EG358386

Overview
NameEG358386
Unique NameEG358386
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length557
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12188 contig Csv1_Contig12188:1208..1764. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC2_PEA (Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pisum sativum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 346.28 bits (887), Expect = 5.962e-95
Identity = 167/185 (90.27%), Postives = 175/185 (94.59%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLKKEKA+I+ PDRVVIANGPAFGCVLM DFLKAL+KRLK N  AYENYHRI+VP+G P K   KEPLRVNV+FQHIQKMLSSETAVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGATLGYAQ+VPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP
Sbjct:  138 SLLLKKEKAIIVMPDRVVIANGPAFGCVLMNDFLKALAKRLKHNNVAYENYHRIFVPDGTPLKSASKEPLRVNVMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 322
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC1_TOBAC (Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nicotiana tabacum GN=PDC1 PE=2 SV=1)

HSP 1 Score: 325.479 bits (833), Expect = 1.089e-88
Identity = 154/176 (87.50%), Postives = 164/176 (93.18%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYT 530
            SLLLKKEKA+I+QPDRV I NGPAFGCVLM+DFL AL+KRLK N TA+ENYHRIYVPEG P KCEPKE LRVNVLFQHIQ MLS ++ VIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRVIACIGDGSFQVTAQD+STMLRCGQ+TIIFLINNGGYT
Sbjct:  243 SLLLKKEKAIIVQPDRVTIGNGPAFGCVLMRDFLAALAKRLKHNPTAFENYHRIYVPEGHPLKCEPKEALRVNVLFQHIQNMLSGDSVVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAAPEKRVIACIGDGSFQVTAQDISTMLRCGQRTIIFLINNGGYT 418
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC2_TOBAC (Pyruvate decarboxylase isozyme 2 OS=Nicotiana tabacum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 1.204e-87
Identity = 160/211 (75.83%), Postives = 172/211 (81.52%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCG--------------------------YEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLL+KKEK++I+QPDRVVI NGPAFGCVLMKDFL  L+K++K N TAYENY RI+VPEG P K EP EPLRVNVLFQHIQKMLS ETAVIAETGDSWFNCQKLKLP+GCG                          YEFQMQYGSIGWSVGATLGYAQSVP+KRVI+CIGDGSFQVTAQDVSTM+RC QK IIFLINNGGYTIEVEIHDGP
Sbjct:  321 SLLIKKEKSIIVQPDRVVIGNGPAFGCVLMKDFLSELAKKIKKNETAYENYRRIFVPEGTPLKSEPNEPLRVNVLFQHIQKMLSDETAVIAETGDSWFNCQKLKLPEGCGYVTNNSLSAWYPFYLQTLEEKSSCCRYEFQMQYGSIGWSVGATLGYAQSVPKKRVISCIGDGSFQVTAQDVSTMIRCEQKNIIFLINNGGYTIEVEIHDGP 531
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSJ (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. japonica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 2.054e-87
Identity = 148/185 (80.00%), Postives = 172/185 (92.97%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDRVV+ NGPAFGC+LM +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVI+CIGDGSFQ+TAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  338 SLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 522
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSI (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. indica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 2.054e-87
Identity = 148/185 (80.00%), Postives = 172/185 (92.97%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDRVV+ NGPAFGC+LM +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVI+CIGDGSFQ+TAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  338 SLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 522
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSJ (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. japonica GN=PDC2 PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 169/185 (91.35%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKK+KA+I+QP+RV++ NGPAFGCV+MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGA LGYAQ   +KRVIACIGDGSFQVTAQDVSTM+RC Q +IIFLINNGGYTIEVEIHDGP
Sbjct:  339 SFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGP 523
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSI (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. indica GN=PDC2 PE=2 SV=2)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 169/185 (91.35%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKK+KA+I+QP+RV++ NGPAFGCV+MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGA LGYAQ   +KRVIACIGDGSFQVTAQDVSTM+RC Q +IIFLINNGGYTIEVEIHDGP
Sbjct:  339 SFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGP 523
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC1_MAIZE (Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PDC1 PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 173/185 (93.51%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDR+V+ +GPAFGC+LM +FL+AL+KRL+ NTTAY+NY RI+VP+ +PP  +P EPLRVNVLF+HI+ MLS ++AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVIACIGDGSFQVTAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  343 SLLLKREKAVIVQPDRMVVGDGPAFGCILMPEFLRALAKRLRRNTTAYDNYRRIFVPDREPPNGKPNEPLRVNVLFKHIKGMLSGDSAVVAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 527
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC3_ORYSJ (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. japonica GN=PDC3 PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.166e-82
Identity = 145/187 (77.54%), Postives = 168/187 (89.84%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSET--AVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKKEKAV++QPDRV + NGPAFGCV+M+DFL  L+KR++ NTTA++NY RI+VPEGQ P+CE  E LRVNVLF+HIQ+M+      AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGA LGYAQ+V +KRV+ACIGDGSFQVTAQDVSTMLRCGQ++IIFLINNGGYTIEVEIHDGP
Sbjct:  319 SCLLKKEKAVVVQPDRVTVGNGPAFGCVMMRDFLSELAKRVRKNTTAFDNYKRIFVPEGQLPECEAGEALRVNVLFKHIQRMIGGTEIGAVMAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGALLGYAQAV-QKRVVACIGDGSFQVTAQDVSTMLRCGQRSIIFLINNGGYTIEVEIHDGP 504
[Close]
BLAST of EG358386 vs. ExPASy Swiss-Prot
Match: PDC3_ORYSI (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. indica GN=PDC3 PE=2 SV=2)

HSP 1 Score: 305.449 bits (781), Expect = 1.166e-82
Identity = 145/187 (77.54%), Postives = 168/187 (89.84%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSET--AVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKKEKAV++QPDRV + NGPAFGCV+M+DFL  L+KR++ NTTA++NY RI+VPEGQ P+CE  E LRVNVLF+HIQ+M+      AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGA LGYAQ+V +KRV+ACIGDGSFQVTAQDVSTMLRCGQ++IIFLINNGGYTIEVEIHDGP
Sbjct:  319 SCLLKKEKAVVVQPDRVTVGNGPAFGCVMMRDFLSELAKRVRKNTTAFDNYKRIFVPEGQLPECEAGEALRVNVLFKHIQRMIGGAEIGAVMAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGALLGYAQAV-QKRVVACIGDGSFQVTAQDVSTMLRCGQRSIIFLINNGGYTIEVEIHDGP 504
The following BLAST results are available for this feature:
BLAST of EG358386 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41
Match NameE-valueIdentityDescription
PDC2_PEA5.962e-9590.27Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pis... [more]
PDC1_TOBAC1.089e-8887.50Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nic... [more]
PDC2_TOBAC1.204e-8775.83Pyruvate decarboxylase isozyme 2 OS=Nicotiana taba... [more]
PDC1_ORYSJ2.054e-8780.00Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC1_ORYSI2.054e-8780.00Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC2_ORYSJ3.503e-8780.00Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC2_ORYSI3.503e-8780.00Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC1_MAIZE3.503e-8780.00Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PD... [more]
PDC3_ORYSJ1.166e-8277.54Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s... [more]
PDC3_ORYSI1.166e-8277.54Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s... [more]

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Properties
Property NameValue
Genbank description6e8 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Blonde cadenera cultivar Citrus sinensis cDNA clone Cl0006e8, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358386 ID=EG358386; Name=EG358386; organism=Citrus sinensis; type=EST; length=557bp
ACTCTCTTCTTCTCAAGAAGGAGAAGGCAGTCATTCTGCAGCCTGATCGT
GTGGTGATTGCTAATGGACCGGCATTTGGTTGTGTTTTGATGAAGGATTT
CCTCAAAGCACTTTCAAAGCGGCTCAAAAGCAACACTACTGCTTATGAGA
ATTACCACAGGATTTATGTTCCTGAGGGGCAACCTCCGAAGTGTGAACCC
AAAGAGCCCTTGAGAGTGAATGTTCTGTTCCAACATATACAGAAGATGCT
GTCTAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCTTGGTTTAACT
GCCAGAAACTGAAATTGCCTAAGGGATGCGGGTATGAGTTCCAAATGCAA
TATGGATCAATTGGTTGGTCTGTTGGTGCTACTCTTGGATATGCACAGTC
TGTGCCAGAGAAGCGTGTCATTGCTTGCATAGGTGATGGTAGCTTCCAGG
TAACTGCACAAGATGTGTCAACAATGCTGCGATGTGGGCAGAAGACTATA
ATCTTCCTGATTAACAATGGTGGTTACACCATTGAAGTTGAGATCCATGA
TGGACCG
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