EG358325

Overview
NameEG358325
Unique NameEG358325
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length540
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL2_PEA (Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=PAL2 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.042e-49
Identity = 94/122 (77.05%), Postives = 103/122 (84.43%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHAL N E  KN N SIF KI  FE+ELKTLLPKEVESAR A+E+GN  I N+IKECRSYPL +FVREELG   LTGEK ISPGEECDK+FTAICQGKIIDPLLECL +W+G+PLPI
Sbjct:  602 RQVLVDHALVNGESEKNLNTSIFQKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYKFVREELGTSLLTGEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPI 723
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PALY_MALDO (Phenylalanine ammonia-lyase (Fragment) OS=Malus domestica GN=PAL PE=2 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.484e-49
Identity = 93/122 (76.23%), Postives = 103/122 (84.43%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            R+VL++HALTN E  KNA+ SIF KIGAFEEELK LLPKEVESARSA E GN  +PNRI ECRSYPL +FVREELG  YLTGEK  SPGEECDKVF AICQGKIIDP+L CL+ W+G+PLPI
Sbjct:  113 REVLIEHALTNGESEKNASTSIFQKIGAFEEELKALLPKEVESARSAIEGGNAAVPNRIAECRSYPLYKFVREELGGEYLTGEKVRSPGEECDKVFQAICQGKIIDPILGCLEGWNGAPLPI 234
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL1_PRUAV (Phenylalanine ammonia-lyase 1 OS=Prunus avium GN=PAL1 PE=2 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 1.014e-48
Identity = 92/122 (75.41%), Postives = 107/122 (87.70%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLV+HALTN E+ KNA+ SIF KI AFEEELK LLPKEV+SAR+A +SG+  +PNRI ECRSYPL +FVREELGA YLTGEK  SPGEECDKVFTAIC+GKIIDP+L+CL+ W+G+PLPI
Sbjct:  595 RQVLVEHALTNGENEKNASTSIFQKIVAFEEELKVLLPKEVDSARAALDSGSAGVPNRITECRSYPLYKFVREELGAEYLTGEKVRSPGEECDKVFTAICEGKIIDPILDCLEGWNGAPLPI 716
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL1_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum GN=TPA1 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.324e-48
Identity = 92/122 (75.41%), Postives = 103/122 (84.43%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHA+ N E  KN N+SIF KIGAFE+ELK +LPKEVESAR+A ESGN  IPNRI ECRSYPL RFVR+ELG   LTGEK  SPGEECDKVFTA+C G+IIDP+LECLK W+G+PLPI
Sbjct:  593 RQVLVDHAMNNGESEKNVNSSIFQKIGAFEDELKAVLPKEVESARAALESGNPAIPNRITECRSYPLYRFVRKELGTELLTGEKVRSPGEECDKVFTAMCNGQIIDPMLECLKSWNGAPLPI 714
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL2_POPKI (Phenylalanine ammonia-lyase G2B OS=Populus kitakamiensis GN=PALG2B PE=3 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.729e-48
Identity = 94/122 (77.05%), Postives = 103/122 (84.43%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLV+HAL N E ++N+  SIF KIG+FEEELKTLLPKEVESAR   E+GN  IPNRIKECRSYPL +FVREELG   LTGEK  SPGEE DKVFTAIC GK+IDPLLECLKEWDG+PLPI
Sbjct:  588 RQVLVEHALVNGEKVRNSTTSIFQKIGSFEEELKTLLPKEVESARLEVENGNPAIPNRIKECRSYPLYKFVREELGTSLLTGEKVKSPGEEFDKVFTAICAGKLIDPLLECLKEWDGAPLPI 709
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PALY_MEDSA (Phenylalanine ammonia-lyase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 2.949e-48
Identity = 93/122 (76.23%), Postives = 102/122 (83.61%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHAL N E  KN N SIF KI  FEEELKTLLPKEVESAR+A+ESGN  IPN+I  CRSYPL +FVREELG   LTGE  ISPGEECDK+F+A+CQGKIIDPLLECL EW+G+PLPI
Sbjct:  603 RQVLVDHALVNGESEKNFNTSIFQKIATFEEELKTLLPKEVESARTAYESGNPTIPNKINGCRSYPLYKFVREELGTGLLTGENVISPGEECDKLFSAMCQGKIIDPLLECLGEWNGAPLPI 724
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL1_PEA (Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=PAL1 PE=2 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 3.852e-48
Identity = 91/122 (74.59%), Postives = 102/122 (83.61%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHAL N E  KN N SIF KI  FE+ELKTLLPKEVES R+A+ESGN  +PN+I  CRSYPL RFVR+ELG   LTGEK ISPGEECDK+FTAICQGKIIDPLL+CL +W+G+PLPI
Sbjct:  601 RQVLVDHALVNGESEKNLNTSIFQKIATFEDELKTLLPKEVESTRAAYESGNPTVPNKINGCRSYPLYRFVRQELGTGLLTGEKVISPGEECDKLFTAICQGKIIDPLLQCLGDWNGAPLPI 722
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL2_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum GN=PALA PE=2 SV=2)

HSP 1 Score: 189.889 bits (481), Expect = 6.570e-48
Identity = 95/122 (77.87%), Postives = 104/122 (85.25%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHAL N E+ KNAN+SIF KI AFE+ELK +LPKEVESAR+A ESGN  I NRIKECRSYPL RFVR ELGA  LTGEK  SPGEECDKVFTA+C G+IID LLECLKEW+G+PLPI
Sbjct:  590 RQVLVDHALQNGENEKNANSSIFQKILAFEDELKAVLPKEVESARAALESGNPAIANRIKECRSYPLYRFVRGELGAELLTGEKVRSPGEECDKVFTAMCNGQIIDSLLECLKEWNGAPLPI 711
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PAL2_ARATH (Phenylalanine ammonia-lyase 2 OS=Arabidopsis thaliana GN=PAL2 PE=1 SV=2)

HSP 1 Score: 189.889 bits (481), Expect = 6.570e-48
Identity = 89/122 (72.95%), Postives = 106/122 (86.89%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQV+VDHAL+N E  KNA  SIF KIGAFEEELK +LPKEVE+AR+A+ +G   IPNRIKECRSYPL RFVREELG + LTGEK +SPGEE DKVFTA+C+GK+IDPL++CLKEW+G+P+PI
Sbjct:  595 RQVIVDHALSNGETEKNAVTSIFQKIGAFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEEFDKVFTAMCEGKLIDPLMDCLKEWNGAPIPI 716
[Close]
BLAST of EG358325 vs. ExPASy Swiss-Prot
Match: PALY_TRISU (Phenylalanine ammonia-lyase OS=Trifolium subterraneum GN=PAL1 PE=3 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 8.580e-48
Identity = 91/122 (74.59%), Postives = 103/122 (84.43%), Query Frame = -2 
Query:  174 RQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLNRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 539
            RQVLVDHAL N E  KN+N SIF KI  FEEELKTLLPKEVESAR+A+E+GN  I N+I  CRSYPL +FVREELG   LTGE+ ISPGEECDK+FTA+CQGKIIDPLL+CL EW+G+PLPI
Sbjct:  603 RQVLVDHALVNGESEKNSNTSIFQKIATFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSLLTGERVISPGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPI 724
The following BLAST results are available for this feature:
BLAST of EG358325 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
PAL2_PEA2.042e-4977.05Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=... [more]
PALY_MALDO3.484e-4976.23Phenylalanine ammonia-lyase (Fragment) OS=Malus do... [more]
PAL1_PRUAV1.014e-4875.41Phenylalanine ammonia-lyase 1 OS=Prunus avium GN=P... [more]
PAL1_TOBAC1.324e-4875.41Phenylalanine ammonia-lyase OS=Nicotiana tabacum G... [more]
PAL2_POPKI1.729e-4877.05Phenylalanine ammonia-lyase G2B OS=Populus kitakam... [more]
PALY_MEDSA2.949e-4876.23Phenylalanine ammonia-lyase OS=Medicago sativa PE=... [more]
PAL1_PEA3.852e-4874.59Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=... [more]
PAL2_TOBAC6.570e-4877.87Phenylalanine ammonia-lyase OS=Nicotiana tabacum G... [more]
PAL2_ARATH6.570e-4872.95Phenylalanine ammonia-lyase 2 OS=Arabidopsis thali... [more]
PALY_TRISU8.580e-4874.59Phenylalanine ammonia-lyase OS=Trifolium subterran... [more]

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Properties
Property NameValue
Genbank description13a12 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl013a12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358325 ID=EG358325; Name=EG358325; organism=Citrus sinensis; type=EST; length=540bp
ACATAAATTTCACCAAAATTACAGACTTTAAGAAGGGGGAAAAATAGAAT
TAAGAACACAAAGTGAAAGCATAGAGACAGTATCATCCTCACAGTAGAAG
AATTATATTTGTTAACCTTCCAAGCGACATTAAAATATATATAAAAAAAA
AGGCATAAAAGAAAGCATTAACAAATTGGAAGAGGACTCCCATCCCATTC
TTTAAGACATTCAAGCAGTGGATCAATTATTTTTCCCTGACAAATCGCTG
TAAACACCTTATCACATTCCTCGCCTGGTGATATCGCCTTCTCTCCTGTC
AGATACCGAGCCCCCAACTCTTCCCTTACAAACCTATTCAATGGGTAGGA
CCTGCACTCCTTAATCCTGTTTGGTATTTCCAAATTACCACTCTCAAAAG
CACTTCTAGCACTCTCAACTTCTTTCGGCAAAAGGGTCTTCAACTCCTCC
TCGAAAGCACCTATCTTTAAGAAGATTGAAGCATTTGCATTCTTCAAATC
TTCATTGTTTGTCAATGCATGATCAACAAGGACTTGTCTC
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