DY257649

Overview
NameDY257649
Unique NameDY257649
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1166
Libraries
Library NameType
AbsCFruitcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DY257649_ssr1042 microsatellite DY257649_ssr1042:1042..1053. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_ARATH (26S proteasome non-ATPase regulatory subunit 14 OS=Arabidopsis thaliana GN=RPN11 PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 8.716e-61
Identity = 116/159 (72.96%), Postives = 126/159 (79.25%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLD 480
            ++T QSFEALNQRAVAVVVDPI SVK  VVIDAFR INPQT+ML Q PR TTSNLGHLNKPSIQ+ IHG    YYSIAINYRK           HK KWTDGLTL RFD HS+TNEQTVQ ML+LA KYNKAVQEE +L P+KLAI NVG QD+KKHL+
Sbjct:  126 INTQQSFEALNQRAVAVVVDPIQSVKGKVVIDAFRSINPQTIMLGQEPRQTTSNLGHLNKPSIQALIHGLNRHYYSIAINYRKNELEEKMLLNLHKKKWTDGLTLRRFDTHSKTNEQTVQEMLSLAAKYNKAVQEEDELSPEKLAIVNVGRQDAKKHLE 284

HSP 2 Score: 30.0314 bits (66), Expect = 8.716e-61
Identity = 13/22 (59.09%), Postives = 17/22 (77.27%), Query Frame = 2 
Query:  488 VSYFISSNIFLTFGTMLDSIIF 553
            VS  +SSNI  T GTMLD+++F
Sbjct:  287 VSNLMSSNIVQTLGTMLDTVVF 308
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_DROME (26S proteasome non-ATPase regulatory subunit 14 OS=Drosophila melanogaster GN=Rpn11 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.375e-45
Identity = 92/159 (57.86%), Postives = 114/159 (71.70%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLD 480
            ++T QSFEAL++RAVAVVVDPI SVK  VVIDAFRLINP  ++L Q PR TTSNLGHL KPS+Q+ IHG    YYSI+INYRK           HK  W DGLTL  ++ H   NE TV  ML+LA  YNK++++E ++ P++LAI NVG QD K+HL+
Sbjct:  125 INTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINPNMLVLGQEPRQTTSNLGHLQKPSVQALIHGLNRHYYSISINYRKNELEQKMLLNLHKKSWKDGLTLSDYNEHCSINEDTVAEMLDLAKNYNKSLEDEEKMTPEQLAIKNVGKQDPKRHLE 283

HSP 2 Score: 21.557 bits (44), Expect = 1.375e-45
Identity = 9/22 (40.91%), Postives = 13/22 (59.09%), Query Frame = 2 
Query:  488 VSYFISSNIFLTFGTMLDSIIF 553
            V   + +NI    G MLD+I+F
Sbjct:  286 VDKVMQNNIVQCLGAMLDTIVF 307
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_MOUSE (26S proteasome non-ATPase regulatory subunit 14 OS=Mus musculus GN=Psmd14 PE=1 SV=2)

HSP 1 Score: 180.259 bits (456), Expect = 5.168e-45
Identity = 94/159 (59.12%), Postives = 111/159 (69.81%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLD 480
            ++T QSFEAL++RAVAVVVDPI SVK  VVIDAFRLIN   M+L   PR TTSNLGHLNKPSIQ+ IHG    YYSI INYRK           HK  W +GLTL  +  H + NE  V+ ML LA  YNKAV+EE ++ P++LAI NVG QD K+HL+
Sbjct:  127 INTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLE 285

HSP 2 Score: 21.1718 bits (43), Expect = 5.168e-45
Identity = 8/22 (36.36%), Postives = 13/22 (59.09%), Query Frame = 2 
Query:  488 VSYFISSNIFLTFGTMLDSIIF 553
            V   ++SNI      MLD+++F
Sbjct:  288 VDVLMTSNIVQCLAAMLDTVVF 309
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_HUMAN (26S proteasome non-ATPase regulatory subunit 14 OS=Homo sapiens GN=PSMD14 PE=1 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 5.168e-45
Identity = 94/159 (59.12%), Postives = 111/159 (69.81%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLD 480
            ++T QSFEAL++RAVAVVVDPI SVK  VVIDAFRLIN   M+L   PR TTSNLGHLNKPSIQ+ IHG    YYSI INYRK           HK  W +GLTL  +  H + NE  V+ ML LA  YNKAV+EE ++ P++LAI NVG QD K+HL+
Sbjct:  127 INTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLE 285

HSP 2 Score: 21.1718 bits (43), Expect = 5.168e-45
Identity = 8/22 (36.36%), Postives = 13/22 (59.09%), Query Frame = 2 
Query:  488 VSYFISSNIFLTFGTMLDSIIF 553
            V   ++SNI      MLD+++F
Sbjct:  288 VDVLMTSNIVQCLAAMLDTVVF 309
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: RPN11_SCHPO (26S proteasome regulatory subunit rpn11 OS=Schizosaccharomyces pombe GN=rpn11 PE=1 SV=2)

HSP 1 Score: 165.236 bits (417), Expect = 6.504e-40
Identity = 85/159 (53.46%), Postives = 104/159 (65.41%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLD 480
            ++T QSFE L  RAVAVVVDPI SVK  VVIDAFRLINP T+M+ Q PR TTSNLGH+NKPSIQ+ IHG    YYS+ INY+K           HK  W  GL L  F+  ++ N  ++  M +L+ +Y + VQ E  L P++L I  VG QD KKHLD
Sbjct:  126 INTQQSFEQLTPRAVAVVVDPIQSVKGKVVIDAFRLINPSTLMMGQEPRQTTSNLGHINKPSIQALIHGLGRHYYSLRINYKKTELEEIMLLNLHKQPWAHGLLLENFNSAAEKNHASIDKMKSLSEQYTERVQNEVTLSPEQLRIQYVGKQDPKKHLD 284
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_CAEEL (26S proteasome non-ATPase regulatory subunit 14 OS=Caenorhabditis elegans GN=rpn-11 PE=1 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 5.154e-37
Identity = 81/158 (51.27%), Postives = 104/158 (65.82%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHL 477
            ++T QSFEAL+ RAVAVVVDPI SVK  VVIDAFR INPQ+M L Q PR TTSNLGHL KPSIQ+ IHG    YYSI I YR            +K+ W D +++  +    + N++ ++ ML LA  Y KA+++E  +  Q+LAI NVG  D K+H+
Sbjct:  129 INTQQSFEALSDRAVAVVVDPIQSVKGKVVIDAFRTINPQSMALNQEPRQTTSNLGHLQKPSIQALIHGLNRHYYSIPIAYRTHDLEQKMLLNLNKLSWMDAVSVENYSKCGEQNKEHLKAMLKLAKNYKKALEDEKNMTDQELAIKNVGKMDPKRHI 286
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: PSDE_DICDI (26S proteasome non-ATPase regulatory subunit 14 OS=Dictyostelium discoideum GN=psmD14 PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 1.658e-35
Identity = 78/158 (49.37%), Postives = 100/158 (63.29%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHL 477
            ++T QSFE L  RAVAVVVDP+ SV+  VVIDAFR I          PR  TSNLGHL  PSIQ+ IHG    YYSIAINYRK           HK KWT+GL + +FD H Q+NE+ +  +L L  +Y K++Q+E ++ P+K  ++ VG  D K+HL
Sbjct:  127 VNTQQSFEQLQSRAVAVVVDPLQSVRGKVVIDAFRTIKTSPTAE---PRQITSNLGHLQDPSIQALIHGLNRNYYSIAINYRKNELEQKMLLNLHKKKWTEGLIVDKFDTHEQSNEKQINNLLELTKQYQKSIQDEDKIEPEKKEVSAVGKLDPKRHL 281
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: RPN11_ASHGO (26S proteasome regulatory subunit RPN11 OS=Ashbya gossypii GN=RPN11 PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 4.084e-34
Identity = 78/161 (48.45%), Postives = 100/161 (62.11%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLDVT 486
            +DT +SFE LN RAVAVVVDPI SVK  VVIDAFRLI+P T++  Q PR TTSN+G LNKP+IQS IHG    YYS+ I+Y K           HK +W  GL +H +    + N +  +  + +A +Y K ++EE +L   +L    VG QD KKHL  T
Sbjct:  128 VDTQRSFEQLNSRAVAVVVDPIQSVKGKVVIDAFRLISPATVVRNQEPRQTTSNVGLLNKPNIQSLIHGLNRHYYSLNIDYHKTSAELNMLMNLHKEQWQSGLKMHDYKEKERINLEATKKSVRIAEQYTKRIEEEKELTEDELKTRYVGKQDPKKHLSET 288
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: RPN11_YEAST (26S proteasome regulatory subunit RPN11 OS=Saccharomyces cerevisiae GN=RPN11 PE=1 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.739e-30
Identity = 72/161 (44.72%), Postives = 99/161 (61.49%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLDVT 486
            ++T +SFE LN RAVAVVVDPI SVK  VVIDAFRLI+   ++    PR TTSN G LNK +IQ+ IHG    YYS+ I+Y K           HK +W  GL ++ ++   ++N    + M+ +A +Y+K ++EE +L  ++L    VG QD KKHL  T
Sbjct:  123 VNTQKSFEQLNSRAVAVVVDPIQSVKGKVVIDAFRLIDTGALINNLEPRQTTSNTGLLNKANIQALIHGLNRHYYSLNIDYHKTAKETKMLMNLHKEQWQSGLKMYDYEEKEESNLAATKSMVKIAEQYSKRIEEEKELTEEELKTRYVGRQDPKKHLSET 283
[Close]
BLAST of DY257649 vs. ExPASy Swiss-Prot
Match: RPN11_CANGA (26S proteasome regulatory subunit RPN11 OS=Candida glabrata GN=RPN11 PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 1.775e-29
Identity = 72/161 (44.72%), Postives = 97/161 (60.25%), Query Frame = 1 
Query:    4 LDTHQSFEALNQRAVAVVVDPIHSVKSNVVIDAFRLINPQTMMLVQ*PRHTTSNLGHLNKPSIQSFIHGFFILYYSIAINYRKXXXXXXXXXXXHKMKWTDGLTLHRFDCHSQTNEQTVQ*MLNLAVKYNKAVQEEYQLPPQKLAIANVGMQDSKKHLDVT 486
            ++T +SFE LN RAVAVVVDPI SVK  VVIDAFRLI+   ++  Q PR TTSN G +NK +IQ+ IHG    YYS+ I+Y K           HK +W  GL +  +      N +  + M+ +A +Y+K ++EE +L  ++L    VG QD KKHL  T
Sbjct:  123 VNTQKSFEQLNNRAVAVVVDPIQSVKGKVVIDAFRLIDTGALINNQEPRQTTSNSGLMNKANIQALIHGLNRHYYSLNIDYHKTPAETKMLLNLHKEQWQSGLKMQDYQEKETENLEATKRMVAVAQQYSKRIEEEKELSEEELKTRYVGKQDPKKHLAKT 283
The following BLAST results are available for this feature:
BLAST of DY257649 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
PSDE_ARATH8.716e-6172.9626S proteasome non-ATPase regulatory subunit 14 OS... [more]
PSDE_DROME1.375e-4557.8626S proteasome non-ATPase regulatory subunit 14 OS... [more]
PSDE_MOUSE5.168e-4559.1226S proteasome non-ATPase regulatory subunit 14 OS... [more]
PSDE_HUMAN5.168e-4559.1226S proteasome non-ATPase regulatory subunit 14 OS... [more]
RPN11_SCHPO6.504e-4053.4626S proteasome regulatory subunit rpn11 OS=Schizos... [more]
PSDE_CAEEL5.154e-3751.2726S proteasome non-ATPase regulatory subunit 14 OS... [more]
PSDE_DICDI1.658e-3549.3726S proteasome non-ATPase regulatory subunit 14 OS... [more]
RPN11_ASHGO4.084e-3448.4526S proteasome regulatory subunit RPN11 OS=Ashbya ... [more]
RPN11_YEAST2.739e-3044.7226S proteasome regulatory subunit RPN11 OS=Sacchar... [more]
RPN11_CANGA1.775e-2944.7226S proteasome regulatory subunit RPN11 OS=Candida... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAK3CG12FM1 AbsCFruit Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY257649 ID=DY257649; Name=DY257649; organism=Citrus sinensis; type=EST; length=1166bp
ATATTAGACACCCATCAAAGTTTTGAAGCTTTGAATCAAAGGGCTGTTGC
TGTGGTTGTGGATCCAATCCACAGCGTGAAAAGTAATGTAGTGATTGATG
CCTTCCGCTTGATTAATCCCCAAACCATGATGCTTGTTCAATAACCACGG
CATACAACTTCCAATCTTGGTCATCTTAATAAGCCATCCATCCAATCATT
TATCCATGGCTTTTTTATACTTTATTATTCTATTGCCATCAATTACAGGA
AGAATGAACTTGAAGAAAATATGCTACTAAATCTGCACAAGATGAAATGG
ACTGATGGATTAACACTTCATCGCTTTGATTGTCATTCCCAAACCAATGA
ACAGACTGTCCAGTAGATGTTAAATTTGGCTGTCAAATATAACAAGGCAG
TTCAAGAAGAATACCAGCTGCCGCCACAGAAGCTGGCCATTGCTAATGTG
GGAATGCAAGATTCAAAGAAACACCTTGATGTAACTTGTCTCCTACTTCA
TTTCCTCTAACATATTTCTAACTTTTGGAACCATGCTTGATTCCATCATA
TTCTAGATGTTGGTTTTGTTTCCTATAGTTTCCCTAGCTTCACTCTTTTT
TTGATTATTTGTATCGGCTTTTACTATTTGAATTTTTTTCTTTCTCACTT
TAACATTTTTTATTTCTGAAGGCCCTTCCCTTTTACCATGCATGATGCTG
ATTTTTTTCTTTACTTTCTATAATTATTTGTGGCTTACTTTCTTTTTTCT
AAAATTTAAATCTTATCCTTATAACACTTTTATCTCTCTCTACTTTTTTT
TATCTCTCTTCCTTTCCTTTTTCACACTTTAACACCTCTCTTTTTTTTTT
ATCTATTTTTTTTTTTTATATAAAACTATTTTCTTTCTCCCTTTTTTTTC
ACCCAATCCATCACCTCCTCTTTTTCTTAAAATTATATTATTCTTTTTCC
TCTTCCCCCCCTTTATCTCCCCCCCCTATTTTTTTATTGCTACTTATCGT
CTTCTTATATCTTTTCTTTTTCTGCTTTCTCTCTTCCTCCGCCTCCCTCC
CTCCCTTCTTTAATTTCTCCGTTTTTGTTCTTTTATTCTTTTCCTTGTTC
TTTACAATTTAAATTATTTTTTTCTTCCTCTCCCTTCCTCCTTTTTGACT
ACGACATAAACCCCAT
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