EY720682 | Citrus Genome Database

Overview


Name	EY720682
Unique Name	EY720682
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	774

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 5.360e-63
Identity = 75/121 (61.98%), Postives = 96/121 (79.34%), Query Frame = 2

Query:  209 FFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVLISEMKK*ENL 571
            FFQLVNHG+ ++FL+K+K E++ FFNL MEEKKK+WQ P ++EGFGQAFVVSE+QKLDWADLF     PV LRKPHLFPKLP   R+TLE+YS E++S+A+ LI+KM   L  + ++ E L
Sbjct:   83 FFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKL 203

HSP 2 Score: 63.929 bits (154), Expect = 5.360e-63
Identity = 31/62 (50.00%), Postives = 46/62 (74.19%), Query Frame = 3

Query:  561 ERIFEN--GVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            E++F++   V+SMRMN  PPCPQP++V+GL+PHSDS  LT+L+ +N+V G   L+  + G W
Sbjct:  201 EKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEG---LQIKKDGKW 259

HSP 3 Score: 61.6178 bits (148), Expect = 5.360e-63
Identity = 32/68 (47.06%), Postives = 40/68 (58.82%), Query Frame = 1

Query:   16 VQELVKNPALV-VPDRYIRPDQDSPLISDDTLIS-QIPVIDMQXXXXXXXXXXXXAKLDFACKEWGFF 213
            VQE+VK   +  VP RY+R DQD   + DD  +  +IP+IDM+             KLDFACKEWGFF
Sbjct:   17 VQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFF 84

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: NCS1_COPJA (S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 2.975e-32
Identity = 69/144 (47.92%), Postives = 86/144 (59.72%), Query Frame = 2

Query:  209 FFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVLISEMKK*ENL*KWSAKHEDE*LASMSSAREGDG 640
            FFQL+NHG+    +EK+K + E FF L  +EK  Y Q P  MEG+GQAFV SEEQKLDWAD+  ++T PV  R    +P  P S RET+E YSMEL+ +A  L   M + L  E +      +     EDE L SMSS  EG G
Sbjct:   81 FFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCLTGMMAKNLGLESEILTKPLRTVFNREDELLPSMSSCGEGLG 224

HSP 2 Score: 35.8094 bits (81), Expect = 2.975e-32
Identity = 14/23 (60.87%), Postives = 19/23 (82.61%), Query Frame = 3

Query:  636 MGLSPHSDSAALTILVTLNEVGG 704
            +GLSPHSD+  LT+L+ +NEV G
Sbjct:  223 LGLSPHSDATGLTLLIQVNEVNG 245

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: LDOX_VITVI (Leucoanthocyanidin dioxygenase OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.226e-15
Identity = 39/104 (37.50%), Postives = 55/104 (52.88%), Query Frame = 2

Query:  218 LVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLIS 523
            LVNHG+S   + ++K   E FFNL MEEK+KY   Q  G + G+G     +   +L+W D F  L  P   R   ++PK P         YS++L SLA  ++S
Sbjct:   88 LVNHGISDDLINRVKVAGETFFNLPMEEKEKYANDQASGKIAGYGSKLANNASGQLEWEDYFFHLIFPEDKRDMTIWPKTPSDYVPATCEYSVKLRSLATKILS 191

HSP 2 Score: 35.039 bits (79), Expect = 3.226e-15
Identity = 19/49 (38.78%), Postives = 28/49 (57.14%), Query Frame = 3

Query:  594 RMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            ++N  P CPQPE  +G+  H+D +ALT ++  N V G   L+    G W
Sbjct:  219 KINYYPKCPQPELALGVEAHTDVSALTFILH-NMVPG---LQLFYEGKW 263

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: LDOX_ARATH (Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 3.227e-15
Identity = 36/106 (33.96%), Postives = 55/106 (51.89%), Query Frame = 2

Query:  218 LVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKM 529
            L+NHG+    +E++KK  E FF+LS+EEK+KY   Q  G ++G+G     +   +L+W D F  L  P   R   ++PK P    E    Y+  L  LA  +   +
Sbjct:   82 LINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGKIQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLATKVFKAL 187

HSP 2 Score: 36.965 bits (84), Expect = 3.227e-15
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            M++N  P CPQPE  +G+  H+D +ALT ++  N V G   L+    G W
Sbjct:  212 MKINYYPKCPQPELALGVEAHTDVSALTFILH-NMVPG---LQLFYEGKW 257

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: ACCO1_MALDO (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.206e-14
Identity = 39/115 (33.91%), Postives = 59/115 (51.30%), Query Frame = 2

Query:  197 KNGVFFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            +N  FF+LVNHGMST  L+ ++K  +  +  +ME++ K       +   G   V SE   LDW   F +  LP         P L    R+T++ +++ELE LAE L+  + E L
Sbjct:   29 ENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQRFKEM-----VAAKGLDDVQSEIHDLDWESTFFLRHLP--SSNISEIPDLEEEYRKTMKEFAVELEKLAEKLLDLLCENL 136

HSP 2 Score: 38.5058 bits (88), Expect = 3.206e-14
Identity = 16/49 (32.65%), Postives = 31/49 (63.27%), Query Frame = 3

Query:  594 RMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            ++++ PPCP+P+ + GL  HSD+  + +L   ++V G   L+ ++ G W
Sbjct:  158 KVSNYPPCPKPDLIKGLRAHSDAGGIILLFQDDKVSG---LQLLKDGEW 203

HSP 3 Score: 23.0978 bits (48), Expect = 3.206e-14
Identity = 7/12 (58.33%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  178 KLDFACKEWGFF 213
            K++ AC+ WGFF
Sbjct:   23 KINDACENWGFF 34

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: LDOX_MALDO (Leucoanthocyanidin dioxygenase OS=Malus domestica GN=ANS PE=2 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 3.407e-14
Identity = 32/103 (31.07%), Postives = 55/103 (53.40%), Query Frame = 2

Query:  218 LVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLI 520
            LVNHG+S   ++K++K  + FF+L +E+K+KY   Q  G ++G+G     +   +L+W D F     P   R   ++P+ P    E    Y+ +L  LA  ++
Sbjct:   86 LVNHGISDELMDKVRKAGKAFFDLPIEQKEKYANDQASGKIQGYGSKLANNASGQLEWEDYFFHCVYPEDKRDLSIWPQTPADYIEATAEYAKQLRELATKVL 188

HSP 2 Score: 36.965 bits (84), Expect = 3.407e-14
Identity = 20/50 (40.00%), Postives = 29/50 (58.00%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            M++N  P CPQPE  +G+  H+D +ALT ++  N V G   L+    G W
Sbjct:  216 MKINYYPKCPQPELALGVEAHTDVSALTFILH-NMVPG---LQLFYEGKW 261

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FLS_MATIN (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana PE=2 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 9.768e-14
Identity = 33/110 (30.00%), Postives = 58/110 (52.73%), Query Frame = 2

Query:  212 FQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            FQ+VNHG+ T  +++L+K    FF L   EK+   +  G +EG+G+   +  +++    D   + T P        +PK PP  RE  E Y+  +++L+E ++  + E L
Sbjct:   29 FQVVNHGIPTELIQRLQKVGREFFELPEAEKRSCAREAGSVEGYGRRIELDIKKRKGIVDQIYLSTWPPSSVNYRYWPKSPPDYREVNEEYARHVKTLSEKIMEWLSEGL 138

HSP 2 Score: 35.039 bits (79), Expect = 9.768e-14
Identity = 16/38 (42.11%), Postives = 23/38 (60.53%), Query Frame = 3

Query:  591 MRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGG 704
            M +N  PP P  +   GL PH+D   LT+++T NE+ G
Sbjct:  155 MNINHYPPYPHSDSFNGLEPHTDINGLTLIIT-NEIPG 191

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.126e-13
Identity = 34/116 (29.31%), Postives = 56/116 (48.28%), Query Frame = 2

Query:  209 FFQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVH--LRKPHLFPKLPPSLRETLEVYS---MELESLAENLISKMGEVL 541
            FFQ+VNHG+    L    + V+ FF + + EK++  +HPG+  G+  +F      KL W +  S  + P    L   ++   L    R   EVY+    E+  L+  ++  +GE L
Sbjct:   71 FFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFASKLPWKETLSFRSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESL 186

HSP 2 Score: 41.5874 bits (96), Expect = 2.126e-13
Identity = 25/59 (42.37%), Postives = 31/59 (52.54%), Query Frame = 3

Query:  564 RIFENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            R FE     MR+N  PPC +P + +G  PH D  +LTIL   N VGG   L+    G W
Sbjct:  195 RFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDN-VGG---LQVHTEGRW 249

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: FL3H_MALDO (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.489e-13
Identity = 31/110 (28.18%), Postives = 52/110 (47.27%), Query Frame = 2

Query:  212 FQLVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYWQHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLISKMGEVL 541
            FQ+V+HG+    + ++      FF L  EEK ++    G   GF  +  +  E   DW ++ +  + P+  R    +P  P + RE  + YS EL  LA  L+  + E +
Sbjct:   71 FQIVDHGVDAELISEMTGLAREFFALPSEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRHRDYSRWPDKPEAWREVTKKYSDELMGLACKLLGVLSEAM 180

HSP 2 Score: 34.6538 bits (78), Expect = 2.489e-13
Identity = 14/32 (43.75%), Postives = 22/32 (68.75%), Query Frame = 3

Query:  609 PPCPQPEKVMGLSPHSDSAALTILVTLNEVGG 704
            P CPQP+  +GL  H+D   +T+L+  ++VGG
Sbjct:  204 PKCPQPDLTLGLKRHTDPGTITLLLQ-DQVGG 234

HSP 3 Score: 26.9498 bits (58), Expect = 2.489e-13
Identity = 11/53 (20.75%), Postives = 26/53 (49.06%), Query Frame = 1

Query:   58 RYIRPDQDSPLISDDTLISQIPVIDMQXXXXXXXXXXXXAK-LDFACKEWGFF 213
            +++R + + P ++ +   ++IP+I +              K +  AC++WG F
Sbjct:   19 KFVRDEDERPKVAYNDFSNEIPIISLAGIDEVEGRRGEICKKIVAACEDWGIF 71

[Close]

BLAST of EY720682 vs. ExPASy Swiss-Prot
Match: LDOX_PETHY (Leucoanthocyanidin dioxygenase OS=Petunia hybrida GN=ANT17 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.748e-13
Identity = 32/104 (30.77%), Postives = 54/104 (51.92%), Query Frame = 2

Query:  218 LVNHGMSTAFLEKLKKEVEGFFNLSMEEKKKYW--QHPGDMEGFGQAFVVSEEQKLDWADLFSMLTLPVHLRKPHLFPKLPPSLRETLEVYSMELESLAENLIS 523
            LVNHG+S   + ++K   E FF+  +EEK+KY   Q  G+++G+G     S   +L+W D F     P   R   ++PK P         Y+ ++ +LA  +++
Sbjct:   86 LVNHGISDELINRVKVAGETFFDQPVEEKEKYANDQANGNVQGYGSKLANSACGQLEWEDYFFHCAFPEDKRDLSIWPKNPTDYTPATSEYAKQIRALATKILT 189

HSP 2 Score: 37.3502 bits (85), Expect = 2.748e-13
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3

Query:  573 ENGVRSMRMND*PPCPQPEKVMGLSPHSDSAALTILVTLNEVGGSSRLKRMERGSW 740
            E+ +  M++N  P CPQPE  +G+  H+D +ALT ++  N V G   L+    G W
Sbjct:  210 EDLLLQMKINYYPKCPQPELALGVEAHTDVSALTFILH-NMVPG---LQLFYEGQW 261

The following BLAST results are available for this feature:

BLAST of EY720682 vs. ExPASy Swiss-Prot

BLAST of EY720682 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29


Match Name	E-value	Identity	Description
SRG1_ARATH	5.360e-63	61.98	Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 ...	[more]
NCS1_COPJA	2.975e-32	47.92	S-norcoclaurine synthase 1 OS=Coptis japonica GN=N...	[more]
LDOX_VITVI	3.226e-15	37.50	Leucoanthocyanidin dioxygenase OS=Vitis vinifera P...	[more]
LDOX_ARATH	3.227e-15	33.96	Leucoanthocyanidin dioxygenase OS=Arabidopsis thal...	[more]
ACCO1_MALDO	3.206e-14	33.91	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Mal...	[more]
LDOX_MALDO	3.407e-14	31.07	Leucoanthocyanidin dioxygenase OS=Malus domestica ...	[more]
FLS_MATIN	9.768e-14	30.00	Flavonol synthase/flavanone 3-hydroxylase (Fragmen...	[more]
GAO1D_WHEAT	2.126e-13	29.31	Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN...	[more]
FL3H_MALDO	2.489e-13	28.18	Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus d...	[more]
LDOX_PETHY	2.748e-13	30.77	Leucoanthocyanidin dioxygenase OS=Petunia hybrida ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-012-H09-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720682 ID=EY720682; Name=EY720682; organism=Citrus sinensis; type=EST; length=774bp
GCGACCACGCGTCCGGTTCAAGAGTTGGTCAAGAATCCTGCGTTAGTTGT
CCCCGACAGATACATACGTCCAGATCAAGATTCACCACTCATTTCTGATG
ATACTTTGATTTCACAGATACCAGTTATTGACATGCAAAGCTTACTTTCT
GAAGAATCAATGGATTCTGAGCTAGCCAAGCTCGACTTCGCATGCAAAGA
ATGGGGTTTTTTTCCAGCTGGTAAATCATGGAATGAGCACAGCATTTCTA
GAGAAATTAAAGAAAGAGGTTGAGGGATTCTTCAATCTCAGCATGGAAGA
AAAGAAAAAATATTGGCAGCATCCAGGAGATATGGAAGGCTTTGGACAAG
CCTTTGTTGTATCTGAGGAACAAAAGCTTGATTGGGCTGACCTTTTCTCC
ATGCTGACTCTTCCAGTTCATCTGAGGAAGCCACACTTATTTCCTAAGCT
CCCTCCTTCCCTCAGAGAGACCTTGGAAGTGTACTCAATGGAATTAGAAT
CCCTAGCCGAGAACCTAATATCGAAAATGGGAGAAGTGTTAATATCAGAG
ATGAAGAAATGAGAGAATCTTTGAAAATGGAGTGCGAAGCATGAGGATGA
ATGATTAGCCTCCATGTCCTCAGCCCGAGAAGGTGATGGGCCTGTCTCCT
CATTCTGATTCAGCTGCACTTACCATTCTGGTGACACTTAATGAAGTTGG
AGGGTCCTCCCGGTTAAAAAGGATGGAAAGGGGATCCTGGTGAGCGGGTC
GCGGATGGTTTCATTGGCTCCATT