CN189547

Overview
NameCN189547
Unique NameCN189547
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN189547_ssr5 microsatellite CN189547_ssr5:5..32. BLAST: Citrus ESTs to Prunus persica proteins V1
CN189547_ssr61 microsatellite CN189547_ssr61:61..72. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1286 contig Csv1_Contig1286:51..768. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 223.402 bits (568), Expect = 9.477e-58
Identity = 119/182 (65.38%), Postives = 132/182 (72.53%), Query Frame = 1 
Query:  169 MTLILRASPSCRVLTDVTRCTIRRSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            M L+ +A  S  + ++  +C    SG   +  N E  +MK+ C   + SSR S+K  Q ++LD             VAASPPTEDAVV  EPLTKEDLV YLASGCK KEKWRIGTEHEKFGFEFGTL PMKYEQIAELLN IAERFDWEKVMEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct:    1 MALMSQAGSSHCIYSEKMKCI---SGHSSITSNMEMLKMKDICFG-NISSRNSSKPMQGIYLDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAP 178
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 4.703e-57
Identity = 116/182 (63.74%), Postives = 132/182 (72.53%), Query Frame = 1 
Query:  169 MTLILRASPSCRVLTDVTRCTIRRSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            M L+ +A  S  + ++  RC    SG   +  N +  RM+E C     SSR +++  Q  +L++            VAASPPTEDAVV  EPLTKEDLVGYLASGCK KEKWRIGTEHEKFGFEFGTL PMKY+QIA+LLN IAERFDWEKVMEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct:    1 MALMSQAGSSHCIYSEKVRCI---SGHRSIINNMDMFRMREICFGVDISSRNASRRVQGNYLNHIGVGSRRGDLTIVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAP 179
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 198.364 bits (503), Expect = 3.265e-50
Identity = 105/159 (66.04%), Postives = 115/159 (72.33%), Query Frame = 1 
Query:  238 RSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            R+G   ++      RMKET VS           S+ L   +            VAASPPTE+AVV TEPLT+EDL+ YLASGCK KEKWRIGTEHEKFGFE  TL PMKY+QIAELLNSIAERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct:   21 RTGTKTVSGCVNVLRMKETYVS---------SYSRTLSTKSMLKRSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKSKEKWRIGTEHEKFGFEVNTLRPMKYDQIAELLNSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAP 170
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 197.978 bits (502), Expect = 4.264e-50
Identity = 109/172 (63.37%), Postives = 123/172 (71.51%), Query Frame = 1 
Query:  229 TIRRSGVCGLA----------KNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            T+  SGVC  A          +N +  RMKE   +F SS  YS  +S    L ++           VAASPPTE+AVV TEPLT+EDL+ YLASGCK K+K+RIGTEHEKFGFE  TL PMKY+QIAELLN IAERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct:   12 TVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE---AFGSS--YSRSLSTKSMLLHSVKRSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHEKFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAP 178
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 9.499e-50
Identity = 101/146 (69.18%), Postives = 115/146 (78.77%), Query Frame = 1 
Query:  283 MKETCVS--FSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            +++T +S  FS SS+ S    ++L    +           VAASPPTEDAVV TEPLTK+DL+ YLASGCK K+KWRIGTEHEKFGFE G+L PMKYEQI+ELLN IAERFDW+KVMEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct:   26 IRKTSISNTFSFSSKNSLSFKRILTSGGSRRFI-------VAASPPTEDAVVATEPLTKQDLIDYLASGCKTKDKWRIGTEHEKFGFELGSLRPMKYEQISELLNGIAERFDWDKVMEGDNIIGLKQGKQSISLEPGGQFELSGAP 164
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.202e-44
Identity = 83/106 (78.30%), Postives = 95/106 (89.62%), Query Frame = 1 
Query:  397 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            VAASPPTE+AV +TEPLTKEDLV YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAP
Sbjct:   43 VAASPPTEEAVQMTEPLTKEDLVAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAP 148
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 179.874 bits (455), Expect = 1.202e-44
Identity = 83/106 (78.30%), Postives = 95/106 (89.62%), Query Frame = 1 
Query:  397 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            VAASPPTE+AV +TEPLTKEDLV YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAP
Sbjct:   43 VAASPPTEEAVQMTEPLTKEDLVAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAP 148
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 2.677e-44
Identity = 82/106 (77.36%), Postives = 95/106 (89.62%), Query Frame = 1 
Query:  397 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            VAASPPTE+AV +TEPLTKEDL+ YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAP
Sbjct:   47 VAASPPTEEAVQMTEPLTKEDLMAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAP 152
[Close]
BLAST of CN189547 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 178.718 bits (452), Expect = 2.677e-44
Identity = 82/106 (77.36%), Postives = 95/106 (89.62%), Query Frame = 1 
Query:  397 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAP 714
            VAASPPTE+AV +TEPLTKEDL+ YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAP
Sbjct:   47 VAASPPTEEAVQMTEPLTKEDLMAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAP 152
The following BLAST results are available for this feature:
BLAST of CN189547 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 9
Match NameE-valueIdentityDescription
GSH1_TOBAC9.477e-5865.38Glutamate--cysteine ligase, chloroplastic OS=Nicot... [more]
GSH1_SOLLC4.703e-5763.74Glutamate--cysteine ligase, chloroplastic OS=Solan... [more]
GSH1_BRAJU3.265e-5066.04Glutamate--cysteine ligase, chloroplastic OS=Brass... [more]
GSH1_ARATH4.264e-5063.37Glutamate--cysteine ligase, chloroplastic OS=Arabi... [more]
GSH1_MEDTR9.499e-5069.18Glutamate--cysteine ligase, chloroplastic OS=Medic... [more]
GSH1A_ORYSJ1.202e-4478.30Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1A_ORYSI1.202e-4478.30Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1B_ORYSJ2.677e-4477.36Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1B_ORYSI2.677e-4477.36Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
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Properties
Property NameValue
Genbank descriptionUCRCS06_0002A15_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0002A15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189547 ID=CN189547; Name=CN189547; organism=Citrus sinensis; type=EST; length=716bp
GAGGCTCTCTCTCTCTCTCTCTCTCTCTCTCTGCATTCTGCAAGTGAGTC
TTCGCTGTCCTATATATATATAACTCAACGAGTGTTTTATCTTCTACGAA
ATTCGAAACCCTAGTTTTTAAGATTCCAATTGTGGTAATTCATTCAGCGT
TAACCAAGCGGGAACAGTATGACTCTCATTTTGCGGGCCAGTCCATCATG
TCGTGTATTGACTGATGTAACACGATGTACAATCAGACGAAGTGGGGTTT
GTGGCTTGGCAAAAAATGCTGAGGCATCTAGGATGAAGGAAACTTGTGTT
AGTTTCTCTTCCTCATCACGCTATTCTGCCAAGATATCACAAGTTTTACA
TCTAGACAATGCAGGGATAGGAATTCGAAGAGGACGTCGATCTATTGTTG
CAGCTAGTCCTCCCACGGAGGATGCTGTTGTTGTAACAGAGCCATTGACC
AAAGAGGATCTAGTTGGATACCTTGCCTCTGGGTGCAAGCCTAAGGAAAA
GTGGAGAATTGGTACAGAACATGAGAAGTTTGGTTTTGAGTTTGGAACTT
TACATCCTATGAAATACGAACAAATAGCCGAGTTGCTTAACAGCATTGCT
GAGAGGTTCGATTGGGAGAAAGTAATGGAAGGAGATTACATCATAGGACT
GAAACAAGGGAAACAAAGCATATCACTTGAACCTGGCGGTCAGTTTGAAC
TTAGTGGTGCACCCCT
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