DR403720

Overview
NameDR403720
Unique NameDR403720
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length731
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13571 contig Csv1_Contig13571:55..784. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX1_PEA (Phospholipid hydroperoxide glutathione peroxidase, chloroplastic OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 4.512e-77
Identity = 133/203 (65.52%), Postives = 148/203 (72.91%), Query Frame = 1 
Query:   46 SMPFSAAFSSPLRHFTQIKTCPXXXXXXXXXXXTPTNSI---KSSIGSAKPGFLQHGLFSQ-SSNLPGFFVKRRSFGVHATAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            SM FS  F +PLR F Q +T             TP+ S+   KSSI S+K  F Q G   Q SSN P    K RSF V+A A  +K++YDFTVKDID KDV LSKFKGKVLLIVNVASRCGLT SNY+ELSHLYE +K +G E+LAFPCNQFG QEPGSN EIK+FACT+FKAEFPIFDKVDVNGP TAPVYQFLKSS  G F
Sbjct:    3 SMAFSTTFFTPLRDFNQPRT-----------NSTPSTSLPFTKSSIASSKSPFFQLGFSQQASSNFPIVPSKTRSFSVNAKAIKDKTIYDFTVKDIDKKDVSLSKFKGKVLLIVNVASRCGLTSSNYTELSHLYENFKNKGLEVLAFPCNQFGMQEPGSNEEIKQFACTKFKAEFPIFDKVDVNGPFTAPVYQFLKSSSGGFF 194

HSP 2 Score: 64.6994 bits (156), Expect = 4.512e-77
Identity = 28/33 (84.85%), Postives = 30/33 (90.91%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGF GD+VK  FEKFLVDKNGKV+ERYPPTTSP
Sbjct:  191 GGFFGDIVKWNFEKFLVDKNGKVVERYPPTTSP 223
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX1_ARATH (Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic OS=Arabidopsis thaliana GN=GPX1 PE=1 SV=2)

HSP 1 Score: 237.269 bits (604), Expect = 9.993e-74
Identity = 118/160 (73.75%), Postives = 132/160 (82.50%), Query Frame = 1 
Query:  157 SIKSSIGSAKPGFLQHGLFSQSSNLPGFFVKRRSFGVHATAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWG 636
            S+K S G +    L +G   +S   PGF  K R F V A AA EK+++DFTVKDIDGKDV L+KFKGKV+LIVNVASRCGLT SNYSELSHLYEKYKTQGFEILAFPCNQFG QEPGSN EIK+FACTRFKAEFPIFDKVDVNGP+TAP+Y+FLKS+  G
Sbjct:   33 SLKFSTGISNFANLSNGFSLKSPINPGFLFKSRPFTVQARAAAEKTVHDFTVKDIDGKDVALNKFKGKVMLIVNVASRCGLTSSNYSELSHLYEKYKTQGFEILAFPCNQFGFQEPGSNSEIKQFACTRFKAEFPIFDKVDVNGPSTAPIYEFLKSNAGG 192

HSP 2 Score: 61.2326 bits (147), Expect = 9.993e-74
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGFLG L+K  FEKFL+DK GKV+ERYPPTTSP
Sbjct:  191 GGFLGGLIKWNFEKFLIDKKGKVVERYPPTTSP 223
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX7_ARATH (Putative glutathione peroxidase 7, chloroplastic OS=Arabidopsis thaliana GN=GPX7 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 9.996e-74
Identity = 116/160 (72.50%), Postives = 133/160 (83.12%), Query Frame = 1 
Query:  157 SIKSSIGSAKPGFLQHGLFSQSSNLPGFFVKRRSFGVHATAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWG 636
            S++ S  ++K     +G+  +SSN   F VK ++F V+A AA EKS++DFTVKDIDG DV L KFKGK LLIVNVASRCGLT SNYSELS LYEKYK QGFEILAFPCNQFGGQEPGSNPEIK+FACTRFKAEFPIFDKVDVNGP+TAP+Y+FLKS+  G
Sbjct:   30 SLRFSTRTSKTRNPSNGVSVKSSNSHRFLVKSKNFSVYARAAAEKSVHDFTVKDIDGNDVSLDKFKGKPLLIVNVASRCGLTSSNYSELSQLYEKYKNQGFEILAFPCNQFGGQEPGSNPEIKQFACTRFKAEFPIFDKVDVNGPSTAPIYKFLKSNAGG 189

HSP 2 Score: 63.5438 bits (153), Expect = 9.996e-74
Identity = 27/33 (81.82%), Postives = 30/33 (90.91%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGFLGD++K  FEKFLVDK GKV+ERYPPTTSP
Sbjct:  188 GGFLGDIIKWNFEKFLVDKKGKVVERYPPTTSP 220
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX6_ARATH (Probable phospholipid hydroperoxide glutathione peroxidase 6, mitochondrial OS=Arabidopsis thaliana GN=GPX6 PE=2 SV=2)

HSP 1 Score: 200.29 bits (508), Expect = 1.264e-58
Identity = 103/164 (62.80%), Postives = 120/164 (73.17%), Query Frame = 1 
Query:  151 TNSIKSSIGSAKPGFLQHGLFSQSSNLPGFFVKRRSFGVHATAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            ++S      SAKP F  H + S   +  G  + R    + A ++  KSLYDFTVKD  G DV LS +KGKVLLIVNVAS+CGLT SNY+EL+ LYEKYK  GFEILAFPCNQFG QEPG+N EI +FACTRFKAE+PIFDKVDVNG   APVY+FLKSS  G+F
Sbjct:   26 SSSSSKRFDSAKPLFNSHRIISLPISTTGAKLSRSEHSM-AASSEPKSLYDFTVKDAKGNDVDLSIYKGKVLLIVNVASQCGLTNSNYTELAQLYEKYKGHGFEILAFPCNQFGNQEPGTNEEIVQFACTRFKAEYPIFDKVDVNGDKAAPVYKFLKSSKGGLF 188

HSP 2 Score: 47.7506 bits (112), Expect = 1.264e-58
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GG  GD +K  F KFLVDK+G V++R+ PTTSP
Sbjct:  185 GGLFGDGIKWNFAKFLVDKDGNVVDRFAPTTSP 217
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_CITSI (Probable phospholipid hydroperoxide glutathione peroxidase OS=Citrus sinensis GN=CSA PE=1 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 2.135e-55
Identity = 88/120 (73.33%), Postives = 103/120 (85.83%), Query Frame = 1 
Query:  283 TEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            ++ S++DFTVKD  G+DV LS +KGK+LLIVNVAS+CGLT SNY+ELS LY+KYK QG EILAFPCNQFG QEPG N +I+EFACTRFKAEFPIFDKVDVNG N AP+Y+ LKSS  G+F
Sbjct:    5 SKTSVHDFTVKDAKGQDVDLSIYKGKLLLIVNVASQCGLTNSNYTELSQLYDKYKNQGLEILAFPCNQFGAQEPGDNEQIQEFACTRFKAEFPIFDKVDVNGDNAAPLYKHLKSSKGGLF 124

HSP 2 Score: 51.2174 bits (121), Expect = 2.135e-55
Identity = 22/33 (66.67%), Postives = 24/33 (72.73%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GG  GD +K  F KFLVDK G V+ERY PTTSP
Sbjct:  121 GGLFGDSIKWNFSKFLVDKEGNVVERYAPTTSP 153
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_MESCR (Probable phospholipid hydroperoxide glutathione peroxidase OS=Mesembryanthemum crystallinum GN=GPXMC1 PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 3.026e-54
Identity = 88/118 (74.58%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:  289 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            KS++DF VKD  G DV LS +KGKVLLIVNVAS+CGLT SNY EL+ LYE+YK +G EILAFPCNQFG QEPG N +I EFACTRFKAEFPIFDKVDVNG N APVY++LKSS  G+F
Sbjct:   10 KSIHDFIVKDARGNDVDLSIYKGKVLLIVNVASQCGLTNSNYPELTKLYEQYKDKGLEILAFPCNQFGNQEPGDNEQIMEFACTRFKAEFPIFDKVDVNGSNAAPVYKYLKSSKGGLF 127

HSP 2 Score: 50.0618 bits (118), Expect = 3.026e-54
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GG  GD +K  F KFLVD++GKV++RY PTTSP
Sbjct:  124 GGLFGDGIKWNFTKFLVDRDGKVVDRYAPTTSP 156
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_NICSY (Probable phospholipid hydroperoxide glutathione peroxidase OS=Nicotiana sylvestris PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 6.707e-54
Identity = 85/118 (72.03%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:  289 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            +S+YDFTVKD  G DV LS +KGKVL+IVNVAS+CGLT SNY++L+ +Y+KYK QG EILAFPCNQFGGQEPGS  EI+   CTRFKAE+PIFDKVDVNG N AP+Y+FLKSS  G F
Sbjct:    9 QSIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFF 126

HSP 2 Score: 51.2174 bits (121), Expect = 6.707e-54
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGF GD +K  F KFLVDK G V++RY PTT+P
Sbjct:  123 GGFFGDSIKWNFSKFLVDKEGNVVDRYSPTTTP 155
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_SPIOL (Probable phospholipid hydroperoxide glutathione peroxidase OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 8.740e-54
Identity = 86/123 (69.92%), Postives = 103/123 (83.74%), Query Frame = 1 
Query:  274 TAATEKSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            ++A  KS+++F V+D  G DV LS +KGKVLLIVNVAS+CGLT SNY+E++ LYEKY+  G EILAFPCNQFG QEPGSN E+ EFACTRFKAE+PIFDKVDVNG N AP+Y+FLKSS  G+F
Sbjct:    5 SSAQPKSVHEFVVRDARGNDVDLSIYKGKVLLIVNVASQCGLTNSNYTEMTELYEKYRELGLEILAFPCNQFGNQEPGSNEEVLEFACTRFKAEYPIFDKVDVNGSNAAPIYKFLKSSKGGLF 127

HSP 2 Score: 48.521 bits (114), Expect = 8.740e-54
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GG  GD +K  F KFLVDK+G V++RY PTTSP
Sbjct:  124 GGLFGDGLKWNFTKFLVDKDGNVVDRYAPTTSP 156
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_SOLLC (Probable phospholipid hydroperoxide glutathione peroxidase OS=Solanum lycopersicum GN=GPXle-1 PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 8.744e-54
Identity = 84/118 (71.19%), Postives = 101/118 (85.59%), Query Frame = 1 
Query:  289 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            +S+YDFTVKD  GKDV LS +KGKVL+IVNVAS+CGLT SNY++++ LY+KYK QG EILAFPCNQFGGQEPG+  +I++  CTRFKAE+PIFDKVDVNG N AP+Y+FLKSS  G F
Sbjct:    9 QSVYDFTVKDAKGKDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLEILAFPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKGGFF 126

HSP 2 Score: 50.8322 bits (120), Expect = 8.744e-54
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGF GD +K  F KFL+DK G V++RY PTTSP
Sbjct:  123 GGFFGDGIKWNFSKFLIDKEGHVVDRYSPTTSP 155
[Close]
BLAST of DR403720 vs. ExPASy Swiss-Prot
Match: GPX4_TOBAC (Probable phospholipid hydroperoxide glutathione peroxidase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.140e-53
Identity = 84/118 (71.19%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:  289 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYQFLKSSXWGIF 642
            +S+YDFTVKD  G DV LS +KGKVL+IVNVAS+CGLT SNY++++ +Y+KYK QG EILAFPCNQFGGQEPGS  EI+   CTRFKAE+PIFDKVDVNG N AP+Y+FLKSS  G F
Sbjct:    9 QSIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFF 126

HSP 2 Score: 51.2174 bits (121), Expect = 1.140e-53
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  632 GGFLGDLVKXXFEKFLVDKNGKVIERYPPTTSP 730
            GGF GD +K  F KFLVDK G V++RY PTT+P
Sbjct:  123 GGFFGDSIKWNFSKFLVDKEGNVVDRYSPTTTP 155
The following BLAST results are available for this feature:
BLAST of DR403720 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110
Match NameE-valueIdentityDescription
GPX1_PEA4.512e-7765.52Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX1_ARATH9.993e-7473.75Phospholipid hydroperoxide glutathione peroxidase ... [more]
GPX7_ARATH9.996e-7472.50Putative glutathione peroxidase 7, chloroplastic O... [more]
GPX6_ARATH1.264e-5862.80Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_CITSI2.135e-5573.33Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_MESCR3.026e-5474.58Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_NICSY6.707e-5472.03Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_SPIOL8.740e-5469.92Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_SOLLC8.744e-5471.19Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_TOBAC1.140e-5371.19Probable phospholipid hydroperoxide glutathione pe... [more]

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Properties
Property NameValue
Genbank descriptionCSAH-PNP1246B03 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone CSAH-PNP1246B03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR403720 ID=DR403720; Name=DR403720; organism=Citrus sinensis; type=EST; length=731bp
GGGGAACGNNNGATTCACTTAAATTCCATCTCAATGGCTTCTTATTCGAT
GCCTTTCTCTGCAGCTTTCTCCTCACCTTTACGGCATTTCACACAAATCA
AAACATGCCCTGCTGCATGGGCTTCATCAATGGCGGCTTCATGGACTCCT
ACTAATTCAATCAAATCATCAATTGGGTCCGCTAAACCAGGCTTTCTTCA
ACATGGGCTGTTTTCCCAATCTTCAAATTTGCCTGGGTTTTTCGTGAAAC
GTCGTTCCTTTGGTGTTCACGCTACAGCTGCTACTGAGAAGTCTTTATAT
GACTTCACCGTCAAGGATATTGATGGTAAAGATGTTCCTCTTAGCAAGTT
TAAGGGCAAAGTTCTGTTGATTGTGAATGTCGCTTCAAGATGTGGCTTGA
CACCATCTAATTACTCGGAGCTCTCTCACTTATATGAGAAGTACAAGACT
CAAGGATTTGAGATTCTAGCTTTCCCTTGCAATCAATTTGGTGGGCAAGA
GCCTGGATCAAACCCTGAGATCAAGGAATTCGCTTGTACTAGGTTTAAGG
CAGAATTTCCCATATTTGACAAGGTTGATGTAAATGGACCAAACACAGCT
CCAGTTTACCAGTTTTTGAAGTCAAGTGNCTGGGGGATTTTTAGGCGACC
TTGTCAAGTGNNNCTTTGAGAAGTTCTTAGTGGACAAAAATGGTAAAGTC
ATCGAGAGATATCCACCAACTACCTCGCCTT
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