DR403932

Overview
NameDR403932
Unique NameDR403932
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length680
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig528 contig Csv1_Contig528:84..764. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBC35_ARATH (Ubiquitin carrier protein E2 35 OS=Arabidopsis thaliana GN=UBC35 PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.568e-73
Identity = 70/76 (92.11%), Postives = 75/76 (98.68%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYAS 548
            +IYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLS+NIAKHWK++E EAV+TAKEWTRLYAS
Sbjct:   76 KIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYAS 151

HSP 2 Score: 146.362 bits (368), Expect = 4.568e-73
Identity = 69/76 (90.79%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   97 MASGNLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            MA+ NLPRRI+KETQRLLSEPAPGISASP+EDNMRYFNVMILGP+QSPYEGGVFKLELFLPE+YPM+APKVRFLTK
Sbjct:    1 MANSNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTK 76
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBC36_ARATH (Ubiquitin carrier protein E2 36 OS=Arabidopsis thaliana GN=UBC36 PE=1 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 5.957e-73
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYAS 548
            +IYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLS+NIAKHWK++E EAVETAKEWTRLYAS
Sbjct:   76 KIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVETAKEWTRLYAS 151

HSP 2 Score: 144.821 bits (364), Expect = 5.957e-73
Identity = 68/76 (89.47%), Postives = 75/76 (98.68%), Query Frame = 1 
Query:   97 MASGNLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            MA+ NLPRRI+KETQRLLSEPAPGISASP+E+NMRYFNVMILGP+QSPYEGGVFKLELFLPE+YPM+APKVRFLTK
Sbjct:    1 MANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTK 76
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_DICDI (Probable ubiquitin-conjugating enzyme E2 N OS=Dictyostelium discoideum GN=ube2n PE=3 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.684e-60
Identity = 62/75 (82.67%), Postives = 71/75 (94.67%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+HWK +E EA+  A+EWTRLYA
Sbjct:   74 KIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEHWKTNEKEAMARAREWTRLYA 148

HSP 2 Score: 117.857 bits (294), Expect = 1.684e-60
Identity = 55/71 (77.46%), Postives = 62/71 (87.32%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LP+RI+KETQRL++EPAPGISA+PA DN RYF V I GP  SP+EGGVF LELFL +DYPMSAPKVRFLTK
Sbjct:    4 LPKRIIKETQRLMTEPAPGISATPATDNFRYFKVAISGPLDSPFEGGVFNLELFLTDDYPMSAPKVRFLTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 4.870e-60
Identity = 60/75 (80.00%), Postives = 72/75 (96.00%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK++E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 4.870e-60
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.350e-60
Identity = 60/75 (80.00%), Postives = 71/75 (94.67%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK +E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 6.350e-60
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.350e-60
Identity = 60/75 (80.00%), Postives = 71/75 (94.67%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK +E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 6.350e-60
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.350e-60
Identity = 60/75 (80.00%), Postives = 71/75 (94.67%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK +E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 6.350e-60
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_BOVIN (Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN=UBE2N PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 6.350e-60
Identity = 60/75 (80.00%), Postives = 71/75 (94.67%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK +E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 6.350e-60
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_MACFA (Ubiquitin-conjugating enzyme E2 N OS=Macaca fascicularis GN=UBE2N PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 3.120e-59
Identity = 59/75 (78.67%), Postives = 70/75 (93.33%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPN+DK GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL++++A+ WK +E +A+ETA+ WTRLYA
Sbjct:   74 KIYHPNVDKSGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYA 148

HSP 2 Score: 117.087 bits (292), Expect = 3.120e-59
Identity = 53/71 (74.65%), Postives = 61/71 (85.92%), Query Frame = 1 
Query:  112 LPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            LPRRI+KETQRLL+EP PGI A P E N RYF+V+I GP  SP+EGG FKLELFLPE+YPM+APKVRF+TK
Sbjct:    4 LPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTK 74
[Close]
BLAST of DR403932 vs. ExPASy Swiss-Prot
Match: UBE2N_DROME (Ubiquitin-conjugating enzyme E2 N OS=Drosophila melanogaster GN=ben PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 6.914e-59
Identity = 56/75 (74.67%), Postives = 69/75 (92.00%), Query Frame = 3 
Query:  321 QIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNIAKHWKADETEAVETAKEWTRLYA 545
            +IYHPNID+LGRICLD+LKDKWSPALQIRT+LLSIQALLSAPNPDDPL++++A+ WK +E EA+  A+EWT+ YA
Sbjct:   74 KIYHPNIDRLGRICLDVLKDKWSPALQIRTILLSIQALLSAPNPDDPLANDVAELWKVNEAEAIRNAREWTQKYA 148

HSP 2 Score: 120.553 bits (301), Expect = 6.914e-59
Identity = 54/72 (75.00%), Postives = 65/72 (90.28%), Query Frame = 1 
Query:  109 NLPRRIVKETQRLLSEPAPGISASPAEDNMRYFNVMILGPSQSPYEGGVFKLELFLPEDYPMSAPKVRFLTK 324
            +LPRRI+KETQRL+ EP PGI+A P E+N RYF+V++ GP+ SP+EGGVFKLELFLPEDYPMSAPKVRF+TK
Sbjct:    3 SLPRRIIKETQRLMQEPVPGINAIPDENNARYFHVIVTGPNDSPFEGGVFKLELFLPEDYPMSAPKVRFITK 74
The following BLAST results are available for this feature:
BLAST of DR403932 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 224
Match NameE-valueIdentityDescription
UBC35_ARATH4.568e-7392.11Ubiquitin carrier protein E2 35 OS=Arabidopsis tha... [more]
UBC36_ARATH5.957e-7393.42Ubiquitin carrier protein E2 36 OS=Arabidopsis tha... [more]
UBE2N_DICDI1.684e-6082.67Probable ubiquitin-conjugating enzyme E2 N OS=Dict... [more]
UBE2N_RAT4.870e-6080.00Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBE2N_PONAB6.350e-6080.00Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ... [more]
UBE2N_MOUSE6.350e-6080.00Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ... [more]
UBE2N_HUMAN6.350e-6080.00Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ... [more]
UBE2N_BOVIN6.350e-6080.00Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN... [more]
UBE2N_MACFA3.120e-5978.67Ubiquitin-conjugating enzyme E2 N OS=Macaca fascic... [more]
UBE2N_DROME6.914e-5974.67Ubiquitin-conjugating enzyme E2 N OS=Drosophila me... [more]

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Properties
Property NameValue
Genbank descriptionCSAH-PNP1246L20 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone CSAH-PNP1246L20 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403932 ID=DR403932; Name=DR403932; organism=Citrus sinensis; type=EST; length=680bp
AGGGACAAGTCACGAGAACCATAAGAGGAAAAGAGGGAGAGGGAAAAAAA
NNNNNNCGATTTCTTACTCATCTCTGTAAAATCTCTTGGAATTCCGATGG
CTAGTGGCAACCTCCCTCGCAGAATTGTCAAGGAAACCCAGCGGCTGCTC
AGTGAACCAGCTCCAGGCATTAGTGCTTCTCCTGCCGAAGACAACATGCG
ATATTTTAATGTAATGATTCTTGGTCCATCTCAGTCTCCTTATGAAGGTG
GGGTTTTCAAGTTGGAGTTGTTTTTGCCTGAAGATTATCCAATGTCCGCT
CCTAAGGTTCGATTTCTGACCAAATATATCATCCTAACATAGACAAGCTT
GGAAGGATATGCCTTGATATTTTGAAAGATAAATGGAGTCCCGCCCTGCA
AATCCGCACAGTCCTGCTGAGTATCCAAGCTCTTTTGAGTGCTCCGAACC
CAGACGATCCACTTTCTGATAACATTGCAAAGCATTGGAAGGCAGATGAG
ACTGAAGCTGTTGAAACTGCGAAGGAATGGACCCGGTTATATGCAAGTGA
TGGGTGAAGGCTAGAGCTTGTTCGAGGATATTTCTCTACAAGTTTATGTT
AAAAACACTAATTGATGTATGAACAGATGTGTAAAAATGTTTCTCAATCG
GTCTGTTATTTGAATGTTCCACTTGTATCA
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