EY650440

Overview
NameEY650440
Unique NameEY650440
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length786
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYM_FLAPR (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.096e-25
Identity = 40/87 (45.98%), Postives = 52/87 (59.77%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTYPH-MAGSTPVLRQATTSEY 761
            AAG IPSP ++ADVVT TTHKSLRGPRGAM    +GL  +  +GK  +    +K  +     L    + H + G    L+QATT+EY
Sbjct:  267 AAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEY 353

HSP 2 Score: 68.1662 bits (165), Expect = 2.096e-25
Identity = 35/54 (64.81%), Postives = 44/54 (81.48%), Query Frame = 2 
Query:  332 YIIVLKMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            YI   +++KSATLF+P LI AGA+AYARL +Y R RKVC+K  AIMLAD+AHI+
Sbjct:  210 YIDYDQLEKSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAIMLADMAHIS 263
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYM_SOLTU (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.728e-25
Identity = 35/54 (64.81%), Postives = 45/54 (83.33%), Query Frame = 2 
Query:  332 YIIVLKMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            YI   +++KSATLF+P LI AGA+AYARL +Y+R RKVCNK  AI+LAD+AHI+
Sbjct:  210 YIDYDQLEKSATLFRPKLIVAGASAYARLYDYDRIRKVCNKQKAILLADMAHIS 263

HSP 2 Score: 67.0106 bits (162), Expect = 2.728e-25
Identity = 39/87 (44.83%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTYPH-MAGSTPVLRQATTSEY 761
            AAG IPSP ++ADVVT TTHKSLRGPRGAM    +G+  +  +GK  +    +K  +     L    + H + G    L+QATT EY
Sbjct:  267 AAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRKGVKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY 353
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYM_ARATH (Serine hydroxymethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=SHM1 PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 3.550e-25
Identity = 36/54 (66.67%), Postives = 44/54 (81.48%), Query Frame = 2 
Query:  332 YIIVLKMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            YI   +M+KSATLF+P LI AGA+AYARL +Y R RKVCNK  A+MLAD+AHI+
Sbjct:  209 YIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHIS 262

HSP 2 Score: 65.855 bits (159), Expect = 3.550e-25
Identity = 40/87 (45.98%), Postives = 50/87 (57.47%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTYPH-MAGSTPVLRQATTSEY 761
            AA  IPSP ++ADVVT TTHKSLRGPRGAM    +G+  I  +GK       +K  +     L    + H + G    L+QATTSEY
Sbjct:  266 AANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEY 352
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYM_PEA (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.018e-24
Identity = 40/87 (45.98%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTYPH-MAGSTPVLRQATTSEY 761
            AAG IPSP ++ADVVT TTHKSLRGPRGAM    +GL  +  +GK  +    +K  +     L    + H + G    L+QATT EY
Sbjct:  267 AAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY 353

HSP 2 Score: 66.6254 bits (161), Expect = 1.018e-24
Identity = 33/54 (61.11%), Postives = 44/54 (81.48%), Query Frame = 2 
Query:  332 YIIVLKMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            YI   +++KSATLF+P LI AGA+AYARL +Y R RKVC+K  A++LAD+AHI+
Sbjct:  210 YIDYDQLEKSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAVLLADMAHIS 263
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYN_FLAPR (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.921e-24
Identity = 34/54 (62.96%), Postives = 44/54 (81.48%), Query Frame = 2 
Query:  332 YIIVLKMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            YI   +++KSATLF+P LI AGA+AYARL +Y R RKVC+K  AI+LAD+AHI+
Sbjct:  210 YIDYDQLEKSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAILLADMAHIS 263

HSP 2 Score: 66.6254 bits (161), Expect = 2.921e-24
Identity = 39/87 (44.83%), Postives = 51/87 (58.62%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTYPH-MAGSTPVLRQATTSEY 761
            AAG IPSP ++ADVVT TTHKSLRGPRGAM    +G+  +  +GK       +K  +     L    + H + G    L+QATT+EY
Sbjct:  267 AAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEVNKQGKEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEY 353
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYC_ASHGO (Serine hydroxymethyltransferase, cytosolic OS=Ashbya gossypii GN=SHM2 PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 2.863e-16
Identity = 38/92 (41.30%), Postives = 52/92 (56.52%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTY---PH---MAGSTPVLRQATTSEY 761
            AAG IPSP E+AD+VT TTHKSLRGPRGAM    +G+  +  +     M D    I  +    ++P +   PH   ++     L+QATT E+
Sbjct:  228 AAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVHPKTGEEVMYDLEGPINFS----VFPGHQGGPHNHTISALATALKQATTPEF 315

HSP 2 Score: 48.9062 bits (115), Expect = 2.863e-16
Identity = 19/48 (39.58%), Postives = 37/48 (77.08%), Query Frame = 2 
Query:  350 MDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            ++K+A L++P ++ AG +AY RL +Y+R R++ +K+ A ++ D+AHI+
Sbjct:  177 LEKNAVLYRPKILVAGTSAYCRLIDYKRMREIADKVGAYLMVDMAHIS 224
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYM_ASHGO (Serine hydroxymethyltransferase, mitochondrial OS=Ashbya gossypii GN=SHM1 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.717e-16
Identity = 38/92 (41.30%), Postives = 54/92 (58.70%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRGKTCYMIDNNKGIKLAMQDLMWPTY---PH---MAGSTPVLRQATTSEY 761
            AAG  PSP E++D+VT TTHKSLRGPRGAM    +G+  +  +G T  M D +K I  +    ++P +   PH   ++     L+QA T E+
Sbjct:  252 AAGVHPSPFEYSDIVTTTTHKSLRGPRGAMIFYRKGIRKVTKKG-TEIMYDLDKRINFS----VFPAHQGGPHNHTISALAVALKQAATPEF 338

HSP 2 Score: 47.7506 bits (112), Expect = 3.717e-16
Identity = 21/57 (36.84%), Postives = 40/57 (70.18%), Query Frame = 2 
Query:  350 MDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHITEGVSCWRHP 520
            + +++ LF+P +I AG +AYAR+ +Y+R R++ +   A +L+D+AH++  V+   HP
Sbjct:  201 LSETSKLFRPKVIVAGTSAYARVLDYKRFREIADACGAYLLSDMAHVSGLVAAGVHP 257
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYC_CANGA (Serine hydroxymethyltransferase, cytosolic OS=Candida glabrata GN=SHM2 PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.722e-16
Identity = 37/88 (42.05%), Postives = 49/88 (55.68%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINR-GKTC-YMIDNNKGIKLAMQDLMWPTYPHMAGSTPVLRQATTSEY 761
            AAG IPSP E+AD+VT TTHKSLRGPRGAM    +G+  +  + GK   Y ++N     +       P    +A     L+QA T E+
Sbjct:  228 AAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGIRSVNQKTGKEIPYDLENPINFSVFPGHQGGPHNHTIAALATALKQAATPEF 315

HSP 2 Score: 47.7506 bits (112), Expect = 3.722e-16
Identity = 19/48 (39.58%), Postives = 36/48 (75.00%), Query Frame = 2 
Query:  350 MDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            ++K+A L++P ++ AG +AY RL +Y+R R++ +K  A ++ D+AHI+
Sbjct:  177 LEKNAILYRPKILVAGTSAYCRLIDYKRMREIADKCGAYLMVDMAHIS 224
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYC_YEAST (Serine hydroxymethyltransferase, cytosolic OS=Saccharomyces cerevisiae GN=SHM2 PE=1 SV=2)

HSP 1 Score: 58.151 bits (139), Expect = 6.287e-16
Identity = 38/88 (43.18%), Postives = 49/88 (55.68%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINR-GK-TCYMIDNNKGIKLAMQDLMWPTYPHMAGSTPVLRQATTSEY 761
            AAG IPSP E+AD+VT TTHKSLRGPRGAM    +G+  I  + GK   Y ++N     +       P    +A     L+QA T E+
Sbjct:  228 AAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSINPKTGKEVLYDLENPINFSVFPGHQGGPHNHTIAALATALKQAATPEF 315

HSP 2 Score: 47.3654 bits (111), Expect = 6.287e-16
Identity = 19/48 (39.58%), Postives = 36/48 (75.00%), Query Frame = 2 
Query:  350 MDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            ++K+A L++P ++ AG +AY RL +Y+R R++ +K  A ++ D+AHI+
Sbjct:  177 LEKNAILYRPKVLVAGTSAYCRLIDYKRMREIADKCGAYLMVDMAHIS 224
[Close]
BLAST of EY650440 vs. ExPASy Swiss-Prot
Match: GLYC_CAEBR (Serine hydroxymethyltransferase OS=Caenorhabditis briggsae GN=mel-32 PE=3 SV=3)

HSP 1 Score: 56.225 bits (134), Expect = 8.154e-16
Identity = 36/89 (40.45%), Postives = 52/89 (58.43%), Query Frame = 3 
Query:  504 AAGAIPSP*EHADVVTPTTHKSLRGPRGAMSLLWQGLLYIINRG-KTCYMIDN--NKGIKLAMQDLMWPTYPHMAGSTPVLRQATTSEY 761
            AAG IPSP E++DVVT TTHKSLRGPRGAM    +G+  +  +G +T Y ++   N  +   +Q    P    +AG    L+Q  + ++
Sbjct:  267 AAGLIPSPFEYSDVVTTTTHKSLRGPRGAMIFYRKGVRSVNAKGVETLYDLEEKINSAVFPGLQG--GPHNHTIAGIAVALKQCLSEDF 353

HSP 2 Score: 48.9062 bits (115), Expect = 8.154e-16
Identity = 21/49 (42.86%), Postives = 36/49 (73.47%), Query Frame = 2 
Query:  347 KMDKSATLFKP*LIDAGANAYARLANYERTRKVCNKLTAIMLADIAHIT 493
            K++++A LF+P ++ AG + YAR  +YER RK+  K  A +++D+AHI+
Sbjct:  215 KLEENAMLFRPKVLIAGVSCYARHLDYERFRKIATKAGAYLMSDMAHIS 263
The following BLAST results are available for this feature:
BLAST of EY650440 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 105
Match NameE-valueIdentityDescription
GLYM_FLAPR2.096e-2545.98Serine hydroxymethyltransferase 1, mitochondrial O... [more]
GLYM_SOLTU2.728e-2564.81Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_ARATH3.550e-2566.67Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYM_PEA1.018e-2445.98Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYN_FLAPR2.921e-2462.96Serine hydroxymethyltransferase 2, mitochondrial O... [more]
GLYC_ASHGO2.863e-1641.30Serine hydroxymethyltransferase, cytosolic OS=Ashb... [more]
GLYM_ASHGO3.717e-1641.30Serine hydroxymethyltransferase, mitochondrial OS=... [more]
GLYC_CANGA3.722e-1642.05Serine hydroxymethyltransferase, cytosolic OS=Cand... [more]
GLYC_YEAST6.287e-1643.18Serine hydroxymethyltransferase, cytosolic OS=Sacc... [more]
GLYC_CAEBR8.154e-1640.45Serine hydroxymethyltransferase OS=Caenorhabditis ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-011-A10-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY650440 ID=EY650440; Name=EY650440; organism=Citrus sinensis; type=EST; length=786bp
CTGTATGACATCCAAATTGCAGTATATGCTGCATATCGAATACAAAGCTG
ATGATTAAACATGAGCTCTCCATCAATATATGGGCGTTCAGATATAAAGG
CTTCAATTTCATTAACAATTGATTTTACTTCTTCATGCATACGAGAGATA
CGTTCATGTATCTAATATCACCAAAATGGATATTTAGGGTCATTATATAC
AAGTCTGGACAGGACGGGGAAGGTTTACGAACTACAATAAGACACGCATA
CAACTGAGGTGTCAGATCCAGATGGAATTGATGGGCAGACTATACCTGGC
AATATATATGATAGTAAAATGTGTTGTGACTTACATCATTGTGTTGAAGA
TGGATAAAAGCGCCACCCTCTTCAAGCCATAATTGATTGATGCTGGTGCT
AACGCTTATGCACGATTGGCCAATTATGAGCGTACTCGCAAGGTTTGCAA
CAAACTAACAGCTATAATGTTGGCAGATATAGCACACATTACTGAAGGCG
TGAGCTGCTGGCGCCATCCCTTCACCTTGAGAGCATGCAGATGTTGTGAC
GCCCACAACTCACAAATCACTTCGTGGGCCACGTGGAGCCATGAGTCTCT
TGTGGCAAGGACTGTTGTATATAATCAACAGAGGGAAGACGTGCTACATG
ATCGACAATAACAAAGGCATTAAGCTGGCTATGCAGGACTTGATGTGGCC
CACATATCCGCACATGGCTGGTTCTACACCCGTGCTGAGACAGGCCACTA
CTTCTGAGTATACGCCTCTGAACAGCTAATGCGCGC
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