CX069391

Overview
NameCX069391
Unique NameCX069391
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length770
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1566 contig Csv1_Contig1566:31..800. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB3_GOSHI (Tubulin beta-3 chain OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 461.84 bits (1187), Expect = 1.813e-129
Identity = 223/238 (93.70%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIG+SDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGSSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB5_WHEAT (Tubulin beta-5 chain OS=Triticum aestivum GN=TUBB5 PE=2 SV=1)

HSP 1 Score: 461.455 bits (1186), Expect = 2.368e-129
Identity = 221/238 (92.86%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB5_MAIZE (Tubulin beta-5 chain OS=Zea mays GN=TUBB5 PE=2 SV=1)

HSP 1 Score: 461.455 bits (1186), Expect = 2.368e-129
Identity = 221/238 (92.86%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB4_ELEIN (Tubulin beta-4 chain OS=Eleusine indica GN=TUBB4 PE=2 SV=1)

HSP 1 Score: 461.455 bits (1186), Expect = 2.368e-129
Identity = 221/238 (92.86%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB1_WHEAT (Tubulin beta-1 chain OS=Triticum aestivum GN=TUBB1 PE=2 SV=1)

HSP 1 Score: 461.455 bits (1186), Expect = 2.368e-129
Identity = 221/238 (92.86%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB1_ORYSJ (Tubulin beta-1 chain OS=Oryza sativa subsp. japonica GN=TUBB1 PE=2 SV=2)

HSP 1 Score: 461.455 bits (1186), Expect = 2.368e-129
Identity = 221/238 (92.86%), Postives = 224/238 (94.12%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB6_ORYSJ (Tubulin beta-6 chain OS=Oryza sativa subsp. japonica GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 459.914 bits (1182), Expect = 6.889e-129
Identity = 221/238 (92.86%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYAGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB6_ARATH (Tubulin beta-6 chain OS=Arabidopsis thaliana GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 459.144 bits (1180), Expect = 1.175e-128
Identity = 219/238 (92.02%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+G SDLQLERVNVYYNEASCGR+VPRA+LMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGNSDLQLERVNVYYNEASCGRYVPRAILMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB_HORVU (Tubulin beta chain OS=Hordeum vulgare GN=TUBB PE=2 SV=1)

HSP 1 Score: 458.759 bits (1179), Expect = 1.535e-128
Identity = 220/238 (92.44%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIG+KFWEVVCDEHGIDPTGRY GTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
[Close]
BLAST of CX069391 vs. ExPASy Swiss-Prot
Match: TBB8_MAIZE (Tubulin beta-8 chain OS=Zea mays GN=TUBB8 PE=2 SV=1)

HSP 1 Score: 458.759 bits (1179), Expect = 1.535e-128
Identity = 220/238 (92.44%), Postives = 223/238 (93.70%), Query Frame = 1 
Query:   55 MREILHVQGGQCGNQIGSKFWEVVCDEHGIDPTGRYIGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 768
            MREILH+QGGQCGNQIGSKFWEVVCDEHGIDPTGRY+GTSDLQLERVNVYYNEASCGRFVP AVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPGAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTC 238
The following BLAST results are available for this feature:
BLAST of CX069391 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 423
Match NameE-valueIdentityDescription
TBB3_GOSHI1.813e-12993.70Tubulin beta-3 chain OS=Gossypium hirsutum PE=2 SV... [more]
TBB5_WHEAT2.368e-12992.86Tubulin beta-5 chain OS=Triticum aestivum GN=TUBB5... [more]
TBB5_MAIZE2.368e-12992.86Tubulin beta-5 chain OS=Zea mays GN=TUBB5 PE=2 SV=... [more]
TBB4_ELEIN2.368e-12992.86Tubulin beta-4 chain OS=Eleusine indica GN=TUBB4 P... [more]
TBB1_WHEAT2.368e-12992.86Tubulin beta-1 chain OS=Triticum aestivum GN=TUBB1... [more]
TBB1_ORYSJ2.368e-12992.86Tubulin beta-1 chain OS=Oryza sativa subsp. japoni... [more]
TBB6_ORYSJ6.889e-12992.86Tubulin beta-6 chain OS=Oryza sativa subsp. japoni... [more]
TBB6_ARATH1.175e-12892.02Tubulin beta-6 chain OS=Arabidopsis thaliana GN=TU... [more]
TBB_HORVU1.535e-12892.44Tubulin beta chain OS=Hordeum vulgare GN=TUBB PE=2... [more]
TBB8_MAIZE1.535e-12892.44Tubulin beta-8 chain OS=Zea mays GN=TUBB8 PE=2 SV=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_10B07_g Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-10B07-C14-1-4.g, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX069391 ID=CX069391; Name=CX069391; organism=Citrus sinensis; type=EST; length=770bp
CTCACTCTCATTTTTTCTCTCTCTAGCGAAAATCTTCAGTAAGTCTCAGG
AAAAATGAGGGAGATCCTTCATGTTCAGGGTGGACAATGTGGTAACCAGA
TTGGTTCCAAGTTCTGGGAGGTTGTCTGTGATGAACATGGCATCGACCCA
ACCGGAAGATACATTGGCACCTCAGATCTGCAGTTGGAGCGTGTCAATGT
CTACTACAATGAGGCCTCCTGTGGGCGGTTTGTTCCTCGTGCAGTTCTCA
TGGATTTGGAGCCTGGCACCATGGACAGTGTTAGAACTGGTCCATACGGC
CAGATTTTCAGGCCTGATAACTTTGTGTTTGGACAGTCTGGTGCTGGAAA
CAATTGGGCCAAGGGTCACTACACTGAGGGTGCTGAGCTTATTGATGCTG
TTCTTGATGTTGTGAGAAAAGAGGCTGAGAACTGTGACTGTCTTCAAGGT
TTCCAAGTCTGCCACTCTTTGGGTGGAGGAACTGGTTCTGGAATGGGTAC
CTTGTTGATCTCAAAAATTAGGGAGGAGTATCCTGACAGGATGATGCTCA
CATTCTCAGTGTTCCCTTCTCCAAAGGTCTCCGATACAGTTGTTGAGCCC
TACAATGCAACCCTCTCTGTTCACCAGCTTGTTGAGAATGCAGATGAGTG
CATGGTCCTTGATAATGAGGCTTTGTATGACATCTGCTTCAGAACTCTTA
AATTGACCACACCAAGCTTTGGTGACTTGAACCACTTGATTTCTGCAACC
ATGAGTGGCGTCACTTGCTG
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