Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange

Publication Overview
TitleCharacterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange
AuthorsHuerta L, Garcia-Lor A, Garcia-Martinez JL
TypeJournal Article
Journal NameTree physiology
Volume29
Issue4
Year2009
Page(s)569-577
CitationHuerta L, Garcia-Lor A, Garcia-Martinez JL. Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange. Tree physiology. 2009; 29(4):569-577.

Abstract

CcGA20ox1 is a gene encoding a GA 20-oxidase, a gibberellin (GA) biosynthetic enzyme, previously isolated from the citrus hybrid Carrizo citrange (Citrus sinensis (L.) Osbeck x Poncirus trifoliata (L.) Raf.). Southern blot analysis of genomic DNA of Carrizo citrange with CcGA20ox1 suggested the presence in the hybrid of another gene encoding another GA 20-oxidase. A cDNA clone from this new gene (CcGA20ox2) was isolated using RNA from the other parent C. sinensis. CcGA20ox2 encoded a protein of 372 amino acids that showed 67.1% identity with CcGA20ox1, and its expression product catalyzed the in vitro conversion of GA12 to GA9, confirming that it corresponds to another active GA20ox. Amplification of genomic DNA and isolation of genomic clones of CcGA20ox1 and CcGA20ox2 revealed that the parental sources of these genes in the hybrid were P. trifoliata and C. sinensis, respectively. The sequences of CcGA20ox1 and CcGA20ox2 showed that both genes contained two introns, which are also conserved in GA20ox genes of other species like Arabidopsis thaliana L., Pisum sativum L. and Solanum lycopersicum L. Determination of transcript levels in the Carrizo citrange hybrid by quantitative real-time polymerase chain reaction showed that CcGA20ox1 was expressed mainly in internodes, leaves and seeds, and CcGA20ox2 in flower buds and flowers at anthesis, with the genes having similar transcript levels in young developing fruits.
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Carrizo CitrangeCarrizo Citrangebreeding_research_material
Washington NavelWashington Navelcultivar
Trifoliate orangeTrifoliate orangecultivar
Features
This publication contains information about 6 features:
Feature NameUniquenameType
CcGA20ox1-geneCcGA20ox1-genegenetic_marker
CcGA20ox1-PCRCcGA20ox1-PCRgenetic_marker
CcGA20ox2-geneCcGA20ox2-genegenetic_marker
CcGA20ox2-PCRCcGA20ox2-PCRgenetic_marker
EU834067EU834067.1region
EU834066EU834066.1region
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2009
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1093/treephys/tpn049
KeywordsCitroncirus webberi, enzymes, gibberellins, genes, gene expression, rootstocks, gene expression regulation, enzyme activity, polymerase chain reaction, sequence analysis, transcription (genetics), biosynthesis, GA 20-oxidase
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4191788
PMID: PubMedPMID:19203975