CK937319

Overview
NameCK937319
Unique NameCK937319
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length697
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_SOLTU (Soluble inorganic pyrophosphatase OS=Solanum tuberosum GN=PPA PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 2.893e-74
Identity = 107/118 (90.68%), Postives = 114/118 (96.61%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDKK+GLI VDR+LYSSVVYP NYGFIPRTLCEDNDP+DVL++MQEPVLPGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHY DIK+LPPHRLAEIRRFFEDYK N
Sbjct:   57 GSKVKYELDKKTGLIKVDRILYSSVVYPQNYGFIPRTLCEDNDPMDVLVLMQEPVLPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYTDIKQLPPHRLAEIRRFFEDYKKN 174

HSP 2 Score: 68.1662 bits (165), Expect = 2.893e-74
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:  219 PPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN V
Sbjct:   15 PRLNERILSSISRRSVAAHPWHDLEIGPEAPSVFNVV 51
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_MAIZE (Soluble inorganic pyrophosphatase OS=Zea mays GN=IPP PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 8.369e-74
Identity = 104/118 (88.14%), Postives = 112/118 (94.92%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDKK+GLI VDRVLYSSVVYPHNYGF+PRTLCEDNDP+DVL++MQEPV+PG FLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDI EL PHRL EI+RFFEDYK N
Sbjct:   60 GSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFVPRTLCEDNDPMDVLVLMQEPVVPGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDISELSPHRLQEIKRFFEDYKKN 177

HSP 2 Score: 73.1738 bits (178), Expect = 8.369e-74
Identity = 34/51 (66.67%), Postives = 37/51 (72.55%), Query Frame = 3 
Query:  177 ETSSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            E  +     QP   P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN V
Sbjct:    4 EDKTAASAEQPKRAPKLNERILSSLSRRSVAAHPWHDLEIGPDAPAVFNVV 54
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSJ (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. japonica GN=IPP PE=2 SV=1)

HSP 1 Score: 223.402 bits (568), Expect = 3.158e-73
Identity = 103/118 (87.29%), Postives = 111/118 (94.07%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDK +GLI VDRVLYSSVVYPHNYGFIPRTLCED DP+DVL++MQE V+PGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRH+ DIKE+PPHRL EIRRFFEDYK N
Sbjct:   60 GSKVKYELDKATGLIKVDRVLYSSVVYPHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHFRDIKEIPPHRLQEIRRFFEDYKKN 177

HSP 2 Score: 73.1738 bits (178), Expect = 3.158e-73
Identity = 36/53 (67.92%), Postives = 40/53 (75.47%), Query Frame = 3 
Query:  177 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCV 54
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSI (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. indica GN=IPP PE=2 SV=1)

HSP 1 Score: 223.402 bits (568), Expect = 3.158e-73
Identity = 103/118 (87.29%), Postives = 111/118 (94.07%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDK +GLI VDRVLYSSVVYPHNYGFIPRTLCED DP+DVL++MQE V+PGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRH+ DIKE+PPHRL EIRRFFEDYK N
Sbjct:   60 GSKVKYELDKATGLIKVDRVLYSSVVYPHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHFRDIKEIPPHRLQEIRRFFEDYKKN 177

HSP 2 Score: 73.1738 bits (178), Expect = 3.158e-73
Identity = 36/53 (67.92%), Postives = 40/53 (75.47%), Query Frame = 3 
Query:  177 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCV 54
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR2_ARATH (Soluble inorganic pyrophosphatase 2 OS=Arabidopsis thaliana GN=PPA2 PE=2 SV=2)

HSP 1 Score: 222.631 bits (566), Expect = 1.689e-68
Identity = 103/118 (87.29%), Postives = 111/118 (94.07%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            G KVKYELDK SGLI VDRVLYSS+VYPHNYGFIPRT+CED+DP+DVL++MQEPVL G FLRA+AIGLMPMIDQGEKDDKIIAVCADDPE+RHY DIKELPPHRLAEIRRFFEDYK N
Sbjct:   64 GGKVKYELDKNSGLIKVDRVLYSSIVYPHNYGFIPRTICEDSDPMDVLVLMQEPVLTGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEFRHYRDIKELPPHRLAEIRRFFEDYKKN 181

HSP 2 Score: 58.151 bits (139), Expect = 1.689e-68
Identity = 24/35 (68.57%), Postives = 28/35 (80.00%), Query Frame = 3 
Query:  225 LNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            LNER  ++ + RS AAHPWHDLEIGP AP +FNCV
Sbjct:   24 LNERNFAAFTHRSAAAHPWHDLEIGPEAPTVFNCV 58
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_HORVD (Soluble inorganic pyrophosphatase OS=Hordeum vulgare var. distichum GN=IPP PE=2 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 1.831e-64
Identity = 98/118 (83.05%), Postives = 106/118 (89.83%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDKK+GLI VDRVLYSSVVYPHNYGFIPRTLCEDNDP+DVL++MQEPV+PG FLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHY+    L P RL EI+R  EDYK N
Sbjct:   63 GSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFIPRTLCEDNDPMDVLVLMQEPVIPGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYSTSVSLLP-RLQEIKR-LEDYKKN 178

HSP 2 Score: 68.5514 bits (166), Expect = 1.831e-64
Identity = 32/49 (65.31%), Postives = 38/49 (77.55%), Query Frame = 3 
Query:  183 SSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCV 329
            +++ P S+P   P LNERILSS+SRR   AHPWHDLEIGPGAP +FN V
Sbjct:   11 AAQQPTSRPA--PKLNERILSSLSRRGGGAHPWHDLEIGPGAPAVFNVV 57
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR2_CHLRE (Soluble inorganic pyrophosphatase 2 OS=Chlamydomonas reinhardtii GN=ppa2 PE=1 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.948e-55
Identity = 91/118 (77.12%), Postives = 106/118 (89.83%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKXN 696
            GSKVKYELDK +GL  VDR+LYSSVVYPHNYGF+P+TLCED DPLDVL++MQEPV+P CFLRAK IG+M M+DQGE+DDK+IAV ADDPEY+ + DI +LPPHRLAEI+RFFEDYK N
Sbjct:   34 GSKVKYELDKDTGLCFVDRILYSSVVYPHNYGFVPKTLCEDGDPLDVLVLMQEPVVPMCFLRAKPIGVMQMLDQGERDDKLIAVHADDPEYKGFTDISQLPPHRLAEIKRFFEDYKKN 151

HSP 2 Score: 34.6538 bits (78), Expect = 3.948e-55
Identity = 13/24 (54.17%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:  258 RSVAAHPWHDLEIGPGAPKIFNCV 329
            R  A+HPWHDL  G  AP   +CV
Sbjct:    5 RGTASHPWHDLHPGNDAPNFVSCV 28
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIN (Inorganic pyrophosphatase OS=Leptospira interrogans GN=ppa PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.484e-37
Identity = 67/116 (57.76%), Postives = 87/116 (75.00%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYK 690
            GS+ KYE+DK+ G++ +DRVLYSS  YP NYGFIP++ C D DPLD+L++ Q  + P C ++AK IG+M M+D GE+DDKIIAV A+D    H NDI ELPPH   E++ FFEDYK
Sbjct:   27 GSRAKYEVDKEYGILKLDRVLYSSFYYPANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKAKVIGVMRMLDSGEEDDKIIAVAANDMSVNHINDISELPPHFTLELKHFFEDYK 142

HSP 2 Score: 26.9498 bits (58), Expect = 3.484e-37
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = 3 
Query:  273 HPWHDLEIGPGAPKIFNCV 329
            HPWHD+  G   P+I N V
Sbjct:    3 HPWHDISPGDQNPEIVNGV 21
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIC (Inorganic pyrophosphatase OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=ppa PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.484e-37
Identity = 67/116 (57.76%), Postives = 87/116 (75.00%), Query Frame = 1 
Query:  343 GSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYK 690
            GS+ KYE+DK+ G++ +DRVLYSS  YP NYGFIP++ C D DPLD+L++ Q  + P C ++AK IG+M M+D GE+DDKIIAV A+D    H NDI ELPPH   E++ FFEDYK
Sbjct:   27 GSRAKYEVDKEYGILKLDRVLYSSFYYPANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKAKVIGVMRMLDSGEEDDKIIAVAANDMSVNHINDISELPPHFTLELKHFFEDYK 142

HSP 2 Score: 26.9498 bits (58), Expect = 3.484e-37
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = 3 
Query:  273 HPWHDLEIGPGAPKIFNCV 329
            HPWHD+  G   P+I N V
Sbjct:    3 HPWHDISPGDQNPEIVNGV 21
[Close]
BLAST of CK937319 vs. ExPASy Swiss-Prot
Match: IPYR_THEEB (Inorganic pyrophosphatase OS=Thermosynechococcus elongatus (strain BP-1) GN=ppa PE=3 SV=2)

HSP 1 Score: 144.05 bits (362), Expect = 7.042e-34
Identity = 63/117 (53.85%), Postives = 84/117 (71.79%), Query Frame = 1 
Query:  340 GGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYK 690
            GGS+ KYE DK+  +  +DRVLYS+VVYP++YGFIP TL +D DPLD L++M EP  PGC + A+ IG++ MID G++D+KI+ V  DDP Y     +K++ PHRL EI  FF  YK
Sbjct:   25 GGSRNKYEFDKEMKVFALDRVLYSAVVYPYDYGFIPNTLADDGDPLDGLVMMDEPTFPGCVIPARPIGMLEMIDSGDRDEKILCVPVDDPRYAEVKSLKDIAPHRLEEIAEFFRTYK 141
The following BLAST results are available for this feature:
BLAST of CK937319 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 108
Match NameE-valueIdentityDescription
IPYR_SOLTU2.893e-7490.68Soluble inorganic pyrophosphatase OS=Solanum tuber... [more]
IPYR_MAIZE8.369e-7488.14Soluble inorganic pyrophosphatase OS=Zea mays GN=I... [more]
IPYR_ORYSJ3.158e-7387.29Soluble inorganic pyrophosphatase OS=Oryza sativa ... [more]
IPYR_ORYSI3.158e-7387.29Soluble inorganic pyrophosphatase OS=Oryza sativa ... [more]
IPYR2_ARATH1.689e-6887.29Soluble inorganic pyrophosphatase 2 OS=Arabidopsis... [more]
IPYR_HORVD1.831e-6483.05Soluble inorganic pyrophosphatase OS=Hordeum vulga... [more]
IPYR2_CHLRE3.948e-5577.12Soluble inorganic pyrophosphatase 2 OS=Chlamydomon... [more]
IPYR_LEPIN3.484e-3757.76Inorganic pyrophosphatase OS=Leptospira interrogan... [more]
IPYR_LEPIC3.484e-3757.76Inorganic pyrophosphatase OS=Leptospira interrogan... [more]
IPYR_THEEB7.042e-3453.85Inorganic pyrophosphatase OS=Thermosynechococcus e... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004511_D03 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0003_IIF_D03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937319 ID=CK937319; Name=CK937319; organism=Citrus sinensis; type=EST; length=697bp
TCTTGCTTTCCTCTACTTAAGCGCCCGTAGAGGAACTTAACTTTAATATC
TTCGCCATTACGCCTCTCTCTTTTCGAGATCGGGGCGTTTCTTTGTGGTC
TTGAAGCTTCGTTCTCGTTAGGCCCTGTTTGGCTTTCAGTACTTGAACCG
TTACGGTTATGATGGCTCCACCAATTGAGACTTCAAGCAAAACTCCTGTC
TCCCAACCTCCAGCACATCCTCCGCTTAATGAAAGGATACTTTCTTCAAT
GAGCAGGAGGTCTGTTGCTGCACATCCTTGGCATGATCTTGAGATTGGAC
CTGGAGCTCCAAAGATTTTCAATTGCGTGATTGAAATTGGGGGGGAGCAA
GGTGAAATATGAACTTGACAAAAAATCTGGACTGATCATGGTTGATCGTG
TTCTTTACTCTTCAGTTGTGTACCCCCATAACTATGGTTTCATACCTCGT
ACTCTTTGCGAAGACAATGATCCCCTTGATGTCCTGATCATCATGCAGGA
GCCAGTTCTTCCAGGCTGCTTTCTTCGGGCTAAAGCCATCGGACTCATGC
CTATGATTGATCAGGGTGAGAAAGATGATAAGATCATTGCTGTCTGTGCT
GATGATCCCGAGTACCGACACTACAATGACATTAAGGAGCTTCCACCACA
TCGTTTGGCTGAGATCCGCCGCTTCTTTGAAGACTACAAGNAAAATG
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