EG358296

Overview
NameEG358296
Unique NameEG358296
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length597
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3887 contig Csv1_Contig3887:152..748. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL2_PEA (Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=PAL2 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 2.920e-61
Identity = 112/141 (79.43%), Postives = 122/141 (86.52%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VFSY DDPCSATYPLM+KLRQVLVDHAL N E  KN N SIF KI  FE+ELKTLLPKEVESAR A+E+GN  I N+IKECRSYPLY+FVREELG   LTGEK ISPGEECDK+FTAICQGKIIDPLLECL +W+G+PLPI
Sbjct:  583 VFSYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLNTSIFQKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYKFVREELGTSLLTGEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPI 723
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PALY_MALDO (Phenylalanine ammonia-lyase (Fragment) OS=Malus domestica GN=PAL PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 1.110e-60
Identity = 110/141 (78.01%), Postives = 122/141 (86.52%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+Y DDPCSATYPLM+KLR+VL++HALTN E  KNA+ SIF KIGAFEEELK LLPKEVESARSA E GN  +PNRI ECRSYPLY+FVREELG  YLTGEK  SPGEECDKVF AICQGKIIDP+L CL+ W+G+PLPI
Sbjct:   94 VFAYIDDPCSATYPLMQKLREVLIEHALTNGESEKNASTSIFQKIGAFEEELKALLPKEVESARSAIEGGNAAVPNRIAECRSYPLYKFVREELGGEYLTGEKVRSPGEECDKVFQAICQGKIIDPILGCLEGWNGAPLPI 234
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL2_POPKI (Phenylalanine ammonia-lyase G2B OS=Populus kitakamiensis GN=PALG2B PE=3 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.472e-60
Identity = 112/141 (79.43%), Postives = 122/141 (86.52%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VFSY DDPCSATYPLM+KLRQVLV+HAL N E ++N+  SIF KIG+FEEELKTLLPKEVESAR   E+GN  IPNRIKECRSYPLY+FVREELG   LTGEK  SPGEE DKVFTAIC GK+IDPLLECLKEWDG+PLPI
Sbjct:  569 VFSYIDDPCSATYPLMQKLRQVLVEHALVNGEKVRNSTTSIFQKIGSFEEELKTLLPKEVESARLEVENGNPAIPNRIKECRSYPLYKFVREELGTSLLTGEKVKSPGEEFDKVFTAICAGKLIDPLLECLKEWDGAPLPI 709
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL1_PRUAV (Phenylalanine ammonia-lyase 1 OS=Prunus avium GN=PAL1 PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 3.229e-60
Identity = 109/141 (77.30%), Postives = 126/141 (89.36%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+Y DDPCSATYPLM+KLRQVLV+HALTN E+ KNA+ SIF KI AFEEELK LLPKEV+SAR+A +SG+  +PNRI ECRSYPLY+FVREELGA YLTGEK  SPGEECDKVFTAIC+GKIIDP+L+CL+ W+G+PLPI
Sbjct:  576 VFAYIDDPCSATYPLMQKLRQVLVEHALTNGENEKNASTSIFQKIVAFEEELKVLLPKEVDSARAALDSGSAGVPNRITECRSYPLYKFVREELGAEYLTGEKVRSPGEECDKVFTAICEGKIIDPILDCLEGWNGAPLPI 716
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL1_PEA (Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=PAL1 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.227e-59
Identity = 108/141 (76.60%), Postives = 121/141 (85.82%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+Y DDPCSATYPLM+KLRQVLVDHAL N E  KN N SIF KI  FE+ELKTLLPKEVES R+A+ESGN  +PN+I  CRSYPLYRFVR+ELG   LTGEK ISPGEECDK+FTAICQGKIIDPLL+CL +W+G+PLPI
Sbjct:  582 VFAYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLNTSIFQKIATFEDELKTLLPKEVESTRAAYESGNPTVPNKINGCRSYPLYRFVRQELGTGLLTGEKVISPGEECDKLFTAICQGKIIDPLLQCLGDWNGAPLPI 722
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL1_TOBAC (Phenylalanine ammonia-lyase OS=Nicotiana tabacum GN=TPA1 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.733e-59
Identity = 107/141 (75.89%), Postives = 121/141 (85.82%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            +F+YADDPCS  YPLM+KLRQVLVDHA+ N E  KN N+SIF KIGAFE+ELK +LPKEVESAR+A ESGN  IPNRI ECRSYPLYRFVR+ELG   LTGEK  SPGEECDKVFTA+C G+IIDP+LECLK W+G+PLPI
Sbjct:  574 LFAYADDPCSCNYPLMQKLRQVLVDHAMNNGESEKNVNSSIFQKIGAFEDELKAVLPKEVESARAALESGNPAIPNRITECRSYPLYRFVRKELGTELLTGEKVRSPGEECDKVFTAMCNGQIIDPMLECLKSWNGAPLPI 714
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL2_ARATH (Phenylalanine ammonia-lyase 2 OS=Arabidopsis thaliana GN=PAL2 PE=1 SV=2)

HSP 1 Score: 227.639 bits (579), Expect = 3.570e-59
Identity = 105/141 (74.47%), Postives = 125/141 (88.65%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+Y DDPCSATYPLM++LRQV+VDHAL+N E  KNA  SIF KIGAFEEELK +LPKEVE+AR+A+ +G   IPNRIKECRSYPLYRFVREELG + LTGEK +SPGEE DKVFTA+C+GK+IDPL++CLKEW+G+P+PI
Sbjct:  576 VFTYVDDPCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQKIGAFEEELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEEFDKVFTAMCEGKLIDPLMDCLKEWNGAPIPI 716
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PALY_MEDSA (Phenylalanine ammonia-lyase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 4.663e-59
Identity = 109/141 (77.30%), Postives = 120/141 (85.11%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+Y DDPCSATYPL +KLRQVLVDHAL N E  KN N SIF KI  FEEELKTLLPKEVESAR+A+ESGN  IPN+I  CRSYPLY+FVREELG   LTGE  ISPGEECDK+F+A+CQGKIIDPLLECL EW+G+PLPI
Sbjct:  584 VFAYIDDPCSATYPLSQKLRQVLVDHALVNGESEKNFNTSIFQKIATFEEELKTLLPKEVESARTAYESGNPTIPNKINGCRSYPLYKFVREELGTGLLTGENVISPGEECDKLFSAMCQGKIIDPLLECLGEWNGAPLPI 724
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PAL1_IPOBA (Phenylalanine ammonia-lyase OS=Ipomoea batatas GN=PAL PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 4.663e-59
Identity = 108/141 (76.60%), Postives = 119/141 (84.40%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VF+YADDPCSA YPLM+KLRQ LVDHAL N E+ KN   SIFLK+ AFE+ELK +LPKEVE+AR A ESGN  IPNRIKECRSYPLY+FVRE LG   LTGEK  SPGEECDKVFTA+C+G IIDPLLECLK WDG+PLPI
Sbjct:  566 VFAYADDPCSANYPLMQKLRQALVDHALQNGENEKNTGTSIFLKVAAFEDELKAVLPKEVEAARIAVESGNPAIPNRIKECRSYPLYKFVREGLGTELLTGEKVRSPGEECDKVFTAMCEGSIIDPLLECLKSWDGAPLPI 706
[Close]
BLAST of EG358296 vs. ExPASy Swiss-Prot
Match: PALY_TRISU (Phenylalanine ammonia-lyase OS=Trifolium subterraneum GN=PAL1 PE=3 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 6.090e-59
Identity = 108/141 (76.60%), Postives = 121/141 (85.82%), Query Frame = 3 
Query:    3 VFSYADDPCSATYPLMRKLRQVLVDHALTNNEDLKNANASIFLKIGAFEEELKTLLPKEVESARSAFESGNLEIPNRIKECRSYPLYRFVREELGARYLTGEKAISPGEECDKVFTAICQGKIIDPLLECLKEWDGSPLPI 425
            VFSY DDPCSATYPL +KLRQVLVDHAL N E  KN+N SIF KI  FEEELKTLLPKEVESAR+A+E+GN  I N+I  CRSYPLY+FVREELG   LTGE+ ISPGEECDK+FTA+CQGKIIDPLL+CL EW+G+PLPI
Sbjct:  584 VFSYIDDPCSATYPLAQKLRQVLVDHALVNGESEKNSNTSIFQKIATFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSLLTGERVISPGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPI 724
The following BLAST results are available for this feature:
BLAST of EG358296 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 47
Match NameE-valueIdentityDescription
PAL2_PEA2.920e-6179.43Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=... [more]
PALY_MALDO1.110e-6078.01Phenylalanine ammonia-lyase (Fragment) OS=Malus do... [more]
PAL2_POPKI2.472e-6079.43Phenylalanine ammonia-lyase G2B OS=Populus kitakam... [more]
PAL1_PRUAV3.229e-6077.30Phenylalanine ammonia-lyase 1 OS=Prunus avium GN=P... [more]
PAL1_PEA1.227e-5976.60Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=... [more]
PAL1_TOBAC2.733e-5975.89Phenylalanine ammonia-lyase OS=Nicotiana tabacum G... [more]
PAL2_ARATH3.570e-5974.47Phenylalanine ammonia-lyase 2 OS=Arabidopsis thali... [more]
PALY_MEDSA4.663e-5977.30Phenylalanine ammonia-lyase OS=Medicago sativa PE=... [more]
PAL1_IPOBA4.663e-5976.60Phenylalanine ammonia-lyase OS=Ipomoea batatas GN=... [more]
PALY_TRISU6.090e-5976.60Phenylalanine ammonia-lyase OS=Trifolium subterran... [more]

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Properties
Property NameValue
Genbank description14a8 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl0014a8, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358296 ID=EG358296; Name=EG358296; organism=Citrus sinensis; type=EST; length=597bp
ACGTTTTTTCATACGCTGATGATCCCTGCAGCGCAACTTACCCGTTGATG
CGGAAACTGAGACAAGTCCTTGTTGATCATGCATTGACAAACAATGAAGA
TTTGAAGAATGCAAATGCTTCAATCTTCTTAAAGATAGGTGCTTTCGAGG
AGGAGTTGAAGACCCTTTTGCCGAAAGAAGTTGAGAGTGCTAGAAGTGCT
TTTGAGAGTGGTAATTTGGAAATACCAAACAGGATTAAGGAGTGCAGGTC
CTACCCATTGTATAGGTTTGTAAGGGAAGAGTTGGGGGCTCGGTATCTGA
CAGGAGAGAAGGCGATATCACCAGGCGAGGAATGTGATAAGGTGTTTACA
GCGATTTGTCAGGGAAAAATAATTGATCCACTGCTTGAATGTCTTAAAGA
ATGGGATGGGAGTCCTCTTCCAATTTGTTAATGCTTTCTTTTATGCCTTT
TTTTTTATATATATTTTAATGTCGCTTGGAAGGTTAACAAATATAATTCT
TCTACTGTGAGGATGATACTGTCTCTATGCTTTCACTTTGTGTTCTTAAT
TCTATTTTTCCCCCTTCTTAAAGTCTGTAATTTTGGTGAAATTTATG
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