EY650141

Overview
NameEY650141
Unique NameEY650141
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length878
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY650141_ssr110 microsatellite EY650141_ssr110:110..121. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_FLATR (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Flaveria trinervia GN=GDCSPA PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.371e-72
Identity = 96/132 (72.73%), Postives = 107/132 (81.06%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTIDIC TRADGFD+K   SDLKD DY S DVCGVLV YPGTEGE+LDY +FIKNAHANGV   MA+DLLALTI+ PPGELGADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGKP
Sbjct:  244 QTIDICKTRADGFDLKVVTSDLKDFDYSSGDVCGVLVQYPGTEGELLDYSEFIKNAHANGVKVVMASDLLALTILKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGKP 375

HSP 2 Score: 100.523 bits (249), Expect = 4.371e-72
Identity = 52/58 (89.66%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            AQGRL    NFQTMI DLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKK TFIIASN
Sbjct:  183 AQGRLESLLNFQTMITDLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKKTFIIASN 240
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_FLAAN (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Flaveria anomala GN=GDCSP PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.371e-72
Identity = 96/132 (72.73%), Postives = 107/132 (81.06%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTIDIC TRADGFD+K   SDLKD DY S DVCGVLV YPGTEGE+LDY +FIKNAHANGV   MA+DLLALTI+ PPGELGADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGKP
Sbjct:  244 QTIDICKTRADGFDLKVVTSDLKDFDYSSGDVCGVLVQYPGTEGELLDYSEFIKNAHANGVKVVMASDLLALTILKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGKP 375

HSP 2 Score: 100.523 bits (249), Expect = 4.371e-72
Identity = 52/58 (89.66%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            AQGRL    NFQTMI DLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKK TFIIASN
Sbjct:  183 AQGRLESLLNFQTMITDLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKKTFIIASN 240
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSPB_FLAPR (Glycine dehydrogenase [decarboxylating] B, mitochondrial OS=Flaveria pringlei GN=GDCSPB PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.371e-72
Identity = 96/132 (72.73%), Postives = 107/132 (81.06%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTIDIC TRADGFD+K   SDLKD DY S DVCGVLV YPGTEGE+LDY +FIKNAHANGV   MA+DLLALTI+ PPGELGADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGKP
Sbjct:  244 QTIDICKTRADGFDLKVVTSDLKDFDYSSGDVCGVLVQYPGTEGELLDYSEFIKNAHANGVKVVMASDLLALTILKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGKP 375

HSP 2 Score: 100.523 bits (249), Expect = 4.371e-72
Identity = 52/58 (89.66%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            AQGRL    NFQTMI DLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKK TFIIASN
Sbjct:  183 AQGRLESLLNFQTMITDLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKKTFIIASN 240
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSPA_FLAPR (Glycine dehydrogenase [decarboxylating] A, mitochondrial OS=Flaveria pringlei GN=GDCSPA PE=2 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 5.700e-72
Identity = 96/132 (72.73%), Postives = 107/132 (81.06%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTIDIC TRADGFD+K   SDLKD DY S DVCGVLV YPGTEGE+LDY +FIKNAHANGV   MA+DLLALTI+ PPGELGADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGKP
Sbjct:  247 QTIDICKTRADGFDLKVVTSDLKDFDYSSGDVCGVLVQYPGTEGELLDYSEFIKNAHANGVKVVMASDLLALTILKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGKP 378

HSP 2 Score: 100.138 bits (248), Expect = 5.700e-72
Identity = 51/58 (87.93%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            AQGRL    NFQTM+ DLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKK TFIIASN
Sbjct:  186 AQGRLESLLNFQTMVTDLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKKTFIIASN 243
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_SOLTU (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Solanum tuberosum GN=GDCSP PE=2 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 3.060e-70
Identity = 95/132 (71.97%), Postives = 109/132 (82.58%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTIDIC TRADGFD+K    DLKDIDYKS DVCGVLV YPGTEGE+LDYG+FIKNAHA+GV   MA+DLLALT++ PPGELGADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDS+GKP
Sbjct:  245 QTIDICKTRADGFDLKVVTVDLKDIDYKSGDVCGVLVQYPGTEGEILDYGEFIKNAHAHGVKVVMASDLLALTMLKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGLSVDSTGKP 376

HSP 2 Score: 94.7449 bits (234), Expect = 3.060e-70
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            +QGRL    N+QTMI DLTGLPMSNASLLDEGTAAAEAMAMCNNI KGKK TF+IASN
Sbjct:  184 SQGRLESLLNYQTMITDLTGLPMSNASLLDEGTAAAEAMAMCNNILKGKKKTFLIASN 241
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_PEA (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Pisum sativum GN=GDCSP PE=1 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 5.203e-70
Identity = 94/131 (71.76%), Postives = 106/131 (80.92%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGK 590
            QTIDIC TRADGF++K  V DLKDIDYKS DVCGVLV YPGTEGEVLDYG+FIK AHAN V   MA+DLLALT++ PPGE GADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGK
Sbjct:  267 QTIDICQTRADGFELKVVVKDLKDIDYKSGDVCGVLVQYPGTEGEVLDYGEFIKKAHANEVKVVMASDLLALTVLKPPGEFGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGK 397

HSP 2 Score: 98.2117 bits (243), Expect = 5.203e-70
Identity = 50/58 (86.21%), Postives = 52/58 (89.66%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            +QGRL    NFQTMI DLTGLPMSNASLLDEGTAAAEAM+MCNNIQKGKK TFIIASN
Sbjct:  206 SQGRLESLLNFQTMITDLTGLPMSNASLLDEGTAAAEAMSMCNNIQKGKKKTFIIASN 263
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP2_ARATH (Glycine dehydrogenase [decarboxylating] 2, mitochondrial OS=Arabidopsis thaliana GN=At4g33010 PE=1 SV=2)

HSP 1 Score: 191.43 bits (485), Expect = 6.785e-70
Identity = 94/131 (71.76%), Postives = 106/131 (80.92%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGK 590
            QTID+C TRADGFD+K   SDLKDIDY S DVCGVLV YPGTEGEVLDY +F+KNAHANGV   MATDLLALT++ PPGE GADI  GSA R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGK
Sbjct:  248 QTIDVCKTRADGFDLKVVTSDLKDIDYSSGDVCGVLVQYPGTEGEVLDYAEFVKNAHANGVKVVMATDLLALTVLKPPGEFGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGISVDSSGK 378

HSP 2 Score: 94.7449 bits (234), Expect = 6.785e-70
Identity = 48/58 (82.76%), Postives = 51/58 (87.93%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            +QGRL    NFQT+I DLTGLPMSNASLLDEGTAAAEAMAMCNNI KGKK TF+IASN
Sbjct:  187 SQGRLESLLNFQTVITDLTGLPMSNASLLDEGTAAAEAMAMCNNILKGKKKTFVIASN 244
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP1_ARATH (Glycine dehydrogenase [decarboxylating] 1, mitochondrial OS=Arabidopsis thaliana GN=GDCSP PE=1 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 1.259e-68
Identity = 91/131 (69.47%), Postives = 105/131 (80.15%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGK 590
            QTID+C TRADGFD+K    D+KD+DY S DVCGVLV YPGTEGEVLDYG+F+KNAHANGV   MATDLLALT++ PPGE GADI  GS  R GVP GYGG HAAF+ T+QEY+R +  R+ G +VDSSGK
Sbjct:  254 QTIDVCKTRADGFDLKVVTVDIKDVDYSSGDVCGVLVQYPGTEGEVLDYGEFVKNAHANGVKVVMATDLLALTMLKPPGEFGADIVVGSGQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGVSVDSSGK 384

HSP 2 Score: 93.5893 bits (231), Expect = 1.259e-68
Identity = 47/58 (81.03%), Postives = 51/58 (87.93%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            +QGRL    N+QT+I DLTGLPMSNASLLDEGTAAAEAMAMCNNI KGKK TF+IASN
Sbjct:  193 SQGRLESLLNYQTVITDLTGLPMSNASLLDEGTAAAEAMAMCNNILKGKKKTFVIASN 250
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_HAHCH (Glycine dehydrogenase [decarboxylating] OS=Hahella chejuensis (strain KCTC 2396) GN=gcvP PE=3 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.731e-39
Identity = 57/130 (43.85%), Postives = 83/130 (63.85%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSG 587
            QTI++   RA+   I+  V D +  D ++ D   VL+ YPG  G+V DY + IK AH +     MA D+L+LT++ PPGELGADI  G++ R GVP  +GG HAA++ T  EY+R +  R+ G +VD++G
Sbjct:  185 QTIEVVKGRAEPLGIEVVVGDPQK-DLQNHDYFAVLLQYPGVNGDVRDYRELIKTAHESNALAIMAADILSLTLLTPPGELGADIAIGNSQRFGVPLFFGGPHAAYIATKDEYKRSLPGRLVGVSVDANG 313

HSP 2 Score: 72.0182 bits (175), Expect = 3.731e-39
Identity = 37/59 (62.71%), Postives = 43/59 (72.88%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQ-KGKKNTFIIASN 186
            +QGRL    NFQTMI DLTG+ M+NASLLDEGTAAAEAM MC  +  K K N F +A +
Sbjct:  123 SQGRLEALLNFQTMIGDLTGMEMANASLLDEGTAAAEAMTMCRRVNGKNKSNVFFVAED 181
[Close]
BLAST of EY650141 vs. ExPASy Swiss-Prot
Match: GCSP_SYNY3 (Glycine dehydrogenase [decarboxylating] OS=Synechocystis sp. (strain PCC 6803) GN=gcvP PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.075e-38
Identity = 58/132 (43.94%), Postives = 79/132 (59.85%), Query Frame = 3 
Query:  198 QTIDICITRADGFDIKGGVSDLKDIDYKSCDVCGVLVHYPGTEGEVLDYGDFIKNAHANGVNGCMATDLLALTIVNPPGELGADIGWGSAHRCGVPRGYGGAHAAFVGTAQEYRRRIAWRMGGGNVDSSGKP 593
            QTI++  TRA+   I+  V D     + S  + G L+ YP T+G V DY  FI  AH +     +A D L+LT++ PPGELGADI  GS  R G+P GYGG HAA+  T  EY+R++  R+ G + D+ G P
Sbjct:  200 QTIEVIKTRANPLGIEVIVGDHHTFSF-STSIFGALLQYPATDGAVYDYRSFIDKAHQHQALVTLAADPLSLTLLTPPGELGADIAVGSTQRFGIPLGYGGPHAAYFATKAEYQRKMPGRIVGVSKDAHGNP 330

HSP 2 Score: 71.633 bits (174), Expect = 1.075e-38
Identity = 36/58 (62.07%), Postives = 43/58 (74.14%), Query Frame = 1 
Query:   13 AQGRLGGGGNFQTMIADLTGLPMSNASLLDEGTAAAEAMAMCNNIQKGKKNTFIIASN 186
            AQGRL    NFQTM+ DLTGL ++NASLLDEGTAAAEAMA+   + K K N F +A +
Sbjct:  139 AQGRLEALLNFQTMVMDLTGLEIANASLLDEGTAAAEAMALSYGVSKSKANAFFVAQD 196
The following BLAST results are available for this feature:
BLAST of EY650141 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 343
Match NameE-valueIdentityDescription
GCSP_FLATR4.371e-7272.73Glycine dehydrogenase [decarboxylating], mitochond... [more]
GCSP_FLAAN4.371e-7272.73Glycine dehydrogenase [decarboxylating], mitochond... [more]
GCSPB_FLAPR4.371e-7272.73Glycine dehydrogenase [decarboxylating] B, mitocho... [more]
GCSPA_FLAPR5.700e-7272.73Glycine dehydrogenase [decarboxylating] A, mitocho... [more]
GCSP_SOLTU3.060e-7071.97Glycine dehydrogenase [decarboxylating], mitochond... [more]
GCSP_PEA5.203e-7071.76Glycine dehydrogenase [decarboxylating], mitochond... [more]
GCSP2_ARATH6.785e-7071.76Glycine dehydrogenase [decarboxylating] 2, mitocho... [more]
GCSP1_ARATH1.259e-6869.47Glycine dehydrogenase [decarboxylating] 1, mitocho... [more]
GCSP_HAHCH3.731e-3943.85Glycine dehydrogenase [decarboxylating] OS=Hahella... [more]
GCSP_SYNY31.075e-3843.94Glycine dehydrogenase [decarboxylating] OS=Synecho... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-100-007-G02-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY650141 ID=EY650141; Name=EY650141; organism=Citrus sinensis; type=EST; length=878bp
CCCACGCGTCCGGCTCAAGGGAGACTGGGGGGGGGGGGGAATTTCCAAAC
CATGATTGCAGATCTTACTGGATTGCCTATGTCAAACGCCTCATTGCTTG
ATGAGGGAACTGCTGCTGCTGAGGCAATGGCTATGTGCAATAACATACAG
AAGGGAAAGAAGAACACTTTTATTATTGCTAGCAACTGGGGGGGCGGCAG
ACAATTGATATTTGTATTACTAGAGCTGATGGTTTTGATATTAAAGGTGG
TGTTTCGGATCTTAAGGATATTGATTACAAGTCATGTGATGTTTGTGGGG
TTTTGGTTCACTATCCAGGTACTGAGGGTGAAGTTTTGGATTATGGGGAT
TTCATTAAGAATGCTCATGCTAATGGGGTTAATGGGTGTATGGCAACGGA
TTTGTTGGCATTGACAATTGTGAATCCTCCAGGTGAACTGGGGGCTGATA
TTGGGTGGGGCTCTGCTCATAGGTGTGGTGTGCCAAGGGGGTATGGAGGT
GCTCATGCAGCATTTGTGGGTACCGCTCAAGAGTACAGAAGGAGGATCGC
ATGGAGGATGGGGGGGGGTAACGTGGATTCGTCAGGGAAACCACGCCGGG
GGTGGGGGAATTGCAAACAAGGAGGAAGCTTTCCCGGAGGGGCGCGGGCG
ACCCGCGACCTCTGGCGCCGGGGAGGGCGTACTGGCGAGCGGGGCGGGCG
TGGGTGGCGGGTTATGACCGGGGCCGGAGGGGCGTAAAGCCAGTCTTCAG
AGAGGGGACGTGGTTTATCGGTACTTTCGCCCCTGGGGGAAAAAACGTGG
TGAGCGAGGAACGGCGGGGCGTGTACGTCATTAGACTGTGGAAGGGGGGG
GGGGCGGAGGGGGAGCCAGTGGAGGGGC
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