CN182374 | Citrus Genome Database

Overview


Name	CN182374
Unique Name	CN182374
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	533

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: ARA3_ARATH (Ras-related protein ARA-3 OS=Arabidopsis thaliana GN=ARA-3 PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 7.061e-56
Identity = 102/109 (93.58%), Postives = 105/109 (96.33%), Query Frame = -2

Query:  149 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADADSRAEPQTIKINQPD 475
            ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPT+KGQALADEYGIKFFETSAKTNLNVEEVFFSI RDIKQRL+D DSRAEP TIKI+Q D
Sbjct:   91 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTAKGQALADEYGIKFFETSAKTNLNVEEVFFSIGRDIKQRLSDTDSRAEPATIKISQTD 199

HSP 2 Score: 33.113 bits (74), Expect = 7.061e-56
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   77 FRTITTAYYRGAMG 90

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RB1BV_BETVU (Ras-related protein RAB1BV OS=Beta vulgaris GN=RAB1BV PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.623e-52
Identity = 96/101 (95.05%), Postives = 99/101 (98.02%), Query Frame = -2

Query:  173 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADADSRAEPQ 475
            ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPT+KGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLAD+D+R E Q
Sbjct:   91 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTAKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADSDTRQEAQ 191

HSP 2 Score: 33.113 bits (74), Expect = 2.623e-52
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   77 FRTITTAYYRGAMG 90

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8A_DICDI (Ras-related protein Rab-8A OS=Dictyostelium discoideum GN=rab8A PE=3 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 8.798e-37
Identity = 68/104 (65.38%), Postives = 87/104 (83.65%), Query Frame = -2

Query:  164 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADADSRAEPQTIK 475
            ILLVYDVTDE SF NIRNWIRNIEQHA+D+VNK+L+GNK DM E K+ V +S+G++LADEYGIKF ETSAK ++NVEE F S+A+DIK+R+ D  +  +PQ ++
Sbjct:   91 ILLVYDVTDEKSFGNIRNWIRNIEQHATDSVNKMLIGNKCDMAE-KKVVDSSRGKSLADEYGIKFLETSAKNSINVEEAFISLAKDIKKRMIDTPNE-QPQVVQ 192

HSP 2 Score: 33.113 bits (74), Expect = 8.798e-37
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   77 FRTITTAYYRGAMG 90

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8B_DICDI (Ras-related protein Rab-8B OS=Dictyostelium discoideum GN=rab8B PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 5.604e-36
Identity = 69/110 (62.73%), Postives = 84/110 (76.36%), Query Frame = -2

Query:  146 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADADSRAEPQTIKINQPDH 475
            ILLVYDVTDE SF +IRNWIRNIEQHASD+VNK+L+GNK DM E K+ V +S+G++LADEYGIKF ETSAK ++NVEE F  +A+DIK+R+ D            N PDH
Sbjct:   91 ILLVYDVTDEKSFGSIRNWIRNIEQHASDSVNKMLIGNKCDMTE-KKVVDSSRGKSLADEYGIKFLETSAKNSVNVEEAFIGLAKDIKKRMIDTP----------NDPDH 189

HSP 2 Score: 33.113 bits (74), Expect = 5.604e-36
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   77 FRTITTAYYRGAMG 90

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: YPTV2_VOLCA (GTP-binding protein yptV2 OS=Volvox carteri GN=YPTV2 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 6.042e-35
Identity = 61/91 (67.03%), Postives = 79/91 (86.81%), Query Frame = -2

Query:  203 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRL 475
            I+LVYD+TDE+SFNN+RNW+RNIEQHASDNVNKILVGNK D+ E KR V  ++GQALADE+G +F+ETSAK N++VEE F ++A+D+  R+
Sbjct:   89 IILVYDITDEASFNNVRNWMRNIEQHASDNVNKILVGNKLDLAEDKRVVSIARGQALADEFGFRFYETSAKDNVHVEEAFIAVAKDVLARM 179

HSP 2 Score: 30.0314 bits (66), Expect = 6.042e-35
Identity = 13/16 (81.25%), Postives = 14/16 (87.50%), Query Frame = -1

Query:  471 FRTITTAYYRGAMGNI 518
            FRTIT+AYYRGA G I
Sbjct:   75 FRTITSAYYRGAQGII 90

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: YPT2_SCHPO (GTP-binding protein ypt2 OS=Schizosaccharomyces pombe GN=ypt2 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 7.592e-32
Identity = 59/106 (55.66%), Postives = 80/106 (75.47%), Query Frame = -2

Query:  158 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADADSRAEPQTIKIN 475
            ILL+YDVTD+ SF+N+R W  N+EQHAS+NV KIL+GNK D  E +R V   +GQALADE G+KF E SAKTN+NV+E FF++AR+IK++  DA++    Q   ++
Sbjct:   85 ILLLYDVTDKKSFDNVRTWFSNVEQHASENVYKILIGNKCDC-EDQRQVSFEQGQALADELGVKFLEASAKTNVNVDEAFFTLAREIKKQKIDAENEFSNQANNVD 189

HSP 2 Score: 33.113 bits (74), Expect = 7.592e-32
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   71 FRTITTAYYRGAMG 84

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8A_RAT (Ras-related protein Rab-8A OS=Rattus norvegicus GN=Rab8a PE=2 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 3.059e-30
Identity = 55/91 (60.44%), Postives = 75/91 (82.42%), Query Frame = -2

Query:  203 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRL 475
            I+LVYD+T+E SF+NIRNWIRNIE+HAS +V K+++GNK D+++ KR V   +G+ LA +YGIKF ETSAK N+NVE  FF++ARDIK ++
Sbjct:   84 IMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVND-KRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKM 173

HSP 2 Score: 33.113 bits (74), Expect = 3.059e-30
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   70 FRTITTAYYRGAMG 83

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8A_MOUSE (Ras-related protein Rab-8A OS=Mus musculus GN=Rab8a PE=1 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 3.059e-30
Identity = 55/91 (60.44%), Postives = 75/91 (82.42%), Query Frame = -2

Query:  203 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRL 475
            I+LVYD+T+E SF+NIRNWIRNIE+HAS +V K+++GNK D+++ KR V   +G+ LA +YGIKF ETSAK N+NVE  FF++ARDIK ++
Sbjct:   84 IMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVND-KRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKM 173

HSP 2 Score: 33.113 bits (74), Expect = 3.059e-30
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   70 FRTITTAYYRGAMG 83

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8A_MACFA (Ras-related protein Rab-8A OS=Macaca fascicularis GN=RAB8A PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.059e-30
Identity = 55/91 (60.44%), Postives = 75/91 (82.42%), Query Frame = -2

Query:  203 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRL 475
            I+LVYD+T+E SF+NIRNWIRNIE+HAS +V K+++GNK D+++ KR V   +G+ LA +YGIKF ETSAK N+NVE  FF++ARDIK ++
Sbjct:   84 IMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVND-KRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKM 173

HSP 2 Score: 33.113 bits (74), Expect = 3.059e-30
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   70 FRTITTAYYRGAMG 83

[Close]

BLAST of CN182374 vs. ExPASy Swiss-Prot
Match: RAB8A_HUMAN (Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8A PE=1 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.059e-30
Identity = 55/91 (60.44%), Postives = 75/91 (82.42%), Query Frame = -2

Query:  203 ILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRL 475
            I+LVYD+T+E SF+NIRNWIRNIE+HAS +V K+++GNK D+++ KR V   +G+ LA +YGIKF ETSAK N+NVE  FF++ARDIK ++
Sbjct:   84 IMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVND-KRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKM 173

HSP 2 Score: 33.113 bits (74), Expect = 3.059e-30
Identity = 14/14 (100.00%), Postives = 14/14 (100.00%), Query Frame = -1

Query:  477 FRTITTAYYRGAMG 518
            FRTITTAYYRGAMG
Sbjct:   70 FRTITTAYYRGAMG 83

The following BLAST results are available for this feature:

BLAST of CN182374 vs. ExPASy Swiss-Prot

BLAST of CN182374 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 307


Match Name	E-value	Identity	Description
ARA3_ARATH	7.061e-56	93.58	Ras-related protein ARA-3 OS=Arabidopsis thaliana ...	[more]
RB1BV_BETVU	2.623e-52	95.05	Ras-related protein RAB1BV OS=Beta vulgaris GN=RAB...	[more]
RAB8A_DICDI	8.798e-37	65.38	Ras-related protein Rab-8A OS=Dictyostelium discoi...	[more]
RAB8B_DICDI	5.604e-36	62.73	Ras-related protein Rab-8B OS=Dictyostelium discoi...	[more]
YPTV2_VOLCA	6.042e-35	67.03	GTP-binding protein yptV2 OS=Volvox carteri GN=YPT...	[more]
YPT2_SCHPO	7.592e-32	55.66	GTP-binding protein ypt2 OS=Schizosaccharomyces po...	[more]
RAB8A_RAT	3.059e-30	60.44	Ras-related protein Rab-8A OS=Rattus norvegicus GN...	[more]
RAB8A_MOUSE	3.059e-30	60.44	Ras-related protein Rab-8A OS=Mus musculus GN=Rab8...	[more]
RAB8A_MACFA	3.059e-30	60.44	Ras-related protein Rab-8A OS=Macaca fascicularis ...	[more]
RAB8A_HUMAN	3.059e-30	60.44	Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS04_0002F18_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0002F18, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN182374 ID=CN182374; Name=CN182374; organism=Citrus sinensis; type=EST; length=533bp
TACAAGCACCAATGATATAATTGACAAAATTAGTCGTCCCAACCAAAATG
ATTGCCTTGACATATACCCATGTGATCTCACCCTCATAGATTGCTTAAGA
ACCGCAGCAAGCTGATCTTTGGGCAGCTTGAGCAGCCCCACCTGCATGGT
CAGGTTGGTTAATTTTGATAGTCTGAGGCTCAGCCCTAGAGTCTGCATCT
GCAAGCCTTTGCTTGATGTCCCTTGCAATTGAAAAGAAAACCTCCTCCAC
ATTTAGGTTTGTCTTTGCACTAGTCTCAAAGAACTTGATGCCATATTCAT
CAGCAAGAGCTTGGCCCTTTGAAGTGGGGACAGCCCTTTTGCTTTCGTCC
ATGTCGGCCTTGTTACCCACCAGAATCTTGTTTACATTGTCAGATGCATG
CTGTTCGATATTACGGATCCAATTCCTAATGTTGTTGAAAGATGACTCGT
CGGTGACATCATATACGAGCAATATTTCCCATGGCTCCTCGGTAGTATGC
GGTTGTAATTGTTCGAAACCCTCTCTTGACCTG