CK939384

Overview
NameCK939384
Unique NameCK939384
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length791
Libraries
Library NameType
Developing fruit flavedo at 165 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CK939384_ssr82 microsatellite CK939384_ssr82:82..96. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1947 contig Csv1_Contig1947:13..803. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: AB13I_ARATH (Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplastic OS=Arabidopsis thaliana GN=TGD3 PE=1 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 6.984e-58
Identity = 97/129 (75.19%), Postives = 109/129 (84.50%), Query Frame = 1 
Query:  295 QRKVVCACVAPPRDLGTDGFPATKFNDSSKSENVNTLFEP-EDDGDVLIECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRAGLISDEEISGLRIGLV 678
            +RKV C C+APP++L  D   ATKF+  +KS          E+D DVLIECR+VYKSFGEKHIL+GVSFKIRHGEAVG+IGPSGTGKSTILKI+AGLLAPDKGEVYIRG+KRAGLISDEEISGLRIGLV
Sbjct:   40 RRKVSCCCIAPPQNLDND---ATKFDSLTKSGGGMCKERGLENDSDVLIECRDVYKSFGEKHILKGVSFKIRHGEAVGVIGPSGTGKSTILKIMAGLLAPDKGEVYIRGKKRAGLISDEEISGLRIGLV 165

HSP 2 Score: 60.8474 bits (146), Expect = 6.984e-58
Identity = 30/38 (78.95%), Postives = 33/38 (86.84%), Query Frame = 2 
Query:  677 YENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            YE SKM + QISELV + LAAVGLKGVE+RLPSELSGG
Sbjct:  186 YERSKMSENQISELVTQTLAAVGLKGVENRLPSELSGG 223
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: CYSA_SYNY3 (Sulfate/thiosulfate import ATP-binding protein cysA OS=Synechocystis sp. (strain PCC 6803) GN=cysA PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.002e-13
Identity = 26/58 (44.83%), Postives = 42/58 (72.41%), Query Frame = 1 
Query:  448 NVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGR 621
            NV K FG+   L+ ++ ++  G+ V ++GPSG+GKST+L+ IAGL  PD+G++ I G+
Sbjct:    7 NVSKQFGDFTALKDINLEVPDGKLVALLGPSGSGKSTLLRAIAGLEEPDQGQIIINGQ 64

HSP 2 Score: 37.3502 bits (85), Expect = 1.002e-13
Identity = 21/51 (41.18%), Postives = 32/51 (62.75%), Query Frame = 2 
Query:  638 LAMRRYLVFGLDWYENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            L +R+ + FGL   E  K P  +  E V+E L+ + L+G+ +R PS+LSGG
Sbjct:   89 LTIRQNIAFGL---EIRKHPPAKTKERVEELLSLIQLEGLGNRYPSQLSGG 136
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: POTA_CLOAB (Spermidine/putrescine import ATP-binding protein potA OS=Clostridium acetobutylicum GN=potA PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.302e-13
Identity = 25/60 (41.67%), Postives = 41/60 (68.33%), Query Frame = 1 
Query:  427 DVLIECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEV 606
            D+LIE +NV K +GE ++++ ++  +R  E +  +GPSG GK+T L +IAG   PD+G +
Sbjct:    4 DILIELKNVSKKYGENYVIKNLNLFVRRNEFLTFLGPSGCGKTTTLNMIAGFETPDEGNI 63

HSP 2 Score: 36.1946 bits (82), Expect = 1.302e-13
Identity = 22/56 (39.29%), Postives = 33/56 (58.93%), Query Frame = 2 
Query:  647 RRYLVFG-LDWYEN-------SKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            ++Y +F  ++ YEN        K+P++QI E V++ L+ V LKG E R    LSGG
Sbjct:   85 QKYALFSHMNVYENVAFGLRIKKLPEKQIREEVEKMLSLVDLKGFEKRSTDSLSGG 140
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: POTA_LAWIP (Spermidine/putrescine import ATP-binding protein potA OS=Lawsonia intracellularis (strain PHE/MN1-00) GN=potA PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.689e-13
Identity = 29/67 (43.28%), Postives = 44/67 (65.67%), Query Frame = 1 
Query:  433 LIECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRAG 633
            +IE +NV K F +   L  ++ +I +GE + ++GPSG GK+TIL+II+G   PD GE+ I G   +G
Sbjct:    7 IIELKNVSKIFEDTCALDNINLQIANGEFLTLLGPSGCGKTTILRIISGFETPDVGEILIAGVNVSG 73

HSP 2 Score: 37.7354 bits (86), Expect = 1.689e-13
Identity = 22/51 (43.14%), Postives = 30/51 (58.82%), Query Frame = 2 
Query:  638 LAMRRYLVFGLDWYENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            + +R  + FGL      K P E+++ELV E L  V L+   DR PS+LSGG
Sbjct:   94 MTVRDNVAFGL---RMQKCPREKVNELVFEALRMVHLENFADRRPSQLSGG 141
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: CYSA_THEEB (Sulfate/thiosulfate import ATP-binding protein cysA OS=Thermosynechococcus elongatus (strain BP-1) GN=cysA PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.694e-13
Identity = 28/62 (45.16%), Postives = 39/62 (62.90%), Query Frame = 1 
Query:  436 IECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGR 621
            I   NV KSFG    ++ V   I  G  V ++GPSG+GKST+L++IAGL  PD G + + G+
Sbjct:    3 ITIENVSKSFGSFQAVKQVDLDIASGSLVALLGPSGSGKSTLLRLIAGLEMPDTGRILLTGK 64

HSP 2 Score: 37.3502 bits (85), Expect = 1.694e-13
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 2 
Query:  638 LAMRRYLVFGLDWYENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            + +R+ + FGL   E  K+P  +I+  V E L  V L G+ DR PS+LSGG
Sbjct:   89 MTVRQNIAFGL---ELRKVPRAKINARVAELLELVQLTGLGDRYPSQLSGG 136
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: POTA_PSYCK (Spermidine/putrescine import ATP-binding protein potA OS=Psychrobacter cryohalolentis (strain K5) GN=potA PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 4.796e-13
Identity = 27/73 (36.99%), Postives = 49/73 (67.12%), Query Frame = 1 
Query:  430 VLIECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRAGLISDE 648
            VL++   + K++    +L+ ++  I+HGE + ++GPSG GK+T+L++IAG   P  G +Y+ G + AGL +D+
Sbjct:   20 VLLQLTGLKKTYDNTEVLKDINLDIKHGEFITLLGPSGCGKTTLLRLIAGFEQPSAGAIYLDGVQMAGLPADK 92

HSP 2 Score: 30.4166 bits (67), Expect = 4.796e-13
Identity = 15/34 (44.12%), Postives = 22/34 (64.71%), Query Frame = 2 
Query:  689 KMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            K+P  +I   V+E LA V L+ + +R P +LSGG
Sbjct:  122 KVPKVEIQTRVREMLAMVQLEHLANRRPQDLSGG 155
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: CYSA_ANASP (Sulfate/thiosulfate import ATP-binding protein cysA OS=Anabaena sp. (strain PCC 7120) GN=cysA PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 6.261e-13
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:  436 IECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGR 621
            I   NV K FG    +  V+ +I+ G+ V ++GPSG+GKST+L++IAGL  PD G + + G+
Sbjct:    3 IVVENVSKQFGSFRAVDQVNLEIQSGKLVALLGPSGSGKSTLLRLIAGLEKPDDGRIILTGK 64

HSP 2 Score: 36.5798 bits (83), Expect = 6.261e-13
Identity = 21/51 (41.18%), Postives = 30/51 (58.82%), Query Frame = 2 
Query:  638 LAMRRYLVFGLDWYENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            L +R+ + FGL   E  K P  ++   V++ L  V L G+ DR PS+LSGG
Sbjct:   89 LTVRQNIAFGL---EIRKAPANKVKGRVEQLLELVQLSGLGDRYPSQLSGG 136
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: CYSA_MYCPA (Sulfate/thiosulfate import ATP-binding protein cysA OS=Mycobacterium paratuberculosis GN=cysA PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 8.114e-13
Identity = 32/68 (47.06%), Postives = 42/68 (61.76%), Query Frame = 1 
Query:  418 DDGDVLIECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGR 621
            D GD+ I  R+ YK +G+   L  V F +  G    ++GPSG+GKST+L+ IAGL  PD G V I GR
Sbjct:    7 DRGDIAITVRDAYKRYGDFVALDHVDFVVPTGSLTALLGPSGSGKSTLLRTIAGLDQPDTGTVTIYGR 74

HSP 2 Score: 31.187 bits (69), Expect = 8.114e-13
Identity = 18/51 (35.29%), Postives = 27/51 (52.94%), Query Frame = 2 
Query:  638 LAMRRYLVFGLDWYENSKMPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            L +R  + +GL   +  K P  +I   V   L  VGL G + R P++L+GG
Sbjct:   99 LTVRDNVAYGL---KVRKRPKAEIKAKVDNLLEVVGLSGFQGRYPNQLAGG 146
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: CYSA_BRAJA (Sulfate/thiosulfate import ATP-binding protein cysA OS=Bradyrhizobium japonicum GN=cysA PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 8.127e-13
Identity = 32/61 (52.46%), Postives = 43/61 (70.49%), Query Frame = 1 
Query:  436 IECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRG 618
            IE RN+ K FG    L GV+ K+ +GE + ++GPSG+GK+T+L+IIAGL  PD GEV   G
Sbjct:    3 IEVRNLVKKFGSFAALDGVNLKVDNGELLALLGPSGSGKTTLLRIIAGLDWPDSGEVSFNG 63

HSP 2 Score: 28.1054 bits (61), Expect = 8.127e-13
Identity = 19/46 (41.30%), Postives = 25/46 (54.35%), Query Frame = 2 
Query:  656 LVFGLDWYENSKMPDE-QISELVKENLAAVGLKGVEDRLPSELSGG 790
            + FGL     +   +E +I   VKE L  V L  + DR PS+LSGG
Sbjct:   95 VAFGLRVQPRAVRKEEARIRARVKELLDLVQLDWLADRYPSQLSGG 140
[Close]
BLAST of CK939384 vs. ExPASy Swiss-Prot
Match: FBPC_ECOLI (Fe(3+) ions import ATP-binding protein fbpC OS=Escherichia coli (strain K12) GN=fbpC PE=3 SV=3)

HSP 1 Score: 62.003 bits (149), Expect = 1.055e-12
Identity = 26/61 (42.62%), Postives = 42/61 (68.85%), Query Frame = 1 
Query:  436 IECRNVYKSFGEKHILRGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRG 618
            +E RNV K FG   ++  ++  I  G+ V ++GPSG GK+TIL+++AGL  P +G+++I G
Sbjct:    7 VELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDG 67

HSP 2 Score: 32.3426 bits (72), Expect = 1.055e-12
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 2 
Query:  692 MPDEQISELVKENLAAVGLKGVEDRLPSELSGG 790
            +P  ++   VKE LA V L+G EDR   ++SGG
Sbjct:  108 VPRAELKARVKEALAMVDLEGFEDRFVDQISGG 140
The following BLAST results are available for this feature:
BLAST of CK939384 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 59
Match NameE-valueIdentityDescription
AB13I_ARATH6.984e-5875.19Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplast... [more]
CYSA_SYNY31.002e-1344.83Sulfate/thiosulfate import ATP-binding protein cys... [more]
POTA_CLOAB1.302e-1341.67Spermidine/putrescine import ATP-binding protein p... [more]
POTA_LAWIP1.689e-1343.28Spermidine/putrescine import ATP-binding protein p... [more]
CYSA_THEEB1.694e-1345.16Sulfate/thiosulfate import ATP-binding protein cys... [more]
POTA_PSYCK4.796e-1336.99Spermidine/putrescine import ATP-binding protein p... [more]
CYSA_ANASP6.261e-1343.55Sulfate/thiosulfate import ATP-binding protein cys... [more]
CYSA_MYCPA8.114e-1347.06Sulfate/thiosulfate import ATP-binding protein cys... [more]
CYSA_BRAJA8.127e-1352.46Sulfate/thiosulfate import ATP-binding protein cys... [more]
FBPC_ECOLI1.055e-1242.62Fe(3+) ions import ATP-binding protein fbpC OS=Esc... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004751_D03 Developing fruit flavedo at 165 DAFB Citrus sinensis cDNA clone F1650001_IIIF_D03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK939384 ID=CK939384; Name=CK939384; organism=Citrus sinensis; type=EST; length=791bp
GGGGAAATCACAAAATTATCAAAACCCAGTAGTTGAAATGCTTCGAAAAC
CACTTTTTATGTGAACTTCCATTGATTATTCTATTTTATTTTATTTTTTT
CATTTTGTGGTTTTTAGACCAAATATCAATCTGCGTCTGCTTTACAATTA
TAAACACCGAAAATGGTCTCTTTATCTTCATCTGGTTCACTGTTATTTCC
TCTCGCAACAAAAAGTGTTAGTTCTCGATTGTCTACACTTGCTAATTCGG
GCAAAAAGCCGATCAATTCGTTCTATTATAAGGCGAAAGAAGAACAGAGA
AAAGTCGTTTGTGCTTGTGTAGCGCCTCCAAGGGACTTGGGCACTGATGG
ATTCCCTGCCACCAAATTCAATGATTCATCTAAATCAGAGAATGTTAATA
CCTTATTCGAACCCGAGGATGATGGTGATGTTCTCATCGAGTGTCGGAAT
GTTTACAAGTCATTTGGGGAAAAGCATATATTGAGAGGTGTGAGCTTCAA
GATTAGACATGGTGAAGCTGTTGGTATAATAGGGCCTTCAGGCACTGGGA
AATCAACGATTTTAAAGATTATTGCTGGGCTTCTTGCTCCAGACAAGGGA
GAGGTTTACATTCGAGGTAGGAAGAGAGCTGGTTTGATTAGCGATGAGGA
GATATCTGGTCTTCGGATTGGATTGGTATGAAAATTCGAAGATGCCTGAT
GAACAAATTTCAGAGCTTGTGAAGGAAAATTTGGCTGCCGTTGGATTAAA
GGGAGTTGAGGATCGCTTACCATCTGAGTTGTCTGGTGGAA
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