERAPFFPMNPTPWGGRSNLME 679
            RPATF+EDFK  WS++H+RQ+E G+AIQLVLDQ++GCGFASKR+YLFGRVSMKIKL+PGDSAGTVTAFY         +T+ +    + E +     +P      I+A+GKG+ R +   L        H YT L     I+ ++ +   P R  K    +   +P +  P G  S L E
Sbjct:   31 RPATFVEDFKAAWSESHIRQMEDGKAIQLVLDQSTGCGFASKRKYLFGRVSMKIKLIPGDSAGTVTAFYMNS------DTATVRDELDFEFLGNRSGQPYSVQTNIFAHGKGD-REQRVNLWFDPSMDYHTYTILWSHKHIVFYVDD--VPIREYKNNEAKNIAYPTS-QPMGVYSTLWE 200
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH7_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 7 OS=Arabidopsis thaliana GN=XTH7 PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 1.985e-28
Identity = 61/114 (53.51%), Postives = 79/114 (69.30%), Query Frame = 2 
Query:  155 RPATFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYYEFPTPKM*ETSLILSSWETERVNLTRSKPI-----IYANGKGEK 481
            RPA F +DF++ WSD H+ QI+GGRAIQL LD +SGCGFASK+QYLFGRVSMKIKL+PGDSAGTVTAFY         +T  +    + E +     +P      ++A+GKG++
Sbjct:   30 RPAKFEDDFRIAWSDTHITQIDGGRAIQLKLDPSSGCGFASKKQYLFGRVSMKIKLIPGDSAGTVTAFYMNS------DTDSVRDELDFEFLGNRSGQPYTVQTNVFAHGKGDR 137

HSP 2 Score: 26.9498 bits (58), Expect = 1.985e-28
Identity = 12/26 (46.15%), Postives = 15/26 (57.69%), Query Frame = 3 
Query:  471 KAKKEQRMNLWFCPCLQLSTFTLISW 548
            K  +EQR+NLWF P      +  ISW
Sbjct:  134 KGDREQRVNLWFDPSRDFHEYA-ISW 158
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH12_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 12 OS=Arabidopsis thaliana GN=XTH12 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.082e-20
Identity = 34/66 (51.52%), Postives = 46/66 (69.70%), Query Frame = 2 
Query:  164 TFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFY 361
            +F + F +TW        E G+ +   LD+ SG GF SK++YLFG++ MKIKLVPG+SAGTVTA+Y
Sbjct:   26 SFYDSFDITWGAGRANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPGNSAGTVTAYY 91

HSP 2 Score: 37.3502 bits (85), Expect = 1.082e-20
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 1 
Query:  391 DELDFEFLGNRTGQPYPVQT 450
            DE+DFEFLGN TGQPY + T
Sbjct:  100 DEIDFEFLGNVTGQPYVIHT 119

HSP 3 Score: 25.7942 bits (55), Expect = 1.082e-20
Identity = 10/28 (35.71%), Postives = 15/28 (53.57%), Query Frame = 3 
Query:  471 KAKKEQRMNLWFCPCLQLSTFTLISWEP 554
            K  +E +  LWF P     T+T++ W P
Sbjct:  126 KGNREMQFYLWFDPTADFHTYTVL-WNP 152
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH25_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana GN=XTH25 PE=2 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 5.130e-20
Identity = 33/66 (50.00%), Postives = 48/66 (72.73%), Query Frame = 2 
Query:  164 TFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFY 361
            TF  +F +TW D   + +  G  + L LD+ SG GF +K++YLFG++ M++KLVPG+SAGTVTA+Y
Sbjct:   29 TFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGNSAGTVTAYY 94

HSP 2 Score: 35.4242 bits (80), Expect = 5.130e-20
Identity = 14/20 (70.00%), Postives = 16/20 (80.00%), Query Frame = 1 
Query:  391 DELDFEFLGNRTGQPYPVQT 450
            DE+DFEFLGN TG PY + T
Sbjct:  103 DEIDFEFLGNLTGDPYTMHT 122

HSP 3 Score: 26.9498 bits (58), Expect = 5.130e-20
Identity = 10/28 (35.71%), Postives = 17/28 (60.71%), Query Frame = 3 
Query:  471 KAKKEQRMNLWFCPCLQLSTFTLISWEP 554
            K  +EQ+ +LWF P     T++++ W P
Sbjct:  129 KGDREQQFHLWFDPTADFHTYSVL-WNP 155
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: BRU1_SOYBN (Brassinosteroid-regulated protein BRU1 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 8.617e-20
Identity = 35/72 (48.61%), Postives = 49/72 (68.06%), Query Frame = 2 
Query:  164 TFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYYEFPTP 379
            +F +DF +TW     +   GG+ + L LD+ SG GF SK++YLFGR+ M++KLV G+SAGTVTA+Y     P
Sbjct:   31 SFYQDFDLTWGGDRAKIFNGGQLLSLSLDKVSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGP 102

HSP 2 Score: 33.8834 bits (76), Expect = 8.617e-20
Identity = 14/33 (42.42%), Postives = 21/33 (63.64%), Query Frame = 1 
Query:  391 DELDFEFLGNRTGQPYPVQTYYLCKWKRRKKNR 489
            DE+DFEFLGN +G PY + T    + K  ++ +
Sbjct:  105 DEIDFEFLGNLSGDPYILHTNIFTQGKGNREQQ 137

HSP 3 Score: 27.335 bits (59), Expect = 8.617e-20
Identity = 11/28 (39.29%), Postives = 17/28 (60.71%), Query Frame = 3 
Query:  471 KAKKEQRMNLWFCPCLQLSTFTLISWEP 554
            K  +EQ+  LWF P     T+++I W+P
Sbjct:  131 KGNREQQFYLWFDPTRNFHTYSII-WKP 157
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH13_ARATH (Putative xyloglucan endotransglucosylase/hydrolase protein 13 OS=Arabidopsis thaliana GN=XTH13 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.117e-19
Identity = 32/66 (48.48%), Postives = 48/66 (72.73%), Query Frame = 2 
Query:  164 TFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFY 361
            +F ++F +TW +     +E G+ +   LD+ SG GF SK++YLFG++ MK+KLV G+SAGTVTA+Y
Sbjct:   25 SFYDNFDITWGNGRANIVESGQLLTCTLDKISGSGFQSKKEYLFGKIDMKMKLVAGNSAGTVTAYY 90

HSP 2 Score: 36.5798 bits (83), Expect = 1.117e-19
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 1 
Query:  391 DELDFEFLGNRTGQPYPVQT 450
            DE+DFEFLGN TGQPY + T
Sbjct:   99 DEIDFEFLGNVTGQPYVLHT 118

HSP 3 Score: 25.7942 bits (55), Expect = 1.117e-19
Identity = 10/28 (35.71%), Postives = 15/28 (53.57%), Query Frame = 3 
Query:  471 KAKKEQRMNLWFCPCLQLSTFTLISWEP 554
            K  +E +  LWF P     T+T++ W P
Sbjct:  125 KGNREMQFYLWFDPTADFHTYTVL-WNP 151
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH14_ARATH (Xyloglucan endotransglucosylase/hydrolase protein 14 OS=Arabidopsis thaliana GN=XTH14 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.649e-19
Identity = 33/65 (50.77%), Postives = 45/65 (69.23%), Query Frame = 2 
Query:  167 FLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFY 361
            F E F +TW +      E G+ +   LD+ SG GF SK++YLFG++ MK+KLV G+SAGTVTA+Y
Sbjct:   30 FYESFDITWGNGRANIFENGQLLTCTLDKVSGSGFQSKKEYLFGKIDMKLKLVAGNSAGTVTAYY 94

HSP 2 Score: 41.2022 bits (95), Expect = 3.649e-19
Identity = 29/88 (32.95%), Postives = 42/88 (47.73%), Query Frame = 1 
Query:  391 DELDFEFLGNRTGQPYPVQTYYLCKWKRRKKNRE*TSGFAPACNYP--PLH*SPGNLLHFGFPRGKNGPTQGFKKNNGKGSLFP-NEP 645
            DE+DFEFLGNRTG PY + T      K   +  +    F P  ++    +H +P N++          P + FK N   G  +P N+P
Sbjct:  103 DEIDFEFLGNRTGHPYTIHTNVFTGGK-GDREMQFRLWFDPTADFHTYTVHWNPVNIIFL----VDGIPIRVFKNNEKNGVAYPKNQP 185
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH8_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 8 OS=Arabidopsis thaliana GN=XTH8 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.043e-18
Identity = 32/66 (48.48%), Postives = 43/66 (65.15%), Query Frame = 2 
Query:  164 TFLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFY 361
            +F ++F + WS+ H    + G    L LD ++GCGF +K  Y FG  SMK+KLV GDSAG VTA+Y
Sbjct:   24 SFEDNFNIMWSENHFTTSDDGEIWNLSLDNDTGCGFQTKHMYRFGWFSMKLKLVGGDSAGVVTAYY 89

HSP 2 Score: 44.2838 bits (103), Expect = 1.043e-18
Identity = 22/35 (62.86%), Postives = 25/35 (71.43%), Query Frame = 1 
Query:  388 RDELDFEFLGNRTGQPYPVQTYYLCKWKRRKKNRE 492
            RDE+DFEFLGNRTGQPY +QT     +K    NRE
Sbjct:  100 RDEIDFEFLGNRTGQPYIIQTNV---YKNGTGNRE 131
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH10_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 10 OS=Arabidopsis thaliana GN=XTH10 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.186e-18
Identity = 34/71 (47.89%), Postives = 44/71 (61.97%), Query Frame = 2 
Query:  167 FLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYYEFPTP 379
            F +DF VTWS  H+     GR+  L LDQ SG  F+S + +LFG++ MKIKL+ G S GTV A+Y     P
Sbjct:   37 FNKDFFVTWSPTHVNTSNDGRSRTLKLDQESGASFSSIQTFLFGQIDMKIKLIRGSSQGTVVAYYMSSDQP 107

HSP 2 Score: 38.1206 bits (87), Expect = 4.186e-18
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 1 
Query:  388 RDELDFEFLGNRTGQPYPVQT 450
            RDE+DFEFLGN  GQPY +QT
Sbjct:  109 RDEIDFEFLGNVNGQPYILQT 129

HSP 3 Score: 24.2534 bits (51), Expect = 4.186e-18
Identity = 7/21 (33.33%), Postives = 15/21 (71.43%), Query Frame = 3 
Query:  480 KEQRMNLWFCPCLQLSTFTLI 542
            +E+R++LWF P     T++++
Sbjct:  139 REERIHLWFDPAKDFHTYSIL 159
[Close]
BLAST of EY674853 vs. ExPASy Swiss-Prot
Match: XTH_TOBAC (Probable xyloglucan endotransglucosylase/hydrolase protein OS=Nicotiana tabacum GN=XTH PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.263e-17
Identity = 55/159 (34.59%), Postives = 84/159 (52.83%), Query Frame = 2 
Query:  167 FLEDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYYEFPTPKM*ETSLILSSWETERVNLTRSKPIIYANGKGEKRTENEPLVLPLLAIIHLYTNLLGTFSILAFLGERMGPPRGLKKTMERAPFFPMN 643
            F ++++ +W+  H++ + GG  + LVLD++SG GF SK+ YLFG  SMK+KLV GDSAG VTAFY      +  E         T +  + ++   ++  GKG+ R +   L        H Y+ L  TF I+ F+ +   P R  K + +    FP N
Sbjct:   32 FWKNYEPSWASHHIKYLSGGSTVDLVLDRSSGAGFQSKKSYLFGHFSMKLKLVGGDSAGVVTAFYLSSNNAEHDEIDFEFLGNRTGQPYILQTN--VFTGGKGD-REQRIYLWFDPTKGYHSYSVLWNTFQIVIFVDD--VPIRAFKNSKDLGVKFPFN 185
The following BLAST results are available for this feature:
BLAST of EY674853 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 32
Match NameE-valueIdentityDescription
XTH6_ARATH9.320e-2944.44Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH7_ARATH1.985e-2853.51Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH12_ARATH1.082e-2051.52Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH25_ARATH5.130e-2050.00Probable xyloglucan endotransglucosylase/hydrolase... [more]
BRU1_SOYBN8.617e-2048.61Brassinosteroid-regulated protein BRU1 OS=Glycine ... [more]
XTH13_ARATH1.117e-1948.48Putative xyloglucan endotransglucosylase/hydrolase... [more]
XTH14_ARATH3.649e-1950.77Xyloglucan endotransglucosylase/hydrolase protein ... [more]
XTH8_ARATH1.043e-1848.48Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH10_ARATH4.186e-1847.89Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH_TOBAC1.263e-1734.59Probable xyloglucan endotransglucosylase/hydrolase... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-002-D02-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674853 ID=EY674853; Name=EY674853; organism=Citrus sinensis; type=EST; length=1000bp
CATAACCCTACATATCCCCTTTAGATCTCTTTCTAATTAGTAACAGATAA
TATTTTATTGTACGGCTTGTTTTACAGCTATGGCTATCCTAAGAAATGCA
ATAGCATTATTTGTGTTGCTTTACTGTCTTGTATCCTGCTCTTGCGTTTT
AGGGCGACCGGCTACTTTTCTTGAGGATTTTAAAGTCACATGGTCCGATG
CCCATCTCAGGCAAATCGAGGGAGGGAGGGCCATCCAGCTAGTTCTAGAC
CAAAATTCAGGATGTGGGTTTGCCTCCAAAAGGCAGTACTTGTTCGGGCG
TGTAAGCATGAAGATCAAGCTCGTTCCTGGAGACTCTGCTGGAACTGTCA
CTGCCTTTTATTATGAATTCCCAACACCGAAAATGTGAGAGACGAGCTTG
ATTTTGAGTTCCTGGGAAACCGAACGGGTCAACCTTACCCGGTCCAAACC
TATTATCTATGCAAATGGAAAAGGCGAAAAAAGAACAGAGAATGAACCTC
TGGTTTTGCCCCTGCTTGCAATTATCCACCTTTACACTAATCTCCTGGGA
ACCTTCTCCATTTTGGCTTTCCTAGGGGAAAGAATGGGCCCACCCAGGGG
TTTAAAAAAAACAATGGAAAGGGCTCCCTTTTTCCCAATGAACCCCACCC
CATGGGGGGGGAGATCCAACCTCATGGAGAATTCCAAATAACTGGGGCAA
CAAAAAGTGGGATCTATATAAAAAAATAATTGGGAACAAAAAACCCCCCT
TTTTTTATCTTAATTTCACAAAAAATTTTTAAATATAAAAAGGGGTACCC
CAAAATTCACTTGACACAAAAATAAAAGTGTGGGCCCTCACAAAAACCAG
AGGTTGTAATTGGGGGGGGGAGAGGCAAAATAATAATTTTATTCAACCCC
CTCCCTTCCCTCACCCAATGAGGGACGGACAAACTAAAAATAAAAATAAA
ACGAAAGAGTTGGGGGTATACTGACTCTCCTTTAACCCCCCTCTAACATG
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