DY260014

Overview
NameDY260014
Unique NameDY260014
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1066
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DY260014_ssr1 microsatellite DY260014_ssr1:1..14. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig14882 contig Ccv1_Contig14882:1..1066. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA722_ARATH (Vesicle-associated membrane protein 722 OS=Arabidopsis thaliana GN=VAMP722 PE=2 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 1.305e-63
Identity = 63/72 (87.50%), Postives = 69/72 (95.83%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            M QQSLIYSFVARGTVIL E+T+F GNFTSIA+QCLQKLP++NNKFTYNCDGHTFNYLVENGFTYCVVAV+S
Sbjct:    1 MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTYCVVAVDS 72

HSP 2 Score: 108.997 bits (271), Expect = 1.305e-63
Identity = 51/61 (83.61%), Postives = 58/61 (95.08%), Query Frame = 1 
Query:  307 LNPAGRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPPEEITK 489
            ++ AGRQIP+AFLERVKEDF+KRYGGGKAATA ANSLNKEFGSKLKEHMQYC+D P+EI+K
Sbjct:   70 VDSAGRQIPMAFLERVKEDFNKRYGGGKAATAQANSLNKEFGSKLKEHMQYCMDHPDEISK 130

HSP 3 Score: 40.0466 bits (92), Expect = 1.305e-63
Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 2 
Query:  491 LAKVQAQVSEVRGVMMEKIEKVP*SG*EKSSLPVHQIE 604
            LAKV+AQVSEV+GVMME IEKV   G EK  L V + E
Sbjct:  131 LAKVKAQVSEVKGVMMENIEKVLDRG-EKIELLVDKTE 167
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA725_ARATH (Vesicle-associated membrane protein 725 OS=Arabidopsis thaliana GN=VAMP725 PE=2 SV=2)

HSP 1 Score: 141.739 bits (356), Expect = 1.689e-63
Identity = 67/78 (85.90%), Postives = 74/78 (94.87%), Query Frame = 3 
Query:   84 VRVLR-MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            +RV R MGQQ+LIYSFVARGTVIL EYTEF GNFT++A+QCLQKLP++NNKFTYNCDGHTFNYLVENGFTYCVVAVES
Sbjct:   60 LRVTRTMGQQNLIYSFVARGTVILVEYTEFKGNFTAVAAQCLQKLPSSNNKFTYNCDGHTFNYLVENGFTYCVVAVES 137

HSP 2 Score: 105.531 bits (262), Expect = 1.689e-63
Identity = 49/57 (85.96%), Postives = 54/57 (94.74%), Query Frame = 1 
Query:  319 GRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPPEEITK 489
            GRQIP+AFLERVKEDF+KRYGGGKA TA ANSLN+EFGSKLKEHMQYCVD P+EI+K
Sbjct:  139 GRQIPMAFLERVKEDFNKRYGGGKATTAQANSLNREFGSKLKEHMQYCVDHPDEISK 195

HSP 3 Score: 38.891 bits (89), Expect = 1.689e-63
Identity = 24/38 (63.16%), Postives = 28/38 (73.68%), Query Frame = 2 
Query:  491 LAKVQAQVSEVRGVMMEKIEKVP*SG*EKSSLPVHQIE 604
            LAKV+AQV+EV+GVMME IEKV   G EK  L V + E
Sbjct:  196 LAKVKAQVTEVKGVMMENIEKVLDRG-EKIELLVDKTE 232
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA721_ARATH (Vesicle-associated membrane protein 721 OS=Arabidopsis thaliana GN=VAMP721 PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.078e-62
Identity = 62/72 (86.11%), Postives = 69/72 (95.83%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            M QQSLIYSFVARGTVIL E+T+F GNFTSIA+QCLQKLP++NNKFTYNCDGHTFNYLVE+GFTYCVVAV+S
Sbjct:    1 MAQQSLIYSFVARGTVILVEFTDFKGNFTSIAAQCLQKLPSSNNKFTYNCDGHTFNYLVEDGFTYCVVAVDS 72

HSP 2 Score: 107.842 bits (268), Expect = 1.078e-62
Identity = 50/61 (81.97%), Postives = 58/61 (95.08%), Query Frame = 1 
Query:  307 LNPAGRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPPEEITK 489
            ++ AGRQIP++FLERVKEDF+KRYGGGKAATA ANSLNKEFGSKLKEHMQYC+D P+EI+K
Sbjct:   70 VDSAGRQIPMSFLERVKEDFNKRYGGGKAATAQANSLNKEFGSKLKEHMQYCMDHPDEISK 130

HSP 3 Score: 40.0466 bits (92), Expect = 1.078e-62
Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 2 
Query:  491 LAKVQAQVSEVRGVMMEKIEKVP*SG*EKSSLPVHQIE 604
            LAKV+AQVSEV+GVMME IEKV   G EK  L V + E
Sbjct:  131 LAKVKAQVSEVKGVMMENIEKVLDRG-EKIELLVDKTE 167
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA723_ARATH (Vesicle-associated membrane protein 723 OS=Arabidopsis thaliana GN=VAMP723 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 6.603e-55
Identity = 56/72 (77.78%), Postives = 65/72 (90.28%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            M QQSL YSF+ARGTVIL E+T+F GNFTS+A+Q L+ LP++NNKFTYNCDGHTFN LVENGFTYCVVAV+S
Sbjct:    1 MAQQSLFYSFIARGTVILVEFTDFKGNFTSVAAQYLENLPSSNNKFTYNCDGHTFNDLVENGFTYCVVAVDS 72

HSP 2 Score: 98.9821 bits (245), Expect = 6.603e-55
Identity = 46/60 (76.67%), Postives = 54/60 (90.00%), Query Frame = 1 
Query:  307 LNPAGRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPPEEIT 486
            ++ AGR+IP+AFLERVKEDF+KRYGG KAAT  ANSLNKEFGS LKEHMQYC+D P+EI+
Sbjct:   70 VDSAGREIPMAFLERVKEDFYKRYGGEKAATDQANSLNKEFGSNLKEHMQYCMDHPDEIS 129

HSP 3 Score: 34.2686 bits (77), Expect = 6.603e-55
Identity = 16/23 (69.57%), Postives = 20/23 (86.96%), Query Frame = 2 
Query:  488 SLAKVQAQVSEVRGVMMEKIEKV 556
            +LAK +AQVSEV+ +MME IEKV
Sbjct:  130 NLAKAKAQVSEVKSLMMENIEKV 152
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA724_ARATH (Vesicle-associated membrane protein 724 OS=Arabidopsis thaliana GN=VAMP724 PE=2 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 9.876e-49
Identity = 57/73 (78.08%), Postives = 67/73 (91.78%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLP-ATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            MGQ+S IYSFVARGT+ILAEYTEFTGNF SIA+QCLQKLP ++N+KFTYNCD HTFN+LVE+G+ YCVVA +S
Sbjct:    1 MGQESFIYSFVARGTMILAEYTEFTGNFPSIAAQCLQKLPSSSNSKFTYNCDHHTFNFLVEDGYAYCVVAKDS 73

HSP 2 Score: 84.3445 bits (207), Expect = 9.876e-49
Identity = 41/56 (73.21%), Postives = 45/56 (80.36%), Query Frame = 1 
Query:  322 RQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPPEEITK 489
            +QI IAFLERVK DF KRYGGGKA+TA+A SLNKEFG  +KEHM Y VD  EEI K
Sbjct:   76 KQISIAFLERVKADFKKRYGGGKASTAIAKSLNKEFGPVMKEHMNYIVDHAEEIEK 131

HSP 3 Score: 30.4166 bits (67), Expect = 9.876e-49
Identity = 13/21 (61.90%), Postives = 18/21 (85.71%), Query Frame = 2 
Query:  491 LAKVQAQVSEVRGVMMEKIEK 553
            L KV+AQVSEV+ +M+E I+K
Sbjct:  132 LIKVKAQVSEVKSIMLENIDK 152
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA727_ARATH (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana GN=VAMP727 PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 4.934e-39
Identity = 48/72 (66.67%), Postives = 63/72 (87.50%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            M Q+ LIYSFVA+GTV+LAE+T ++GNF++IA QCLQKLP  ++K+TY+CDGHTFN+LV+NGF + VVA ES
Sbjct:    1 MSQKGLIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVDNGFVFLVVADES 72

HSP 2 Score: 59.6918 bits (143), Expect = 4.934e-39
Identity = 33/76 (43.42%), Postives = 41/76 (53.95%), Query Frame = 1 
Query:  319 GRQIPIAFLERVKEDFHKRYGGG-------------------KAATAVANSLNKEFGSKLKEHMQYCVDPPEEITK 489
            GR +P  FLERVKEDF KRY                          +VA +L++EFG  LKEHMQYC+  PEE++K
Sbjct:   74 GRSVPFVFLERVKEDFKKRYEASIKNDERHPLADEDEDDDLFGDRFSVAYNLDREFGPILKEHMQYCMSHPEEMSK 149

HSP 3 Score: 34.6538 bits (78), Expect = 4.934e-39
Identity = 19/38 (50.00%), Postives = 28/38 (73.68%), Query Frame = 2 
Query:  491 LAKVQAQVSEVRGVMMEKIEKVP*SG*EKSSLPVHQIE 604
            L+K++AQ++EV+G+MM+ IEKV   G EK  L V + E
Sbjct:  150 LSKLKAQITEVKGIMMDNIEKVLDRG-EKIELLVDKTE 186
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA726_ARATH (Putative vesicle-associated membrane protein 726 OS=Arabidopsis thaliana GN=VAMP726 PE=2 SV=2)

HSP 1 Score: 140.584 bits (353), Expect = 1.512e-32
Identity = 86/195 (44.10%), Postives = 103/195 (52.82%), Query Frame = 3 
Query:   99 MGQQSLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVESCWQANSNCLSGAS*GGFPQEIWWRKSCNGCCK*PEQRVWV*TKGAHAVLCGSS*RDHQALPKSKPRFPKSGGL*WKKLRKFLDLGEKNRASRFTKSNTLPSPGHKISRHPGDQKTKRNTWIPN 683
            MGQQSLIYSFVARGTVILAEYTEF GNFTS+A+QCLQKLP++NNKFTYNCDGHTFNYL +NGFTYCVV +ES  +             F +     K+         +      K  H   C     +   L K K +  +  G+  + + K LD GEK       K+  L S            K KR  W  N
Sbjct:    1 MGQQSLIYSFVARGTVILAEYTEFKGNFTSVAAQCLQKLPSSNNKFTYNCDGHTFNYLADNGFTYCVVVIESAGRQIPMAFLERVKEDFNKRYGGGKASTAKANSLNKEFGSKLK-EHMQYCADHPEEISKLSKVKAQVTEVKGVMMENIEKVLDRGEKIEL-LVDKTENLRSQAQDFRTQ--GTKMKRKLWFEN 191
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VAM7B_DICDI (Vesicle-associated membrane protein 7B OS=Dictyostelium discoideum GN=vamp7B PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 9.317e-13
Identity = 27/66 (40.91%), Postives = 41/66 (62.12%), Query Frame = 3 
Query:  114 LIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATNNKFTYNCDGHTFNYLVENGFTYCVVAVE 311
            +IYS VARG+ +LAE+T   GNF +I  + L  +P  + K +Y  + + F+YLV +  TY  +A E
Sbjct:    3 IIYSLVARGSSVLAEFTSTNGNFVTITRRILDLIPPNDTKMSYVYEKYIFHYLVSDTLTYLCMADE 68

HSP 2 Score: 37.3502 bits (85), Expect = 9.317e-13
Identity = 19/47 (40.43%), Postives = 26/47 (55.32%), Query Frame = 1 
Query:  319 GRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQY 459
            GR+IP  FL+ VK  F   Y G K  TA+A  +N +F   L+  M +
Sbjct:   71 GRRIPFTFLDDVKNRFKSMY-GDKGKTAIAYGMNSDFSRTLENLMDH 116
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA714_ARATH (Vesicle-associated membrane protein 714 OS=Arabidopsis thaliana GN=VAMP714 PE=2 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 1.094e-11
Identity = 22/67 (32.84%), Postives = 43/67 (64.18%), Query Frame = 3 
Query:  111 SLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKL--PATNNKFTYNCDGHTFNYLVENGFTYCVVA 305
            +++Y+ VARGTV+LAE++  TGN  ++  + L+KL    ++ +  ++ D + F+ L  +G T+  +A
Sbjct:    2 AIVYAVVARGTVVLAEFSAVTGNTGAVVRRILEKLSPEISDERLCFSQDRYIFHILRSDGLTFLCMA 68

HSP 2 Score: 34.2686 bits (77), Expect = 1.094e-11
Identity = 18/52 (34.62%), Postives = 28/52 (53.85%), Query Frame = 1 
Query:  319 GRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPP 474
            GR++P ++LE +   F K Y G  A  A A ++N EF   L + M++    P
Sbjct:   73 GRRVPFSYLEEIHMRFMKNY-GKVAHNAPAYAMNDEFSRVLHQQMEFFSSNP 123

HSP 3 Score: 29.6462 bits (65), Expect = 1.094e-11
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = 2 
Query:  488 SLAKVQAQVSEVRGVMMEKIEKV 556
            +L +V+ +VSE+R VM+E IEK+
Sbjct:  127 TLNRVRGEVSEIRSVMVENIEKI 149
[Close]
BLAST of DY260014 vs. ExPASy Swiss-Prot
Match: VA711_ARATH (Vesicle-associated membrane protein 711 OS=Arabidopsis thaliana GN=VAMP711 PE=2 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 1.280e-11
Identity = 25/69 (36.23%), Postives = 45/69 (65.22%), Query Frame = 3 
Query:  111 SLIYSFVARGTVILAEYTEFTGNFTSIASQCLQKLPATN-NKFTYNCDGHTFNYLVENGFTYCVVAVES 314
            +++Y+ VARGTV+L+E+T  + N ++IA Q L+K+P  N +  +Y+ D + F+    +G T   +A E+
Sbjct:    2 AILYALVARGTVVLSEFTATSTNASTIAKQILEKVPGDNDSNVSYSQDRYVFHVKRTDGLTVLCMAEET 70

HSP 2 Score: 40.817 bits (94), Expect = 1.280e-11
Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  316 AGRQIPIAFLERVKEDFHKRYGGGKAATAVANSLNKEFGSKLKEHMQYCVDPP 474
            AGR+IP AFLE + + F + YG     TA+A ++N+EF   L + + Y  + P
Sbjct:   71 AGRRIPFAFLEDIHQRFVRTYGRA-VHTALAYAMNEEFSRVLSQQIDYYSNDP 122
The following BLAST results are available for this feature:
BLAST of DY260014 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10
Match NameE-valueIdentityDescription
VA722_ARATH1.305e-6387.50Vesicle-associated membrane protein 722 OS=Arabido... [more]
VA725_ARATH1.689e-6385.90Vesicle-associated membrane protein 725 OS=Arabido... [more]
VA721_ARATH1.078e-6286.11Vesicle-associated membrane protein 721 OS=Arabido... [more]
VA723_ARATH6.603e-5577.78Vesicle-associated membrane protein 723 OS=Arabido... [more]
VA724_ARATH9.876e-4978.08Vesicle-associated membrane protein 724 OS=Arabido... [more]
VA727_ARATH4.934e-3966.67Vesicle-associated membrane protein 727 OS=Arabido... [more]
VA726_ARATH1.512e-3244.10Putative vesicle-associated membrane protein 726 O... [more]
VAM7B_DICDI9.317e-1340.91Vesicle-associated membrane protein 7B OS=Dictyost... [more]
VA714_ARATH1.094e-1132.84Vesicle-associated membrane protein 714 OS=Arabido... [more]
VA711_ARATH1.280e-1136.23Vesicle-associated membrane protein 711 OS=Arabido... [more]
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Properties
Property NameValue
Genbank descriptionKN0AAP10YP04FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260014 ID=DY260014; Name=DY260014; organism=Citrus clementina; type=EST; length=1066bp
CTCTCTCTCTCTCTAATTTTTGTTTTTTCTGCTCCTCATTTTTTTTTGTC
AATTGAATCTTTCATCTCGAATTTGAATCTAGGGTTAGGGTTTTGAGGAT
GGGCCAACAGTCGTTGATCTATAGCTTCGTGGCTCGAGGCACGGTGATCC
TCGCCGAGTACACTGAGTTCACCGGTAATTTCACGAGCATCGCATCGCAA
TGTCTCCAGAAACTTCCCGCTACCAATAACAAGTTCACCTACAACTGCGA
TGGCCACACCTTCAATTACCTCGTTGAGAACGGCTTCACCTATTGTGTGG
TTGCTGTTGAATCCTGCTGGCAGGCAAATTCCAATTGCCTTTCTGGAGCG
AGTTAAGGAGGATTTCCACAAGAGATATGGTGGAGGAAAAGCTGCAACGG
CTGTTGCAAATAGCCTGAACAAAGAGTTTGGGTCTAAACTAAAGGAGCAC
ATGCAGTATTGTGTGGATCCTCCTGAAGAGATCACCAAGCCTTGCCAAAG
TCCAAGCCCAGGTTTCCGAAGTCAGGGGGGTTATGATGGAAAAAATTGAG
AAAGTTCCTTGATCTGGGTGAGAAAAATCGAGCCTCCCGGTTCACCAAAT
CGAATACCCTTCCGTCCCCGGGCCACAAAATTTCCCGACACCCGGGGGAC
CAAAAAACCAAACGAAACACGTGGATCCCCAACAAAGCAAAATAAACTCG
ACGCCTTTTCGGGTAACCCCCTCACCTTTACCCCTTACCTCCCTCTCGCA
ATCTTCCCCAGCCCTCCCTCACTCGTCTGCCTCCTCTACACACCCCCGGA
CCACCGGCACAGATCCTCCCACACCCTGCACCATACTCATTCACAAAGCC
CAACACCTAAAGAACCGCCCACTCCCTCCCATCATTTACCGTCTCACACC
AGCGCCACCCCCTACTGACCACCCCCCCACCACAACACCCATATCTTCTT
TACCCGCCTAAAACACAGTCTCTACCCCCTTCCTTCCCCAATCTCCTCTA
CGCCCCCCCACCCCCTCATACCCGCCTTCTCTTCTCCTCTCTCTTTCTCC
TCACTTTCTCTCCTTT
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