CV712440

Overview
NameCV712440
Unique NameCV712440
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length780
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4678 contig Csv1_Contig4678:24..803. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX1_ARATH (L-ascorbate peroxidase 1, cytosolic OS=Arabidopsis thaliana GN=APX1 PE=1 SV=2)

HSP 1 Score: 397.512 bits (1020), Expect = 4.240e-110
Identity = 189/247 (76.52%), Postives = 211/247 (85.43%), Query Frame = 2
Query:   38 MTKNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            MTKNYPTVSEDYKKAVEKC+RKLRG IAEKNCAP+M+R+AWHSAGT+D +++TGGPFGTMR  AEQAH AN+G+ IA+RLL+P +EQFPTIS+AD +QLAGVV VEVTGGPDIPFHPGR+DK +PP EGRLPDA +G DHLR VF  QMGLSDKDIVALSG HTLGRCHK+RSGFEG WT NPLIFDNSYF ELL+GEK+GLLQL SDKALLDDPVFRPLVEK               H+KLSELGF
Sbjct:    1 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTMRFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGREDKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWTSNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKLSELGF 247          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX1_PEA (L-ascorbate peroxidase, cytosolic OS=Pisum sativum GN=APX1 PE=1 SV=2)

HSP 1 Score: 396.741 bits (1018), Expect = 7.232e-110
Identity = 191/247 (77.33%), Postives = 208/247 (84.21%), Query Frame = 2
Query:   38 MTKNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            M K+YPTVS DY+KA+EK KRKLRGFIAEK CAPL+LR+AWHSAGT+D KTKTGGPFGT++  AE AH ANNGLDIAVRLLEP KEQFP +SYAD YQLAGVV VE+TGGP++PFHPGR+DK EPP EGRLPDA +G+DHLR VFG  MGLSD+DIVALSGGHT+G  HKERSGFEGPWT NPLIFDNSYFTELLTGEKDGLLQLPSDKALL D VFRPLVEK               HLKLSELGF
Sbjct:    1 MGKSYPTVSPDYQKAIEKAKRKLRGFIAEKKCAPLILRLAWHSAGTFDSKTKTGGPFGTIKHQAELAHGANNGLDIAVRLLEPIKEQFPIVSYADFYQLAGVVAVEITGGPEVPFHPGREDKPEPPPEGRLPDATKGSDHLRDVFGKAMGLSDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLTGEKDGLLQLPSDKALLTDSVFRPLVEKYAADEDVFFADYAEAHLKLSELGF 247          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX1_ORYSJ (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. japonica GN=APX1 PE=1 SV=1)

HSP 1 Score: 390.193 bits (1001), Expect = 6.769e-108
Identity = 186/247 (75.30%), Postives = 209/247 (84.62%), Query Frame = 2
Query:   38 MTKNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            M KNYP VS +Y++AVEK ++KLR  IAEK+CAPLMLR+AWHSAGT+DV +KTGGPFGTM+  AE +H+AN GLDIAVR+LEP KE+ PTISYAD YQLAGVV VEV+GGP +PFHPGR+DK  PP EGRLPDA +G+DHLRQVFGAQMGLSD+DIVALSGGHTLGRCHKERSGFEGPWTRNPL FDNSYFTELL+G+K+GLLQLPSDKALL DP FRPLVEK               HLKLSELGF
Sbjct:    1 MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTMKTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGREDKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFRPLVEKYAADEKAFFEDYKEAHLKLSELGF 247          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX2_ORYSJ (L-ascorbate peroxidase 2, cytosolic OS=Oryza sativa subsp. japonica GN=APX2 PE=1 SV=1)

HSP 1 Score: 389.037 bits (998), Expect = 1.508e-107
Identity = 186/246 (75.61%), Postives = 207/246 (84.15%), Query Frame = 2
Query:   41 TKNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            +K+YPTVS++Y  AV K KRKLRG IAEKNCAPLMLR+AWHSAGT+DV ++TGGPFGTM+   EQ+H+AN GLDIAVRLL+P K+Q P +SYAD YQLAGVV VEVTGGP++PFHPGR DK EPP EGRLPDA QG+DHLRQVF AQMGLSDKDIVALSGGHTLGRCHKERSGFEG WT NPLIFDNSYFTEL++GEK+GLLQLPSDKAL+ DP FRPLVEK               HLKLSELGF
Sbjct:    3 SKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKNPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQDKPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTSNPLIFDNSYFTELVSGEKEGLLQLPSDKALMADPAFRPLVEKYAADEDAFFADYAEAHLKLSELGF 248          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX1_ORYSI (L-ascorbate peroxidase 1, cytosolic OS=Oryza sativa subsp. indica GN=APX1 PE=2 SV=1)

HSP 1 Score: 387.111 bits (993), Expect = 5.731e-107
Identity = 185/247 (74.90%), Postives = 208/247 (84.21%), Query Frame = 2
Query:   38 MTKNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            M KNYP VS +Y++AVEK ++KLR  IAEK+CAPLMLR+AWHSAGT+DV +KTGGPFGTM+  AE +H+AN GLDIAVR+LEP KE+ PTISYAD YQLAGVV VEV+GGP +PFHPGR+DK  PP EGRLPDA +G+DHLRQVFGAQMGLSD+DIVALSGGHTLGRCHKERSGFEGPWTRNPL FDNSYFTELL+G+K+GLLQLPSDKALL DP F PLVEK               HLKLSELGF
Sbjct:    1 MAKNYPVVSAEYQEAVEKARQKLRALIAEKSCAPLMLRLAWHSAGTFDVSSKTGGPFGTMKTPAELSHAANAGLDIAVRMLEPIKEEIPTISYADFYQLAGVVAVEVSGGPAVPFHPGREDKPAPPPEGRLPDATKGSDHLRQVFGAQMGLSDQDIVALSGGHTLGRCHKERSGFEGPWTRNPLQFDNSYFTELLSGDKEGLLQLPSDKALLSDPAFCPLVEKYAADEKAFFEDYKEAHLKLSELGF 247          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX2_ARATH (L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis thaliana GN=APX2 PE=2 SV=3)

HSP 1 Score: 377.867 bits (969), Expect = 3.477e-104
Identity = 182/245 (74.29%), Postives = 204/245 (83.27%), Query Frame = 2
Query:   44 KNYPTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            K+YP V E+YKKAV++CKRKLRG IAEK+CAP++LR+AWHSAGT+DVKTKTGGPFGT+R   E AH ANNGLDIAVRLL+P KE FP +SYAD YQLAGVV VE+TGGP+IPFHPGR DK EPP EGRLP A +G DHLR VFG +MGL+DKDIVALSGGHTLGRCHKERSGFEG WT NPLIFDNSYF E+L+GEK+GLLQLP+DKALLDDP+F P VEK               HLKLSELGF
Sbjct:    4 KSYPEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEGRLPQATKGVDHLRDVFG-RMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELGF 247          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX3_ARATH (L-ascorbate peroxidase 3, peroxisomal OS=Arabidopsis thaliana GN=APX3 PE=1 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 2.424e-81
Identity = 153/242 (63.22%), Postives = 175/242 (72.31%), Query Frame = 2
Query:   53 PTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            P V  +Y K + K +R+LR  IA KNCAP+MLR+AWH AGTYD ++KTGGP G++R   E  H AN+GL IA+ L E  K + P I+YADLYQLAGVV VEVTGGPDI F PGR D    P+EGRLPDAKQG  HLR VF  +MGLSDKDIVALSGGHTLGR H ERSGF+GPWT+ PL FDNSYF ELL GE +GLL+LP+DK LL+DP FR LVE                H KLSELGF
Sbjct:    4 PIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNEEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSNVCPKEGRLPDAKQGFQHLRDVF-YRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPLKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSELGF 244          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX3_ORYSJ (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. japonica GN=APX3 PE=2 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.471e-78
Identity = 145/242 (59.92%), Postives = 176/242 (72.73%), Query Frame = 2
Query:   53 PTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            P V  +Y   VE+ +R LR  IA K+CAP+MLR+AWH AGTYD  TKTGGP G++R   E +H+AN G+ IA+ LLEP K++ P I+YADLYQLAGVV VEVTGGP I + PGR D ++ P+EGRLPDAK+G  HLR+VF  +MGLSDKDIVALSGGHTLG+   ERSGF+G WT++PL FDNSYF ELL    +GLL+LP+DKAL++DP FR  VE                H KLSELGF
Sbjct:    5 PVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFPQEYSHAANAGIKIAIDLLEPMKQKHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSSDSPEEGRLPDAKKGAAHLREVF-YRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSELGF 245          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX3_ORYSI (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica GN=APX3 PE=2 SV=1)

HSP 1 Score: 292.738 bits (748), Expect = 1.471e-78
Identity = 145/242 (59.92%), Postives = 176/242 (72.73%), Query Frame = 2
Query:   53 PTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            P V  +Y   VE+ +R LR  IA K+CAP+MLR+AWH AGTYD  TKTGGP G++R   E +H+AN G+ IA+ LLEP K++ P I+YADLYQLAGVV VEVTGGP I + PGR D ++ P+EGRLPDAK+G  HLR+VF  +MGLSDKDIVALSGGHTLG+   ERSGF+G WT++PL FDNSYF ELL    +GLL+LP+DKAL++DP FR  VE                H KLSELGF
Sbjct:    5 PVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFPQEYSHAANAGIKIAIDLLEPMKQRHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSSDSPEEGRLPDAKKGAAHLREVF-YRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSELGF 245          
BLAST of CV712440 vs. ExPASy Swiss-Prot
Match: APX4_ORYSJ (Probable L-ascorbate peroxidase 4 OS=Oryza sativa subsp. japonica GN=APX4 PE=2 SV=1)

HSP 1 Score: 292.352 bits (747), Expect = 1.921e-78
Identity = 147/242 (60.74%), Postives = 173/242 (71.49%), Query Frame = 2
Query:   53 PTVSEDYKKAVEKCKRKLRGFIAEKNCAPLMLRIAWHSAGTYDVKTKTGGPFGTMRLAAEQAHSANNGLDIAVRLLEPFKEQFPTISYADLYQLAGVVGVEVTGGPDIPFHPGRDDKAEPPQEGRLPDAKQGNDHLRQVFGAQMGLSDKDIVALSGGHTLGRCHKERSGFEGPWTRNPLIFDNSYFTELLTGEKDGLLQLPSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHLKLSELGF 778
            P V  +Y + V++ +R LR  I+ K CAP+MLR+AWH AGTYDV TKTGG  G++R   E  H +N GL IA+ LLEP K + P I+YADLYQLAGVV VEVTGGP + F PGR D +  P+EGRLPDAK+G  HLR +F  +MGLSDKDIVALSGGHTLGR H ERSGFEG WT+ PL FDNSYF ELL GE +GLL+LP+DKALL+DP FR  V+                H KLSELGF
Sbjct:    4 PVVDAEYLRQVDRARRHLRALISSKGCAPIMLRLAWHDAGTYDVNTKTGGANGSIRYEEEYTHGSNAGLKIAIDLLEPIKAKSPKITYADLYQLAGVVAVEVTGGPTVEFIPGRRDSSVCPREGRLPDAKKGALHLRDIF-YRMGLSDKDIVALSGGHTLGRAHPERSGFEGAWTQEPLKFDNSYFLELLKGESEGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHKKLSELGF 244          
The following BLAST results are available for this feature:
BLAST of CV712440 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 90
Match NameE-valueIdentityDescription
APX1_ARATH4.240e-11076.52L-ascorbate peroxidase 1, cytosolic OS=Arabidopsis... [more]
APX1_PEA7.232e-11077.33L-ascorbate peroxidase, cytosolic OS=Pisum sativum... [more]
APX1_ORYSJ6.769e-10875.30L-ascorbate peroxidase 1, cytosolic OS=Oryza sativ... [more]
APX2_ORYSJ1.508e-10775.61L-ascorbate peroxidase 2, cytosolic OS=Oryza sativ... [more]
APX1_ORYSI5.731e-10774.90L-ascorbate peroxidase 1, cytosolic OS=Oryza sativ... [more]
APX2_ARATH3.477e-10474.29L-ascorbate peroxidase 2, cytosolic OS=Arabidopsis... [more]
APX3_ARATH2.424e-8163.22L-ascorbate peroxidase 3, peroxisomal OS=Arabidops... [more]
APX3_ORYSJ1.471e-7859.92Probable L-ascorbate peroxidase 3 OS=Oryza sativa ... [more]
APX3_ORYSI1.471e-7859.92Probable L-ascorbate peroxidase 3 OS=Oryza sativa ... [more]
APX4_ORYSJ1.921e-7860.74Probable L-ascorbate peroxidase 4 OS=Oryza sativa ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_0001G06_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001G06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV712440 ID=CV712440; Name=CV712440; organism=Citrus sinensis; type=EST; length=780bp
TGCGTCTCTCTTTCTCATTTCATTCAGCGTTGAAAAGATGACGAAGAATT
ACCCCACTGTTAGCGAGGATTACAAGAAGGCCGTGGAGAAATGCAAGAGG
AAGCTCAGAGGCTTCATCGCTGAGAAGAACTGCGCTCCACTCATGCTCCG
TATCGCGTGGCACTCAGCTGGTACATATGATGTGAAGACCAAGACCGGAG
GTCCATTCGGAACCATGAGGCTCGCTGCTGAGCAGGCACACTCAGCCAAC
AACGGCCTCGATATTGCCGTTAGGCTTTTGGAGCCGTTCAAGGAGCAGTT
CCCTACCATCTCCTACGCTGACCTTTATCAGCTTGCTGGTGTTGTTGGCG
TTGAGGTTACCGGTGGGCCCGATATCCCCTTCCACCCTGGAAGAGATGAC
AAGGCTGAGCCACCTCAAGAAGGCCGGCTTCCTGATGCTAAACAGGGTAA
TGATCACTTGAGGCAAGTCTTTGGTGCTCAGATGGGCCTCAGCGACAAGG
ATATTGTTGCTCTCTCTGGTGGCCACACCCTGGGAAGGTGCCACAAGGAG
AGGTCTGGATTTGAGGGACCATGGACCCGCAACCCTCTCATTTTTGACAA
TTCCTACTTCACGGAGCTCTTGACTGGTGAGAAGGATGGTCTTCTGCAAT
TGCCATCCGACAAGGCCCTTCTGGATGACCCTGTTTTCCGCCCTCTTGTT
GAGAAATATGCTGCGGATGAGGACGCTTTCTTCGCTGATTATGCTGAGGC
TCATCTCAAGCTCTCTGAGCTAGGATTTGC
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