CX672009

Overview
NameCX672009
Unique NameCX672009
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length833
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORT9 (tRNA-specific 2-thiouridylase mnmA OS=Borrelia turicatae (strain 91E135) GN=mnmA PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.844e-24
Identity = 43/91 (47.25%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:  271 ECPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVVHSSADH 540
            ECP +ED+ Y +AV  + +VP E+  L DEY+K VV+Y IEE     TP+PD+ CN RIKFG F D I+   +D + +GHYA + + + DH
Sbjct:   46 ECPWKEDINYVEAVCKKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKINE-HYDLIVTGHYAKIENKN-DH 134

HSP 2 Score: 45.4394 bits (106), Expect = 2.844e-24
Identity = 23/51 (45.10%), Postives = 34/51 (66.67%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCI-AFYLKIWFQTSRTFGLNAHWKK 289
            +++AVLLSGGVDSSVAL ++   G+  I  +YLKIW +   ++     WK+
Sbjct:    1 MQIAVLLSGGVDSSVALYIMIQKGYKNIKCYYLKIWLEDELSYIGECPWKE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORHD (tRNA-specific 2-thiouridylase mnmA OS=Borrelia hermsii (strain DAH) GN=mnmA PE=3 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 3.701e-24
Identity = 42/84 (50.00%), Postives = 56/84 (66.67%), Query Frame = 1 
Query:  271 ECPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            ECP EED+ Y +AV  + +VP E+  L DEY+K VV+Y IEE     TP+PD+ CN RIKFG F D I+   +D + +GHYA +
Sbjct:   46 ECPWEEDINYVEAVCTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKINE-HYDLIVTGHYAKI 128

HSP 2 Score: 44.2838 bits (103), Expect = 3.701e-24
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCI-AFYLKIWFQTSRTFGLNAHWKK 289
            +K+AVLLSGGVDSSVAL  +   G+  I  +YLKIW +   ++     W++
Sbjct:    1 MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDELSYIGECPWEE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORBZ (tRNA-specific 2-thiouridylase mnmA OS=Borrelia burgdorferi (strain ZS7) GN=mnmA PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.338e-23
Identity = 38/83 (45.78%), Postives = 56/83 (67.47%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            CP +ED+ Y +A+ ++ +VP E+ +   EY+  VVSY IEE     TP+PD+ CN RIKFG F + I+S ++D V +GHYA +
Sbjct:   47 CPWQEDLNYVEAICNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKINS-QYDLVVTGHYAKI 128

HSP 2 Score: 47.7506 bits (112), Expect = 2.338e-23
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVAL-RLLNAAGHSCIAFYLKIWFQTSRTFGLNAHWKK 289
            +K+AVLLSGGVDSSVAL R++N    +   +YLKIW +   ++  N  W++
Sbjct:    1 MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDELSYIGNCPWQE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORBU (tRNA-specific 2-thiouridylase mnmA OS=Borrelia burgdorferi GN=mnmA PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.338e-23
Identity = 38/83 (45.78%), Postives = 56/83 (67.47%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            CP +ED+ Y +A+ ++ +VP E+ +   EY+  VVSY IEE     TP+PD+ CN RIKFG F + I+S ++D V +GHYA +
Sbjct:   47 CPWQEDLNYVEAICNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKINS-QYDLVVTGHYAKI 128

HSP 2 Score: 47.7506 bits (112), Expect = 2.338e-23
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVAL-RLLNAAGHSCIAFYLKIWFQTSRTFGLNAHWKK 289
            +K+AVLLSGGVDSSVAL R++N    +   +YLKIW +   ++  N  W++
Sbjct:    1 MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDELSYIGNCPWQE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORAP (tRNA-specific 2-thiouridylase mnmA OS=Borrelia afzelii (strain PKo) GN=mnmA PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.252e-22
Identity = 37/83 (44.58%), Postives = 54/83 (65.06%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            CP +ED+ Y +AV  + +VP E+ +   EY+  VVSY I+E     TP+PD+ CN RIKFG F + I+  ++D V +GHYA +
Sbjct:   47 CPWQEDLNYVEAVCSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKINE-QYDLVVTGHYAKI 128

HSP 2 Score: 46.9802 bits (110), Expect = 3.252e-22
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVAL-RLLNAAGHSCIAFYLKIWFQTSRTFGLNAHWKK 289
            +K+AVLLSGGVDSSVAL R++N    S   +YLKIW +   ++  +  W++
Sbjct:    1 MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDELSYIGSCPWQE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORDL (tRNA-specific 2-thiouridylase mnmA OS=Borrelia duttonii (strain Ly) GN=mnmA PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.252e-22
Identity = 37/84 (44.05%), Postives = 56/84 (66.67%), Query Frame = 1 
Query:  271 ECPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            ECP +ED+ Y  +V  + +VP E+ +L +EY+  VV+Y IEE     TP+PD+ CN RIKFG F + I+  ++D + +GHYA +
Sbjct:   46 ECPWKEDITYVDSVCKKFNVPYEIINLQEEYYNRVVTYAIEELKMGNTPSPDIFCNQRIKFGAFFEKINE-KYDLIVTGHYAKI 128

HSP 2 Score: 45.4394 bits (106), Expect = 3.252e-22
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCI-AFYLKIWFQTSRTFGLNAHWKK 289
            +K+A+LLSGGVDSSVAL  +   G+  I  +YLKIW +   ++     WK+
Sbjct:    1 MKIAILLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDELSYIGECPWKE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORRA (tRNA-specific 2-thiouridylase mnmA OS=Borrelia recurrentis (strain A1) GN=mnmA PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 9.317e-22
Identity = 36/84 (42.86%), Postives = 56/84 (66.67%), Query Frame = 1 
Query:  271 ECPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            ECP +ED+ Y  +V  + +VP ++ +L +EY+  VV+Y IEE     TP+PD+ CN RIKFG F + I+  ++D + +GHYA +
Sbjct:   46 ECPWKEDITYVDSVCKKFNVPYKIINLQEEYYNRVVTYAIEELKMGNTPSPDIFCNQRIKFGAFFEKINE-KYDLIVTGHYAKI 128

HSP 2 Score: 45.4394 bits (106), Expect = 9.317e-22
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCI-AFYLKIWFQTSRTFGLNAHWKK 289
            +K+A+LLSGGVDSSVAL  +   G+  I  +YLKIW +   ++     WK+
Sbjct:    1 MKIAILLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDELSYIGECPWKE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BORGA (tRNA-specific 2-thiouridylase mnmA OS=Borrelia garinii GN=mnmA PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.212e-21
Identity = 36/83 (43.37%), Postives = 54/83 (65.06%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPLEV-HLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            CP +ED+ Y +AV ++ ++P E+ +   EY+  VV Y IEE     TP+PD+ CN RIKFG F + I+  ++D V +GHYA +
Sbjct:   47 CPWQEDLNYVEAVCNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKINE-QYDLVVTGHYARI 128

HSP 2 Score: 45.8246 bits (107), Expect = 1.212e-21
Identity = 22/51 (43.14%), Postives = 35/51 (68.63%), Query Frame = 2 
Query:  140 LKVAVLLSGGVDSSVAL-RLLNAAGHSCIAFYLKIWFQTSRTFGLNAHWKK 289
            +K+AVLLSGGVDSS+AL +++N    +   +YLKIW +   ++  N  W++
Sbjct:    1 MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDELSYIGNCPWQE 51
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_STRTD (tRNA-specific 2-thiouridylase mnmA OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) GN=mnmA PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.665e-21
Identity = 42/84 (50.00%), Postives = 51/84 (60.71%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVV 522
            C   ED K   AV DQ+D+P   V+   EYW  V  Y + EY   RTPNPDV+CN  IKF  F+D   ++  DYVA+GHYA VV
Sbjct:   50 CTATEDYKDVAAVADQIDIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAMTLGADYVATGHYAQVV 133

HSP 2 Score: 35.4242 bits (80), Expect = 2.665e-21
Identity = 19/43 (44.19%), Postives = 25/43 (58.14%), Query Frame = 2 
Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQTSRTFGL 271
            +V V +SGGVDSSV   LL   G+  I  ++K W  T   FG+
Sbjct:    8 RVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDTDE-FGV 49
[Close]
BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_STRT2 (tRNA-specific 2-thiouridylase mnmA OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) GN=mnmA PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.665e-21
Identity = 42/84 (50.00%), Postives = 51/84 (60.71%), Query Frame = 1 
Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVV 522
            C   ED K   AV DQ+D+P   V+   EYW  V  Y + EY   RTPNPDV+CN  IKF  F+D   ++  DYVA+GHYA VV
Sbjct:   50 CTATEDYKDVAAVADQIDIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAMTLGADYVATGHYAQVV 133

HSP 2 Score: 35.4242 bits (80), Expect = 2.665e-21
Identity = 19/43 (44.19%), Postives = 25/43 (58.14%), Query Frame = 2 
Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQTSRTFGL 271
            +V V +SGGVDSSV   LL   G+  I  ++K W  T   FG+
Sbjct:    8 RVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDTDE-FGV 49
The following BLAST results are available for this feature:
BLAST of CX672009 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 471
Match NameE-valueIdentityDescription
MNMA_BORT92.844e-2447.25tRNA-specific 2-thiouridylase mnmA OS=Borrelia tur... [more]
MNMA_BORHD3.701e-2450.00tRNA-specific 2-thiouridylase mnmA OS=Borrelia her... [more]
MNMA_BORBZ2.338e-2345.78tRNA-specific 2-thiouridylase mnmA OS=Borrelia bur... [more]
MNMA_BORBU2.338e-2345.78tRNA-specific 2-thiouridylase mnmA OS=Borrelia bur... [more]
MNMA_BORAP3.252e-2244.58tRNA-specific 2-thiouridylase mnmA OS=Borrelia afz... [more]
MNMA_BORDL3.252e-2244.05tRNA-specific 2-thiouridylase mnmA OS=Borrelia dut... [more]
MNMA_BORRA9.317e-2242.86tRNA-specific 2-thiouridylase mnmA OS=Borrelia rec... [more]
MNMA_BORGA1.212e-2143.37tRNA-specific 2-thiouridylase mnmA OS=Borrelia gar... [more]
MNMA_STRTD2.665e-2150.00tRNA-specific 2-thiouridylase mnmA OS=Streptococcu... [more]
MNMA_STRT22.665e-2150.00tRNA-specific 2-thiouridylase mnmA OS=Streptococcu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS10_14D10_g Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10 Citrus sinensis cDNA clone UCRCS10-14D10-G20-5.g, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX672009 ID=CX672009; Name=CX672009; organism=Citrus sinensis; type=EST; length=833bp
CAAAAACTAATCTTCCTTAACTCTTGTTCTCTGTCAACATCAACCCAGCT
CACACTCTTTACAACACAATCCTAATTCGAATGTTAGTGTTGATTTTGAA
CCCCACTATCTCTCTTGCTGCATGCCCCATCGAAAGGGCCTTAAGGTTGC
TGTTCTTTTGAGCGGCGGCGTTGATAGCAGTGTTGCACTTAGACTTCTTA
ATGCTGCTGGTCATTCTTGCATAGCCTTTTACCTCAAAATTTGGTTTCAA
ACTTCGAGAACTTTTGGACTGAATGCCCATTGGAAGAAGATATGAAGTAT
GCAAAAGCTGTTTATGATCAGGTTGATGTACCACTAGAAGTGCATTTAAC
AGATGAATACTGGAAAAATGTGGTATCCTACATTATTGAAGAATATTGAT
GCGACCGTACACCCAACCCAGATGTCCTTTGTAATACAAGAATAAAATTT
GGTGTGTTCATGGATGCCATCAGCAGTATGGAATTTGATTACGTTGCTTC
TGGGCATTATGCCAATGTTGTCCACTCATCTGCGGATCATAAGGATAAGC
CTTCTGTGTAGAATTATCAAAAGACAAGTTGCTTATCCTGGATAAACATT
TGTGCTTTCATATTTCTCTCCTGTATTGGTGACTTGATTGTTTCCATTTG
GCATGTTTTTCTAAGGAATTCTGCTGAAGCTTTTGCTTTTATAAATGCTC
TTATTAGATATTATGACCATTATTCACATAAAAAATAGTGATTGTCACTT
AAATATGAAAAGCCCAGAGGCACCATGAATTCATCCCCAATTTGTTTATC
CTTTTCAAATCTCTTTTATCATAATCTATGGCC
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