DY256666

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAON_SOLTU (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 466.077 bits (1198), Expect = 1.796e-130
Identity = 240/330 (72.73%), Postives = 263/330 (79.70%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDF--MGLREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGMQK 986
            LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMI+NWP+ QVDMIVLTDGSRILGLGDLGVQGIGIPIGKLD+YVAAAGINPQR+LPV LDVGTNNQKLLED LYLGLRQPRLEGEEYLSIVDEF+EAVHARWPKA+VQFEDFQ KWAFETL+RYRK+FCMFNDDIQ              RAQG  LTDFA+QKI             KMA+QA +RM G +   A   FFLLDK+GLITK+RK++DPAA PFAK   +   +GL+EGA L EVV+KVK             FHEE  + M++
Sbjct:  105 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIFNWPSTQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPVMLDVGTNNQKLLEDPLYLGLRQPRLEGEEYLSIVDEFVEAVHARWPKAVVQFEDFQAKWAFETLDRYRKKFCMFNDDIQGTAGVALAGLLGTVRAQGRPLTDFANQKIVVVGAGSAGLGVLKMALQAVSRMTGPS---ADPHFFLLDKNGLITKDRKDIDPAALPFAKAHHEIEGLGLQEGAGLAEVVKKVKPHVLLGLSGVGGIFHEEVLRAMKE 431          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAOM_SOLTU (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 383.259 bits (983), Expect = 1.531e-105
Identity = 193/309 (62.46%), Postives = 234/309 (75.73%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNND-AF--ARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDF--MGLREGASLLEVVRKVK 914
            LHDRNETLYY+VL++NI+++API+YTPTVGLVCQ YSGLFRRPRGMYFSA+D+GEMMSM+YNWPA QVDMIV+TDGSRILGLGDLG+QGIGI IGKLD+YVAAAGINPQR+LPV +DVGT+N+ LL+D LYLGL+  RL+GEEY+ ++DEFMEAV  RWP  IVQFEDFQ KWAF+ L+RYR  + MFNDDIQ              RAQG  + DF   KI               A +  ARM GN + AF  AR++F+++D  GLIT+ R+N+DP A PFA+   +    GL EGA+L EVVR+VK
Sbjct:  122 LHDRNETLYYKVLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTDNENLLKDPLYLGLQDHRLDGEEYIEVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDIQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARMLGNTEIAFESARSQFWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVVREVK 430          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAOM_AMAHP (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus PE=1 SV=1)

HSP 1 Score: 363.614 bits (932), Expect = 1.255e-99
Identity = 181/309 (58.58%), Postives = 230/309 (74.43%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAF---ARNKFFLLDKDGLITKERKNLDPAAAPFA--KDPGDFMGLREGASLLEVVRKVK 914
            LHDRNET++++VLI+NI+++API+ TPTVGLVCQ +SGL+RRPRGMYFS+ D+GEMMSM+YNWPA+QVDMIV+TDGSRILGLGDLGV GIG+ IGKLD+YVAAAGINPQR+LPV +DVGTNN+ LL++ LYLGL++ RL+GEEYL+++DEFMEAV  RWP  IVQFED Q KWA   L+RYR ++  FN D+Q              RAQG  + DF  QKI               A +  ARM GN+++    AR++F+++D  GLIT++R NLDP   PFA  ++     GL EGA L+EVVR+VK
Sbjct:  119 LHDRNETMFFKVLIENIEEYAPIVSTPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGVAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEDLLKNPLYLGLQKKRLDGEEYLAVMDEFMEAVFTRWPNVIVQFEDIQNKWALTLLQRYRHKYRTFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSSGVGVLNAARKTMARMLGNDESAFDRARSQFWVVDDKGLITEKRANLDPEVQPFAWKENEISLQGLNEGAKLVEVVRQVK 427          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAOH_NEOFR (Malic enzyme, hydrogenosomal OS=Neocallimastix frontalis PE=1 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 1.655e-67
Identity = 132/305 (43.28%), Postives = 189/305 (61.97%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQA-AARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFMGLREGASLLEVVRKVK 914
            L +RNETLYY+++++N  + APIIYTP VG  CQ +  +F + RGMYFS  D+G+M ++  NWP   VD+IV+TDGSRILGLGDLG  G+ IPIGKL +YV   GINP+ +LP+ LDVGTNN++LL D LYLG++ PRL+GEE+ + VDE++ A+  R+PKA++QFEDF M  A + L +Y+ + CMFNDDIQ              RA+G +  D   +               +  V    A  A   +A+A  +F++ D  GL+ K R +L P+   F +       +  G +  E+++K+K
Sbjct:  110 LQNRNETLYYKMILENFVELAPIIYTPVVGEACQKFHKIFTQTRGMYFSTADRGQMSAVAANWPYDDVDVIVVTDGSRILGLGDLGAGGMQIPIGKLTLYVCGGGINPRNVLPIVLDVGTNNKELLNDPLYLGMQHPRLQGEEFHAFVDEWVSAITDRFPKAVIQFEDFMMPNALDLLLKYKDQICMFNDDIQSTGAITLASVLATMRARGGTFADIKKETFLCLGAGSSGVGVCETIVDCIVAEGATREEAYA--QFYMFDHKGLLGKGRDDLLPSQQVFMRKE-----IEGGKTPAELLKKIK 407          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAOX_RAT (NADP-dependent malic enzyme OS=Rattus norvegicus GN=Me1 PE=1 SV=2)

HSP 1 Score: 245.358 bits (625), Expect = 4.983e-64
Identity = 137/330 (41.52%), Postives = 186/330 (56.36%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARW-PKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFMGLREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGMQKF 989
            L DRNE L+Y VL+ N++ F PI+YTPTVGL CQ YS  FR+PRG++ S  DKG + S++  WP   V  IV+TDG RILGLGDLG  G+GIP+GKL +Y A  G+NPQ+ LP+TLDVGT N++LL+D LY+GLR  R+ G EY + +DEFMEA  +++    ++QFEDF    AF  L +YR ++C FNDDIQ              R   ++    +DQ +              + V A  +  G +   AR K +L+D  GLI K R +L      FA +        E  +L  +V+K+K             F E+  K M  F
Sbjct:   78 LQDRNEKLFYSVLMSNVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALR---ITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEK-EGLSKEKARQKIWLVDSKGLIVKGRASLTEEKEVFAHEH------EEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAF 397          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAOX_MOUSE (NADP-dependent malic enzyme OS=Mus musculus GN=Me1 PE=1 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 4.983e-64
Identity = 136/330 (41.21%), Postives = 187/330 (56.67%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARW-PKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFMGLREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGMQKF 989
            L DRNE L+Y VL+ +++ F PI+YTPTVGL CQ YS  FR+PRG++ S  DKG + S++  WP   V  IV+TDG RILGLGDLG  G+GIP+GKL +Y A  G+NPQ+ LP+TLDVGT N++LL+D LY+GLR  R+ G EY + +DEFMEA  +++    ++QFEDF  + AF  L +YR ++C FNDDIQ              R   ++    +DQ +              + V A  +  G +   AR K +L+D  GLI K R +L      FA +        E  +L  +V+K+K             F E+  K M  F
Sbjct:   78 LQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALR---ITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEK-EGLSKENARKKIWLVDSKGLIVKGRASLTEEKEVFAHEH------EEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAF 397          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAO1_ERWT9 (NAD-dependent malic enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=sfcA PE=3 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 8.499e-64
Identity = 136/331 (41.09%), Postives = 187/331 (56.50%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFMG---LREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGMQK 986
            + D NETL+YR+L +++++  PIIYTPTVG  C+++S ++RR RG++ S  ++  +  M+ N   Q V +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV LDVGTNNQ+LL D LY+G R PR+ GEEY + V+EF++AV +RWP  ++QFEDF  K A   LERYR   C FNDDIQ              RA G  L    +QK+             +  + A  +  G +D  AR + F++D+ GL+T    NL    +   +      G     +  SLL+VVR  K             F EE  + M K
Sbjct:   80 IQDTNETLFYRLLDNHLEEMMPIIYTPTVGAACEHFSEIYRRARGLFISYPNRANIEDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRHPRITGEEYDNFVNEFIQAVKSRWPNVLLQFEDFAQKNAMPLLERYRDEVCCFNDDIQGTAAVTLGTLIAASRAAGGKL---CEQKVVFLGAGSAGCGIAEQII-AQMKSEGLSDEEARARVFMVDRFGLLTDRLPNLLNFQSRLVQKSDSLSGWDSANDSLSLLDVVRNAKPDIMIGVSGQPGLFSEEIVREMHK 406          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAO1_ACIAD (NAD-dependent malic enzyme OS=Acinetobacter sp. (strain ADP1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 1.110e-63
Identity = 135/326 (41.41%), Postives = 188/326 (57.67%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMA--GNNDAFARNKFFLLDKDGLITKERKNL---DPAAAPFAKDPGDFMGLREGASLLEVVRKVKXXXXXXXXXXXXXFHEE 965
            + D NETL+YR++ D++++  PIIYTPTVG  CQ +S ++RR RG++ S  D+  +  ++ N   + V +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LP+T+DVGTNNQ+LL D +Y+G RQPR+ G+EY   +D+ +  +  RWP A++QFEDF  K A   L +YR +FC FNDDIQ              RA G  L    DQ I              +A Q  A+M   G  DA AR + +++D+ GLIT+ + NL       A  A+   D+  + E  SLL+VV+  K             F EE
Sbjct:   81 IQDTNETLFYRLINDHLEEMMPIIYTPTVGEACQRFSDIYRRHRGIFISYPDREHIDDILQNVSKRNVKVIVITDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPITIDVGTNNQQLLNDPIYMGWRQPRISGDEYYEFIDQVLTGIRRRWPHALIQFEDFAQKNAMPLLTKYRDKFCCFNDDIQGTAAVSVGSLIAASRAAGKQL---KDQTITFLGAGSAGCG---IAEQIVAQMVAEGLTDAQARARVYMVDRFGLITENQPNLLDFQRKLAQKAEVVSDWGNVEEVISLLDVVKNAKPTVLIGVSGQPGLFTEE 400          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPW (NAD-dependent malic enzyme OS=Shewanella piezotolerans (strain WP3 / JCM 13877) GN=sfcA PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.893e-63
Identity = 135/329 (41.03%), Postives = 185/329 (56.23%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFM---GLREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGM 980
            + D NETLYYR++ ++I +  PIIYTPTVG+ C+ +S  +RR RG++ S  +K  +  ++ N   Q+V +IV+TDG RILGLGD G+ G+GIPIGKL +Y +  GI+P   LP+TLDVGT+N  LLED +Y+G R PR+ GEEY   V+ FM+AVH RWP A++QFEDF  K A   LERY+ ++C FNDDIQ              +A G   T    Q+I             + A+ A     G  ++ AR + F++D+ G++     NL P     A+D  D        E  SLL+VV   K             F EE  K M
Sbjct:   77 IQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKDYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPITLDVGTDNPHLLEDPMYMGWRNPRIGGEEYTEFVEAFMQAVHRRWPDALIQFEDFAQKNAMPLLERYKDQYCCFNDDIQGTAAVTVGSLLAACKAAG---TQLCQQRITFLGAGSAGCGIAE-AIVAQMVSEGIAESQARKQVFMVDRWGMLQSNMPNLLPFQQKLAQDCDDITNWDNFSENISLLDVVNNAKPTILIGVSGAPGLFSEEIIKAM 401          

BLAST of DY256666 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPA (NAD-dependent malic enzyme OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 242.662 bits (618), Expect = 3.230e-63
Identity = 133/329 (40.43%), Postives = 189/329 (57.45%), Query Frame = 3

Query:    3 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVTLDVGTNNQKLLEDRLYLGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAFETLERYRKRFCMFNDDIQXXXXXXXXXXXXXXRAQGLSLTDFADQKIXXXXXXXXXXXXXKMAVQAAARMAGNNDAFARNKFFLLDKDGLITKERKNLDPAAAPFAKDPGDFM---GLREGASLLEVVRKVKXXXXXXXXXXXXXFHEEGAKGM 980
            + D NETLYYR++ ++I +  PIIYTPTVG+ C+ +S  +RR RG++ S  +K  +  ++ N   Q+V +IV+TDG RILGLGD G+ G+GIPIGKL +Y +  GI+P   LP+TLDVGT+N  LLED +Y+G+R PR+ GEEY   V+ FM+AVH RWP A++QFEDF  K A   LERY+ ++C FNDDIQ              +A   + T  ++Q+I             + A+ A     G ++A AR + F++D+ G++     NL P     A++  D        +  SLL+VV   K             F EE  K M
Sbjct:   77 IQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKDYRRNRGLFISYANKDRIDDILNNSTRQKVKIIVVTDGERILGLGDQGIGGMGIPIGKLSLYTSCGGISPAYTLPITLDVGTDNPHLLEDPMYMGMRSPRIGGEEYTEFVEAFMQAVHRRWPDALIQFEDFAQKNAMPLLERYKDQYCCFNDDIQGTAAVTVGSLLAACKA---AKTQLSEQRITFLGAGSAGCGIAE-AIVAQMVSEGISEAQARKQVFMVDRWGMLQSNMPNLLPFQQKLAQNCDDITSWDNFSDNISLLDVVNNAKPTVLIGVSGAPGLFTEEIIKAM 401