EG358318

Overview
NameEG358318
Unique NameEG358318
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length557
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12188 contig Csv1_Contig12188:1208..1764. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC2_PEA (Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pisum sativum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 346.28 bits (887), Expect = 5.962e-95
Identity = 167/185 (90.27%), Postives = 175/185 (94.59%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLKKEKA+I+ PDRVVIANGPAFGCVLM DFLKAL+KRLK N  AYENYHRI+VP+G P K   KEPLRVNV+FQHIQKMLSSETAVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGATLGYAQ+VPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP
Sbjct:  138 SLLLKKEKAIIVMPDRVVIANGPAFGCVLMNDFLKALAKRLKHNNVAYENYHRIFVPDGTPLKSASKEPLRVNVMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 322
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC1_TOBAC (Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nicotiana tabacum GN=PDC1 PE=2 SV=1)

HSP 1 Score: 325.479 bits (833), Expect = 1.089e-88
Identity = 154/176 (87.50%), Postives = 164/176 (93.18%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYT 530
            SLLLKKEKA+I+QPDRV I NGPAFGCVLM+DFL AL+KRLK N TA+ENYHRIYVPEG P KCEPKE LRVNVLFQHIQ MLS ++ VIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQ+ PEKRVIACIGDGSFQVTAQD+STMLRCGQ+TIIFLINNGGYT
Sbjct:  243 SLLLKKEKAIIVQPDRVTIGNGPAFGCVLMRDFLAALAKRLKHNPTAFENYHRIYVPEGHPLKCEPKEALRVNVLFQHIQNMLSGDSVVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAAPEKRVIACIGDGSFQVTAQDISTMLRCGQRTIIFLINNGGYT 418
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC2_TOBAC (Pyruvate decarboxylase isozyme 2 OS=Nicotiana tabacum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 1.204e-87
Identity = 160/211 (75.83%), Postives = 172/211 (81.52%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCG--------------------------YEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLL+KKEK++I+QPDRVVI NGPAFGCVLMKDFL  L+K++K N TAYENY RI+VPEG P K EP EPLRVNVLFQHIQKMLS ETAVIAETGDSWFNCQKLKLP+GCG                          YEFQMQYGSIGWSVGATLGYAQSVP+KRVI+CIGDGSFQVTAQDVSTM+RC QK IIFLINNGGYTIEVEIHDGP
Sbjct:  321 SLLIKKEKSIIVQPDRVVIGNGPAFGCVLMKDFLSELAKKIKKNETAYENYRRIFVPEGTPLKSEPNEPLRVNVLFQHIQKMLSDETAVIAETGDSWFNCQKLKLPEGCGYVTNNSLSAWYPFYLQTLEEKSSCCRYEFQMQYGSIGWSVGATLGYAQSVPKKRVISCIGDGSFQVTAQDVSTMIRCEQKNIIFLINNGGYTIEVEIHDGP 531
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSJ (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. japonica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 2.054e-87
Identity = 148/185 (80.00%), Postives = 172/185 (92.97%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDRVV+ NGPAFGC+LM +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVI+CIGDGSFQ+TAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  338 SLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 522
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSI (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. indica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 2.054e-87
Identity = 148/185 (80.00%), Postives = 172/185 (92.97%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDRVV+ NGPAFGC+LM +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVI+CIGDGSFQ+TAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  338 SLLLKREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 522
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSJ (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. japonica GN=PDC2 PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 169/185 (91.35%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKK+KA+I+QP+RV++ NGPAFGCV+MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGA LGYAQ   +KRVIACIGDGSFQVTAQDVSTM+RC Q +IIFLINNGGYTIEVEIHDGP
Sbjct:  339 SFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGP 523
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSI (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. indica GN=PDC2 PE=2 SV=2)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 169/185 (91.35%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKK+KA+I+QP+RV++ NGPAFGCV+MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGA LGYAQ   +KRVIACIGDGSFQVTAQDVSTM+RC Q +IIFLINNGGYTIEVEIHDGP
Sbjct:  339 SFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDGP 523
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC1_MAIZE (Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PDC1 PE=2 SV=1)

HSP 1 Score: 320.472 bits (820), Expect = 3.503e-87
Identity = 148/185 (80.00%), Postives = 173/185 (93.51%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            SLLLK+EKAVI+QPDR+V+ +GPAFGC+LM +FL+AL+KRL+ NTTAY+NY RI+VP+ +PP  +P EPLRVNVLF+HI+ MLS ++AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGATLGYAQ+  +KRVIACIGDGSFQVTAQDVSTMLRCGQK+IIFLINNGGYTIEVEIHDGP
Sbjct:  343 SLLLKREKAVIVQPDRMVVGDGPAFGCILMPEFLRALAKRLRRNTTAYDNYRRIFVPDREPPNGKPNEPLRVNVLFKHIKGMLSGDSAVVAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVIACIGDGSFQVTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGP 527
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC3_ORYSJ (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. japonica GN=PDC3 PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.166e-82
Identity = 145/187 (77.54%), Postives = 168/187 (89.84%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSET--AVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKKEKAV++QPDRV + NGPAFGCV+M+DFL  L+KR++ NTTA++NY RI+VPEGQ P+CE  E LRVNVLF+HIQ+M+      AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGA LGYAQ+V +KRV+ACIGDGSFQVTAQDVSTMLRCGQ++IIFLINNGGYTIEVEIHDGP
Sbjct:  319 SCLLKKEKAVVVQPDRVTVGNGPAFGCVMMRDFLSELAKRVRKNTTAFDNYKRIFVPEGQLPECEAGEALRVNVLFKHIQRMIGGTEIGAVMAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGALLGYAQAV-QKRVVACIGDGSFQVTAQDVSTMLRCGQRSIIFLINNGGYTIEVEIHDGP 504
[Close]
BLAST of EG358318 vs. ExPASy Swiss-Prot
Match: PDC3_ORYSI (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. indica GN=PDC3 PE=2 SV=2)

HSP 1 Score: 305.449 bits (781), Expect = 1.166e-82
Identity = 145/187 (77.54%), Postives = 168/187 (89.84%), Query Frame = 3 
Query:    3 SLLLKKEKAVILQPDRVVIANGPAFGCVLMKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSET--AVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQSVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGP 557
            S LLKKEKAV++QPDRV + NGPAFGCV+M+DFL  L+KR++ NTTA++NY RI+VPEGQ P+CE  E LRVNVLF+HIQ+M+      AV+AETGDSWFNCQKL+LP+GCGYEFQMQYGSIGWSVGA LGYAQ+V +KRV+ACIGDGSFQVTAQDVSTMLRCGQ++IIFLINNGGYTIEVEIHDGP
Sbjct:  319 SCLLKKEKAVVVQPDRVTVGNGPAFGCVMMRDFLSELAKRVRKNTTAFDNYKRIFVPEGQLPECEAGEALRVNVLFKHIQRMIGGAEIGAVMAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGALLGYAQAV-QKRVVACIGDGSFQVTAQDVSTMLRCGQRSIIFLINNGGYTIEVEIHDGP 504
The following BLAST results are available for this feature:
BLAST of EG358318 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41
Match NameE-valueIdentityDescription
PDC2_PEA5.962e-9590.27Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pis... [more]
PDC1_TOBAC1.089e-8887.50Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nic... [more]
PDC2_TOBAC1.204e-8775.83Pyruvate decarboxylase isozyme 2 OS=Nicotiana taba... [more]
PDC1_ORYSJ2.054e-8780.00Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC1_ORYSI2.054e-8780.00Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC2_ORYSJ3.503e-8780.00Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC2_ORYSI3.503e-8780.00Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC1_MAIZE3.503e-8780.00Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PD... [more]
PDC3_ORYSJ1.166e-8277.54Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s... [more]
PDC3_ORYSI1.166e-8277.54Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s... [more]

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Properties
Property NameValue
Genbank description6d8 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl0006d8, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358318 ID=EG358318; Name=EG358318; organism=Citrus sinensis; type=EST; length=557bp
ACTCTCTTCTTCTCAAGAAGGAGAAGGCAGTCATTCTGCAGCCTGATCGT
GTGGTGATTGCTAATGGACCGGCATTTGGTTGTGTTTTGATGAAGGATTT
CCTCAAAGCACTTTCAAAGCGGCTCAAAAGCAACACTACTGCTTATGAGA
ATTACCACAGGATTTATGTTCCTGAGGGGCAACCTCCGAAGTGTGAACCC
AAAGAGCCCTTGAGAGTGAATGTTCTGTTCCAACATATACAGAAGATGCT
GTCTAGTGAAACTGCTGTGATTGCTGAGACAGGGGACTCTTGGTTTAACT
GCCAGAAACTGAAATTGCCTAAGGGATGCGGGTATGAGTTCCAAATGCAA
TATGGATCAATTGGTTGGTCTGTTGGTGCTACTCTTGGATATGCACAGTC
TGTGCCAGAGAAGCGTGTCATTGCTTGCATAGGTGATGGTAGCTTCCAGG
TAACTGCACAAGATGTGTCAACAATGCTGCGATGTGGGCAGAAGACTATA
ATCTTCCTGATTAACAATGGTGGTTACACCATTGAAGTTGAGATCCATGA
TGGACCG
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