CV713031

Overview
NameCV713031
Unique NameCV713031
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length788
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: LEGB_GOSHI (Legumin B OS=Gossypium hirsutum GN=LEGB PE=2 SV=1)

HSP 1 Score: 257.299 bits (656), Expect = 6.991e-68
Identity = 121/182 (66.48%), Postives = 150/182 (82.42%), Query Frame = -1
Query:  159 NGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTVFTPGPRSQ 704
            NG+EET C+M+LKH     S AD +NPRGGR+TTVN FNLPIL+Y+QLSAE+G LY NA+ APHWN+NAHS+VYITRGNGR+QIV+ENGE +FD Q+  GQ+I VPQ  AVVK+A  RG EWI+FKTN  AK SQ+AGR S++RGLP+DV+ NSF +SR+EA RLK+NRQE++VF+P   SQ
Sbjct:  335 NGLEETFCSMRLKHR-TPASSADVFNPRGGRITTVNSFNLPILQYLQLSAERGVLYNNAIYAPHWNMNAHSIVYITRGNGRIQIVSENGEAIFDEQVERGQVITVPQNHAVVKKAGRRGFEWIAFKTNANAKISQIAGRVSIMRGLPVDVLANSFGISREEAMRLKHNRQEVSVFSPRQGSQ 515          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: 11SB_CUCMA (11S globulin subunit beta OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 7.487e-62
Identity = 115/213 (53.99%), Postives = 159/213 (74.65%), Query Frame = -1
Query:  159 RVEEELRVLSPQRG---RXXXXXXXXQGSGDNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTVFTPGPRSQ 788
            +V+E+  VL P++    R        +   +NG+EETICT++LK NI     AD +NPRGGR++T N   LPILR ++LSAE+G LY NAM+APH+ +N+HSV+Y TRGN R+Q+V   G++VFDG++REGQ++++PQ F V+KRAS+RG EWI+FKTND A T+ LAGR S +R LPL V+ N +++SR+EAQRLKY +QE+ V +PG RSQ
Sbjct:  265 QVDEDFEVLLPEKDEEERSRGRYIESESESENGLEETICTLRLKQNIGRSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHYTVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAFKTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPG-RSQ 476          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: GLUA1_ORYSJ (Glutelin type-A 1 OS=Oryza sativa subsp. japonica GN=GLUA1 PE=1 SV=2)

HSP 1 Score: 217.624 bits (553), Expect = 6.139e-56
Identity = 103/205 (50.24%), Postives = 150/205 (73.17%), Query Frame = -1
Query:  174 EEELRVLSPQRGRXXXXXXXXQGSG-DNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNR-QELTVFTP 782
            +E+ +V S +R +         GSG  NG++ET CT++++ NI++P+ AD YNPR GRVT +N  N PIL  +Q+SA K NLYQNA+++P WN+NAHSVVYIT+G  R+Q+V  NG+ VF+G++R GQL+++PQ +AVVK+A   G  +I+FKTN  +  S +AG++S+ R LP DV+ N++++SR+EAQRLK+NR  E   FTP
Sbjct:  279 QEQGQVQSRERYQEGQYQQSQYGSGCSNGLDETFCTLRVRQNIDNPNRADTYNPRAGRVTNLNTQNFPILSLVQMSAVKVNLYQNALLSPFWNINAHSVVYITQGRARVQVVNNNGKTVFNGELRRGQLLIIPQHYAVVKKAQREGCAYIAFKTNPNSMVSHIAGKSSIFRALPNDVLANAYRISREEAQRLKHNRGDEFGAFTP 483          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: 13S1_FAGES (13S globulin seed storage protein 1 OS=Fagopyrum esculentum GN=FA02 PE=2 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 5.197e-55
Identity = 99/182 (54.40%), Postives = 133/182 (73.08%), Query Frame = -1
Query:  174 GSG-DNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTVFTP 716
            GSG  NG+E+  C +K K N+N PS AD +NPR GR+ TVN  NLPIL +IQLSA+   LY+NA++ P WNLNAHS +Y+TRG GR+Q+V + G +VFD  ++ GQ++VVPQGFAVV +A   GLEW+  K +D A TS +AG+ SV+R +P++V+ NS+ +S  EA RLK  RQE+ VF P
Sbjct:  372 GSGRSNGLEQAFCNLKFKQNVNRPSRADVFNPRAGRINTVNSNNLPILEFIQLSAQHVVLYKNAILGPRWNLNAHSALYVTRGEGRVQVVGDEGRSVFDDNVQRGQILVVPQGFAVVLKAGREGLEWVELKNDDNAITSPIAGKTSVLRAIPVEVLANSYDISTKEAFRLKNGRQEVEVFLP 553          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: GLUA2_ORYSJ (Glutelin type-A 2 OS=Oryza sativa subsp. japonica GN=GLUA2 PE=1 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 6.787e-55
Identity = 98/183 (53.55%), Postives = 140/183 (76.50%), Query Frame = -1
Query:  174 GSG-DNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNR-QELTVFTP 716
            GSG  NG++ET CTM+++ NI++P+ AD YNPR GRVT +N  N PIL  +Q+SA K NLYQNA+++P WN+NAHS+VYIT+G  ++Q+V  NG+ VF+G++R GQL++VPQ + VVK+A   G  +I+FKTN  +  S +AG++S+ R LP DV+ N++++SR+EAQRLK+NR  E   FTP
Sbjct:  301 GSGCPNGLDETFCTMRVRQNIDNPNRADTYNPRAGRVTNLNSQNFPILNLVQMSAVKVNLYQNALLSPFWNINAHSIVYITQGRAQVQVVNNNGKTVFNGELRRGQLLIVPQHYVVVKKAQREGCAYIAFKTNPNSMVSHIAGKSSIFRALPTDVLANAYRISREEAQRLKHNRGDEFGAFTP 483          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: 13SB_FAGES (13S globulin basic chain OS=Fagopyrum esculentum PE=1 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.975e-54
Identity = 93/170 (54.71%), Postives = 129/170 (75.88%), Query Frame = -1
Query:  192 GIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQE 701
            GI+E +CTMKL+ NI  P  AD YNP+ GR+TT N   LP LR +Q+SAE+G LY N + APHWN+NAHS +Y+TRGN ++Q+V + G  VFD ++++GQLI+VPQ FAV+K+A N+G E+++FKTND A  + L GR S  R +P +V+++SFQ+S +EA+ LKY RQE
Sbjct:    1 GIDENVCTMKLRENIKSPQEADFYNPKAGRITTANSQKLPALRSLQMSAERGFLYSNGIYAPHWNINAHSALYVTRGNAKVQVVGDEGNKVFDDEVKQGQLIIVPQYFAVIKKAGNQGFEYVAFKTNDNAMINPLVGRLSAFRAIPEEVLRSSFQISSEEAEELKYGRQE 170          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: GLUA3_ORYSJ (Glutelin type-A 3 OS=Oryza sativa subsp. japonica GN=GLUA3 PE=2 SV=2)

HSP 1 Score: 211.846 bits (538), Expect = 3.368e-54
Identity = 100/204 (49.02%), Postives = 146/204 (71.57%), Query Frame = -1
Query:  174 EEELRVLSPQRGRXXXXXXXXQGSGDNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNR-QELTVFTP 782
            +++ +V S   G+        QGS  NG++ET CTM+++ NI++P+ AD YNPR GR+T +N    PIL  +Q+SA K NLYQNA+++P WN+NAHSVVYIT+G  R+Q+V  NG+ VFDG++R GQL+++PQ   V+K+A   G  +I+ KTN  +  S +AG+ S+ R LP DV+ N++++SR+EA+RLK+NR  EL VFTP
Sbjct:  279 QQQEQVQSRDYGQTQYQQKQLQGSCSNGLDETFCTMRVRQNIDNPNLADTYNPRAGRITYLNGQKFPILNLVQMSAVKVNLYQNALLSPFWNINAHSVVYITQGRARVQVVNNNGKTVFDGELRRGQLLIIPQHHVVIKKAQREGCSYIALKTNPDSMVSHMAGKNSIFRALPDDVVANAYRISREEARRLKHNRGDELGVFTP 482          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: 13S3_FAGES (13S globulin seed storage protein 3 OS=Fagopyrum esculentum GN=FAGAG1 PE=1 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 4.399e-54
Identity = 97/182 (53.30%), Postives = 133/182 (73.08%), Query Frame = -1
Query:  174 GSG-DNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTVFTP 716
            GSG  NG+E+  C +K + N+N PS AD +NPR GR+ TV+  NLPIL +IQLSA+   LY+NA++ P WNLNAHS +Y+TRG GR+Q+V + G +VFD  ++ GQ++VVPQGFAVV +A   GLEW+  K +D A TS +AG+ SV+R +P++V+ NS+ +S  EA RLK  RQE+ VF P
Sbjct:  342 GSGRSNGLEQAFCNLKFRQNVNRPSRADVFNPRAGRINTVDSNNLPILEFIQLSAQHVVLYKNAILGPRWNLNAHSALYVTRGEGRVQVVGDEGRSVFDDNVQRGQILVVPQGFAVVLKAGREGLEWVELKNDDNAITSPIAGKTSVLRAIPVEVLANSYDISTKEAFRLKNGRQEVEVFRP 523          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: 12S1_ARATH (12S seed storage protein CRA1 OS=Arabidopsis thaliana GN=CRA1 PE=2 SV=2)

HSP 1 Score: 210.69 bits (535), Expect = 7.504e-54
Identity = 104/214 (48.60%), Postives = 150/214 (70.09%), Query Frame = -1
Query:  162 RVEEELRVLSP----QRGRXXXXXXXXQGSGDNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTV-FTPGPRS 788
            RV+    V+ P    QR +         G   NG+EETIC+ +   N++DPS AD Y P+ G ++T+N ++LPILR+I+LSA +G++ QNAM+ P WN NA++++Y+T G  ++QIV +NG  VFDGQ+ +GQLI VPQGF+VVKRA++   +W+ FKTN  A+ + LAGR SV+RGLPL+VI N FQ+S +EA+R+K+N  E T+  + GP S
Sbjct:  250 RVQGPFGVIRPPLRGQRPQEEEEEEGRHGRHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLETTLTHSSGPAS 463          
BLAST of CV713031 vs. ExPASy Swiss-Prot
Match: LEGA_GOSHI (Legumin A OS=Gossypium hirsutum GN=LEGA PE=2 SV=2)

HSP 1 Score: 210.305 bits (534), Expect = 9.800e-54
Identity = 99/214 (46.26%), Postives = 144/214 (67.29%), Query Frame = -1
Query:  174 RVEEELRVLSP---------QRGRXXXXXXXXQGSGDNGIEETICTMKLKHNINDPSGADAYNPRGGRVTTVNRFNLPILRYIQLSAEKGNLYQNAMMAPHWNLNAHSVVYITRGNGRMQIVAENGENVFDGQIREGQLIVVPQGFAVVKRASNRGLEWISFKTNDVAKTSQLAGRASVIRGLPLDVIQNSFQVSRDEAQRLKYNRQELTVFTP 788
            RV + L+V++P         +R +         GS DNG+EET C+M++K N+ DP  AD +NP+ GR++T+NRFNLPIL+ ++LSAE+G LY  A + P WN+NAH ++Y+ RG  R+Q+V  NG+ VFD  + +GQL+ VPQ FA +K+A N G EWISF TN  A  + +AG  S +R LP +V+  S+QVSR++A+R+K+N +    FTP
Sbjct:  287 RVRDRLQVVTPPRMEEEEREERQQEQRYRHTRGGSQDNGLEETFCSMRIKENLADPERADIFNPQAGRISTLNRFNLPILQRLELSAERGVLYNRAGLIPQWNVNAHKILYMLRGCARVQVVNHNGDAVFDDNVEQGQLLTVPQNFAFMKQAGNEGAEWISFFTNSEATNTPMAGSVSFMRALPEEVVAASYQVSREDARRIKFNNKNTFFFTP 500          
The following BLAST results are available for this feature:
BLAST of CV713031 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42
Match NameE-valueIdentityDescription
LEGB_GOSHI6.991e-6866.48Legumin B OS=Gossypium hirsutum GN=LEGB PE=2 SV=1[more]
11SB_CUCMA7.487e-6253.9911S globulin subunit beta OS=Cucurbita maxima PE=1... [more]
GLUA1_ORYSJ6.139e-5650.24Glutelin type-A 1 OS=Oryza sativa subsp. japonica ... [more]
13S1_FAGES5.197e-5554.4013S globulin seed storage protein 1 OS=Fagopyrum e... [more]
GLUA2_ORYSJ6.787e-5553.55Glutelin type-A 2 OS=Oryza sativa subsp. japonica ... [more]
13SB_FAGES1.975e-5454.7113S globulin basic chain OS=Fagopyrum esculentum P... [more]
GLUA3_ORYSJ3.368e-5449.02Glutelin type-A 3 OS=Oryza sativa subsp. japonica ... [more]
13S3_FAGES4.399e-5453.3013S globulin seed storage protein 3 OS=Fagopyrum e... [more]
12S1_ARATH7.504e-5448.6012S seed storage protein CRA1 OS=Arabidopsis thali... [more]
LEGA_GOSHI9.800e-5446.26Legumin A OS=Gossypium hirsutum GN=LEGA PE=2 SV=2[more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_0002D16_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0002D16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV713031 ID=CV713031; Name=CV713031; organism=Citrus sinensis; type=EST; length=788bp
TTTTCGGCAAAACATTTCAGTTTTCTTATTTCACCAGAACAAAACTTACA
GCAGCAGTACACTTTCGAGCCAACAGCAAGGACTTTATTCCTTATGTACA
TACACATATATAAACCCTTAATATAAGTTCATTACTTTGATCACGCACTA
ACTAATTACTGTGACCTAGGCCCTGGAGTAAAGACAGTCAGCTCCTGCCT
GTTGTACTTCAACCTCTGAGCTTCATCCCTCGACACTTGGAACGAGTTCT
GGATAACGTCCAACGGCAGCCCTCTAATCACCGAAGCCCTTCCGGCCAGC
TGGCTTGTCTTGGCGACGTCGTTTGTCTTGAATGATATCCACTCCAATCC
ACGGTTGCTTGCCCTCTTCACCACGGCGAAGCCCTGCGGGACAACGATCA
GCTGACCCTCCCGGATTTGACCGTCGAACACATTCTCCCCGTTCTCCGCC
ACAATTTGCATCCTGCCGTTGCCCCTTGTTATGTAGACTACGCTGTGGGC
ATTCAAGTTCCAGTGTGGCGCCATCATGGCATTCTGGTAGAGGTTCCCTT
TCTCAGCACTAAGCTGAATGTATCGAAGGATAGGAAGGTTGAATCTGTTG
ACGGTGGTGACGCGTCCGCCGCGGGGGTTGTACGCGTCAGCACCCGATGG
GTCATTGATGTTGTGCTTTAGCTTCATTGTACAGATAGTTTCCTCGATGC
CGTTGTCTCCGCTTCCTTGGAATTCTTGTTCTTGTTCTTGTTCTCTGCCT
CTTTGAGGTGACAGTACTCTCAGCTCTTCCTCGACTCT
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