DC887651

Overview
NameDC887651
Unique NameDC887651
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length483
Libraries
Library NameType
EICcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4536 contig Csv1_Contig4536:288..769. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL321_ARATH (60S ribosomal protein L32-1 OS=Arabidopsis thaliana GN=RPL32A PE=2 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 9.743e-44
Identity = 84/94 (89.36%), Postives = 90/94 (95.74%), Query Frame = -2 
Query:  162 DSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 443
            DSRVRRKFKG TLMPN+G GSDKKTRHYLPNGFKKFVVHNT ELELLMMHNRTYCAEIAH+VST+KRK IVERA+QLD+VVTN+LARLRSQEDE
Sbjct:   40 DSRVRRKFKGVTLMPNVGYGSDKKTRHYLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAIVERASQLDVVVTNRLARLRSQEDE 133
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL322_ARATH (60S ribosomal protein L32-2 OS=Arabidopsis thaliana GN=RPL32B PE=2 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 6.315e-43
Identity = 81/94 (86.17%), Postives = 89/94 (94.68%), Query Frame = -2 
Query:  162 DSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 443
            DSRVRRKFKG TLMPN+G GSDKKTRHYLPNGFKKF+VHNT ELELLMMHNRTYCAEIAH++ST+KRK IVERA+QLD+VV+NKL RLRSQEDE
Sbjct:   40 DSRVRRKFKGVTLMPNVGYGSDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVVVSNKLGRLRSQEDE 133
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_SPOFR (60S ribosomal protein L32 OS=Spodoptera frugiperda GN=RpL32 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 1.098e-33
Identity = 70/97 (72.16%), Postives = 80/97 (82.47%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKTRH LPNGF+K +VHN +ELE+LMM NR YCAEIAH VS++KRK IVERA QL I VTN  ARLRSQE+E
Sbjct:   38 RGIDNRVRRRFKGQYLMPNIGYGSNKKTRHMLPNGFRKVLVHNVRELEILMMQNRKYCAEIAHGVSSKKRKTIVERAQQLSIRVTNSAARLRSQENE 134

HSP 2 Score: 21.9422 bits (45), Expect = 1.098e-33
Identity = 6/10 (60.00%), Postives = 9/10 (90.00%), Query Frame = -1 
Query:  448 VKENWRRPKG 477
            +K NWR+P+G
Sbjct:   30 LKRNWRKPRG 39
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_RAT (60S ribosomal protein L32 OS=Rattus norvegicus GN=Rpl32 PE=1 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 5.361e-33
Identity = 67/97 (69.07%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 5.361e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_PIG (60S ribosomal protein L32 OS=Sus scrofa GN=RPL32 PE=2 SV=3)

HSP 1 Score: 136.732 bits (343), Expect = 5.361e-33
Identity = 67/97 (69.07%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 5.361e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_MOUSE (60S ribosomal protein L32 OS=Mus musculus GN=Rpl32 PE=2 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 5.361e-33
Identity = 67/97 (69.07%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 5.361e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_MACFA (60S ribosomal protein L32 OS=Macaca fascicularis GN=RPL32 PE=2 SV=3)

HSP 1 Score: 136.732 bits (343), Expect = 5.361e-33
Identity = 67/97 (69.07%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 5.361e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_HUMAN (60S ribosomal protein L32 OS=Homo sapiens GN=RPL32 PE=1 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 5.361e-33
Identity = 67/97 (69.07%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 5.361e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_BOVIN (60S ribosomal protein L32 OS=Bos taurus GN=RPL32 PE=2 SV=3)

HSP 1 Score: 136.346 bits (342), Expect = 6.982e-33
Identity = 66/97 (68.04%), Postives = 83/97 (85.57%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVRR+FKG  LMPNIG GS+KKT+H LP+GF+KF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I +TN  ARLRS+E+E
Sbjct:   39 RGIDNRVRRRFKGQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRITNPNARLRSEENE 135

HSP 2 Score: 23.8682 bits (50), Expect = 6.982e-33
Identity = 6/12 (50.00%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + +K NWR+P+G
Sbjct:   29 VKIKRNWRKPRG 40
[Close]
BLAST of DC887651 vs. ExPASy Swiss-Prot
Match: RL32_ICTPU (60S ribosomal protein L32 OS=Ictalurus punctatus GN=rpl32 PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 4.438e-32
Identity = 67/97 (69.07%), Postives = 81/97 (83.51%), Query Frame = -2 
Query:  162 RVXDSRVRRKFKGCTLMPNIGXGSDKKTRHYLPNGFKKFVVHNTKELELLMMHNRTYCAEIAHDVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE 452
            R  D+RVR  FKG  LMPNIG GS+KKT+H LP+GFKKF+VHN KELE+L+M N++YCAEIAH+VS++ RK IVERAAQL I VTN  ARLRS+E+E
Sbjct:   39 RGIDNRVRSGFKGQMLMPNIGYGSNKKTKHMLPSGFKKFLVHNVKELEVLLMSNKSYCAEIAHNVSSKNRKVIVERAAQLAIKVTNPNARLRSEENE 135

HSP 2 Score: 22.7126 bits (47), Expect = 4.438e-32
Identity = 5/12 (41.67%), Postives = 10/12 (83.33%), Query Frame = -1 
Query:  448 ISVKENWRRPKG 483
            + ++ NWR+P+G
Sbjct:   29 VKIRRNWRKPRG 40
The following BLAST results are available for this feature:
BLAST of DC887651 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 36
Match NameE-valueIdentityDescription
RL321_ARATH9.743e-4489.3660S ribosomal protein L32-1 OS=Arabidopsis thalian... [more]
RL322_ARATH6.315e-4386.1760S ribosomal protein L32-2 OS=Arabidopsis thalian... [more]
RL32_SPOFR1.098e-3372.1660S ribosomal protein L32 OS=Spodoptera frugiperda... [more]
RL32_RAT5.361e-3369.0760S ribosomal protein L32 OS=Rattus norvegicus GN=... [more]
RL32_PIG5.361e-3369.0760S ribosomal protein L32 OS=Sus scrofa GN=RPL32 P... [more]
RL32_MOUSE5.361e-3369.0760S ribosomal protein L32 OS=Mus musculus GN=Rpl32... [more]
RL32_MACFA5.361e-3369.0760S ribosomal protein L32 OS=Macaca fascicularis G... [more]
RL32_HUMAN5.361e-3369.0760S ribosomal protein L32 OS=Homo sapiens GN=RPL32... [more]
RL32_BOVIN6.982e-3368.0460S ribosomal protein L32 OS=Bos taurus GN=RPL32 P... [more]
RL32_ICTPU4.438e-3269.0760S ribosomal protein L32 OS=Ictalurus punctatus G... [more]

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Properties
Property NameValue
Genbank descriptionDC887651 EIC Citrus sinensis cDNA clone EIC0679 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC887651 ID=DC887651; Name=DC887651; organism=Citrus sinensis; type=EST; length=483bp
CATATATTTTTATGAATAAAAACTACTGTATTATCACTCATCCCAAAAAA
AATAAAAAATCACAAACGGTCGTATCTTTGTAAGTCAAAATTTTCAAAAT
TTTGATAAAGCGTACAAAACAAGACTCACTACTACTAAAAAGAACAGATT
GTTTAATCTCATTCATCTTCCTGGCTTCTCAATCTAGCAAGCTTGTTAGT
CACAACAATATCAAGTTGGGCAGCTCGCTCCACAATCTCTTTCCTCTTCC
TCGTGGAAACATCATGGGCAATTTCAGCACAGTAGGTCCTGTTGTGCATC
ATCAACAATTCAAGCTCTTTCGTATTGTGCACCACAAATTTCTTGAAACC
ATTTGGAAGATAGTGGCGTGTCTTCTTGTCCGAACCANAACCAATGTTGG
GCATTAGAGTGCATCCCTTGAACTTTCTTCGCACCCGGGAATCAANTACC
CTTTGGTCTGCGCCAATTTTCCTTGACGGAGAT
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