DY260116

Overview
NameDY260116
Unique NameDY260116
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length976
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_CITPA (Inositol-3-phosphate synthase OS=Citrus paradisi PE=3 SV=1)

HSP 1 Score: 352.829 bits (904), Expect = 1.715e-96
Identity = 188/253 (74.31%), Postives = 206/253 (81.42%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPI---------KGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIENFKVESPNVKYTD+EIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGI WATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADA+ARARVFDIDL  QLR       P++ESM  + LP I         +G       K  +KEQ LQII++I R  ++K + +RV   +  AN E+   +++G
Sbjct:    1 MFIENFKVESPNVKYTDHEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGICWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADAMARARVFDIDLQKQLR-------PYMESM--VPLPGIYDPDFIAANQGSRANNVIKGTKKEQMLQIIKDI-REFKEKNKVDRV-VVLWTANTERYSNVIVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_NICPA (Inositol-3-phosphate synthase OS=Nicotiana paniculata GN=INPS1 PE=2 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 8.246e-91
Identity = 176/253 (69.57%), Postives = 202/253 (79.84%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPI---------KGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIENFKVESPNVKYT++EIHSVYDY+TTELVH+ +NGTYQW VKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKD VQQANYFGSLTQAS IRVGS+NGEEIYAPFKS+LPMVNPDD+VFGGWDISDMNLADA+ARA+VFDIDL  QLR       P++ESM  + LP I         +G       K  +KEQ  QII++I R  ++K + ++V   +  AN E+   +V+G
Sbjct:    1 MFIENFKVESPNVKYTESEIHSVYDYQTTELVHDEKNGTYQWTVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKVQQANYFGSLTQASTIRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISDMNLADAMARAKVFDIDLQKQLR-------PYMESM--VPLPGIYDPDFIAANQGSRANNVIKGTKKEQIDQIIKDI-REFKEKNKVDKV-VVLWTANTERYSNVVVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_TOBAC (Inositol-3-phosphate synthase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 2.399e-90
Identity = 176/253 (69.57%), Postives = 201/253 (79.45%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPI---------KGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIENFKVESPNVKYT++EIHSVYDY+TTELVHE +NGTYQW VKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKD VQQANYFGSLTQAS IRVGS+NGEEIYAPFKS+LPMVNPDD+VFGGWDIS MNLADA+ARA+VFDIDL  QLR       P++ESM  + LP I         +G       K  +KEQ  QII++I R  ++K + ++V   +  AN E+   +V+G
Sbjct:    1 MFIENFKVESPNVKYTESEIHSVYDYQTTELVHEEKNGTYQWTVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKVQQANYFGSLTQASTIRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISGMNLADAMARAKVFDIDLQKQLR-------PYMESM--VPLPGIYDPDFIAANQGSRANNVIKGTKKEQIDQIIKDI-REFKEKNKVDKV-VVLWTANTERYSNVVVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_WHEAT (Inositol-3-phosphate synthase OS=Triticum aestivum GN=MIPS PE=2 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 3.464e-89
Identity = 168/244 (68.85%), Postives = 195/244 (79.92%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEIHSVYDYETTE+VHENRNGTYQW+VKPKTVKY+FKTD  VPKLGVMLVGWGGNNGSTLT GVIAN+EGISWATKD VQQANYFGSLTQAS+IRVGSYNGEEIYAPFKS+LPMVNPDD+VFGGWDISDMNLADA+ARARV DIDL  QLRP      P         +   +G    +  K  +KEQ  QII+++ R  ++K + +++   +  AN E+   +V+G
Sbjct:    1 MFIESFKVESPNVKYTENEIHSVYDYETTEVVHENRNGTYQWVVKPKTVKYDFKTDTRVPKLGVMLVGWGGNNGSTLTAGVIANKEGISWATKDKVQQANYFGSLTQASSIRVGSYNGEEIYAPFKSLLPMVNPDDVVFGGWDISDMNLADAMARARVLDIDLQKQLRPYMEHMVPLPGIYDPDFIAANQGSRANSVIKGTKKEQVDQIIKDM-REFKEKNKVDKL-VVLWTANTERYSDVVVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_SESIN (Inositol-3-phosphate synthase OS=Sesamum indicum PE=2 SV=1)

HSP 1 Score: 325.479 bits (833), Expect = 2.933e-88
Identity = 167/244 (68.44%), Postives = 194/244 (79.51%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+ EIHSVY+YETTELVHE+RNGTYQWIVKPKTVKYEFKTD HVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKD VQQANYFGSLTQAS+IRVGS+NGEEIYAPFKS+LPMVNPDD+VFGGWDIS+MNLADA+ RA+V DIDL  QLRP      P         +   +G       K  +KEQ  QII+++ R  +++ + ++V   +  AN E+   +V+G
Sbjct:    1 MFIESFKVESPNVKYTEGEIHSVYNYETTELVHESRNGTYQWIVKPKTVKYEFKTDTHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKVQQANYFGSLTQASSIRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISNMNLADAMGRAKVLDIDLQKQLRPYMEHMVPLPGIYDPDFIAANQGSRANNVIKGTKKEQVQQIIKDM-RDFKEQNKVDKV-VVLWTANTERYSNVVVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO2_ARATH (Inositol-3-phosphate synthase isozyme 2 OS=Arabidopsis thaliana GN=At2g22240 PE=2 SV=2)

HSP 1 Score: 322.398 bits (825), Expect = 2.483e-87
Identity = 164/244 (67.21%), Postives = 194/244 (79.51%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEI+SVYDYETTE+VHENRNGTYQW+VKPKTVKY+FKTD  VPKLGVMLVGWGGNNGSTLT GVIAN+EGISWATKD VQQANYFGSLTQAS+IRVGSYNGEEIYAPFKS+LPMVNP+D+VFGGWDISDMNLADA+ARARV DIDL  QLRP      P         +   +G    +  K  +KEQ   II+++ R  ++K + +++   +  AN E+   +++G
Sbjct:    1 MFIESFKVESPNVKYTENEINSVYDYETTEVVHENRNGTYQWVVKPKTVKYDFKTDTRVPKLGVMLVGWGGNNGSTLTAGVIANKEGISWATKDKVQQANYFGSLTQASSIRVGSYNGEEIYAPFKSLLPMVNPEDVVFGGWDISDMNLADAMARARVLDIDLQKQLRPYMENMIPLPGIYDPDFIAANQGSRANSVIKGTKKEQVDHIIKDM-REFKEKNKVDKL-VVLWTANTERYSNVIVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO3_ARATH (Probable inositol-3-phosphate synthase isozyme 3 OS=Arabidopsis thaliana GN=At5g10170 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 7.224e-87
Identity = 163/244 (66.80%), Postives = 193/244 (79.10%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEIHSVYDY+TTELVHEN+NG +QW VKPKTVKYEFKTD HVPKLGVMLVGWGGNNGSTLT GVIANREGISWATK+ VQQANYFGSLTQAS+IRVGS+NGEEIYAPFKS+LPMVNP++IVFGGWDISDMNLADA+ARA+V DIDL  Q+RP      P         +   +G       K  +K+Q  Q+I++I R  ++K + ++V   +  AN E+   +V+G
Sbjct:    1 MFIESFKVESPNVKYTENEIHSVYDYQTTELVHENKNGAFQWTVKPKTVKYEFKTDTHVPKLGVMLVGWGGNNGSTLTAGVIANREGISWATKEKVQQANYFGSLTQASSIRVGSFNGEEIYAPFKSLLPMVNPEEIVFGGWDISDMNLADAMARAKVLDIDLQKQMRPFMEHMVPLPGIFDPDFIAANQGSRANHVIKGTKKQQLEQVIKDI-REFKEKNKVDKV-VVLWTANTERYSNVVVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_BRANA (Inositol-3-phosphate synthase OS=Brassica napus PE=2 SV=2)

HSP 1 Score: 315.464 bits (807), Expect = 3.035e-85
Identity = 161/244 (65.98%), Postives = 190/244 (77.87%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRNGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEIHSVYDYETTE+VHEN NG YQWIVKPK VKY+FKTD  VPKLGVMLVGWGGNNGSTLT GVIAN+EGISWATKD VQQANYFGSLTQAS+IRVGS+NGEE+YAPFKS++PMVNPDD+VFGGWDISDMNLADA+ RA+V DIDL  QLRP      P         +   +G       K  +KEQ  QII+++ R  ++K + ++V   +  AN E+   +++G
Sbjct:    1 MFIESFKVESPNVKYTENEIHSVYDYETTEVVHENVNGAYQWIVKPKVVKYDFKTDTRVPKLGVMLVGWGGNNGSTLTAGVIANKEGISWATKDKVQQANYFGSLTQASSIRVGSFNGEEMYAPFKSLVPMVNPDDVVFGGWDISDMNLADAMGRAKVLDIDLQKQLRPYMENIVPLPGIYDPDFIAANQGSRANNVIKGTKKEQVDQIIKDM-REFKEKNKVDKV-VVLWTANTERYSNVIVG 242          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_MESCR (Inositol-3-phosphate synthase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 313.153 bits (801), Expect = 1.506e-84
Identity = 163/246 (66.26%), Postives = 190/246 (77.24%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENRN--GTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEI SVY+Y+TTELVHENR   G YQWIVKPKTV+Y FKTD  VPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKD +QQANYFGSLTQAS+IRVGS+NGEEIYAPFKS+LPMVNPDD+VFGGWDISDMNLADA+ RARVFDIDL  QLRP      P         +   +G       K  +KEQ  ++I++I R  ++K + ++V   +   N E+   +V+G
Sbjct:    1 MFIESFKVESPNVKYTENEIESVYNYDTTELVHENRKDAGGYQWIVKPKTVQYHFKTDTRVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDKIQQANYFGSLTQASSIRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISDMNLADAMTRARVFDIDLQKQLRPYMEHMVPLPGIYDPDFIAANQGSRANNVIKGTKKEQVERVIKDI-REFKEKNKVDKV-VVLWTGNTERYSNVVVG 244          
BLAST of DY260116 vs. ExPASy Swiss-Prot
Match: INO1_ARATH (Inositol-3-phosphate synthase isozyme 1 OS=Arabidopsis thaliana GN=At4g39800 PE=2 SV=3)

HSP 1 Score: 312.383 bits (799), Expect = 2.569e-84
Identity = 165/245 (67.35%), Postives = 191/245 (77.96%), Query Frame = 2
Query:  245 MFIENFKVESPNVKYTDNEIHSVYDYETTELVHENR-NGTYQWIVKPKTVKYEFKTDVHVPKLGVMLVGWGGNNGSTLTGGVIANREGISWATKDTVQQANYFGSLTQASAIRVGSYNGEEIYAPFKSILPMVNPDDIVFGGWDISDMNLADALARARVFDIDLPSQLRPTWNQWSPFLESMTLISLPPIKGPVPTT*SKEPRKEQCLQIIQNI*RV*RKKTRWNRVGCPVGPANPEKVPLMVIG 976
            MFIE+FKVESPNVKYT+NEIHSVYDYETTE+VHE   NGTYQWIVKPKTVKY+FKTD+ VPKLGVMLVG GGNNGSTLT GVIAN+EGISWATKD VQQANYFGSLTQAS+IRVGS+NGEEIYAPFKS+LPMVNPDD+VFGGWDISDMNLADA+ARARV DIDL  QLRP      P         +   +G       K  +KEQ   II+++ R  ++K + ++V   +  AN E+   +V+G
Sbjct:    1 MFIESFKVESPNVKYTENEIHSVYDYETTEVVHEKTVNGTYQWIVKPKTVKYDFKTDIRVPKLGVMLVGLGGNNGSTLTAGVIANKEGISWATKDKVQQANYFGSLTQASSIRVGSFNGEEIYAPFKSLLPMVNPDDVVFGGWDISDMNLADAMARARVLDIDLQKQLRPYMENIVPLPGIFDPDFIAANQGSRANHVIKGTKKEQVDHIIKDM-REFKEKNKVDKV-VVLWTANTERYSNVVVG 243          
The following BLAST results are available for this feature:
BLAST of DY260116 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
INO1_CITPA1.715e-9674.31Inositol-3-phosphate synthase OS=Citrus paradisi P... [more]
INO1_NICPA8.246e-9169.57Inositol-3-phosphate synthase OS=Nicotiana panicul... [more]
INO1_TOBAC2.399e-9069.57Inositol-3-phosphate synthase OS=Nicotiana tabacum... [more]
INO1_WHEAT3.464e-8968.85Inositol-3-phosphate synthase OS=Triticum aestivum... [more]
INO1_SESIN2.933e-8868.44Inositol-3-phosphate synthase OS=Sesamum indicum P... [more]
INO2_ARATH2.483e-8767.21Inositol-3-phosphate synthase isozyme 2 OS=Arabido... [more]
INO3_ARATH7.224e-8766.80Probable inositol-3-phosphate synthase isozyme 3 O... [more]
INO1_BRANA3.035e-8565.98Inositol-3-phosphate synthase OS=Brassica napus PE... [more]
INO1_MESCR1.506e-8466.26Inositol-3-phosphate synthase OS=Mesembryanthemum ... [more]
INO1_ARATH2.569e-8467.35Inositol-3-phosphate synthase isozyme 1 OS=Arabido... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP11YF21FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260116 ID=DY260116; Name=DY260116; organism=Citrus clementina; type=EST; length=976bp
CGAAAGTTAAAATAGTGTTGAGTCCAGTGGGAAGTGAGAATTGAGAAGGA
GAGTCCAGTTCGGAGACAGCAAGACGAAACAGCTCTTCTTCCAATAAATC
TCAAAGCATTTGTTTGGTTTTATTTAAAGCAATTTTCTCAGCAAACTCTT
CTTCTAATAAATTTTAAAGCATTTGTTTGGTTTTTATTTAAAGCAATTTT
CTCAGCGAAGTTTTCAATTTTTTCTCTAAAATTTTCCTGGGAAAATGTTT
ATCGAAAATTTCAAGGTTGAGAGCCCAAATGTGAAGTACACAGATAATGA
GATTCACTCTGTGTACGATTATGAAACCACTGAGCTTGTTCATGAGAACA
GAAATGGCACTTATCAGTGGATTGTCAAGCCCAAAACTGTCAAATATGAG
TTCAAAACCGATGTTCATGTCCCTAAACTTGGGGTTATGCTTGTGGGTTG
GGGTGGAAACAACGGCTCAACTCTCACGGGTGGTGTTATCGCGAATCGAG
AGGGAATCTCCTGGGCCACAAAGGACACAGTGCAACAAGCCAATTATTTT
GGTTCACTTACCCAAGCATCAGCAATTAGAGTTGGGTCTTACAATGGAGA
GGAGATTTATGCTCCATTCAAGAGCATTCTCCCTATGGTGAACCCAGATG
ACATTGTGTTTGGAGGATGGGACATTAGTGACATGAACTTGGCTGATGCC
CTGGCCAGGGCTAGAGTGTTTGATATTGATCTTCCAAGCCAACTACGGCC
TACATGGAATCAATGGTCCCCCTTCCTGGAATCTATGACCTTGATTTCAT
TGCCGCCAATCAAGGGTCCCGTGCCCACAACGTGATCCAAGGAACCCAGG
AAAGAACAATGCCTACAAATCATCCAAAATATTTAAAGAGTTTAAAGGAA
AAAAACCAGGTGGAATAGGGTTGGTTGTCCCGTGGGACCGGCCAACCCCG
AAAAGGTACCGTTAATGGTGATTGGT
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