CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRC_PEA (Glutathione reductase, cytosolic OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 410.609 bits (1054), Expect = 4.135e-114
Identity = 199/231 (86.15%), Postives = 220/231 (95.24%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKKILVYGAS+GGEL+DAR++GWE++E +DF+WKKLLQKKTDEI RLNGIYKRLLSNAGVKL+EGEGKI  PNEVEVTQLDGTKLSYSAKHILIATGSRAQR  IPGQEL ITSDEALSLEE PKRAV+LGGGYIAVEFASIWRGMGS+V+L+FRKELPLRGFDDEMRAVVARNLEGRGINLHPRT + +LIK+E+G+K ITDHGEE++ADVVLFATGR+P
Sbjct:   69 GGTCVIRGCVPKKILVYGASYGGELQDARNFGWELNENVDFNWKKLLQKKTDEINRLNGIYKRLLSNAGVKLFEGEGKIASPNEVEVTQLDGTKLSYSAKHILIATGSRAQRPNIPGQELGITSDEALSLEEFPKRAVILGGGYIAVEFASIWRGMGSSVNLVFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTNLAQLIKTEDGIKVITDHGEELIADVVLFATGRSP 299          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRP_SPIOL (Glutathione reductase, chloroplastic (Fragment) OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 407.142 bits (1045), Expect = 4.572e-113
Identity = 199/231 (86.15%), Postives = 217/231 (93.94%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKKILVYGASFGGELEDA++YGWE++EKIDF+WKKLLQKKTDEI+RLN IYKRLLSNAGVKLYEGEGKIVGPNEV+VTQLDGTKLSYSAKHILIATGSRAQR  IPGQELAITSDEALSLEE PKR V+LGGGYI+VEFASIWRGMG+ V+L FRKELPLRGFDDEMRA VARNLEGRG+N+HPRTT+ EL+K++ GV   TDHGEEI ADVVLFATGR+P
Sbjct:   60 GGTCVIRGCVPKKILVYGASFGGELEDAKNYGWELNEKIDFNWKKLLQKKTDEIIRLNNIYKRLLSNAGVKLYEGEGKIVGPNEVQVTQLDGTKLSYSAKHILIATGSRAQRPNIPGQELAITSDEALSLEEFPKRVVILGGGYISVEFASIWRGMGADVNLCFRKELPLRGFDDEMRAAVARNLEGRGVNVHPRTTLTELVKTDGGVVARTDHGEEIEADVVLFATGRSP 290          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRC_BRARP (Glutathione reductase, cytosolic OS=Brassica rapa subsp. pekinensis GN=GR1 PE=2 SV=2)

HSP 1 Score: 399.438 bits (1025), Expect = 9.533e-111
Identity = 192/231 (83.12%), Postives = 216/231 (93.51%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKKILVYGA++GGELEDAR+YGWE++  +DF+WKKLLQKKTDEILRLN IYKRLL+NA VKLYEGEG+IVGPNEVEV Q+DGTK+SY+AKHILIATGSRAQ+  IPG ELAITSDEALSLEE PKRA+VLGGGYIAVEFASIWRGMG+TVDL FRKELPLRGFDDEMRA+VARNLEGRGINLHP+T++ ELIK+++G+K I+ HGEE VADVVLFATGR P
Sbjct:   73 GGTCVIRGCVPKKILVYGATYGGELEDARNYGWEINGNVDFNWKKLLQKKTDEILRLNNIYKRLLANAAVKLYEGEGRIVGPNEVEVRQIDGTKISYTAKHILIATGSRAQKPNIPGHELAITSDEALSLEEFPKRAIVLGGGYIAVEFASIWRGMGATVDLFFRKELPLRGFDDEMRALVARNLEGRGINLHPQTSLAELIKTDDGIKVISSHGEEFVADVVLFATGRIP 303          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRC_ARATH (Glutathione reductase, cytosolic OS=Arabidopsis thaliana GN=At3g24170 PE=2 SV=1)

HSP 1 Score: 399.053 bits (1024), Expect = 1.245e-110
Identity = 189/231 (81.82%), Postives = 217/231 (93.94%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKKILVYGA++GGELEDA++YGWE++EK+DF WKKLLQKKTDEILRLN IYKRLL+NA VKLYEGEG++VGPNEVEV Q+DGTK+SY+AKHILIATGSRAQ+  IPG ELAITSDEALSLEE PKRA+VLGGGYIAVEFASIWRGMG+TVDL FRKELPLRGFDDEMRA+VARNLEGRG+NLHP+T++ +L K+++G+K I+ HGEE VADVVLFATGR+P
Sbjct:   70 GGTCVIRGCVPKKILVYGATYGGELEDAKNYGWEINEKVDFTWKKLLQKKTDEILRLNNIYKRLLANAAVKLYEGEGRVVGPNEVEVRQIDGTKISYTAKHILIATGSRAQKPNIPGHELAITSDEALSLEEFPKRAIVLGGGYIAVEFASIWRGMGATVDLFFRKELPLRGFDDEMRALVARNLEGRGVNLHPQTSLTQLTKTDQGIKVISSHGEEFVADVVLFATGRSP 300          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRC_ORYSJ (Glutathione reductase, cytosolic OS=Oryza sativa subsp. japonica GN=GRC2 PE=2 SV=2)

HSP 1 Score: 348.206 bits (892), Expect = 2.520e-95
Identity = 167/231 (72.29%), Postives = 199/231 (86.15%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKKILVYG+SF GE EDA+++GWE++  I+F+WK+LL+ KT EI+RLNG+Y+R+L N+GV + EG G +V  + VEVT+ DG+K  Y+AKHILIATGSRAQR  IPG+ELAITSDEALSLEELPKRAV+LGGGYIAVEFASIW+GMG+ VDL +RKELPLRGFDDEMR VVA NLEGRGI LHP T + EL K+ +G+K +TD GEEI+ADVVLFATGR P
Sbjct:   67 GGTCVIRGCVPKKILVYGSSFRGEFEDAKNFGWEINGDINFNWKRLLENKTQEIVRLNGVYQRILGNSGVTMIEGAGSLVDAHTVEVTKPDGSKQRYTAKHILIATGSRAQRVNIPGKELAITSDEALSLEELPKRAVILGGGYIAVEFASIWKGMGAHVDLFYRKELPLRGFDDEMRTVVASNLEGRGIRLHPGTNLSELSKTADGIKVVTDKGEEIIADVVLFATGRTP 297          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHR_PSEAE (Glutathione reductase OS=Pseudomonas aeruginosa GN=gor PE=3 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 4.071e-53
Identity = 116/232 (50.00%), Postives = 150/232 (64.66%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRIT-DHGEEIVADVVLFATGRAP 705
            GGTCV  GCVPKK+LVYGA F  + E AR+YGW   E   FDW  L+  K  EI RLNGIY+ LL N+GV L EG  +++  + VEV   DG +  +SAKHIL+ATG   Q   IPG+E AITS+EA  LE LP+R +V+GGGYIAVEFASI+ G+G+   LL+R++L LRGFD  +R  +   L  +G++L   + I  + K  +G    T   G  + AD V +ATGR P
Sbjct:   39 GGTCVNVGCVPKKLLVYGAHFSEDFEQARAYGWSAGEA-QFDWATLIGNKNREIQRLNGIYRNLLVNSGVTLLEGHARLLDAHSVEV---DGQR--FSAKHILVATGGWPQVPDIPGKEHAITSNEAFFLERLPRRVLVVGGGYIAVEFASIFNGLGAETTLLYRRDLFLRGFDRSVREHLRDELGKKGLDLQFNSDIARIDKQADGSLAATLKDGRVLEADCVFYATGRRP 264          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRP_PEA (Glutathione reductase, chloroplastic/mitochondrial OS=Pisum sativum GN=GR PE=2 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.310e-51
Identity = 111/232 (47.84%), Postives = 146/232 (62.93%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRI-TDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKK+LVY + F  E E++  +GW    +   DW  L+  K  E+ RL GIYK  L NAGVKL EG GKIV  + V+V   DG KL YSAKHIL++ G R     IPG+E AI SD AL L   P++  ++GGGYIA+EFA I+ G+ S V +  R++  LRGFD+E+R  VA N+  RGI  H   +   + K+ +G   + T+ G E     ++FATGR+P
Sbjct:  118 GGTCVIRGCVPKKLLVYASKFSHEFEESNGFGWRYDSEPKHDWSSLIANKNAELQRLTGIYKNTLKNAGVKLIEGRGKIVDAHTVDV---DG-KL-YSAKHILVSVGGRPFIPDIPGKEYAIDSDAALDLPSKPQKIAIVGGGYIALEFAGIFNGLKSEVHVFIRQKKVLRGFDEEIRDFVAENMALRGIEFHTEESPVAITKAADGSLSLKTNKGTEEGFSHIMFATGRSP 344          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRP_SOYBN (Glutathione reductase, chloroplastic OS=Glycine max GN=GR PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 1.109e-50
Identity = 107/232 (46.12%), Postives = 143/232 (61.64%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRI-TDHGEEIVADVVLFATGRAP 705
            GGTCVIRGCVPKK+LVY + F  E E++  +GW    +   DW   +  K  E+ RL GIYK +L+NAGVKL EG GK++ P+ V+V      KL YSAKHIL+A G R     IPG+ELAI SD AL L   P +  ++GGGYIA+EFA I+ G+ S V +  R++  LRGFD+E+R  V   +  RGI  H   + + + KS +G   + T+ G       ++FATGR P
Sbjct:  110 GGTCVIRGCVPKKLLVYASKFSHEFEESNGFGWRYDSEPKHDWSSFIANKNAELQRLTGIYKNILNNAGVKLIEGHGKMIDPHTVDV----NGKL-YSAKHILVAVGGRPFIPDIPGKELAIDSDAALDLPTKPVKIAIVGGGYIALEFAGIFNGLKSEVHVFIRQKKVLRGFDEEIRDFVEEQMSVRGIEFHTEESPQAITKSADGSFSLKTNKGTVDGFSHIMFATGRRP 336          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRP_TOBAC (Glutathione reductase, chloroplastic (Fragment) OS=Nicotiana tabacum GN=GOR PE=1 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 1.601e-49
Identity = 107/232 (46.12%), Postives = 143/232 (61.64%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRI-TDHGEEIVADVVLFATGRAP 705
            GGTCV+RGCVPKK+LVY + +  E E++  +GW    +  FDW  L+  K  E+ RL GIYK +L NAGV L EG GK+V P+ V+V   DG KL YSAK+ILI+ G R     IPG E AI SD AL L   P +  ++GGGYIA+EFA I+ G+ S V +  R++  LRGFD+E+R  V   +  RGI  H   + + ++KS +G   + T  G       ++FATGR P
Sbjct:  124 GGTCVLRGCVPKKLLVYASKYSHEFEESCGFGWNYDVEPRFDWSTLIANKNAELQRLTGIYKNILKNAGVTLIEGRGKVVDPHTVDV---DG-KL-YSAKNILISVGGRPFIPDIPGSEYAIDSDAALDLPTKPNKIAIVGGGYIALEFAGIFNGLKSEVHVFIRQKKVLRGFDEEIRDFVGEQMSLRGIEFHTEESPQAIVKSADGSLSLKTSRGTVEGFSHIMFATGRRP 350          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: GSHRP_ARATH (Glutathione reductase, chloroplastic OS=Arabidopsis thaliana GN=EMB2360 PE=2 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 1.770e-48
Identity = 102/232 (43.97%), Postives = 141/232 (60.78%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRI-TDHGEEIVADVVLFATGRAP 705
            GGTCV+RGCVPKK+LVY + +  E ED+  +GW+   +   DW  L+  K  E+ RL GIYK +LS A VKL EG GK++ P+ V+V   DG    Y+ ++ILIA G R     IPG+E AI SD AL L   PK+  ++GGGYIA+EFA I+ G+   V +  R++  LRGFD+++R  V   +  RGI  H   + + +IK+ +G   + T  G       V+FATGR P
Sbjct:  132 GGTCVLRGCVPKKLLVYASKYSHEFEDSHGFGWKYETEPSHDWTTLIANKNAELQRLTGIYKNILSKANVKLIEGRGKVIDPHTVDV---DGK--IYTTRNILIAVGGRPFIPDIPGKEFAIDSDAALDLPSKPKKIAIVGGGYIALEFAGIFNGLNCEVHVFIRQKKVLRGFDEDVRDFVGEQMSLRGIEFHTEESPEAIIKAGDGSFSLKTSKGTVEGFSHVMFATGRKP 358          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
GSHRC_PEA4.135e-11486.15Glutathione reductase, cytosolic OS=Pisum sativum ... [more]
GSHRP_SPIOL4.572e-11386.15Glutathione reductase, chloroplastic (Fragment) OS... [more]
GSHRC_BRARP9.533e-11183.12Glutathione reductase, cytosolic OS=Brassica rapa ... [more]
GSHRC_ARATH1.245e-11081.82Glutathione reductase, cytosolic OS=Arabidopsis th... [more]
GSHRC_ORYSJ2.520e-9572.29Glutathione reductase, cytosolic OS=Oryza sativa s... [more]
GSHR_PSEAE4.071e-5350.00Glutathione reductase OS=Pseudomonas aeruginosa GN... [more]
GSHRP_PEA1.310e-5147.84Glutathione reductase, chloroplastic/mitochondrial... [more]
GSHRP_SOYBN1.109e-5046.12Glutathione reductase, chloroplastic OS=Glycine ma... [more]
GSHRP_TOBAC1.601e-4946.12Glutathione reductase, chloroplastic (Fragment) OS... [more]
GSHRP_ARATH1.770e-4843.97Glutathione reductase, chloroplastic OS=Arabidopsi... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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