DR403828 | Citrus Genome Database

Overview


Name	DR403828
Unique Name	DR403828
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	684

Libraries


Library Name	Type
Developing fruit peel at 38 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig7528	contig	Csv1_Contig7528:25..710.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP25_ARATH (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 7.114e-72
Identity = 108/126 (85.71%), Postives = 115/126 (91.27%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYKSQTDPA N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV+LVRAVMYMVAQCLGAI GV LVKAFQ +Y+TRYGGGAN L+DGYS G G+AAEIIGTFVLVYTV
Sbjct:   59 IGYKSQTDPALNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAVMYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTV 184

HSP 2 Score: 69.3218 bits (168), Expect = 7.114e-72
Identity = 33/46 (71.74%), Postives = 35/46 (76.09%), Query Frame = 1

Query:  148 VAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            V +  S+S KDY DPPPEPLFDA EL KWSFYRALIAEFI   L L
Sbjct:    6 VGDKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFL 51

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP22_ORYSJ (Probable aquaporin PIP2-2 OS=Oryza sativa subsp. japonica GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 9.239e-69
Identity = 104/128 (81.25%), Postives = 112/128 (87.50%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK Q+D   N     C+GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSL+RAV+Y++AQCLGAI GVGLVK FQ SYY RYGGGANEL+DGYS G GL AEIIGTFVLVYTV
Sbjct:   62 IGYKHQSDATVNTTDAACSGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYIIAQCLGAICGVGLVKGFQSSYYARYGGGANELSDGYSKGTGLGAEIIGTFVLVYTV 189

HSP 2 Score: 68.5514 bits (166), Expect = 9.239e-69
Identity = 35/54 (64.81%), Postives = 37/54 (68.52%), Query Frame = 1

Query:  130 MAKD--TGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD      E G +SAKDYTDPPP PL D  ELTKWS YRA+IAEFI   L L
Sbjct:    1 MAKDIEASAPEGGEFSAKDYTDPPPAPLIDVEELTKWSLYRAVIAEFIATLLFL 54

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP23_ORYSJ (Probable aquaporin PIP2-3 OS=Oryza sativa subsp. japonica GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.571e-68
Identity = 104/128 (81.25%), Postives = 113/128 (88.28%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK Q+DP  N     C+GVGILGIAWAFGGMIF+LVYCTAG+SGGHINPAVTFGLFLARKVSLVRAV+Y+VAQ LGAI GVGLVK FQ ++Y RYGGGANEL+DGYS G GLAAEIIGTFVLVYTV
Sbjct:   63 IGYKHQSDPGANAADAACSGVGILGIAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFLARKVSLVRAVLYIVAQSLGAICGVGLVKGFQSAFYVRYGGGANELSDGYSKGTGLAAEIIGTFVLVYTV 190

HSP 2 Score: 69.3218 bits (168), Expect = 1.571e-68
Identity = 35/55 (63.64%), Postives = 38/55 (69.09%), Query Frame = 1

Query:  130 MAKD---TGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD      AE G Y AKDY+DPPP PL DA ELTKWS YRA+IAEF+   L L
Sbjct:    1 MAKDIEAAAAAEGGEYMAKDYSDPPPAPLIDAEELTKWSLYRAVIAEFVATLLFL 55

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 2.672e-68
Identity = 105/128 (82.03%), Postives = 113/128 (88.28%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK QTD A +     C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSLVRA++Y++AQCLGAI GVGLVK FQ +YY RYGGGANEL+DGYS G GLAAEIIGTFVLVYTV
Sbjct:   63 IGYKHQTDAAASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSKGTGLAAEIIGTFVLVYTV 190

HSP 2 Score: 67.3958 bits (163), Expect = 2.672e-68
Identity = 32/44 (72.73%), Postives = 34/44 (77.27%), Query Frame = 1

Query:  154 EHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            E G +SAKDYTDPPP PL DA ELTKWS YRA+IAEFI   L L
Sbjct:   12 EAGEFSAKDYTDPPPAPLIDADELTKWSLYRAVIAEFIATLLFL 55

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 3.485e-68
Identity = 104/128 (81.25%), Postives = 113/128 (88.28%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK QTD + +     C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSLVRA++Y++AQCLGAI GVGLVK FQ +YY RYGGGANEL+DGYS G GLAAEIIGTFVLVYTV
Sbjct:   62 IGYKHQTDASASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSKGTGLAAEIIGTFVLVYTV 189

HSP 2 Score: 68.1662 bits (165), Expect = 3.485e-68
Identity = 35/54 (64.81%), Postives = 39/54 (72.22%), Query Frame = 1

Query:  130 MAKDTGVA--EHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD   +  E G +SAKDYTDPPP PL DA ELT+WS YRA+IAEFI   L L
Sbjct:    1 MAKDIEASGPEAGEFSAKDYTDPPPAPLIDAEELTQWSLYRAVIAEFIATLLFL 54

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP22_ARATH (Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 216.468 bits (550), Expect = 3.801e-67
Identity = 106/126 (84.13%), Postives = 111/126 (88.10%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK Q+D       C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSL+RAV+YMVAQCLGAI GVG VKAFQ SYY RYGGGAN LADGY+TG GLAAEIIGTFVLVYTV
Sbjct:   58 IGYKIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTV 183

HSP 2 Score: 60.077 bits (144), Expect = 3.801e-67
Identity = 30/52 (57.69%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  130 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD    E   +  +DY DPPP P FDA ELTKWS YRA+IAEF+   L L
Sbjct:    1 MAKDVEGPE--GFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFL 50

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP24_ARATH (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 6.462e-67
Identity = 105/126 (83.33%), Postives = 109/126 (86.51%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK+QTD       C GVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVT GLFLARKVSLVR V+Y+VAQCLGAI G G VKAFQ SYYTRYGGGANELADGY+ G GL AEIIGTFVLVYTV
Sbjct:   60 IGYKAQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTVGLFLARKVSLVRTVLYIVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFVLVYTV 185

HSP 2 Score: 62.7734 bits (151), Expect = 6.462e-67
Identity = 30/52 (57.69%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  130 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD  V E G  +A+DY DPPP P FD  EL KW  YRA+IAEF+   L L
Sbjct:    1 MAKDLDVNESGPPAARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFL 52

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 1.433e-66
Identity = 105/126 (83.33%), Postives = 111/126 (88.10%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK Q+D       C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSL RA++Y++AQCLGAI GVG VKAFQ SYYTRYGGGAN LADGYSTG GLAAEIIGTFVLVYTV
Sbjct:   60 IGYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTV 185

HSP 2 Score: 60.077 bits (144), Expect = 1.433e-66
Identity = 27/52 (51.92%), Postives = 31/52 (59.62%), Query Frame = 1

Query:  130 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD        +  +DY DPPP P  D  EL KWSFYRA+IAEF+   L L
Sbjct:    1 MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFL 52

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP23_ARATH (Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 9.190e-66
Identity = 104/126 (82.54%), Postives = 110/126 (87.30%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK Q+D       C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLFLARKVSL+RAV+YMVAQCLGAI GVG VKAFQ S+Y  YGGGAN LADGY+TG GLAAEIIGTFVLVYTV
Sbjct:   58 IGYKIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTV 183

HSP 2 Score: 60.077 bits (144), Expect = 9.190e-66
Identity = 30/52 (57.69%), Postives = 34/52 (65.38%), Query Frame = 1

Query:  130 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            MAKD  V     +  +DY DPPP P FDA ELTKWS YRA+IAEF+   L L
Sbjct:    1 MAKD--VEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFL 50

[Close]

BLAST of DR403828 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.562e-65
Identity = 103/130 (79.23%), Postives = 112/130 (86.15%), Query Frame = 2

Query:  305 IGYKSQTDPAKNVDG----CAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKSYYTRYGGGANELADGYSTGVGLAAEIIGTFVLVYTV 682
            IGYK QTD + +  G    C GVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRA++YMVAQCLGA+ GVGLVKAFQ +Y+ RYGGGAN LA GYS G GL AEI+GTFVLVYTV
Sbjct:   63 IGYKHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSRGAGLGAEIVGTFVLVYTV 192

HSP 2 Score: 62.003 bits (149), Expect = 1.562e-65
Identity = 33/55 (60.00%), Postives = 36/55 (65.45%), Query Frame = 1

Query:  130 MAKDTGV---AEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFINPALSL 285
            M KD  V   A  G ++AKDYTDPPP PL DA EL  WS YRA+IAEFI   L L
Sbjct:    1 MGKDDVVQSGAGGGEFAAKDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFL 55

The following BLAST results are available for this feature:

BLAST of DR403828 vs. ExPASy Swiss-Prot

BLAST of DR403828 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 89


Match Name	E-value	Identity	Description
PIP25_ARATH	7.114e-72	85.71	Probable aquaporin PIP2-5 OS=Arabidopsis thaliana ...	[more]
PIP22_ORYSJ	9.239e-69	81.25	Probable aquaporin PIP2-2 OS=Oryza sativa subsp. j...	[more]
PIP23_ORYSJ	1.571e-68	81.25	Probable aquaporin PIP2-3 OS=Oryza sativa subsp. j...	[more]
PIP23_MAIZE	2.672e-68	82.03	Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1	[more]
PIP24_MAIZE	3.485e-68	81.25	Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1	[more]
PIP22_ARATH	3.801e-67	84.13	Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2...	[more]
PIP24_ARATH	6.462e-67	83.33	Probable aquaporin PIP2-4 OS=Arabidopsis thaliana ...	[more]
PIP21_ARATH	1.433e-66	83.33	Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1...	[more]
PIP23_ARATH	9.190e-66	82.54	Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3...	[more]
PIP22_MAIZE	1.562e-65	79.23	Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CSAH-PNP1246G12 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone CSAH-PNP1246G12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR403828 ID=DR403828; Name=DR403828; organism=Citrus sinensis; type=EST; length=684bp
CGGCCGGGGAGCAAAAAAAAAGAAAAATNNNNAAGAAAAAGGAAAAGGCT
TCTCTCTCGTTGTGCTTGTGGCTTTGCAGTGCTCTCAGTTTTCTCTGTTT
CTCTCCAAATCATTGTTACTGGTTTTCCAATGGCGAAGGACACTGGGGTG
GCAGAGCACGGCTCATATTCAGCCAAGGACTACACGGACCCACCGCCGGA
GCCGCTATTCGACGCCGTGGAGCTGACCAAATGGTCATTCTACAGAGCCT
TGATAGCTGAGTTCATCAACCCTGCTCTTTCTTTACGTCACTGTCTTGAC
TGTCATTGGCTACAAAAGCCAGACCGATCCCGCCAAGAACGTCGACGGCT
GTGCTGGCGTCGGCATCCTCGGCATCGCCTGGGCCTTCGGTGGCATGATC
TTTGTCCTCGTTTACTGCACCGCCGGTATCTCAGGTGGTCACATTAACCC
GGCAGTGACGTTCGGACTATTCTTGGCCCGCAAGGTGTCGTTGGTCCGTG
CTGTGATGTACATGGTGGCGCAGTGCCTTGGTGCTATCTCCGGTGTTGGG
TTGGTCAAGGCTTTCCAAAAATCTTACTACACAAGGTACGGAGGTGGGGC
CAACGAGCTGGCCGATGGGTACAGCACCGGCGTTGGATTGGCCGCCGAGA
TCATCGGCACCTTTGTCTTGGTTTACACTGTCTT