EY702546

Overview
NameEY702546
Unique NameEY702546
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length875
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF1_PETHY (Actin-depolymerizing factor 1 OS=Petunia hybrida GN=ADF1 PE=2 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 1.072e-59
Identity = 103/120 (85.83%), Postives = 113/120 (94.17%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRT+RFIV+KIEEKQKQV+VEK+GEPT++YEDFAASLP  ECRYAVYD+DFVTAENCQKSRIFF AW PDTARVRSKMIYASSKDRFKRELDGIQVELQA DP+EMGLD+ + RAN
Sbjct:   20 ELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASLPENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVELQACDPTEMGLDVIQSRAN 139

HSP 2 Score: 36.965 bits (84), Expect = 1.072e-59
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKL 116
            MANAASGMAVHDDCKL
Sbjct:    1 MANAASGMAVHDDCKL 16
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF2_PETHY (Actin-depolymerizing factor 2 OS=Petunia hybrida GN=ADF2 PE=2 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 8.937e-59
Identity = 101/123 (82.11%), Postives = 115/123 (93.50%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN*IK 495
            ELKAKRTYRFI++KIEEKQK+V+VEKLGEPT++YEDF A LPA+ECRYAVYD+DF+T EN QKSRIFF AW+PDTARVRSKMIYASSKDRFKRELDGIQVELQATDP+EMGLD+F+ RA  I+
Sbjct:   20 ELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGLPADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPTEMGLDVFRSRAGGIE 142

HSP 2 Score: 37.3502 bits (85), Expect = 8.937e-59
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            MANAASGMAVHDDCKL+
Sbjct:    1 MANAASGMAVHDDCKLK 17
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF4_ARATH (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana GN=ADF4 PE=2 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 2.587e-58
Identity = 101/120 (84.17%), Postives = 111/120 (92.50%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRT+RFIV+KIEEKQKQVIVEK+GEP  TYEDFAASLPA+ECRYA+YD+DFVTAENCQKS+IFF AW PD A+VRSKMIYASSKDRFKRELDGIQVELQATDP+EM LD+ K R N
Sbjct:   20 ELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVLKSRVN 139

HSP 2 Score: 36.965 bits (84), Expect = 2.587e-58
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKL 116
            MANAASGMAVHDDCKL
Sbjct:    1 MANAASGMAVHDDCKL 16
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF1_ARATH (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana GN=ADF1 PE=1 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 2.587e-58
Identity = 99/120 (82.50%), Postives = 113/120 (94.17%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRT+RFIV+KIEEKQKQV+VEK+G+P QTYE+FAA LPA+ECRYA+YD+DFVTAENCQKS+IFF AW PD A+VRSKMIYASSKDRFKRELDGIQVELQATDP+EM LD+F+ RAN
Sbjct:   20 ELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSRAN 139

HSP 2 Score: 36.965 bits (84), Expect = 2.587e-58
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKL 116
            MANAASGMAVHDDCKL
Sbjct:    1 MANAASGMAVHDDCKL 16
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF2_ARATH (Actin-depolymerizing factor 2 OS=Arabidopsis thaliana GN=ADF2 PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 6.791e-56
Identity = 97/120 (80.83%), Postives = 111/120 (92.50%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRT+R IV+KIE+KQ  VIVEKLGEP Q+Y+DFAASLPA++CRY +YD+DFVTAENCQKS+IFF AW+PDTA+VR KMIYASSKDRFKRELDGIQVELQATDP+EMGLD+FK R N
Sbjct:   20 ELKAKRTFRTIVYKIEDKQ--VIVEKLGEPEQSYDDFAASLPADDCRYCIYDFDFVTAENCQKSKIFFIAWSPDTAKVRDKMIYASSKDRFKRELDGIQVELQATDPTEMGLDVFKSRTN 137

HSP 2 Score: 37.3502 bits (85), Expect = 6.791e-56
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            MANAASGMAVHDDCKL+
Sbjct:    1 MANAASGMAVHDDCKLK 17
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF7_ORYSJ (Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica GN=ADF7 PE=3 SV=2)

HSP 1 Score: 205.297 bits (521), Expect = 2.558e-55
Identity = 95/120 (79.17%), Postives = 111/120 (92.50%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRTYRFI++KI+EK+K V+VEK+GEP   Y+DFAASLPA ECRYA++DYDFVT ENCQKS+IFF AW+PDT+RVRSKMIYASSKDRFKRELDGIQVELQATDP+E+GLD+ + RAN
Sbjct:   20 ELKAKRTYRFIIYKIDEKKKMVVVEKVGEPVLNYDDFAASLPANECRYAIFDYDFVTEENCQKSKIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVELQATDPTEVGLDVIRGRAN 139

HSP 2 Score: 32.3426 bits (72), Expect = 2.558e-55
Identity = 14/17 (82.35%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            MANAASGMAV D+CKL+
Sbjct:    1 MANAASGMAVDDECKLK 17
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF3_ARATH (Actin-depolymerizing factor 3 OS=Arabidopsis thaliana GN=ADF3 PE=1 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 5.668e-55
Identity = 95/120 (79.17%), Postives = 110/120 (91.67%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELK KRT+RFI++KIEE QKQVIVEK+GEP QT+ED AASLPA+ECRYA++D+DFV++E   +SRIFF AW+PDTARVRSKMIYASSKDRFKRELDGIQVELQATDP+EM LD+FK RAN
Sbjct:   20 ELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASLPADECRYAIFDFDFVSSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRAN 139

HSP 2 Score: 37.3502 bits (85), Expect = 5.668e-55
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            MANAASGMAVHDDCKL+
Sbjct:    1 MANAASGMAVHDDCKLK 17
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF10_ARATH (Actin-depolymerizing factor 10 OS=Arabidopsis thaliana GN=ADF10 PE=2 SV=1)

HSP 1 Score: 200.29 bits (508), Expect = 6.697e-53
Identity = 93/120 (77.50%), Postives = 106/120 (88.33%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKR YRFIVFKI+EK +QV+++KLG P +TYEDF  S+P +ECRYAVYDYDF T ENCQKS+IFF AW+PDT+RVRSKM+YASSKDRFKRELDGIQVELQATDPSEM LD+ K R N
Sbjct:   20 ELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSLDIIKGRVN 139

HSP 2 Score: 29.261 bits (64), Expect = 6.697e-53
Identity = 13/26 (50.00%), Postives = 20/26 (76.92%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLEGGGVESQEN 146
            MAN+ASGM V D+CKL+   ++++ N
Sbjct:    1 MANSASGMHVSDECKLKFLELKAKRN 26
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF8_ARATH (Actin-depolymerizing factor 8 OS=Arabidopsis thaliana GN=ADF8 PE=2 SV=2)

HSP 1 Score: 195.667 bits (496), Expect = 2.102e-51
Identity = 90/120 (75.00%), Postives = 107/120 (89.17%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRAN 486
            ELKAKRTYRFIVFKI+EK +QV +EKLG P +TY+DF +S+P +ECRYAVYD+DF T +NCQKS+IFF AW+PDT+RVRSKM+YASSKDRFKRE++GIQVELQATDPSEM LD+ K R N
Sbjct:   20 ELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPDDECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQATDPSEMSLDIIKGRLN 139

HSP 2 Score: 28.8758 bits (63), Expect = 2.102e-51
Identity = 11/17 (64.71%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            MAN+ASGM V+D+CK++
Sbjct:    1 MANSASGMHVNDECKIK 17
[Close]
BLAST of EY702546 vs. ExPASy Swiss-Prot
Match: ADF1_ORYSJ (Actin-depolymerizing factor 1 OS=Oryza sativa subsp. japonica GN=ADF1 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.743e-51
Identity = 90/119 (75.63%), Postives = 109/119 (91.60%), Query Frame = 1 
Query:  127 ELKAKRTYRFIVFKIEEKQKQVIVEKLGEPTQTYEDFAASLPAEECRYAVYDYDFVTAENCQKSRIFFSAWAPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPSEMGLDLFKDRA 483
            ELKAKR++RFIVFKI EK +QV+V++LG+P ++Y+DF A LPA+ECRYAV+D+DFVT ENCQKS+IFF +WAPDT+RVRSKM+YASSKDRFKRELDGIQVELQATDPSEM +D+ K RA
Sbjct:   20 ELKAKRSFRFIVFKINEKVQQVVVDRLGQPGESYDDFTACLPADECRYAVFDFDFVTDENCQKSKIFFISWAPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSMDIVKSRA 138

HSP 2 Score: 29.261 bits (64), Expect = 2.743e-51
Identity = 12/17 (70.59%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:   69 MANAASGMAVHDDCKLE 119
            M+N+ASGMAV D+CKL+
Sbjct:    1 MSNSASGMAVCDECKLK 17
The following BLAST results are available for this feature:
BLAST of EY702546 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 72
Match NameE-valueIdentityDescription
ADF1_PETHY1.072e-5985.83Actin-depolymerizing factor 1 OS=Petunia hybrida G... [more]
ADF2_PETHY8.937e-5982.11Actin-depolymerizing factor 2 OS=Petunia hybrida G... [more]
ADF4_ARATH2.587e-5884.17Actin-depolymerizing factor 4 OS=Arabidopsis thali... [more]
ADF1_ARATH2.587e-5882.50Actin-depolymerizing factor 1 OS=Arabidopsis thali... [more]
ADF2_ARATH6.791e-5680.83Actin-depolymerizing factor 2 OS=Arabidopsis thali... [more]
ADF7_ORYSJ2.558e-5579.17Actin-depolymerizing factor 7 OS=Oryza sativa subs... [more]
ADF3_ARATH5.668e-5579.17Actin-depolymerizing factor 3 OS=Arabidopsis thali... [more]
ADF10_ARATH6.697e-5377.50Actin-depolymerizing factor 10 OS=Arabidopsis thal... [more]
ADF8_ARATH2.102e-5175.00Actin-depolymerizing factor 8 OS=Arabidopsis thali... [more]
ADF1_ORYSJ2.743e-5175.63Actin-depolymerizing factor 1 OS=Oryza sativa subs... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-009-C09-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702546 ID=EY702546; Name=EY702546; organism=Citrus sinensis; type=EST; length=875bp
CAACAAACTTTCCTTCACCTTGCAAAATTTCTCTGTACTTTGCTTCTTAG
ATCTTGATCCTAGAAACCATGGCTAATGCTGCTTCTGGGATGGCTGTACA
TGATGACTGTAAGCTAGAGGGCGGGGGAGTTGAAAGCCAAGAGAACCTAC
CGCTTCATAGTATTTAAGATTGAGGAGAAGCAGAAGCAGGTCATTGTGGA
AAAACTTGGTGAGCCAACTCAAACCTATGAAGATTTTGCTGCCAGCCTTC
CTGCTGAGGAGTGCCGATATGCTGTGTATGATTATGATTTTGTTACCGCA
GAGAACTGCCAAAAAAGCAGGATTTTCTTCAGTGCATGGGCTCCCGACAC
TGCAAGAGTAAGAAGCAAGATGATTTATGCAAGCTCCAAGGACAGGTTCA
AGAGAGAGCTAGATGGCATTCAAGTGGAGCTGCAGGCAACTGATCCATCT
GAGATGGGTCTTGACCTATTTAAAGACCGTGCCAACTAAATAAAGGCTCC
TGATCTCAGCAATTGAGATTGGTTTTATCTTTTGTGCTTTACCTAAAGGT
GAAATGTTAATAGTTTGTGGCGGCAGCTTGAATCTAACTGAATTATGATA
TTGGTTTGGTGTTCAAGTTTCCCCGATGCAGTATTCTCCTCTAGAACCAT
TTCAGGGGCCTTTATGATGGCTCTATTGATTCTGCCGGGGCGTGCAACTT
ATCCGATGAGCGGCTACTTATACCCATTCCCCCAGCTCACGCGTTTCAAC
TGATGGGGGATGGAATACCCTCCTGGTAAGGGTGTTTTTACCTTAAAAAT
GGAGGTTCACTTTTGAACTTTATAAAGGAGGCTTCATTCTCCTCGCTAAA
AAAAAAAAAAAAAAAAGGCGCCCCC
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