EY702460

Overview
NameEY702460
Unique NameEY702460
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length856
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX1_PHAVU (Pyridoxal biosynthesis protein PDX1 OS=Phaseolus vulgaris GN=PDX1 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 8.061e-29
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDITVPTN 228
            HINKHNFRIPF+CGCRNLGEALRRIR+GA ++RT GEAGTGNIIEAVRHV   M DI V  N
Sbjct:  134 HINKHNFRIPFVCGCRNLGEALRRIREGAAMIRTKGEAGTGNIIEAVRHVRSVMSDIRVLRN 195

HSP 2 Score: 44.2838 bits (103), Expect = 8.061e-29
Identity = 25/80 (31.25%), Postives = 30/80 (37.50%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAAC 509
            AAP+ L+  T QL RLP VHF             MMQL C                           HYSDPE+  + +C
Sbjct:  209 AAPYDLVMQTKQLGRLPVVHFAAGGVATPADAALMMQLGCDGVFVGSGVFKSGDPAKRARAIVQAVTHYSDPEILAEVSC 288
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX1_HEVBR (Probable pyridoxal biosynthesis protein PDX1 OS=Hevea brasiliensis GN=PDX1 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.468e-27
Identity = 48/62 (77.42%), Postives = 54/62 (87.10%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDITVPTN 228
            HINKHNFRIPF+CGCRNLGEALRRIR+GA ++RT GEAGTGN+IEAVRHV   MGDI +  N
Sbjct:  131 HINKHNFRIPFVCGCRNLGEALRRIREGAAMIRTKGEAGTGNVIEAVRHVRSVMGDIRLLRN 192

HSP 2 Score: 39.2762 bits (90), Expect = 1.468e-27
Identity = 23/80 (28.75%), Postives = 29/80 (36.25%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAAC 509
            AAP+ L+  T QL RLP V F             MMQL C                           HYSDP++  + +C
Sbjct:  206 AAPYDLVMQTKQLGRLPVVQFAAGGVATPADAALMMQLGCDGVFVGSGVFKSGDPARRARAIVQAVTHYSDPDMLAEVSC 285
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX13_ARATH (Pyridoxal biosynthesis protein PDX1.3 OS=Arabidopsis thaliana GN=PDX13 PE=1 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 3.239e-27
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDITVPTN 228
            HINKHNFRIPF+CGCRNLGEALRRIR+GA ++RT GEAGTGNIIEAVRHV    GDI V  N
Sbjct:  131 HINKHNFRIPFVCGCRNLGEALRRIREGAAMIRTKGEAGTGNIIEAVRHVRSVNGDIRVLRN 192

HSP 2 Score: 39.2762 bits (90), Expect = 3.239e-27
Identity = 24/80 (30.00%), Postives = 29/80 (36.25%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAAC 509
            AAP+ L+  T QL RLP V F             MMQL C                           HYSDPE+  + +C
Sbjct:  206 AAPYDLVMQTKQLGRLPVVQFAAGGVATPADAALMMQLGCDGVFVGSGIFKSGDPARRARAIVQAVTHYSDPEMLVEVSC 285
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX1_GINBI (Probable pyridoxal biosynthesis protein PDX1 OS=Ginkgo biloba GN=PDX1 PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.670e-26
Identity = 45/57 (78.95%), Postives = 51/57 (89.47%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDI 213
            HINKHNFRIPF+CGCRNLGEALRRI +GA ++RT GEAGTGN+IEAVRHV   +GDI
Sbjct:  131 HINKHNFRIPFVCGCRNLGEALRRIAEGAAMIRTKGEAGTGNVIEAVRHVRSVLGDI 187

HSP 2 Score: 39.2762 bits (90), Expect = 2.670e-26
Identity = 22/80 (27.50%), Postives = 30/80 (37.50%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAAC 509
            AAP+ L++ T QL RLP V+F             MMQL C                           HY+DP +  + +C
Sbjct:  206 AAPYELVRQTKQLGRLPVVNFAAGGVATPADAALMMQLGCDGVFVGSGVFKSGDPARRARAIVQAVTHYNDPHILAEVSC 285
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX11_ORYSJ (Probable pyridoxal biosynthesis protein PDX1.1 OS=Oryza sativa subsp. japonica GN=PDX11 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 9.970e-26
Identity = 45/62 (72.58%), Postives = 53/62 (85.48%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDITVPTN 228
            HINKHNFR+PF+CGCR+LGEALRRIR+GA ++RT GEAGTGN++EAVRHV   MGDI    N
Sbjct:  140 HINKHNFRVPFVCGCRDLGEALRRIREGAAMIRTKGEAGTGNVVEAVRHVRSVMGDIRALRN 201

HSP 2 Score: 36.1946 bits (82), Expect = 9.970e-26
Identity = 22/79 (27.85%), Postives = 28/79 (35.44%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAA 506
            AAP+ L+  T QL RLP V F             MMQL C                           HYSDP++  + +
Sbjct:  215 AAPYDLVMQTKQLGRLPVVQFAAGGVATPADAALMMQLGCDGVFVGSGIFKSGDPARRARAIVQAVTHYSDPKILAEVS 293
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX12_ORYSJ (Probable pyridoxal biosynthesis protein PDX1.2 OS=Oryza sativa subsp. japonica GN=PDX12 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.213e-25
Identity = 43/57 (75.44%), Postives = 52/57 (91.23%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDI 213
            HINK+NFR+PF+CGCR+LGEALRRIR+GA ++RT GEAGTGN++EAVRHV   MGDI
Sbjct:  133 HINKNNFRVPFVCGCRDLGEALRRIREGAAMIRTKGEAGTGNVVEAVRHVRSVMGDI 189

HSP 2 Score: 36.1946 bits (82), Expect = 8.213e-25
Identity = 22/79 (27.85%), Postives = 28/79 (35.44%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAA 506
            AAP+ L+  T QL RLP V F             MMQL C                           HYSDP++  + +
Sbjct:  208 AAPYDLVMQTKQLGRLPVVQFAAGGVATPADAALMMQLGCDGVFVGSGIFKSGDPALRARAIVQAVTHYSDPKILAEVS 286
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX11_ARATH (Pyridoxal biosynthesis protein PDX1.1 OS=Arabidopsis thaliana GN=PDX11 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 9.406e-23
Identity = 43/57 (75.44%), Postives = 50/57 (87.72%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDI 213
            HINKHNF+IPF+CGCRNLGEALRRIR+GA ++RT GEAGTGN++EAVRHV    G I
Sbjct:  132 HINKHNFKIPFVCGCRNLGEALRRIREGAAMIRTKGEAGTGNVVEAVRHVRSVNGAI 188

HSP 2 Score: 31.187 bits (69), Expect = 9.406e-23
Identity = 16/40 (40.00%), Postives = 19/40 (47.50%), Query Frame = 3 
Query:  270 AAPHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLAC 389
            AAP+ L+  T +L RLP V F             MMQL C
Sbjct:  207 AAPYDLVVQTKELGRLPVVQFAAGGVATPADAALMMQLGC 246
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDX1_EMENI (Pyridoxine biosynthesis protein pyroA OS=Emericella nidulans GN=pyroA PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 4.253e-19
Identity = 34/50 (68.00%), Postives = 45/50 (90.00%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHV 192
            H+ KHNF+ PF+CGCRNLGEALRRI +GA ++RT GEAGTG+++EAV+H+
Sbjct:  119 HVTKHNFKAPFVCGCRNLGEALRRISEGAAMIRTKGEAGTGDVVEAVKHM 168

HSP 2 Score: 30.8018 bits (68), Expect = 4.253e-19
Identity = 19/78 (24.36%), Postives = 26/78 (33.33%), Query Frame = 3 
Query:  273 APHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEVRGDAA 506
            AP+ LL+   +  RLP V+F             MMQL C                           HY DP+V  + +
Sbjct:  201 APYELLREAAEKGRLPVVNFAAGGVATPADAALMMQLGCDGVFVGSGIFKSGDAKKRAKAIVQAVTHYKDPKVLAEVS 278
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDXS_ROSS1 (Pyridoxal biosynthesis lyase pdxS OS=Roseiflexus sp. (strain RS-1) GN=pdxS PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 7.200e-19
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDI 213
            HINKH FR+PF+CGCRNLGEALRR+ +GA ++RT GEAGTGN++EAVRH      +I
Sbjct:  114 HINKHKFRVPFVCGCRNLGEALRRVAEGAAMLRTKGEAGTGNVVEAVRHARAVYSEI 170

HSP 2 Score: 25.0238 bits (53), Expect = 7.200e-19
Identity = 18/80 (22.50%), Postives = 27/80 (33.75%), Query Frame = 3 
Query:  264 TYA----APHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEV 491
            TYA    AP+ L++   +  RLP V+F             +MQL                             HY++PE+
Sbjct:  183 TYAKNIQAPYELVRQVAETGRLPVVNFAAGGIATPADAALLMQLGVDGVFVGSGIFKSGDPARRARAIVAATTHYNEPEI 262
[Close]
BLAST of EY702460 vs. ExPASy Swiss-Prot
Match: PDXS_CHLAD (Pyridoxal biosynthesis lyase pdxS OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) GN=pdxS PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.969e-19
Identity = 39/57 (68.42%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:   43 HINKHNFRIPFLCGCRNLGEALRRIRQGAGVVRTHGEAGTGNIIEAVRHVPPCMGDI 213
            HINKH FR+PF+CGCRNLGEALRRI +GA ++RT GEAGTGN++EAVRH      +I
Sbjct:  114 HINKHKFRVPFVCGCRNLGEALRRIAEGAAMLRTKGEAGTGNVVEAVRHARAVYSEI 170

HSP 2 Score: 23.483 bits (49), Expect = 7.969e-19
Identity = 18/80 (22.50%), Postives = 26/80 (32.50%), Query Frame = 3 
Query:  264 TYA----APHHLLQPTTQLARLPAVHFRXXXXXXXXXXXXMMQLACRRSLCXXXXXXXXXXXXXXXXXXXXXXHYSDPEV 491
            TYA    AP+ L++      +LP V+F             +MQL                             HY+DPE+
Sbjct:  183 TYAKQIQAPYELVKQVATEGKLPVVNFAAGGIATPADAALLMQLGVDGIFVGSGIFKSGNPIKRARAIVEATTHYNDPEI 262

HSP 3 Score: 20.7866 bits (42), Expect = 7.969e-19
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 2 
Query:   14 EVLTPADQE 40
            EVLTPAD+E
Sbjct:  104 EVLTPADEE 112
The following BLAST results are available for this feature:
BLAST of EY702460 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 201
Match NameE-valueIdentityDescription
PDX1_PHAVU8.061e-2979.03Pyridoxal biosynthesis protein PDX1 OS=Phaseolus v... [more]
PDX1_HEVBR1.468e-2777.42Probable pyridoxal biosynthesis protein PDX1 OS=He... [more]
PDX13_ARATH3.239e-2779.03Pyridoxal biosynthesis protein PDX1.3 OS=Arabidops... [more]
PDX1_GINBI2.670e-2678.95Probable pyridoxal biosynthesis protein PDX1 OS=Gi... [more]
PDX11_ORYSJ9.970e-2672.58Probable pyridoxal biosynthesis protein PDX1.1 OS=... [more]
PDX12_ORYSJ8.213e-2575.44Probable pyridoxal biosynthesis protein PDX1.2 OS=... [more]
PDX11_ARATH9.406e-2375.44Pyridoxal biosynthesis protein PDX1.1 OS=Arabidops... [more]
PDX1_EMENI4.253e-1968.00Pyridoxine biosynthesis protein pyroA OS=Emericell... [more]
PDXS_ROSS17.200e-1966.67Pyridoxal biosynthesis lyase pdxS OS=Roseiflexus s... [more]
PDXS_CHLAD7.969e-1968.42Pyridoxal biosynthesis lyase pdxS OS=Chloroflexus ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-007-B12-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702460 ID=EY702460; Name=EY702460; organism=Citrus sinensis; type=EST; length=856bp
CCACGCGTCCGGCGAAGTCTTGACTCCCGCCGACCAAGAGATCACATCAA
CAAGCACAACTTTCGTATCCCCTTCCTATGCGGCTGCCGGAACTTGGGGG
AAGCTTTGAGGAGGATTCGCCAAGGCGCGGGGGTGGTAAGAACGCACGGC
GAGGCCGGAACGGGGAATATTATCGAAGCGGTGAGGCACGTGCCGCCCTG
CATGGGGGATATTACGGTTCCGACGAACCACGGACGATGACCACGCGCTC
CCTTTCCCAAACAACATACGCGGCGCCCCACCACTTGCTGCAGCCCACCA
CGCAGCTGGCGCGGTTGCCCGCGGTGCACTTCCGAGCGGGAGGAGCGGGG
ACCCCGGCGGATCCAGCGACGATGATGCAACTTGCATGCCGACGGAGTCT
CTGCCCGCCCGGGCGCCCTCAAGAGCCGCGACCCCCCCAAGCGCCCCCAC
CCCCTCCGACCCCCGGCGCCCACTACAGTGACCCGGAGGTGCGGGGGGAC
GCGGCCTGCCGCGCCGCGCCAGGCCCCCGCCGGTCCCACTTTGAGCGACC
ACAAGGGGTTACGGTCGCCTAGCCCCCCCCATACCCGACCCACACCACCG
CCCCCGCCCCGCCATCCATGCACCACCCCATCTCTACCGCCCGCCCTCCG
GGCGGCGGACCCCCTCCCAACTTCACAAAGCCTCCTCGTTGCCCCTCCCC
GCCCCCCCCCCCCTCTCCAGCAACATACCTCACCCCCTCCCCCACGCGTC
GCGCTCCCCGCGATCCCCCCGGCTGCACCTCACTACTCCGCCTCCCCCCC
CCGCCCCCCCCCCCGCCTCCCCCCGACCACCCCCCCCGCCGCCCTTCCCC
CCCCCC
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