EY702316

Overview
NameEY702316
Unique NameEY702316
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3386 contig Csv1_Contig3386:258..914. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA1_ORYSJ (Tubulin alpha-1 chain OS=Oryza sativa subsp. japonica GN=TUBA1 PE=1 SV=1)

HSP 1 Score: 254.603 bits (649), Expect = 3.309e-67
Identity = 121/131 (92.37%), Postives = 127/131 (96.95%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDDDN-EGEDY 401
            RGDVVPKDVN AVATIKTKR VQFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD+N +GEDY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDENDDGEDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA3_ELEIN (Tubulin alpha-3 chain OS=Eleusine indica GN=TUBA3 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 5.644e-67
Identity = 121/131 (92.37%), Postives = 127/131 (96.95%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDDD-NEGEDY 401
            RGDVVPKDVN AVATIKTKR VQFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD+ +EGEDY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEGEDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA6_MAIZE (Tubulin alpha-6 chain OS=Zea mays GN=TUBA6 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.257e-66
Identity = 120/131 (91.60%), Postives = 127/131 (96.95%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDDD-NEGEDY 401
            RGDVVPKDVN AVATIKTKR VQFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD+ +EG+DY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEGDDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA5_MAIZE (Tubulin alpha-5 chain OS=Zea mays GN=TUBA5 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.257e-66
Identity = 120/131 (91.60%), Postives = 127/131 (96.95%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDDD-NEGEDY 401
            RGDVVPKDVN AVATIKTKR VQFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD+ +EG+DY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEGDDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA3_ARATH (Tubulin alpha-3/alpha-5 chain OS=Arabidopsis thaliana GN=TUBA3 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.257e-66
Identity = 121/131 (92.37%), Postives = 126/131 (96.18%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDD-DNEGEDY 401
            RGDVVPKDVN AV TIKTKR VQFVDWCPTGFKCGINYQPPTVVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD ++EGEDY
Sbjct:  320 RGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEEDEGEDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA1_HORVU (Tubulin alpha-1 chain OS=Hordeum vulgare GN=TUBA1 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.257e-66
Identity = 120/131 (91.60%), Postives = 127/131 (96.95%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDDD-NEGEDY 401
            RGDVVPKDVN AVATIKTKR VQFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD+ +EG+DY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEGDDY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA1_ARATH (Tubulin alpha-1 chain OS=Arabidopsis thaliana GN=TUBA1 PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 1.064e-65
Identity = 118/131 (90.08%), Postives = 124/131 (94.66%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGV-DDDNEGEDY 401
            RGDVVPKDVNTAVA IK KR +QFVDWCPTGFKCGINYQPP+VVPGGDLA+VQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVG +G  DDD EG++Y
Sbjct:  320 RGDVVPKDVNTAVAAIKAKRTIQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGGEGAEDDDEEGDEY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA6_ARATH (Tubulin alpha-6 chain OS=Arabidopsis thaliana GN=TUBA6 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 1.390e-65
Identity = 118/131 (90.08%), Postives = 126/131 (96.18%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDD-DNEGEDY 401
            RGDVVPKDVN AV TIKTKR +QFVDWCPTGFKCGINYQPPTVVPGGDLA+VQRAVCMISN+T+VAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD D+EGE+Y
Sbjct:  320 RGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDDEGEEY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA2_ARATH (Tubulin alpha-2/alpha-4 chain OS=Arabidopsis thaliana GN=TUBA2 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 1.390e-65
Identity = 118/131 (90.08%), Postives = 126/131 (96.18%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVDD-DNEGEDY 401
            RGDVVPKDVN AV TIKTKR +QFVDWCPTGFKCGINYQPPTVVPGGDLA+VQRAVCMISN+T+VAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+G DD D+EGE+Y
Sbjct:  320 RGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEDDEGEEY 450
[Close]
BLAST of EY702316 vs. ExPASy Swiss-Prot
Match: TBA_PRUDU (Tubulin alpha chain OS=Prunus dulcis GN=TUBA PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 6.900e-65
Identity = 116/131 (88.55%), Postives = 126/131 (96.18%), Query Frame = 3 
Query:   12 RGDVVPKDVNTAVATIKTKRAVQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCMISNNTAVAEVFSRIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGADGVD-DDNEGEDY 401
            RGDVVPKDVN AVATIKTKR +QFVDWCPTGFKCGINYQPPTVVPGGDLA+VQRAVCMISN+T+VAEVFSRIDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGA+  + +D+EG+DY
Sbjct:  320 RGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESAEGEDDEGDDY 450
The following BLAST results are available for this feature:
BLAST of EY702316 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 377
Match NameE-valueIdentityDescription
TBA1_ORYSJ3.309e-6792.37Tubulin alpha-1 chain OS=Oryza sativa subsp. japon... [more]
TBA3_ELEIN5.644e-6792.37Tubulin alpha-3 chain OS=Eleusine indica GN=TUBA3 ... [more]
TBA6_MAIZE1.257e-6691.60Tubulin alpha-6 chain OS=Zea mays GN=TUBA6 PE=2 SV... [more]
TBA5_MAIZE1.257e-6691.60Tubulin alpha-5 chain OS=Zea mays GN=TUBA5 PE=2 SV... [more]
TBA3_ARATH1.257e-6692.37Tubulin alpha-3/alpha-5 chain OS=Arabidopsis thali... [more]
TBA1_HORVU1.257e-6691.60Tubulin alpha-1 chain OS=Hordeum vulgare GN=TUBA1 ... [more]
TBA1_ARATH1.064e-6590.08Tubulin alpha-1 chain OS=Arabidopsis thaliana GN=T... [more]
TBA6_ARATH1.390e-6590.08Tubulin alpha-6 chain OS=Arabidopsis thaliana GN=T... [more]
TBA2_ARATH1.390e-6590.08Tubulin alpha-2/alpha-4 chain OS=Arabidopsis thali... [more]
TBA_PRUDU6.900e-6588.55Tubulin alpha chain OS=Prunus dulcis GN=TUBA PE=2 ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-005-F03-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702316 ID=EY702316; Name=EY702316; organism=Citrus sinensis; type=EST; length=657bp
CCACGCGTCCGCGTGGAGATGTTGTACCAAAGGATGTCAATACTGCGGTT
GCCACCATCAAGACGAAGCGGGCAGTTCAGTTTGTTGATTGGTGTCCAAC
TGGTTTCAAATGTGGTATCAACTACCAGCCACCGACAGTGGTACCAGGGG
GTGATCTTGCTAGAGTTCAGCGTGCAGTTTGTATGATAAGCAACAACACA
GCTGTGGCTGAGGTTTTCTCCCGAATTGACCATAAATTTGATCTCATGTA
TTCCAAGCGAGCATTCGTTCACTGGTATGTAGGTGAGGGCATGGAGGAAG
GTGAATTCTCAGAAGCTCGAGAAGACCTTGCTGCTCTTGAGAAAGACTAT
GAGGAAGTTGGGGCAGACGGTGTTGATGATGACAATGAGGGAGAAGATTA
CTGAAAATAATCTTGACAGGGTAGCAGCAACTGTTTCTTTATCCTATCTC
ATCTCCCATATTTTTTCTCCAGCTATGTATTGTCTGAATTCCTATCCATT
CTATTGATCTTCTAAAGTGCCTCTGTGTTGTTGTATGGCTTTGTAATTAT
GTTGGTTTTAGAAGAACAGTGTGAGAATCAACAGTCCTCATTCTTAGAAA
TGAGGCATCTCACTGCCTAATAAAATCACTTCCTTGTGCGCCCAAAAAAA
AAAAAAA
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