EY665811

Overview
NameEY665811
Unique NameEY665811
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length932
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 1.720e-58
Identity = 104/138 (75.36%), Postives = 121/138 (87.68%), Query Frame = 3
Query:   45 PYTGTDPFSCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPYICGKYLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMG---RNVCGVDSMDSSVTAGHTT 449
            PYTG+D   CKFDKSKI A++ NFSV+S DE Q++ANL+KHGPLA+GINA +MQTYIGGVSCPYICG++LDHGVL+VGYG+SGFAPIR K+KPYWIIKNSWGENWGENGYYKIC G   RN CGVDSM S+V+A H +
Sbjct:  233 PYTGSDG-KCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICGRHLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVSTVSAVHAS 369          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.228e-57
Identity = 101/137 (73.72%), Postives = 120/137 (87.59%), Query Frame = 3
Query:   45 PYTGTDPFSCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPYICGKYLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSSVTAGHTTSS 455
            PYTG D  +CK DKSKI A++SNFSVIS DE+Q+AANLVK+GPLAV INA +MQTYIGGVSCPYIC + L+HGVL+VGYG++G+AP RFKEKPYWIIKNSWGE WGENG+YKIC GRN+CGVDSM S+V A  +T++
Sbjct:  231 PYTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGVDSMVSTVAATVSTTA 367          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 218.394 bits (555), Expect = 4.685e-56
Identity = 100/136 (73.53%), Postives = 119/136 (87.50%), Query Frame = 3
Query:   45 PYTGTDPFSCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPYICGKYLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSSVTAGHTTS 452
            PYTGTD  SCK D+SKI A++SNFSV+S +EDQ+AANL+K+GPLAV INA +MQTYIGGVSCPYIC + L+HGVL+VGYGS+GF+  R KEKPYWIIKNSWGE+WGENG+YKIC GRN+CGVDS+ S+V A  TTS
Sbjct:  228 PYTGTDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA--TTS 361          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.968e-54
Identity = 98/134 (73.13%), Postives = 113/134 (84.33%), Query Frame = 3
Query:   48 YTGTDPFSCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPYICGKY-LDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSSVTAGHT 446
            YTG D  SCKFDKSK+ A++SNFSV++ DEDQ+AANLVK+GPLAV INA WMQTY+ GVSCPY+C K  LDHGVL+VG+G   +APIR KEKPYWIIKNSWG+NWGE GYYKIC GRNVCGVDSM S+V A  +
Sbjct:  229 YTGRDG-SCKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVAAAQS 361          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: PAPA5_CARPA (Cysteine proteinase (Fragment) OS=Carica papaya PE=3 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 1.563e-43
Identity = 77/95 (81.05%), Postives = 86/95 (90.53%), Query Frame = 3
Query:  168 GPLAVGINAVWMQTYIGGVSCPYICGKYLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSSVTAGHTTS 452
            GPLAV INA +MQTYIGGVSCPYIC + L+HGVL+VGYGS+G+APIR KEKPYW+IKNSWGENWGENGYYKIC GRN+CGVDSM S+V A HTTS
Sbjct:    1 GPLAVAINAAYMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGYAPIRLKEKPYWVIKNSWGENWGENGYYKICRGRNICGVDSMVSTVAAVHTTS 95          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CYSP1_DICDI (Cysteine proteinase 1 OS=Dictyostelium discoideum GN=cprA PE=1 SV=2)

HSP 1 Score: 114.39 bits (285), Expect = 9.528e-25
Identity = 61/140 (43.57%), Postives = 79/140 (56.43%), Query Frame = 3
Query:   15 VGNSGSTTRP--PYTGTDPFSCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPYICGKYLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSS 428
            + N G  T    PYT      C F+ + I A +SNF++I  +E  MA  +V  GPLA+  +AV  Q YIGGV         LDHG+LIVGY +     I  K  PYWI+KNSWG +WGE GY  +  G+N CGV +  S+
Sbjct:  203 IKNGGIQTESSYPYTAETGTQCNFNSANIGAKISNFTMIPKNETVMAGYIVSTGPLAIAADAVEWQFYIGGVFDIPCNPNSLDHGILIVGYSAKN--TIFRKNMPYWIVKNSWGADWGEQGYIYLRRGKNTCGVSNFVST 340          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 108.227 bits (269), Expect = 6.828e-23
Identity = 57/123 (46.34%), Postives = 77/123 (62.60%), Query Frame = 3
Query:   72 CKFDKSKIAAALSNF-SVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPY--ICGKY-LDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSS 428
            C F+++     ++ F  +   +E  M   L+ +GP+++GINA  MQ Y GGVS P+  +C K  LDHGVL+VGYG S + P   K  PYWI+KNSWG  WGE GYY++  G N CGV  M +S
Sbjct:  489 CHFNRTLSHVQVAGFVDLPKGNETAMQEWLLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDY-PNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATS 610          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CATF_HUMAN (Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.893e-21
Identity = 53/123 (43.09%), Postives = 73/123 (59.35%), Query Frame = 3
Query:   69 SCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPY--ICGKYL-DHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSS 428
            SC F   K    +++   +S +E ++AA L K GP++V INA  MQ Y  G+S P   +C  +L DH VL+VGYG+         + P+W IKNSWG +WGE GYY +  G   CGV++M SS
Sbjct:  365 SCNFSAEKAKVYINDSVELSQNEQKLAAWLAKRGPISVAINAFGMQFYRHGISRPLRPLCSPWLIDHAVLLVGYGNRS-------DVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASS 480          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 9.229e-20
Identity = 48/122 (39.34%), Postives = 76/122 (62.30%), Query Frame = 3
Query:   72 CKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPY--ICGKYL-DHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSS 428
            C      +A  +++   ++ DE ++AA L  +  ++VG+NA+ +Q Y  G+S P+   C KYL DH VL+VGYG S       K +P+WI+KNSWG  WGENGY+++  G   CG++++ +S
Sbjct:  200 CHLKTDGVAVYINSSVNLTQDETELAAWLYHNSTISVGMNALLLQFYQHGISHPWWIFCSKYLLDHAVLLVGYGVSE------KNEPFWIVKNSWGVEWGENGYFRMYRGDGSCGINTVATS 315          
BLAST of EY665811 vs. ExPASy Swiss-Prot
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.205e-19
Identity = 49/123 (39.84%), Postives = 73/123 (59.35%), Query Frame = 3
Query:   69 SCKFDKSKIAAALSNFSVISSDEDQMAANLVKHGPLAVGINAVWMQTYIGGVSCPY--ICGK-YLDHGVLIVGYGSSGFAPIRFKEKPYWIIKNSWGENWGENGYYKICMGRNVCGVDSMDSS 428
            +C F        +++   +S +E+++AA L + GP++V INA  MQ Y  G++ P+  +C   ++DH VL+VGYG+           PYW IKNSWG +WGE GYY +  G   CGV++M SS
Sbjct:  343 TCNFSAQMAKVYINDSVELSRNENKIAAWLAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGNRS-------NIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASS 458          
The following BLAST results are available for this feature:
BLAST of EY665811 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 102
Match NameE-valueIdentityDescription
CYSP1_MAIZE1.720e-5875.36Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=... [more]
RD19A_ARATH7.228e-5773.72Cysteine proteinase RD19a OS=Arabidopsis thaliana ... [more]
A494_ARATH4.685e-5673.53Probable cysteine proteinase A494 OS=Arabidopsis t... [more]
CYSP_PEA1.968e-5473.13Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1[more]
PAPA5_CARPA1.563e-4381.05Cysteine proteinase (Fragment) OS=Carica papaya PE... [more]
CYSP1_DICDI9.528e-2543.57Cysteine proteinase 1 OS=Dictyostelium discoideum ... [more]
CPR1_DROME6.828e-2346.34Putative cysteine proteinase CG12163 OS=Drosophila... [more]
CATF_HUMAN4.893e-2143.09Cathepsin F OS=Homo sapiens GN=CTSF PE=1 SV=1[more]
CATL_SCHMA9.229e-2039.34Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=... [more]
CATF_MOUSE1.205e-1939.84Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-095-G12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665811 ID=EY665811; Name=EY665811; organism=Citrus sinensis; type=EST; length=932bp
CACCTGCAGTACCGGTCGGGAATTCCGGGTCGACCACGCGTCCGCCTTAC
ACTGGGACCGACCCTTTTTCCTGCAAATTTGACAAAAGCAAAATTGCTGC
AGCTCTATCTAATTTCAGTGTTATTTCCTCTGATGAAGATCAAATGGCTG
CAAATTTGGTGAAACATGGCCCTCTGGCAGTGGGTATCAATGCCGTTTGG
ATGCAAACATATATTGGAGGAGTTTCATGCCCATACATTTGCGGGAAGTA
TTTGGATCATGGAGTGCTTATCGTGGGCTATGGATCTTCAGGTTTCGCCC
CGATCCGTTTCAAGGAGAAGCCTTACTGGATCATAAAGAACTCCTGGGGA
GAGAACTGGGGAGAGAATGGATATTATAAGATCTGCATGGGTCGCAATGT
CTGTGGGGTCGACTCCATGGACTCATCTGTAACTGCTGGCCATACAACAT
CAAGCTAGACATTATGGAGGCTGTGCTCCCCAAGTGGAGCTTATATACGA
AAAATACAAAAGGATATCCCCCTCACAACCCGGCCCTGAAATGCAGCGCT
CACACTACATCTACCAAGGAGCGCAAGACACGATACACGCCAGAGCCACC
GGTGCCCCGGAGTAACTAAATCAAACCACCCCATACCACCACCGCTAACT
CAATCTCCCTCCCCACTCACACTGACACACCTCCCACCCCAGAACGCCCT
CCACCCACTCCATTCCCCTACCCAACTCAAATGACCCACTGCGAACCACA
AGACCTCCCTCTAACGTGCGGGCCCTGACATGAATGCACCAGACGCCCCA
ATCAATCCAAACCGACCATTAGGCACGAGCGCCCCCGCACCGGTACCAGC
ACCTTAACACCGTGCCCCGAGAAAAAACGCAAAAACATCGCCGCAGTCCC
GACAACCCCCGTCAGCCCCGCTCCCCACACGC
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