CK939146

Overview
NameCK939146
Unique NameCK939146
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length738
Libraries
Library NameType
Developing fruit albedo at 165 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS31_ARATH (40S ribosomal protein S3-1 OS=Arabidopsis thaliana GN=RPS3A PE=2 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 4.908e-100
Identity = 163/173 (94.22%), Postives = 169/173 (97.69%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MATQISKKRKFVADGVF+AELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTS+VQKRFKFP +SVELYAEKVN+RGLCAIAQAESLRYKLLGGLAVRRACYGVLRF+MESGAKGCEVIVSGKLRA RAKSMKFKDGYMVSSGQP  EYID+AVR
Sbjct:    1 MATQISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSLVQKRFKFPVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKSMKFKDGYMVSSGQPTKEYIDAAVR 173

HSP 2 Score: 66.6254 bits (161), Expect = 4.908e-100
Identity = 30/39 (76.92%), Postives = 32/39 (82.05%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTIHS 736
            HVLLRQGVLGIKVKIM  WDP GK  P TPLPD+V IH+
Sbjct:  174 HVLLRQGVLGIKVKIMLDWDPTGKSGPKTPLPDVVIIHA 212
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS33_ARATH (40S ribosomal protein S3-3 OS=Arabidopsis thaliana GN=RPS3C PE=1 SV=1)

HSP 1 Score: 317.775 bits (813), Expect = 6.403e-100
Identity = 162/173 (93.64%), Postives = 169/173 (97.69%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MATQISKKRKFVADGVF+AELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTS+VQKRFKFP++SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRF+MESGAKGCEVIVSGKLRA RAKSMKFKDGYMVSSGQP  EYID+AVR
Sbjct:    1 MATQISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSLVQKRFKFPQDSVELYAEKVANRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKSMKFKDGYMVSSGQPTKEYIDAAVR 173

HSP 2 Score: 67.781 bits (164), Expect = 6.403e-100
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTIHS 736
            HVLLRQGVLG+KVKIM  WDP GKQ P TPLPD+V IH+
Sbjct:  174 HVLLRQGVLGLKVKIMLDWDPKGKQGPMTPLPDVVIIHT 212
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS32_ARATH (40S ribosomal protein S3-2 OS=Arabidopsis thaliana GN=RPS3B PE=1 SV=1)

HSP 1 Score: 318.931 bits (816), Expect = 3.157e-99
Identity = 163/173 (94.22%), Postives = 168/173 (97.11%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            M TQISKKRKFVADGVF+AELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTS+VQKRFKFP +SVELYAEKVN+RGLCAIAQAESLRYKLLGGLAVRRACYGVLRF+MESGAKGCEVIVSGKLRA RAKSMKFKDGYMVSSGQP  EYIDSAVR
Sbjct:    1 MTTQISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSLVQKRFKFPVDSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAARAKSMKFKDGYMVSSGQPTKEYIDSAVR 173

HSP 2 Score: 64.3142 bits (155), Expect = 3.157e-99
Identity = 29/39 (74.36%), Postives = 31/39 (79.49%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTIHS 736
            HVLLRQGVLGIKVK+M  WDP G   P TPLPD+V IHS
Sbjct:  174 HVLLRQGVLGIKVKVMLDWDPKGISGPKTPLPDVVIIHS 212
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS31_XENLA (40S ribosomal protein S3-A OS=Xenopus laevis GN=rps3-A PE=2 SV=2)

HSP 1 Score: 295.819 bits (756), Expect = 8.487e-91
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 59.3066 bits (142), Expect = 8.487e-91
Identity = 28/37 (75.68%), Postives = 30/37 (81.08%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP+GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_RAT (40S ribosomal protein S3 OS=Rattus norvegicus GN=Rps3 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 8.488e-91
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 59.3066 bits (142), Expect = 8.488e-91
Identity = 28/37 (75.68%), Postives = 30/37 (81.08%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP+GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_MOUSE (40S ribosomal protein S3 OS=Mus musculus GN=Rps3 PE=1 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 8.488e-91
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 59.3066 bits (142), Expect = 8.488e-91
Identity = 28/37 (75.68%), Postives = 30/37 (81.08%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP+GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_PIG (40S ribosomal protein S3 OS=Sus scrofa GN=RPS3 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 1.107e-90
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 58.9214 bits (141), Expect = 1.107e-90
Identity = 28/37 (75.68%), Postives = 29/37 (78.38%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_HUMAN (40S ribosomal protein S3 OS=Homo sapiens GN=RPS3 PE=1 SV=2)

HSP 1 Score: 295.819 bits (756), Expect = 1.107e-90
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 58.9214 bits (141), Expect = 1.107e-90
Identity = 28/37 (75.68%), Postives = 29/37 (78.38%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_BOVIN (40S ribosomal protein S3 OS=Bos taurus GN=RPS3 PE=2 SV=1)

HSP 1 Score: 295.819 bits (756), Expect = 1.107e-90
Identity = 152/173 (87.86%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 58.9214 bits (141), Expect = 1.107e-90
Identity = 28/37 (75.68%), Postives = 29/37 (78.38%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSI 210
[Close]
BLAST of CK939146 vs. ExPASy Swiss-Prot
Match: RS3_AMBME (40S ribosomal protein S3 OS=Ambystoma mexicanum GN=RPS3 PE=2 SV=1)

HSP 1 Score: 294.664 bits (753), Expect = 1.884e-90
Identity = 151/173 (87.28%), Postives = 159/173 (91.91%), Query Frame = 1 
Query:  100 MATQISKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNSRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMVSSGQPVNEYIDSAVR 618
            MA QISKKRKFVADG+F AELNE LTRELAEDGYSGVEVRVTP RTEIII ATRTQNVLGEKGRRIRELT+VVQKRF FPE SVELYAEKV +RGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESG+KGCEV+VSGKLR QRAKSMKF DG M+ SG PVN Y+D+AVR
Sbjct:    1 MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGSKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVR 173

HSP 2 Score: 59.3066 bits (142), Expect = 1.884e-90
Identity = 28/37 (75.68%), Postives = 30/37 (81.08%), Query Frame = 2 
Query:  620 HVLLRQGVLGIKVKIMXXWDPNGKQXPTTPLPDLVTI 730
            HVLLRQGVLGIKVKIM  WDP+GK  P  PLPD V+I
Sbjct:  174 HVLLRQGVLGIKVKIMLAWDPSGKIGPKKPLPDHVSI 210
The following BLAST results are available for this feature:
BLAST of CK939146 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 70
Match NameE-valueIdentityDescription
RS31_ARATH4.908e-10094.2240S ribosomal protein S3-1 OS=Arabidopsis thaliana... [more]
RS33_ARATH6.403e-10093.6440S ribosomal protein S3-3 OS=Arabidopsis thaliana... [more]
RS32_ARATH3.157e-9994.2240S ribosomal protein S3-2 OS=Arabidopsis thaliana... [more]
RS31_XENLA8.487e-9187.8640S ribosomal protein S3-A OS=Xenopus laevis GN=rp... [more]
RS3_RAT8.488e-9187.8640S ribosomal protein S3 OS=Rattus norvegicus GN=R... [more]
RS3_MOUSE8.488e-9187.8640S ribosomal protein S3 OS=Mus musculus GN=Rps3 P... [more]
RS3_PIG1.107e-9087.8640S ribosomal protein S3 OS=Sus scrofa GN=RPS3 PE=... [more]
RS3_HUMAN1.107e-9087.8640S ribosomal protein S3 OS=Homo sapiens GN=RPS3 P... [more]
RS3_BOVIN1.107e-9087.8640S ribosomal protein S3 OS=Bos taurus GN=RPS3 PE=... [more]
RS3_AMBME1.884e-9087.2840S ribosomal protein S3 OS=Ambystoma mexicanum GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004433_F06 Developing fruit albedo at 165 DAFB Citrus sinensis cDNA clone A1650003_IIF_F06 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK939146 ID=CK939146; Name=CK939146; organism=Citrus sinensis; type=EST; length=738bp
GGGGAAACCTAGGGTTTGCAGAGAGCTAGCTGCGCACACAAGCCACCTCA
TTGAGATCATCAGATCCCTAACTCTGTAATCGAACCAACAGAAAGCAAAA
TGGCAACTCAAATCAGCAAGAAAAGAAAGTTCGTGGCTGATGGCGTGTTT
TTCGCGGAGCTTAACGAGGTACTGACTAGAGAGCTTGCGGAGGACGGTTA
TTCTGGAGTAGAGGTCAGGGTAACTCCCATGCGTACTGAGATCATCATCC
GAGCCACTCGCACTCAGAACGTTCTTGGTGAGAAAGGGAGGAGAATTAGA
GAGCTGACATCAGTGGTGCAGAAAAGGTTCAAATTCCCGGAGAATAGCGT
GGAGCTGTACGCTGAGAAAGTCAACAGTAGAGGACTATGTGCCATTGCTC
AAGCCGAGTCTCTTCGCTACAAGCTCCTTGGTGGGCTTGCTGTTCGCAGG
GCTTGCTATGGTGTCTTGAGGTTCATTATGGAAAGTGGAGCCAAGGGATG
TGAGGTCATTGTCAGTGGAAAGTTGAGGGCACAACGTGCAAAATCAATGA
AGTTTAAGGATGGATATATGGTCTCTTCTGGTCAGCCAGTGAATGAATAT
ATTGACTCTGCTGTGAGGACATGTTCTTTTGAGACAGGGTGTACTTGGTA
TTAAGGTCAAGATCATGCNNNGTTGGGATCCTAATGGAAAGCAANNNCCT
ACTACCCCTCTACCTGATCTCGTAACAATCCACTCACC
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