CK939819

Overview
NameCK939819
Unique NameCK939819
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length277
Libraries
Library NameType
Developing fruit flavedo at 165 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:338..614. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 7.138e-46
Identity = 88/91 (96.70%), Postives = 90/91 (98.90%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 276
            VIQIDEAALREGLPLRKSEHAFYLDWA HSFRITNCGVQD+TQIHTHMCYS+FNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA
Sbjct:  601 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 691
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 179.874 bits (455), Expect = 3.542e-45
Identity = 88/91 (96.70%), Postives = 89/91 (97.80%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 276
            VIQIDEAALREGLPLRKSEHAFYLDWA HSFRITN GVQDTTQIHTHMCYSNFNDIIHSII+MDADVITIENSRSDEKLLSVFREGVKYGA
Sbjct:  600 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLSVFREGVKYGA 690
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 6.042e-45
Identity = 88/92 (95.65%), Postives = 88/92 (95.65%), Query Frame = 1 
Query:    1 NVIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 276
            NVIQIDEAALREGLPLRKSEH FYL WA HSFRITN GVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA
Sbjct:  600 NVIQIDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 691
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 8.725e-44
Identity = 85/92 (92.39%), Postives = 88/92 (95.65%), Query Frame = 1 
Query:    1 NVIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGA 276
            NVIQIDEAALREGLPLRK+EHAFYLDWA HSFRITN  +QDTTQIHTHMCYSNFNDIIHSIIDMDADV+TIENSRS EKLLSVFREGVKYGA
Sbjct:  600 NVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSIIDMDADVMTIENSRSSEKLLSVFREGVKYGA 691
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_MARAV (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=metE PE=3 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.352e-28
Identity = 62/85 (72.94%), Postives = 70/85 (82.35%), Query Frame = 1 
Query:    1 NVIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFR 255
            N+IQIDEAALREGLPLR+S+ A YL+WA +SFRI   GV D+TQIHTHMCYS FNDII +I  MDADVITIE SRSD +LL  FR
Sbjct:  598 NIIQIDEAALREGLPLRQSDWATYLEWAINSFRIAANGVDDSTQIHTHMCYSEFNDIIEAIARMDADVITIETSRSDMELLDAFR 682
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.307e-28
Identity = 65/89 (73.03%), Postives = 70/89 (78.65%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 270
            VIQIDEAALREGLPLR+++   YL WA  SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY
Sbjct:  604 VIQIDEAALREGLPLRRAQWGEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DNFKY 691
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.307e-28
Identity = 63/83 (75.90%), Postives = 68/83 (81.93%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVF 252
            VIQIDEAALREGLPLR+++ + YL WA  SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F
Sbjct:  604 VIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF 686
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.307e-28
Identity = 63/83 (75.90%), Postives = 68/83 (81.93%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVF 252
            VIQIDEAALREGLPLR+++ + YL WA  SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F
Sbjct:  604 VIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF 686
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_BURCC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain MC0-3) GN=metE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.307e-28
Identity = 63/83 (75.90%), Postives = 68/83 (81.93%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVF 252
            VIQIDEAALREGLPLR+++ + YL WA  SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F
Sbjct:  604 VIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF 686
[Close]
BLAST of CK939819 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 2.307e-28
Identity = 63/83 (75.90%), Postives = 68/83 (81.93%), Query Frame = 1 
Query:    4 VIQIDEAALREGLPLRKSEHAFYLDWAGHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVF 252
            VIQIDEAALREGLPLR+++ + YL WA  SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F
Sbjct:  604 VIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF 686
The following BLAST results are available for this feature:
BLAST of CK939819 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_ARATH7.138e-4696.705-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SOLSC3.542e-4596.705-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR6.042e-4595.655-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CATRO8.725e-4492.395-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MARAV1.352e-2872.945-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS32.307e-2873.035-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ2.307e-2875.905-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH2.307e-2875.905-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCC2.307e-2875.905-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA2.307e-2875.905-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004745_A09 Developing fruit flavedo at 165 DAFB Citrus sinensis cDNA clone F1650002_IF_A09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK939819 ID=CK939819; Name=CK939819; organism=Citrus sinensis; type=EST; length=277bp
AACGTTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAG
GAAATCTGAGCATGCTTTCTACTTGGACTGGGCGGGCCACTCCTTCAGAA
TCACCAACTGTGGTGTCCAGGACACTACCCAGATCCACACCCACATGTGC
TACTCCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGA
TGTGATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCT
TCCGTGAGGGAGTCAAGTATGGTGCTG
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