FC869021

Overview
NameFC869021
Unique NameFC869021
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length738
Libraries
Library NameType
AbioticL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: GOX_SPIOL (Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 337.806 bits (865), Expect = 3.670e-92
Identity = 171/203 (84.24%), Postives = 182/203 (89.66%), Query Frame = 1
Query:  130 EITNVMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGPGIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGLYIGKMDKTDDSGLASYVA 738
            EITNV EYEA+AK+KLPKMVYDYYASGAEDQWTL ENRNAFSRILFRPRIL DV+ IDMTTT+LGF ISMPIMIAPTA QKMAHPEGE          GTIMTLSSWATSSVEEV+STGPGIRFFQLYV KDRNV AQLV+RAERAGFKAIALTVDTPRLGRREADIKNRFVLPP LTLKN+EG+ +GKMDK +DSGL+SYVA
Sbjct:    2 EITNVNEYEAIAKQKLPKMVYDYYASGAEDQWTLAENRNAFSRILFRPRILIDVTNIDMTTTILGFKISMPIMIAPTAMQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLKNFEGIDLGKMDKANDSGLSSYVA 204          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: GOX2_ARATH (Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 OS=Arabidopsis thaliana GN=At3g14420 PE=1 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 3.107e-91
Identity = 168/203 (82.76%), Postives = 183/203 (90.15%), Query Frame = 1
Query:  130 EITNVMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGPGIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGLYIGKMDKTDDSGLASYVA 738
            EITNV EY+A+AK+KLPKMVYDYYASGAEDQWTLQENRNAF+RILFRPRIL DVSKIDMTTTVLGF ISMPIM+APTA QKMAHP+GE          GTIMTLSSWATSSVEEV+STGPGIRFFQLYV K+RNV  QLV+RAERAGFKAIALTVDTPRLGRRE+DIKNRF LPP+LTLKN+EGL +GKMD+ +DSGLASYVA
Sbjct:    2 EITNVTEYDAIAKQKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVSKIDMTTTVLGFKISMPIMVAPTAMQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKNRNVVEQLVRRAERAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVA 204          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: GOX1_ARATH (Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 OS=Arabidopsis thaliana GN=At3g14415 PE=1 SV=1)

HSP 1 Score: 329.331 bits (843), Expect = 1.305e-89
Identity = 165/203 (81.28%), Postives = 181/203 (89.16%), Query Frame = 1
Query:  130 EITNVMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGPGIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGLYIGKMDKTDDSGLASYVA 738
            EITNV EY+A+AK KLPKMVYDYYASGAEDQWTLQENRNAF+RILFRPRIL DV+KIDM TTVLGF ISMPIM+APTAFQKMAHP+GE          GTIMTLSSWATSSVEEV+STGPGIRFFQLYV K+R V  QLV+RAE+AGFKAIALTVDTPRLGRRE+DIKNRF LPP+LTLKN+EGL +GKMD+ +DSGLASYVA
Sbjct:    2 EITNVTEYDAIAKAKLPKMVYDYYASGAEDQWTLQENRNAFARILFRPRILIDVNKIDMATTVLGFKISMPIMVAPTAFQKMAHPDGEYATARAASAAGTIMTLSSWATSSVEEVASTGPGIRFFQLYVYKNRKVVEQLVRRAEKAGFKAIALTVDTPRLGRRESDIKNRFTLPPNLTLKNFEGLDLGKMDEANDSGLASYVA 204          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX1_HUMAN (Hydroxyacid oxidase 1 OS=Homo sapiens GN=HAO1 PE=1 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.321e-49
Identity = 104/203 (51.23%), Postives = 139/203 (68.47%), Query Frame = 1
Query:  142 VMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGP-GIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGLYIG---KMDKTDDSGLASYVA 738
            + +YE  AK  LPK +YDYY SGA D+ TL +N  AFSR    PR+LR+V++ D++T+VLG  +SMPI +  TA Q+MAH +GE          GT M LSSWATSS+EEV+  GP  +R+ QLY+ KDR V  +LV++AE+ G+KAI +TVDTP LG R  D++NRF LPP L +KN+E   +    + +  DDSGLA+YVA
Sbjct:    8 INDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVA 210          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX1_MOUSE (Hydroxyacid oxidase 1 OS=Mus musculus GN=Hao1 PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 3.842e-49
Identity = 105/203 (51.72%), Postives = 135/203 (66.50%), Query Frame = 1
Query:  142 VMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGP-GIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEG---LYIGKMDKTDDSGLASYVA 738
            + +YE   +  L K VYDYY SGA DQ TL +N  AFSR    PR+LR+V+ ID++T+VLG  +SMPI +  TA Q MAH +GE          GT M LSSWATSS+EEV+  GP  +R+ QLY+ KDR +  Q+VKRAE+ G+KAI +TVDTP LG R  D++NRF LPP L +KN+E     +  K +  D+SGLA YVA
Sbjct:    8 ISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVA 210          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX_DICDI (Hydroxyacid oxidase OS=Dictyostelium discoideum GN=haox PE=3 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 5.193e-46
Identity = 109/217 (50.23%), Postives = 136/217 (62.67%), Query Frame = 1
Query:   91 SRKPSDSIMTYISEITNVMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSS-TGPGIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGLYIGKMDKTDDSGLASYVA 738
            S K ++ + +++S    V E    AK  LPKM YDYYASG+ DQ TL EN NAFSRI   PR L DVSK++  T + G +IS PI+IAP A Q+MA   GE           TIMTLSS +T+SVE++SS T     +FQLYV KDR V  +LVKRAE  G+ A+ LTVDTP LG+R AD KN F LP  L+LK +E L +  +    D GL  Y+A
Sbjct:   21 SNKKTNRLDSFVS----VSELHEEAKRLLPKMAYDYYASGSNDQITLAENENAFSRIKLVPRSLVDVSKVNTKTRIFGRDISTPILIAPWAMQRMASQRGELDTVEASKEFNTIMTLSSLSTTSVEDLSSATNGNPGWFQLYVFKDRKVSEELVKRAESIGYSALVLTVDTPFLGKRTADFKNSFKLPNGLSLKIFEKLMLSNL----DGGLNQYIA 229          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX2_BOVIN (Hydroxyacid oxidase 2 OS=Bos taurus GN=HAO2 PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 6.585e-33
Identity = 72/176 (40.91%), Postives = 107/176 (60.80%), Query Frame = 1
Query:  148 EYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGP-GIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKN 672
            ++   A+E L K  +D+   GA+D  T  EN  AF +I  RPR L+DVSK+DM TT+ G  IS PI IAPT F ++A P+GE                S++A+ S+E++ +  P G+R+FQLYV  +R ++ Q++++ E  GFKA+ +TVD P++G R  DI N+  L   L LK+
Sbjct:    8 DFREHAREHLSKSTWDFIEGGADDCCTRDENMAAFKKIRLRPRYLKDVSKVDMRTTIQGAEISAPICIAPTGFHRLAWPDGEMSTARAAQAASICYITSTYASCSLEDIVAAAPRGLRWFQLYVHPNRQINKQMIQKVESLGFKALVITVDVPKVGNRRNDITNQVDLMKKLLLKD 183          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX2_RAT (Hydroxyacid oxidase 2 OS=Rattus norvegicus GN=Hao2 PE=1 SV=2)

HSP 1 Score: 138.272 bits (347), Expect = 4.268e-32
Identity = 74/180 (41.11%), Postives = 110/180 (61.11%), Query Frame = 1
Query:  148 EYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGP-GIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYEGL 684
            +++A A+++L K  +D+    A+D  T  EN  AF RI  RPR LRD+SK+D  TT+ G  IS PI I+PTAF  +A P+GE               +SS+A+ S+E++ +  P G R+FQLY+  D + + Q+V+RAE  GFKA+ +T+DTP LG R  D +N+  L  ++ LK+   L
Sbjct:    8 DFKAHAQKQLSKTSWDFIEGEADDGITYSENIAAFKRIRLRPRYLRDMSKVDTRTTIQGQEISAPICISPTAFHSIAWPDGEKSTARAAQEANICYVISSYASYSLEDIVAAAPEGFRWFQLYMKSDWDFNKQMVQRAEALGFKALVITIDTPVLGNRRRDKRNQLNLEANILLKDLRAL 187          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: HAOX2_HUMAN (Hydroxyacid oxidase 2 OS=Homo sapiens GN=HAO2 PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 8.049e-31
Identity = 74/178 (41.57%), Postives = 107/178 (60.11%), Query Frame = 1
Query:  148 EYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTGP-GIRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRFVLPPHLTLKNYE 678
            +++A A+E+L K   D+   GA+D  T  +N  AF RI  RPR LRDVS++D  TT+ G  IS PI IAPT F  +  P+GE          G     S++A+ S+E++    P G+R+FQLYV  D  ++ QL++R E  GFKA+ +T+DTP  G R  DI+N+  L  +LTL + +
Sbjct:    8 DFQAHAREQLSKSTRDFIEGGADDSITRDDNIAAFKRIRLRPRYLRDVSEVDTRTTIQGEEISAPICIAPTGFHCLVWPDGEMSTARAAQAAGICYITSTFASCSLEDIVIAAPEGLRWFQLYVHPDLQLNKQLIQRVESLGFKALVITLDTPVCGNRRHDIRNQ--LRRNLTLTDLQ 183          
BLAST of FC869021 vs. ExPASy Swiss-Prot
Match: CYB2_HANAN (Cytochrome b2, mitochondrial OS=Hansenula anomala GN=CYB2 PE=1 SV=2)

HSP 1 Score: 133.65 bits (335), Expect = 1.051e-30
Identity = 68/175 (38.86%), Postives = 105/175 (60.00%), Query Frame = 1
Query:  124 ISEITNVMEYEALAKEKLPKMVYDYYASGAEDQWTLQENRNAFSRILFRPRILRDVSKIDMTTTVLGFNISMPIMIAPTAFQKMAHPEGECXXXXXXXXXGTIMTLSSWATSSVEEVSSTG-PG-IRFFQLYVTKDRNVDAQLVKRAERAGFKAIALTVDTPRLGRREADIKNRF 642
            +S++ N+ ++E +A++ LP     YY S A+D+ TL+EN NA+ RI F P+IL DV  +D++T   G   S P  I+ TA  K+ HPEGE            +  +S+ A+ S +E++    PG  +++QLYV  DR++  + V+ AE  G K + +TVD P LGRRE D+K +F
Sbjct:  184 LSQMINLHDFETIARQILPPPALAYYCSAADDEVTLRENHNAYHRIFFNPKILIDVKDVDISTEFFGEKTSAPFYISATALAKLGHPEGEVAIAKGAGREDVVQMISTLASCSFDEIADARIPGQQQWYQLYVNADRSITEKAVRHAEERGMKGLFITVDAPSLGRREKDMKMKF 358          
The following BLAST results are available for this feature:
BLAST of FC869021 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 127
Match NameE-valueIdentityDescription
GOX_SPIOL3.670e-9284.24Peroxisomal (S)-2-hydroxy-acid oxidase OS=Spinacia... [more]
GOX2_ARATH3.107e-9182.76Probable peroxisomal (S)-2-hydroxy-acid oxidase 2 ... [more]
GOX1_ARATH1.305e-8981.28Probable peroxisomal (S)-2-hydroxy-acid oxidase 1 ... [more]
HAOX1_HUMAN1.321e-4951.23Hydroxyacid oxidase 1 OS=Homo sapiens GN=HAO1 PE=1... [more]
HAOX1_MOUSE3.842e-4951.72Hydroxyacid oxidase 1 OS=Mus musculus GN=Hao1 PE=2... [more]
HAOX_DICDI5.193e-4650.23Hydroxyacid oxidase OS=Dictyostelium discoideum GN... [more]
HAOX2_BOVIN6.585e-3340.91Hydroxyacid oxidase 2 OS=Bos taurus GN=HAO2 PE=2 S... [more]
HAOX2_RAT4.268e-3241.11Hydroxyacid oxidase 2 OS=Rattus norvegicus GN=Hao2... [more]
HAOX2_HUMAN8.049e-3141.57Hydroxyacid oxidase 2 OS=Homo sapiens GN=HAO2 PE=2... [more]
CYB2_HANAN1.051e-3038.86Cytochrome b2, mitochondrial OS=Hansenula anomala ... [more]

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Properties
Property NameValue
Genbank descriptionC31007F09EF AbioticL1 Citrus clementina cDNA clone C31007F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC869021 ID=FC869021; Name=FC869021; organism=Citrus clementina; type=EST; length=738bp
GACTCTCTCTTCCTTCAGTCTTCATTTCCCAGTTTCTGACTTTATTGCTT
TGCGCTCCCTTCACTGATTCATTTCTGCTTCTTTTGTTGTTCAAGGAAGC
CAAGCGATAGCATTATGACTTATATATCGGAGATCACCAATGTTATGGAG
TATGAGGCTCTTGCAAAGGAGAAATTGCCGAAGATGGTTTATGACTACTA
TGCATCAGGTGCTGAGGACCAGTGGACTCTTCAGGAAAACCGTAATGCAT
TCTCTAGGATTCTATTTCGACCACGCATTCTTAGAGATGTAAGCAAGATT
GACATGACTACAACAGTCTTGGGCTTCAACATTTCCATGCCCATCATGAT
TGCTCCAACGGCATTTCAGAAAATGGCTCATCCTGAAGGGGAGTGTGCAA
CAGCAAGGGCAGCCTCAGCAGCTGGCACAATCATGACATTATCTTCATGG
GCCACTTCCAGTGTCGAGGAAGTTTCTTCAACGGGACCTGGAATTCGCTT
TTTCCAACTCTATGTGACCAAGGACAGGAATGTGGACGCACAGCTAGTAA
AAAGAGCTGAAAGGGCTGGTTTTAAGGCGATTGCCCTTACCGTCGATACT
CCAAGGCTTGGCCGTCGGGAAGCTGATATTAAGAACAGATTTGTTTTGCC
GCCACATCTGACTTTGAAGAACTATGAGGGCTTGTATATTGGCAAGATGG
ATAAGACTGATGACTCTGGATTAGCTTCGTATGTTGCC
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