EY655719

Overview
NameEY655719
Unique NameEY655719
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length836
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_ARATH (Pre-mRNA-splicing factor SF2 OS=Arabidopsis thaliana GN=SF2 PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.664e-36
Identity = 56/66 (84.85%), Postives = 62/66 (93.94%), Query Frame = 3 
Query:   66 MSSRASRTLYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            MSSR+SRT+YVGNLPGDIREREVEDLF KYGP+  IDLK+PPRPPGYAFVEF++ARDAEDAI GRD
Sbjct:    1 MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAEDAIHGRD 66

HSP 2 Score: 35.039 bits (79), Expect = 9.664e-36
Identity = 25/72 (34.72%), Postives = 27/72 (37.50%), Query Frame = 2 
Query:  260 RXXYDFDGHRLRVELA----------XXXXXXXXXXXXXXXXXXXXXXXXXXXEYRVLVTWITLFCLWQDLK 445
            R  YDFDGHRLRVELA                                     E+RVLVT +     WQDLK
Sbjct:   65 RDGYDFDGHRLRVELAHGGRRSSDDTRGSFNGGGRGGGRGRGDGGSRGPSRRSEFRVLVTGLPSSASWQDLK 136

HSP 3 Score: 34.6538 bits (78), Expect = 9.664e-36
Identity = 14/19 (73.68%), Postives = 15/19 (78.95%), Query Frame = 1 
Query:  442 KDHMRXAGDVCFLPVFRDA 498
            KDHMR  GDVCF  V+RDA
Sbjct:  136 KDHMRKGGDVCFSQVYRDA 154
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFR1B_DANRE (Splicing factor, arginine/serine-rich 1B OS=Danio rerio GN=sfrs1b PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.439e-21
Identity = 38/58 (65.52%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR ++VED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   17 IYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 74

HSP 2 Score: 35.039 bits (79), Expect = 1.439e-21
Identity = 14/18 (77.78%), Postives = 15/18 (83.33%), Query Frame = 1 
Query:  442 KDHMRXAGDVCFLPVFRD 495
            KDHMR AGDVC+  VFRD
Sbjct:  137 KDHMREAGDVCYADVFRD 154

HSP 3 Score: 29.6462 bits (65), Expect = 1.439e-21
Identity = 20/67 (29.85%), Postives = 26/67 (38.81%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVEL---AXXXXXXXXXXXXXXXXXXXXXXXXXXXEYRVLVTWITLFCLWQDLK 445
            + R  YD+DG+RLRVE                                EYRV+V+ +     WQDLK
Sbjct:   71 YGRDGYDYDGYRLRVEFPRSGRGGGRGGGGGGGVGAPRGRYGPPSRRSEYRVIVSGLPPSGSWQDLK 137
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_CHICK (Splicing factor, arginine/serine-rich 1 OS=Gallus gallus GN=SFRS1 PE=1 SV=3)

HSP 1 Score: 79.337 bits (194), Expect = 1.862e-21
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 35.039 bits (79), Expect = 1.862e-21
Identity = 14/18 (77.78%), Postives = 15/18 (83.33%), Query Frame = 1 
Query:  442 KDHMRXAGDVCFLPVFRD 495
            KDHMR AGDVC+  VFRD
Sbjct:  138 KDHMREAGDVCYADVFRD 155

HSP 3 Score: 29.6462 bits (65), Expect = 1.862e-21
Identity = 20/67 (29.85%), Postives = 26/67 (38.81%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVEL---AXXXXXXXXXXXXXXXXXXXXXXXXXXXEYRVLVTWITLFCLWQDLK 445
            + R  YD+DG+RLRVE                                EYRV+V+ +     WQDLK
Sbjct:   72 YGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSEYRVIVSGLPPSGSWQDLK 138
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: RSP3_CAEEL (Probable splicing factor, arginine/serine-rich 3 OS=Caenorhabditis elegans GN=rsp-3 PE=1 SV=2)

HSP 1 Score: 83.9593 bits (206), Expect = 7.369e-17
Identity = 38/58 (65.52%), Postives = 49/58 (84.48%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLPGD+RE+EVED+F+KYG I ++D+K   R P +AFVEFE+ RDAEDA+R RD
Sbjct:   11 VYVGNLPGDVREKEVEDIFHKYGRIKYVDIK-SGRGPAFAFVEFEDHRDAEDAVRARD 67

HSP 2 Score: 24.6386 bits (52), Expect = 7.369e-17
Identity = 9/15 (60.00%), Postives = 12/15 (80.00%), Query Frame = 2 
Query:  257 SRXXYDFDGHRLRVE 301
            +R  Y+FDG R+RVE
Sbjct:   65 ARDGYEFDGRRIRVE 79
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_XENTR (Splicing factor, arginine/serine-rich 1 OS=Xenopus tropicalis GN=sfrs1 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.552e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.552e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_PONAB (Splicing factor, arginine/serine-rich 1 OS=Pongo abelii GN=SFRS1 PE=2 SV=3)

HSP 1 Score: 79.337 bits (194), Expect = 3.560e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.560e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_PIG (Splicing factor, arginine/serine-rich 1 OS=Sus scrofa GN=SFRS1 PE=2 SV=3)

HSP 1 Score: 79.337 bits (194), Expect = 3.560e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.560e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_MOUSE (Splicing factor, arginine/serine-rich 1 OS=Mus musculus GN=Sfrs1 PE=1 SV=3)

HSP 1 Score: 79.337 bits (194), Expect = 3.560e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.560e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_HUMAN (Splicing factor, arginine/serine-rich 1 OS=Homo sapiens GN=SFRS1 PE=1 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 3.560e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.560e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
[Close]
BLAST of EY655719 vs. ExPASy Swiss-Prot
Match: SFRS1_BOVIN (Splicing factor, arginine/serine-rich 1 OS=Bos taurus GN=SFRS1 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.560e-16
Identity = 37/58 (63.79%), Postives = 45/58 (77.59%), Query Frame = 3 
Query:   90 LYVGNLPGDIREREVEDLFYKYGPIAHIDLKIPPRPPGYAFVEFEEARDAEDAIRGRD 263
            +YVGNLP DIR +++ED+FYKYG I  IDLK     P +AFVEFE+ RDAEDA+ GRD
Sbjct:   18 IYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRD 75

HSP 2 Score: 26.9498 bits (58), Expect = 3.560e-16
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 2 
Query:  254 WSRXXYDFDGHRLRVE 301
            + R  YD+DG+RLRVE
Sbjct:   72 YGRDGYDYDGYRLRVE 87
The following BLAST results are available for this feature:
BLAST of EY655719 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 14
Match NameE-valueIdentityDescription
SFRS1_ARATH9.664e-3684.85Pre-mRNA-splicing factor SF2 OS=Arabidopsis thalia... [more]
SFR1B_DANRE1.439e-2165.52Splicing factor, arginine/serine-rich 1B OS=Danio ... [more]
SFRS1_CHICK1.862e-2163.79Splicing factor, arginine/serine-rich 1 OS=Gallus ... [more]
RSP3_CAEEL7.369e-1765.52Probable splicing factor, arginine/serine-rich 3 O... [more]
SFRS1_XENTR3.552e-1663.79Splicing factor, arginine/serine-rich 1 OS=Xenopus... [more]
SFRS1_PONAB3.560e-1663.79Splicing factor, arginine/serine-rich 1 OS=Pongo a... [more]
SFRS1_PIG3.560e-1663.79Splicing factor, arginine/serine-rich 1 OS=Sus scr... [more]
SFRS1_MOUSE3.560e-1663.79Splicing factor, arginine/serine-rich 1 OS=Mus mus... [more]
SFRS1_HUMAN3.560e-1663.79Splicing factor, arginine/serine-rich 1 OS=Homo sa... [more]
SFRS1_BOVIN3.560e-1663.79Splicing factor, arginine/serine-rich 1 OS=Bos tau... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-081-F01-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY655719 ID=EY655719; Name=EY655719; organism=Citrus sinensis; type=EST; length=836bp
CCACGCGTCCGCGAAGCTCTTATTCTTTGTGCACAACTATCGTTTAATCA
TCATTACAGGAAAAAATGAGCAGCCGTGCAAGCAGAACTCTCTATGTTGG
GAACCTGCCTGGTGATATTCGGGAGAGAGAAGTGGAAGATCTGTTTTACA
AGTATGGACCTATAGCCCACATTGACCTGAAGATCCCGCCAAGGCCTCCT
GGTTATGCATTTGTTGAGTTTGAAGAGGCTCGTGATGCTGAGGATGCAAT
TCGTGGTCGCGATGNNGCTATGATTTTGATGGGCATCGGTTACGGGTGGA
ACTTGCGCATGGTGGGCGGGGCCGTTCATCATCTGATCGTCATAGTAGTC
ACAGCAGTGGCCGAGGACGTGGAGTATCCAGGCGATCCGAATATCGTGTG
TTAGTCACCTGGATTACCCTCTTCTGCTTATGGCAAGACCTAAAGATCAC
ATGCGTCNAGCAGGAGATGTTTGTTTCTTACCAGTTTTTCGTGATGCAGT
GGCCCACTGCCATTGGGGCCTCCCAAACTTTATTGTTTTAAACTGCAATC
AAAAAACTTGAGGACTTTTAATTTAGGAAAAGCTTTTTTCGGGCTTTTTT
TTTCGGGTGGGGGGTTTTTTCTTAAACCCGGTGGGGGCCCCAAGTCCTTT
TGGGGGGGCCTTTTTTTTTAAAAGGGGGGGGGAGAGACAACACCCCCCTC
ACTCGTGGGGGGGAATAATAATGGGGGGGTGTTTTTNAAAAAGTTTTTCA
AAAAAAAAAAATTTCCCCCGCTTTCTTTTTAAAAACAACAAAAAAGGGGG
GTGTTTTCCCCCCCCCCGGAGAAAAAAAAATTTTTT
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