EY734994

Overview
NameEY734994
Unique NameEY734994
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length738
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_SPIOL (Ferredoxin-dependent glutamate synthase, chloroplastic OS=Spinacia oleracea PE=1 SV=3)

HSP 1 Score: 186.037 bits (471), Expect = 1.785e-46
Identity = 89/99 (89.90%), Postives = 96/99 (96.97%), Query Frame = 1
Query:    1 CVVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACA 297
            CVV+LGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVK+QRV APVGQMQLK+LIEAHVEKTGSSKG++ILK+WD YLPLFWQLVPPSEEDTP+A A
Sbjct: 1404 CVVILGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKIQRVTAPVGQMQLKNLIEAHVEKTGSSKGASILKDWDKYLPLFWQLVPPSEEDTPEASA 1502          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB1_ARATH (Ferredoxin-dependent glutamate synthase 1, chloroplastic OS=Arabidopsis thaliana GN=GLU1 PE=1 SV=2)

HSP 1 Score: 179.104 bits (453), Expect = 2.506e-46
Identity = 84/105 (80.00%), Postives = 95/105 (90.48%), Query Frame = 1
Query:    1 CVVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACARVCKNS 315
            CVVVLGKVGRNVAAGMTGGLAY+LDEDDTL+PK+N+EIVK+QRV AP G++QLKSLIEAHVEKTGSSKG+ IL EW+ YLPLFWQLVPPSEEDTP+A A   + S
Sbjct: 1535 CVVVLGKVGRNVAAGMTGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEWEKYLPLFWQLVPPSEEDTPEASAAYVRTS 1639          

HSP 2 Score: 26.1794 bits (56), Expect = 2.506e-46
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 2
Query:  302 YVRTATGEVTLQS 340
            YVRT+TGEVT QS
Sbjct: 1635 YVRTSTGEVTFQS 1647          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_MAIZE (Ferredoxin-dependent glutamate synthase, chloroplastic OS=Zea mays GN=GLSF PE=1 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 3.722e-44
Identity = 85/99 (85.86%), Postives = 92/99 (92.93%), Query Frame = 1
Query:    1 CVVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACA 297
            CVVVLGK GRNVAAGMTGGLAYILDEDDTL+PKVNKEIVK+QRV AP GQMQLK LIEA+VEKTGS KG AIL+EW+ YLPLFWQLVPPSEED+P+ACA
Sbjct: 1501 CVVVLGKAGRNVAAGMTGGLAYILDEDDTLVPKVNKEIVKMQRVNAPAGQMQLKGLIEAYVEKTGSEKGIAILREWEAYLPLFWQLVPPSEEDSPEACA 1599          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB2_ARATH (Ferredoxin-dependent glutamate synthase 2, chloroplastic OS=Arabidopsis thaliana GN=GLU2 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 4.549e-42
Identity = 80/97 (82.47%), Postives = 92/97 (94.85%), Query Frame = 1
Query:    1 CVVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQA 291
            CVV+LGKVGRNVAAGMTGGLAYILDED+TL+PK+NKEIVK+QRV +PVGQ QLKSLI+AHVEKTGSSKG+ I++EWD YL +FWQLVPPSEEDTP+A
Sbjct: 1510 CVVILGKVGRNVAAGMTGGLAYILDEDNTLLPKMNKEIVKIQRVTSPVGQTQLKSLIQAHVEKTGSSKGAMIVEEWDKYLAMFWQLVPPSEEDTPEA 1606          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_PORYE (Ferredoxin-dependent glutamate synthase OS=Porphyra yezoensis GN=gltB PE=3 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 5.768e-29
Identity = 60/90 (66.67%), Postives = 71/90 (78.89%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSE 273
            +VVLGK GRNV AGMTGGLAY LDED+  I +VN EIVK+QRVI   G+ QLK+LIE H  KTGS K   IL++W++YLP FWQ+VPPSE
Sbjct: 1431 IVVLGKAGRNVGAGMTGGLAYFLDEDENFIDRVNSEIVKIQRVITKAGEEQLKNLIENHAAKTGSLKAHTILEKWNSYLPQFWQVVPPSE 1520          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_PORPU (Ferredoxin-dependent glutamate synthase OS=Porphyra purpurea GN=gltB PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.285e-28
Identity = 62/104 (59.62%), Postives = 74/104 (71.15%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACARVCKNS 315
            +VVLGK GRNV AGMTGGLAY LDE++  I +VN EIVKVQRVI   G+ QLK+LIE H  KTGS K   IL+ W+TYLP FWQ+VPPSE +  +       N+
Sbjct: 1431 IVVLGKAGRNVGAGMTGGLAYFLDEENKFIERVNSEIVKVQRVITKAGEQQLKNLIENHSAKTGSLKAHNILENWNTYLPQFWQVVPPSEANIEETNTAYSSNT 1534          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTS_SYNY3 (Ferredoxin-dependent glutamate synthase 2 OS=Synechocystis sp. (strain PCC 6803) GN=gltS PE=1 SV=2)

HSP 1 Score: 125.561 bits (314), Expect = 2.863e-28
Identity = 65/113 (57.52%), Postives = 77/113 (68.14%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACARVCKNSYWGGDIAVT 342
            +VVLG VGRNV AGMTGGLAY LDE   L  K+N EI+ +QR+ A  G+ QLKSLI AHVE TGS KG AIL  W  YL  FWQ VPPSE+D+P+A           GD+++T
Sbjct: 1446 IVVLGPVGRNVGAGMTGGLAYFLDEVGDLPEKINPEIITLQRITASKGEEQLKSLITAHVEHTGSPKGKAILANWSDYLGKFWQAVPPSEKDSPEA----------NGDVSLT 1548          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_CYACA (Ferredoxin-dependent glutamate synthase OS=Cyanidium caldarium GN=gltB PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.470e-24
Identity = 54/95 (56.84%), Postives = 69/95 (72.63%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQ 288
            VVVLGK GRN+AAGMTGG+AY LDE   L  KVN +IV++QRV+    + QL  LIE HV KTGS K   IL++W+ ++  FWQ+VPPSE +T +
Sbjct: 1443 VVVLGKAGRNIAAGMTGGIAYFLDEYSNLPEKVNLDIVRIQRVVTNEARKQLIQLIEKHVLKTGSKKAVLILQQWEIFIHYFWQIVPPSESETSE 1537          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_ANTSP (Ferredoxin-dependent glutamate synthase OS=Antithamnion sp. GN=gltB PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.390e-21
Identity = 49/91 (53.85%), Postives = 64/91 (70.33%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEE 276
            +VVLG  GRN+ AGMTGG+AY LDED+TL  K+N EIVK QR++    + QLK+++E +  KT S K   IL  W  YL  F+Q+VPPSE+
Sbjct: 1431 IVVLGTFGRNIGAGMTGGIAYFLDEDNTLKNKLNTEIVKAQRLLTKESEEQLKNIMELYEIKTKSEKAKLILDNWSQYLAKFYQIVPPSEQ 1521          
BLAST of EY734994 vs. ExPASy Swiss-Prot
Match: GLTB_SYNY3 (Ferredoxin-dependent glutamate synthase 1 OS=Synechocystis sp. (strain PCC 6803) GN=gltB PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 8.087e-15
Identity = 42/109 (38.53%), Postives = 63/109 (57.80%), Query Frame = 1
Query:    4 VVVLGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKVQRVIAPVGQMQLKSLIEAHVEKTGSSKGSAILKEWDTYLPLFWQLVPPSEEDTPQACARVCKNSYWGGD 330
            VVVLG+ GRN AAGM+GG+AYI DE      + N  +V ++++  P     LK LI+ HV  T S+KG A+L +W+  +P F +++P   +   QA  +  +    G D
Sbjct: 1423 VVVLGQTGRNFAAGMSGGVAYIFDETGDFATRCNSAMVGLEKLEDPEEIKDLKELIQNHVNYTDSAKGKAVLADWEASIPKFVKVMPRDYKRVLQAIKKALEAGLSGDD 1531          
The following BLAST results are available for this feature:
BLAST of EY734994 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
GLTB_SPIOL1.785e-4689.90Ferredoxin-dependent glutamate synthase, chloropla... [more]
GLTB1_ARATH2.506e-4680.00Ferredoxin-dependent glutamate synthase 1, chlorop... [more]
GLTB_MAIZE3.722e-4485.86Ferredoxin-dependent glutamate synthase, chloropla... [more]
GLTB2_ARATH4.549e-4282.47Ferredoxin-dependent glutamate synthase 2, chlorop... [more]
GLTB_PORYE5.768e-2966.67Ferredoxin-dependent glutamate synthase OS=Porphyr... [more]
GLTB_PORPU1.285e-2859.62Ferredoxin-dependent glutamate synthase OS=Porphyr... [more]
GLTS_SYNY32.863e-2857.52Ferredoxin-dependent glutamate synthase 2 OS=Synec... [more]
GLTB_CYACA1.470e-2456.84Ferredoxin-dependent glutamate synthase OS=Cyanidi... [more]
GLTB_ANTSP3.390e-2153.85Ferredoxin-dependent glutamate synthase OS=Antitha... [more]
GLTB_SYNY38.087e-1538.53Ferredoxin-dependent glutamate synthase 1 OS=Synec... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-075-B03-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY734994 ID=EY734994; Name=EY734994; organism=Citrus sinensis; type=EST; length=738bp
TGTGTCGTTGTACTTGGAAAAGTGGGTAGAAATGTTGCTGCTGGAATGAC
TGGAGGTTTGGCATACATTCTCGATGAGGATGATACTCTCATCCCCAAGG
TCAATAAGGAGATAGTGAAGGTCCAGAGGGTGATTGCTCCTGTTGGCCAG
ATGCAGTTGAAGAGCCTTATTGAAGCCCATGTTGAAAAAACTGGCAGCAG
CAAAGGCTCTGCTATTCTCAAGGAATGGGATACGTATCTACCGCTCTTCT
GGCAGCTGGTTCCACCTAGCGAAGAAGACACCCCACAGGCTTGTGCCAGA
GTATGTAAGAACAGCTACTGGGGAGGTGACATTGCAGTCACCTTACGACA
ACAATTTGACATTATACGATGGCCTCGAAGCCTGCATGCAAAGCCAGAAA
ATTCCAATCACACCTTGTACAATTCAAACAGAGAGATTGATTTTGAAATA
GGAAATTCTCCATCCATGAAAGGGAGACTCCAATTTCACCATGAAAGCAA
CTTTGAATATTTATTGGCAAAGAAACATCATATCTGGATTGATCTGCTGG
GGTAGAAATTTCTTCCTCGATATTACCGGACTTGTATACCGAAATTGAGG
CCGACTGTAATGGAAGTTTAGGTTTTTCCCATCTTCCCAGGCTAAATTAA
GGGTTATGTAGAAATTGTGAACCCTGGATACACTTCTTAGCACCGGGCCT
TGCCCTCGTTTGTTCCTTGCCTGCTCTTCTCCCCCAAC
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