EY702525

Overview
NameEY702525
Unique NameEY702525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length837
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12221 contig Csv1_Contig12221:18..850. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_WHEAT (Elongation factor 1-alpha OS=Triticum aestivum GN=TEF1 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 5.199e-89
Identity = 125/142 (88.03%), Postives = 127/142 (89.44%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEKTHINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 100.908 bits (250), Expect = 5.199e-89
Identity = 48/56 (85.71%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+KVPFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYEEIVKEVSSYLKKVGYNPDKVPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_ORYSJ (Elongation factor 1-alpha OS=Oryza sativa subsp. japonica GN=REFA1 PE=2 SV=2)

HSP 1 Score: 249.21 bits (635), Expect = 8.847e-89
Identity = 125/142 (88.03%), Postives = 127/142 (89.44%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEKTHINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 100.138 bits (248), Expect = 8.847e-89
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_MANES (Elongation factor 1-alpha OS=Manihot esculenta GN=EF1 PE=3 SV=1)

HSP 1 Score: 247.284 bits (630), Expect = 3.342e-88
Identity = 124/142 (87.32%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 100.138 bits (248), Expect = 3.342e-88
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_TOBAC (Elongation factor 1-alpha OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 5.687e-88
Identity = 124/142 (87.32%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 100.138 bits (248), Expect = 5.687e-88
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_ARATH (Elongation factor 1-alpha OS=Arabidopsis thaliana GN=A1 PE=1 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 7.418e-88
Identity = 124/142 (87.32%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 99.7525 bits (247), Expect = 7.418e-88
Identity = 46/56 (82.14%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EI+KEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_MAIZE (Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 9.676e-88
Identity = 125/142 (88.03%), Postives = 127/142 (89.44%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEKTHINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 96.6709 bits (239), Expect = 9.676e-88
Identity = 46/56 (82.14%), Postives = 49/56 (87.50%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+ FVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIHFVPISGFEG 198

HSP 3 Score: 67.781 bits (164), Expect = 8.764e-11
Identity = 36/70 (51.43%), Postives = 44/70 (62.86%), Query Frame = 1 
Query:  622 RRVDTTPRKSHLCPSLGSEGDNMIERSTNLDWDQGPTLLDALAILMKRKGPRQSP-SSALQGRNNMGGIG 828
            ++V   P K H  P  G EGDNMIERSTNLDW +GPTLL+AL ++ + K P   P    LQ    +GGIG
Sbjct:  179 KKVGYNPDKIHFVPISGFEGDNMIERSTNLDWYKGPTLLEALDLINEPKRPSDKPLRLPLQDVYKIGGIG 248
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_SOLLC (Elongation factor 1-alpha OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 1.647e-87
Identity = 123/142 (86.62%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HI+IVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKIHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 100.138 bits (248), Expect = 1.647e-87
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_VICFA (Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 245.743 bits (626), Expect = 2.148e-87
Identity = 123/142 (86.62%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFET++YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETSKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 98.9821 bits (245), Expect = 2.148e-87
Identity = 46/56 (82.14%), Postives = 49/56 (87.50%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSK RY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKGRYEEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A_SOYBN (Elongation factor 1-alpha OS=Glycine max GN=TEFS1 PE=3 SV=2)

HSP 1 Score: 244.202 bits (622), Expect = 2.802e-87
Identity = 122/142 (85.92%), Postives = 126/142 (88.73%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HI+IVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETT+YYC  IDAPGHRDFIKNMITGTSQADCAVLIIDST+GGFEAGISKDGQTREHALL+FT
Sbjct:    1 MGKEKVHISIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLSFT 142

HSP 2 Score: 100.138 bits (248), Expect = 2.802e-87
Identity = 47/56 (83.93%), Postives = 50/56 (89.29%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+P+K+PFVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEG 198
[Close]
BLAST of EY702525 vs. ExPASy Swiss-Prot
Match: EF1A1_DAUCA (Elongation factor 1-alpha OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.801e-86
Identity = 122/142 (85.92%), Postives = 124/142 (87.32%), Query Frame = 2 
Query:   86 MGKEKTHINIVVIXXXXXXXXXXXXXLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTRYYCAGIDAPGHRDFIKNMITGTSQADCAVLIIDSTSGGFEAGISKDGQTREHALLAFT 511
            MGKEK HINIVVI             LIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFET +YYC  IDAPGHRDFIKNMITGTSQADCAVLIID T+GGFEAGISKDGQTREHALLAFT
Sbjct:    1 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETNKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDPTTGGFEAGISKDGQTREHALLAFT 142

HSP 2 Score: 98.2117 bits (243), Expect = 1.801e-86
Identity = 47/56 (83.93%), Postives = 49/56 (87.50%), Query Frame = 3 
Query:  513 LGVKQMICCWNKMDAPTPKYSKARYHEIVKEVSSYLKKGGYHPEKVPFVPISRFRG 680
            LGVKQMICC NKMDA TPKYSKARY EIVKEVSSYLKK GY+PEK+ FVPIS F G
Sbjct:  143 LGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKIAFVPISGFEG 198
The following BLAST results are available for this feature:
BLAST of EY702525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A_WHEAT5.199e-8988.03Elongation factor 1-alpha OS=Triticum aestivum GN=... [more]
EF1A_ORYSJ8.847e-8988.03Elongation factor 1-alpha OS=Oryza sativa subsp. j... [more]
EF1A_MANES3.342e-8887.32Elongation factor 1-alpha OS=Manihot esculenta GN=... [more]
EF1A_TOBAC5.687e-8887.32Elongation factor 1-alpha OS=Nicotiana tabacum PE=... [more]
EF1A_ARATH7.418e-8887.32Elongation factor 1-alpha OS=Arabidopsis thaliana ... [more]
EF1A_MAIZE9.676e-8888.03Elongation factor 1-alpha OS=Zea mays GN=EF1A PE=3... [more]
EF1A_SOLLC1.647e-8786.62Elongation factor 1-alpha OS=Solanum lycopersicum ... [more]
EF1A_VICFA2.148e-8786.62Elongation factor 1-alpha OS=Vicia faba PE=2 SV=1[more]
EF1A_SOYBN2.802e-8785.92Elongation factor 1-alpha OS=Glycine max GN=TEFS1 ... [more]
EF1A1_DAUCA1.801e-8685.92Elongation factor 1-alpha OS=Daucus carota PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-009-A08-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702525 ID=EY702525; Name=EY702525; organism=Citrus sinensis; type=EST; length=837bp
CCCTCGCGTCCGCAGCTTCTGCTAGTCGGCGAGAGAGAAACCTTTTAGCA
AATTTCATATCAAGGGTTTATACTAGATTTGCATAATGGGTAAGGAGAAG
ACTCACATCAACATTGTGGTCATCGGCCATGGCGGCTCTGGAAAGTCGAC
CACCACCGGACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGA
TCGAGAGGTTTGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAA
TACGCCTGGGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTAC
CATTGATATTGCCTTGTGGAAGTTCGAGACCACCAGGTACTACTGCGCCG
GCATTGATGCTCCTGGACATCGTGACTTTATCAAGAACATGATTACCGGT
ACCTCACAGGCTGACTGTGCCGTCCTCATCATTGACTCCACCAGCGGTGG
TTTTGAAGCTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGC
TTGCTTTCACCCCTTGGTGTGAAGCAGATGATTTGCTGCTGGAACAAGAT
GGACGCCCCCACCCCTAAGTACTCAAAGGCTAGGTACCATGAAATTGTGA
AGGAAGTCTCTTCCTACCTGAAGAAGGGTGGATACCACCCCGAGAAAGTC
CCATTTGTGCCCATCTCTCGGTTCCGAGGGTGACAACATGATTGAGAGGT
CTACCAACCTTGACTGGGACCAGGGTCCAACCCTGCTGGATGCTCTTGCA
ATACTCATGAAGCGAAAGGGCCCTCGACAAAGCCCTTCGTCTGCACTTCA
GGGACGGAACAACATGGGGGGAATCGGACTGCCCAGC
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