EL492566

Overview
NameEL492566
Unique NameEL492566
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length534
Libraries
Library NameType
Citrus sinensis flesh cDNA-AFLP Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10875 contig Csv1_Contig10875:104..633. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE2_CYATE (UDP-glucose 4-epimerase GEPI48 OS=Cyamopsis tetragonoloba PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 2.539e-57
Identity = 95/105 (90.48%), Postives = 100/105 (95.24%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLADGHIAAL KL+DPKIGCEVYNLGTGKGTS LEMV AFE+ASGKKIPLV +GRRPGDAE+VYAST KAERELNWKAKYGIDEMCRDQWNWASKNPYGY
Sbjct:  238 YIHVVDLADGHIAALRKLNDPKIGCEVYNLGTGKGTSVLEMVKAFEQASGKKIPLVMAGRRPGDAEVVYASTNKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 342

HSP 2 Score: 31.187 bits (69), Expect = 2.539e-57
Identity = 14/15 (93.33%), Postives = 14/15 (93.33%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPA 62
            LMPFV QVAVGRRPA
Sbjct:  205 LMPFVQQVAVGRRPA 219

HSP 3 Score: 25.7942 bits (55), Expect = 2.539e-57
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY+T DGTG
Sbjct:  222 VFGNDYTTSDGTG 234
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE3_ARATH (UDP-glucose 4-epimerase 3 OS=Arabidopsis thaliana GN=At4g23920 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.975e-53
Identity = 91/105 (86.67%), Postives = 95/105 (90.48%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLADGHIAAL KLDD KI CEVYNLGTG GTS LEMVAAFEKASGKKIPLV +GRRPGDAE+VYAST KAERELNWKAK GI+EMCRD WNWAS NPYGY
Sbjct:  237 YIHVMDLADGHIAALRKLDDLKISCEVYNLGTGNGTSVLEMVAAFEKASGKKIPLVMAGRRPGDAEVVYASTEKAERELNWKAKNGIEEMCRDLWNWASNNPYGY 341

HSP 2 Score: 29.261 bits (64), Expect = 1.975e-53
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FGTDY TKDGTG
Sbjct:  221 VFGTDYKTKDGTG 233

HSP 3 Score: 28.4906 bits (62), Expect = 1.975e-53
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRP 59
            LMP+V QVAVGRRP
Sbjct:  204 LMPYVQQVAVGRRP 217
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE2_ARATH (UDP-glucose 4-epimerase 2 OS=Arabidopsis thaliana GN=At4g10960 PE=2 SV=3)

HSP 1 Score: 191.815 bits (486), Expect = 3.343e-53
Identity = 90/106 (84.91%), Postives = 96/106 (90.57%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGYE 438
            YIHVIDLADGHIAAL KL+D KIGCEVYNLGTG GTS LEMV AFEKASGKKIPLV +GRRPGDAE+VYAST +AE ELNWKAKYGI+EMCRD WNWAS NPYGY+
Sbjct:  238 YIHVIDLADGHIAALRKLEDCKIGCEVYNLGTGNGTSVLEMVDAFEKASGKKIPLVIAGRRPGDAEVVYASTERAESELNWKAKYGIEEMCRDLWNWASNNPYGYD 343

HSP 2 Score: 29.6462 bits (65), Expect = 3.343e-53
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRP 59
            LMPFV QVAVGRRP
Sbjct:  205 LMPFVQQVAVGRRP 218

HSP 3 Score: 27.7202 bits (60), Expect = 3.343e-53
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY+TKDGTG
Sbjct:  222 VFGNDYNTKDGTG 234
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE1_ARATH (UDP-glucose 4-epimerase 1 OS=Arabidopsis thaliana GN=At1g12780 PE=1 SV=2)

HSP 1 Score: 172.17 bits (435), Expect = 1.761e-44
Identity = 82/107 (76.64%), Postives = 90/107 (84.11%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKL-DDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGYE 438
            YIHV+DLADGHIAAL KL  DPKIGC  YNLGTG+GTS LEMVAAFEKASGKKIP+    RR GDA  VYAST KAE+EL WKAKYG+DEMCRDQW WA+ NP+GY+
Sbjct:  242 YIHVMDLADGHIAALRKLFADPKIGCTAYNLGTGQGTSVLEMVAAFEKASGKKIPIKLCPRRSGDATAVYASTEKAEKELGWKAKYGVDEMCRDQWKWANNNPWGYQ 348

HSP 2 Score: 26.9498 bits (58), Expect = 1.761e-44
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP++ QVAVGR P  + Y
Sbjct:  209 LMPYIQQVAVGRLPELNVY 227
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE1_CYATE (UDP-glucose 4-epimerase GEPI42 OS=Cyamopsis tetragonoloba PE=2 SV=1)

HSP 1 Score: 168.318 bits (425), Expect = 2.235e-43
Identity = 80/107 (74.77%), Postives = 88/107 (82.24%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKL-DDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGYE 438
            YIHV+DLADGHIAAL KL     IGC  YNLGTG+GTS LEMVAAFEKASGKKIP+    RRPGDA  VYAST KAE+EL WKAKYG++EMCRDQW WAS NP+GY+
Sbjct:  245 YIHVMDLADGHIAALRKLFTTDNIGCTAYNLGTGRGTSVLEMVAAFEKASGKKIPIKMCPRRPGDATAVYASTEKAEKELGWKAKYGVEEMCRDQWKWASNNPWGYQ 351

HSP 2 Score: 24.6386 bits (52), Expect = 2.235e-43
Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP++ QVAV R P  + Y
Sbjct:  212 LMPYIQQVAVARLPELNIY 230

HSP 3 Score: 23.0978 bits (48), Expect = 2.235e-43
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = 2 
Query:   71 LFGTDYSTKDGT 106
            ++G DY TKDGT
Sbjct:  229 IYGHDYPTKDGT 240
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE1_PEA (UDP-glucose 4-epimerase OS=Pisum sativum GN=GALE PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 3.510e-42
Identity = 79/106 (74.53%), Postives = 86/106 (81.13%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKL-DDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLADGHIAAL KL     IGC  YNLGTG+G+S LEMVAAFEKASGKKI L    RRPGDA  VYAST KAE+EL WKAKYG++EMCRDQWNWA  NP+GY
Sbjct:  241 YIHVMDLADGHIAALRKLFTSENIGCTAYNLGTGRGSSVLEMVAAFEKASGKKIALKLCPRRPGDATEVYASTAKAEKELGWKAKYGVEEMCRDQWNWAKNNPWGY 346

HSP 2 Score: 26.9498 bits (58), Expect = 3.510e-42
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP++ QVAVGR P  + Y
Sbjct:  208 LMPYIQQVAVGRLPELNVY 226
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE_HUMAN (UDP-glucose 4-epimerase OS=Homo sapiens GN=GALE PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 7.057e-32
Identity = 59/105 (56.19%), Postives = 69/105 (65.71%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLA GHIAAL KL + + GC +YNLGTG G S L+MV A EKASGKKIP     RR GD    YA+   A+ EL W A  G+D MC D W W  +NP G+
Sbjct:  241 YIHVVDLAKGHIAALRKLKE-QCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAQEELGWTAALGLDRMCEDLWRWQKQNPSGF 344

HSP 2 Score: 27.7202 bits (60), Expect = 7.057e-32
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP+V+QVA+GRR A + +
Sbjct:  208 LMPYVSQVAIGRREALNVF 226

HSP 3 Score: 25.7942 bits (55), Expect = 7.057e-32
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY T+DGTG
Sbjct:  225 VFGNDYDTEDGTG 237
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE_PONAB (UDP-glucose 4-epimerase OS=Pongo abelii GN=GALE PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.191e-31
Identity = 59/105 (56.19%), Postives = 68/105 (64.76%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLA GHIAAL KL + + GC +YNLGTG G S L+MV A EKASGKKIP     RR GD    YA+   A  EL W A  G+D MC D W W  +NP G+
Sbjct:  241 YIHVVDLAKGHIAALRKLKE-QCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAHEELGWTAALGLDRMCEDLWRWQKQNPSGF 344

HSP 2 Score: 27.7202 bits (60), Expect = 1.191e-31
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP+V+QVA+GRR A + +
Sbjct:  208 LMPYVSQVAIGRREALNVF 226

HSP 3 Score: 25.7942 bits (55), Expect = 1.191e-31
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY T+DGTG
Sbjct:  225 VFGNDYDTEDGTG 237
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE_RAT (UDP-glucose 4-epimerase OS=Rattus norvegicus GN=Gale PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.389e-31
Identity = 59/104 (56.73%), Postives = 67/104 (64.42%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYG 432
            YIHV+DLA GHIAAL KL + + GC +YNLGTG G S L+MV A EKASGKKIP     RR GD    YA+   A  EL W A  G+D MC D W W  +NP G
Sbjct:  240 YIHVVDLAKGHIAALKKLKE-QCGCRIYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAHEELGWTAALGLDRMCEDLWRWQKQNPSG 342

HSP 2 Score: 27.7202 bits (60), Expect = 3.389e-31
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP+V+QVA+GRR A + +
Sbjct:  207 LMPYVSQVAIGRREALNVF 225

HSP 3 Score: 25.7942 bits (55), Expect = 3.389e-31
Identity = 9/13 (69.23%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY+T+DGTG
Sbjct:  224 VFGDDYATEDGTG 236
[Close]
BLAST of EL492566 vs. ExPASy Swiss-Prot
Match: GALE_MOUSE (UDP-glucose 4-epimerase OS=Mus musculus GN=Gale PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.389e-31
Identity = 59/105 (56.19%), Postives = 67/105 (63.81%), Query Frame = 1 
Query:  121 YIHVIDLADGHIAALHKLDDPKIGCEVYNLGTGKGTSXLEMVAAFEKASGKKIPLVKSGRRPGDAEIVYASTGKAERELNWKAKYGIDEMCRDQWNWASKNPYGY 435
            YIHV+DLA GHIAAL KL + + GC  YNLGTG G S L+MV A EKASGKKIP     RR GD    YA+   A  EL W A  G+D MC D W W  +NP G+
Sbjct:  240 YIHVVDLAKGHIAALKKLKE-QCGCRTYNLGTGTGYSVLQMVQAMEKASGKKIPYKVVARREGDVAACYANPSLAHEELGWTAALGLDRMCEDLWRWQKQNPSGF 343

HSP 2 Score: 27.7202 bits (60), Expect = 3.389e-31
Identity = 11/19 (57.89%), Postives = 16/19 (84.21%), Query Frame = 3 
Query:   18 LMPFVTQVAVGRRPAADSY 74
            LMP+V+QVA+GRR A + +
Sbjct:  207 LMPYVSQVAIGRREALNVF 225

HSP 3 Score: 25.7942 bits (55), Expect = 3.389e-31
Identity = 9/13 (69.23%), Postives = 12/13 (92.31%), Query Frame = 2 
Query:   71 LFGTDYSTKDGTG 109
            +FG DY+T+DGTG
Sbjct:  224 VFGDDYATEDGTG 236
The following BLAST results are available for this feature:
BLAST of EL492566 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
GALE2_CYATE2.539e-5790.48UDP-glucose 4-epimerase GEPI48 OS=Cyamopsis tetrag... [more]
GALE3_ARATH1.975e-5386.67UDP-glucose 4-epimerase 3 OS=Arabidopsis thaliana ... [more]
GALE2_ARATH3.343e-5384.91UDP-glucose 4-epimerase 2 OS=Arabidopsis thaliana ... [more]
GALE1_ARATH1.761e-4476.64UDP-glucose 4-epimerase 1 OS=Arabidopsis thaliana ... [more]
GALE1_CYATE2.235e-4374.77UDP-glucose 4-epimerase GEPI42 OS=Cyamopsis tetrag... [more]
GALE1_PEA3.510e-4274.53UDP-glucose 4-epimerase OS=Pisum sativum GN=GALE P... [more]
GALE_HUMAN7.057e-3256.19UDP-glucose 4-epimerase OS=Homo sapiens GN=GALE PE... [more]
GALE_PONAB1.191e-3156.19UDP-glucose 4-epimerase OS=Pongo abelii GN=GALE PE... [more]
GALE_RAT3.389e-3156.73UDP-glucose 4-epimerase OS=Rattus norvegicus GN=Ga... [more]
GALE_MOUSE3.389e-3156.19UDP-glucose 4-epimerase OS=Mus musculus GN=Gale PE... [more]

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Properties
Property NameValue
Genbank descriptionC3490 Citrus sinensis flesh cDNA-AFLP Library Citrus sinensis cDNA clone ClC3490, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EL492566 ID=EL492566; Name=EL492566; organism=Citrus sinensis; type=EST; length=534bp
CGTGGTTTCNAACAATGCTCATGCCCTTCGTCACTCAAGTTGCTGTTGGC
AGGCGTCCAGCAGCTGACAGCTATTTGGAACTGATTACTCAACAAAGGAT
GGTACTGGGCGTCCGTGATCTACATTCATGTTATTGACTTAGCTGATGGG
CACATTGCTGCATTGCATAAGCTTGATGATCCTAAGATAGGTTGTGAGGT
GTACAACTTGGGAACGGGAAAAGGGACATCANTTTTGGAGATGGTTGCAG
CATTTGAAAAAGCCTCTGGAAAGAAAATTCCTCTTGTAAAGTCTGGGAGG
CGACCCGGTGACGCAGAAATTGTTTATGCATCAACAGGAAAGGCAGAACG
TGAATTGAATTGGAAGGCAAAATATGGCATTGACGAGATGTGTAGGGATC
AATGGAATTGGGCCAGCAAGAACCCTTATGGCTATGAATCTTCCAACTCG
ACTAGCTAATAACTGTCATTTGTCATCCTTNTTAGTAGTTCCTCGCTATA
TACAGNGTCTATTCTGTACACTTACTCAGGACTC
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