DN617540

Overview
NameDN617540
Unique NameDN617540
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length731
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DN617540_ssr107 microsatellite DN617540_ssr107:107..118. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9442 contig Csv1_Contig9442:9..739. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_AEDAE (Mediator of RNA polymerase II transcription subunit 10 OS=Aedes aegypti GN=MED10 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.010e-18
Identity = 45/129 (34.88%), Postives = 77/129 (59.69%), Query Frame = 1 
Query:  250 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 636
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL++TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQVDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELDETFPTEIAKYKAIRGD 129
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_ANOGA (Mediator of RNA polymerase II transcription subunit 10 OS=Anopheles gambiae GN=MED10 PE=3 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 4.477e-18
Identity = 45/129 (34.88%), Postives = 76/129 (58.91%), Query Frame = 1 
Query:  250 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 636
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL +TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQIDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELSETFPVEISKYKAIRGD 129
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_MOUSE (Mediator of RNA polymerase II transcription subunit 10 OS=Mus musculus GN=Med10 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 9.335e-16
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLSQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_PONAB (Mediator of RNA polymerase II transcription subunit 10 OS=Pongo abelii GN=MED10 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.219e-15
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_HUMAN (Mediator of RNA polymerase II transcription subunit 10 OS=Homo sapiens GN=MED10 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.219e-15
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_BOVIN (Mediator of RNA polymerase II transcription subunit 10 OS=Bos taurus GN=MED10 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.219e-15
Identity = 43/117 (36.75%), Postives = 70/117 (59.83%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_XENTR (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus tropicalis GN=med10 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.592e-15
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN LV+ L ++ K  ++ + I VP EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVTGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_XENLA (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus laevis GN=med10 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.716e-15
Identity = 42/126 (33.33%), Postives = 74/126 (58.73%), Query Frame = 1 
Query:  256 QNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            +  + + + ++K +  + QL + VS F  +SQ  L Q+LN LV  L ++ K  ++ + I VP EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ +R
Sbjct:    3 EKFDNLEDHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVRGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAVR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_MACFA (Mediator of RNA polymerase II transcription subunit 10 OS=Macaca fascicularis GN=MED10 PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 7.903e-15
Identity = 42/117 (35.90%), Postives = 69/117 (58.97%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 630
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+  L + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQGLSKVFPEDMAKYRSIR 128
[Close]
BLAST of DN617540 vs. ExPASy Swiss-Prot
Match: MED10_DANRE (Mediator of RNA polymerase II transcription subunit 10 OS=Danio rerio GN=med10 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.003e-14
Identity = 40/119 (33.61%), Postives = 69/119 (57.98%), Query Frame = 1 
Query:  283 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPTEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 636
            ++K +  + QL + VS F  +SQ  L Q+LN +++ L ++ K  ++ + I VP EV   ID G+NP  +T++ +   +AKN+  KGK D     +  L+ EL + FP+E+  Y+ I  +
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQTGLNQKLNFMITGLQDIEKCRQQLHDINVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLTKFKSLLISELGKVFPEEMAKYKAIHGD 130
The following BLAST results are available for this feature:
BLAST of DN617540 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
MED10_AEDAE2.010e-1834.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_ANOGA4.477e-1834.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_MOUSE9.335e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_PONAB1.219e-1536.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_HUMAN1.219e-1536.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_BOVIN1.219e-1536.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENTR1.592e-1536.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENLA2.716e-1533.33Mediator of RNA polymerase II transcription subuni... [more]
MED10_MACFA7.903e-1535.90Mediator of RNA polymerase II transcription subuni... [more]
MED10_DANRE3.003e-1433.61Mediator of RNA polymerase II transcription subuni... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS11_01F15_r Parent Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0001F15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DN617540 ID=DN617540; Name=DN617540; organism=Citrus sinensis; type=EST; length=731bp
CTAAACAGCGATTCGCAGAAGTTTTGGGGTCCTGAAGTTTCAACCCAATC
GATGATTACTTGAATATTGACTAGAAAGAAAATAAACAGGGCAAAATTCG
GACTCTGAAGAAGAAGAAAACTGTAATTACAGTTTCTTGATACGAGAGAA
AGAAAGATGGATGGACCGGTGGGGGGCAGCAGAGCAAGTGGTGGCAATGG
AATGGTGAGCAATCAAGCAAACGATACAACAACTGTGGCAGCTGACGATC
CGAAGCAGAACTTGAACCAGGTTATTAATTCAGTTCAGAAGACTCTTGGG
CTCCTACATCAACTCTACCTTACCGTATCTTCCTTCAATGCTGCCTCCCA
GCTCCCTCTCCTACAGAGACTCAATTCTCTTGTTTCGGAGCTTGACAACA
TGGTTAAATTGTCTGAGAAGTGCAATATTCAAGTGCCCACGGAAGTGCTT
AATTTGATTGATGACGGAAAAAATCCTGATGAATTCACAAGGGATGTTAT
AAACAGCTGCATTGCTAAAAACCAAGTCACCAAAGGAAAAACTGATGCTT
TCAAGAGTTTACGAAAGCATCTTTTGGACGAACTTGAGCAGACATTTCCA
GATGAAGTTGAAGCATATAGAGAGATACGTGCAAATTCTGCTGCTGAAGC
AAAGCGTCTTGCTCAATCACAAAGTATGTTACCAAATGGAGATGTGAAAG
TTAAATCTGAGCTTTAAATGTCAGTTTCATG
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