CN181867

Overview
NameCN181867
Unique NameCN181867
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length678
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN181867_ssr126 microsatellite CN181867_ssr126:126..137. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig5079 contig Csv1_Contig5079:1..663. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=1 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 4.555e-61
Identity = 96/122 (78.69%), Postives = 104/122 (85.25%), Query Frame = -2 
Query:  243 ISIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGLS 608
            ISIH C L A PDL+ASKGS PTYLGRPWKLYSRVVYM++ MGDHI PRGWLEWNG FALD+LYYGEYMN G GS +G RV+WPGY VITS  EASKFTV+QFI GSSWLPSTGV+F  GLS
Sbjct:  465 ISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

HSP 2 Score: 46.2098 bits (108), Expect = 4.555e-61
Identity = 21/23 (91.30%), Postives = 21/23 (91.30%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KPMA QK TITAQNRKDPNQNTG
Sbjct:  442 KPMAQQKITITAQNRKDPNQNTG 464
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 8.336e-57
Identity = 87/123 (70.73%), Postives = 102/123 (82.93%), Query Frame = -2 
Query:  240 ISIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGLSV 608
            ISIH   + AA DLQA+ GS  TYLGRPWKL+SR VYM++Y+G H+  RGWLEWN  FALDTLYYGEY+NSGPGS +G RV WPGYRVI S  EA++FTV++FIYGSSWLPSTGV+FL GLS+
Sbjct:  476 ISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLSI 598

HSP 2 Score: 47.3654 bits (111), Expect = 8.336e-57
Identity = 21/23 (91.30%), Postives = 21/23 (91.30%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KPM  QKNTITAQNRKDPNQNTG
Sbjct:  453 KPMDFQKNTITAQNRKDPNQNTG 475
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 5.389e-43
Identity = 72/120 (60.00%), Postives = 90/120 (75.00%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            +I   ++ A  DL  +  +  TYLGRPWKLYSR V+M  YM D I P GWLEWNG+FALDTLYYGEYMNSGPG+++  RV+WPGY V+ ++ EA+ FTVSQ I G+ WLPSTG+ F+ GL
Sbjct:  406 TIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

HSP 2 Score: 33.4982 bits (75), Expect = 5.389e-43
Identity = 14/18 (77.78%), Postives = 16/18 (88.89%), Query Frame = -1 
Query:  610 QKNTITAQNRKDPNQNTG 663
            QKN+ITAQ RKDPN+ TG
Sbjct:  387 QKNSITAQGRKDPNEPTG 404
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.438e-42
Identity = 72/124 (58.06%), Postives = 93/124 (75.00%), Query Frame = -2 
Query:  240 ISIHDCSLYAAPDLQA-SKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGLSV 608
            ISI  C++ A  DL+A S  S PTYLGRPWKLYSR V M +++ + I+P GWLEWNGDFAL++L+YGEYMN GPG+ +G RV+WPGY+V   +T+A  +TV+QFI G+ WLPSTGV +     V
Sbjct:  399 ISIQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFGV 522

HSP 2 Score: 34.2686 bits (77), Expect = 3.438e-42
Identity = 15/23 (65.22%), Postives = 18/23 (78.26%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            K +  QKN+ITAQ RKDPN+ TG
Sbjct:  376 KGLPNQKNSITAQGRKDPNEPTG 398
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 3.723e-41
Identity = 69/125 (55.20%), Postives = 93/125 (74.40%), Query Frame = -2 
Query:  246 RTRAISIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 620
            ++  I IH+C + AA DL+  + S+ T+LGRPWK YSR VY+ T++   I P GW+EW+GDFAL+TLYY EYMN+GPGS+  +RV+W GY V+TS ++ S+FTV  FI G+SWLP+T V F  GL
Sbjct:  386 QSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

HSP 2 Score: 30.4166 bits (67), Expect = 3.723e-41
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  610 NTITAQNRKDPNQNTG 657
            NT+TAQ R DPNQ+TG
Sbjct:  374 NTLTAQGRTDPNQSTG 389
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME21_SOLLC (Pectinesterase 2.1 OS=Solanum lycopersicum GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 154.451 bits (389), Expect = 2.371e-40
Identity = 70/120 (58.33%), Postives = 84/120 (70.00%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            SI  C + A+PDL+      PTYLGRPWK YSR V M +Y+G  I P GW EW+GDFAL TLYYGE+MN+GPG+    RV+WPGY VIT   EA  FTV++ I G SWL ST VA++ GL
Sbjct:  429 SIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYLGGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAKLIQGGSWLRSTDVAYVDGL 548

HSP 2 Score: 32.3426 bits (72), Expect = 2.371e-40
Identity = 14/23 (60.87%), Postives = 16/23 (69.57%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KP   Q+N +TAQ R DPNQ TG
Sbjct:  405 KPGKYQQNMVTAQGRTDPNQATG 427
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME3_SOLLC (Pectinesterase 3 OS=Solanum lycopersicum GN=PME3 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.372e-40
Identity = 68/120 (56.67%), Postives = 82/120 (68.33%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            SI  C + A+PDL+       TYLGRPWK +SR V M +Y+  HI P GW EW GDFAL TLYYGE+MN+GPG+    RV+WPGY VIT   EA  FTV++ I G SWL ST VA++ GL
Sbjct:  423 SIQFCDIIASPDLEPVMNEYKTYLGRPWKKHSRTVVMQSYLDGHIDPSGWFEWRGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPNEAMPFTVAELIQGGSWLNSTSVAYVEGL 542

HSP 2 Score: 34.2686 bits (77), Expect = 2.372e-40
Identity = 15/23 (65.22%), Postives = 16/23 (69.57%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KP   QKN +TAQ R DPNQ TG
Sbjct:  399 KPNKRQKNMVTAQGRTDPNQATG 421
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME1_FICAW (Pectinesterase/pectinesterase inhibitor OS=Ficus awkeotsang PE=1 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.090e-40
Identity = 67/120 (55.83%), Postives = 84/120 (70.00%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            SI +C ++A+ DL+  + +  +YLGRPWK YSR V M +Y+ D I P GWLEW+ DFAL TL+YGEY N GPGS    RV+WPGY VITS   A +FTV++ I G SWL STGV +  GL
Sbjct:  424 SIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543

HSP 2 Score: 37.3502 bits (85), Expect = 3.090e-40
Identity = 17/23 (73.91%), Postives = 18/23 (78.26%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            K M  Q+NTITAQ R DPNQNTG
Sbjct:  400 KQMKGQENTITAQGRTDPNQNTG 422
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 8.903e-40
Identity = 69/120 (57.50%), Postives = 84/120 (70.00%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            SI  C++ A+ DL+      PTYLGRPWK YSR V M +Y+G  I P GW EW+GDFAL TLYYGE+MN+GPG+    RV+WPGY  IT   EA  FTV++ I G SWL STGVA++ GL
Sbjct:  429 SIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRSTGVAYVDGL 548

HSP 2 Score: 32.3426 bits (72), Expect = 8.903e-40
Identity = 14/23 (60.87%), Postives = 16/23 (69.57%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KP   Q+N +TAQ R DPNQ TG
Sbjct:  405 KPGKYQQNMVTAQGRTDPNQATG 427
[Close]
BLAST of CN181867 vs. ExPASy Swiss-Prot
Match: PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 152.525 bits (384), Expect = 8.904e-40
Identity = 69/120 (57.50%), Postives = 85/120 (70.83%), Query Frame = -2 
Query:  246 SIHDCSLYAAPDLQASKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSAVGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGL 605
            SI  C++ A+ DL+      PTYLGRPWK YSR V M +Y+G  I P GW EW+GDFAL TLYYGE+MN+GPG+    RV+WPGY VIT   +A  FTV++ I G SWL STGVA++ GL
Sbjct:  425 SIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRSTGVAYVDGL 544

HSP 2 Score: 32.3426 bits (72), Expect = 8.904e-40
Identity = 14/23 (60.87%), Postives = 16/23 (69.57%), Query Frame = -1 
Query:  610 KPMAMQKNTITAQNRKDPNQNTG 678
            KP   Q+N +TAQ R DPNQ TG
Sbjct:  401 KPGKYQQNMVTAQGRTDPNQATG 423
The following BLAST results are available for this feature:
BLAST of CN181867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 64
Match NameE-valueIdentityDescription
PME61_ARATH4.555e-6178.69Probable pectinesterase/pectinesterase inhibitor 6... [more]
PME34_ARATH8.336e-5770.73Probable pectinesterase/pectinesterase inhibitor 3... [more]
PME32_ARATH5.389e-4360.00Probable pectinesterase/pectinesterase inhibitor 3... [more]
PME_PRUPE3.438e-4258.06Pectinesterase/pectinesterase inhibitor PPE8B OS=P... [more]
PME2_CITSI3.723e-4155.20Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE... [more]
PME21_SOLLC2.371e-4058.33Pectinesterase 2.1 OS=Solanum lycopersicum GN=PME2... [more]
PME3_SOLLC2.372e-4056.67Pectinesterase 3 OS=Solanum lycopersicum GN=PME3 P... [more]
PME1_FICAW3.090e-4055.83Pectinesterase/pectinesterase inhibitor OS=Ficus a... [more]
PME22_SOLLC8.903e-4057.50Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2... [more]
PME1_SOLLC8.904e-4057.50Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001J14_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001J14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181867 ID=CN181867; Name=CN181867; organism=Citrus sinensis; type=EST; length=678bp
TTTTTTTTTTTTTTTTTCTTTTAAGACCGTCACAAATATTATTACTTATC
ACAACATCAAGACGAAAAAAATGAAACCAGAAAATTATACAATTGCGAAA
GAAATATTTAGAATTTAACCTCCCCGTGTGTGTGTGTGCATGCAGTAAGT
AAAATTCCATTTGATCCTTCCAACACTCATGAGACCCACCTATATATAAT
AAGTATACGTACTAAAAAGTTAAATTAAAATTCACTTTAAACCGAAAGCC
CACCCAAGAAAGCCACCCCAGTCGACGGCAGCCACGACGACCCGTAGATA
AACTGCGACACAGTGAATTTACTTGCCTCCGTCGCCGACGTTATAACCCG
GTAACCCGGCCACCTCACCCGGTGCCCCACGGCCGAACCCGGGCCGGAGT
TCATGTACTCGCCGTAGTATAAAGTATCCAGTGCAAAGTCTCCATTCCAT
TCAAGCCAGCCTCTGGGTTGGATGTGGTCACCCATGTACGTTAACATGTA
CACAACCCTCGAGTAGAGCTTCCACGGCCGGCCTAGGTACGTGGGGATGC
TGCCTTTCGAGGCCTGAAGATCGGGGGCGGCGTAAAGGCTGCAGTCGTGG
ATTGAGATGGCCCGTGTTCTGATTAGGGTCTTTCCTGTTCTGCGCCGTGA
TGGTGTTCTTCTGCATGGCCATCGGCTT
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