FC921302

Overview
NameFC921302
Unique NameFC921302
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length703
Libraries
Library NameType
RVDevelopNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_TOBAC (Proliferating cell nuclear antigen OS=Nicotiana tabacum GN=PCNA PE=2 SV=1)

HSP 1 Score: 373.244 bits (957), Expect = 2.084e-106
Identity = 189/198 (95.45%), Postives = 195/198 (98.48%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE+IK+LVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNL N++KMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEF+RICKDL+SIGDTVVISVTKEGVKFSTRGDIGTANIV RQNTTVDKPEEAT IE
Sbjct:    1 MLELRLVQGSLLKKVLESIKDLVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLANMAKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFSRICKDLSSIGDTVVISVTKEGVKFSTRGDIGTANIVCRQNTTVDKPEEATVIE 198

HSP 2 Score: 32.7278 bits (73), Expect = 2.084e-106
Identity = 14/16 (87.50%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVSLTFALRY+NS
Sbjct:  199 MNEPVSLTFALRYLNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA1_DAUCA (Proliferating cell nuclear antigen OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 3.548e-106
Identity = 186/198 (93.94%), Postives = 195/198 (98.48%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKV+++IK+LVNDANFDCSATGFSLQAMDSSHVALVA+LLRSEGFEHYRCDRNISMGMNL N++KMLKCAGNDDIIT+KADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDL+SIGDTVVISVTKEGVKFSTRGDIGTANIV RQNTTVDKPEEAT IE
Sbjct:    1 MLELRLVQGSLLKKVMDSIKDLVNDANFDCSATGFSLQAMDSSHVALVAVLLRSEGFEHYRCDRNISMGMNLGNMAKMLKCAGNDDIITIKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLSSIGDTVVISVTKEGVKFSTRGDIGTANIVCRQNTTVDKPEEATVIE 198

HSP 2 Score: 33.8834 bits (76), Expect = 3.548e-106
Identity = 15/16 (93.75%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVSLTFALRYMNS
Sbjct:  199 MNEPVSLTFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_CATRO (Proliferating cell nuclear antigen OS=Catharanthus roseus PE=2 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 8.632e-105
Identity = 185/198 (93.43%), Postives = 194/198 (97.98%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE++K+LV DANFDCSA+GFSLQAMDSSHVALVALLLRSEGFEHYRCDRN SMGMNLNN++KMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKI+DFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDL+SIGDTVVISVTKEGVKFSTRGDIGTANIV RQNTTVDKP+EAT IE
Sbjct:    1 MLELRLVQGSLLKKVLESLKDLVTDANFDCSASGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNPSMGMNLNNMAKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKISDFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLSSIGDTVVISVTKEGVKFSTRGDIGTANIVCRQNTTVDKPDEATIIE 198

HSP 2 Score: 32.7278 bits (73), Expect = 8.632e-105
Identity = 14/16 (87.50%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVSLTFALRY+NS
Sbjct:  199 MNEPVSLTFALRYLNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_PEA (Proliferating cell nuclear antigen OS=Pisum sativum GN=PCNA PE=2 SV=1)

HSP 1 Score: 369.392 bits (947), Expect = 1.469e-104
Identity = 184/198 (92.93%), Postives = 196/198 (98.99%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE+IKELVNDANFDCS+TGFSLQAMDSSHVALVALLLRSEGFEHYRCDRN+SMGMNLNN++KMLKCAGNDDIIT+KADDGSDTVTFMFESPTQDKI+DFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDL+SIGDTVVI+V+KEGVKFST+GDIG+ANIV RQNTTVDKPEEAT IE
Sbjct:    1 MLELRLVQGSLLKKVLESIKELVNDANFDCSSTGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNLSMGMNLNNMAKMLKCAGNDDIITIKADDGSDTVTFMFESPTQDKISDFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLSSIGDTVVIAVSKEGVKFSTKGDIGSANIVCRQNTTVDKPEEATVIE 198

HSP 2 Score: 30.4166 bits (67), Expect = 1.469e-104
Identity = 13/16 (81.25%), Postives = 15/16 (93.75%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPV+L FALRYMNS
Sbjct:  199 MNEPVALQFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_POPNI (Proliferating cell nuclear antigen OS=Populus nigra GN=PCNA PE=2 SV=1)

HSP 1 Score: 363.614 bits (932), Expect = 1.234e-103
Identity = 183/198 (92.42%), Postives = 193/198 (97.47%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE+IK+LVNDANFD S++GFSLQ+MDSSHVALVALLL+SEGFEHYRCDRN SMGMNL N+SKMLKCAGNDDIIT+K DDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIV+MPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPE+AT IE
Sbjct:    1 MLELRLVQGSLLKKVLESIKDLVNDANFDFSSSGFSLQSMDSSHVALVALLLKSEGFEHYRCDRNTSMGMNLGNMSKMLKCAGNDDIITIKGDDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVKMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEDATVIE 198

HSP 2 Score: 33.113 bits (74), Expect = 1.234e-103
Identity = 14/16 (87.50%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVS+TFALRYMNS
Sbjct:  199 MNEPVSMTFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA2_ARATH (Proliferating cell nuclear antigen 2 OS=Arabidopsis thaliana GN=At2g29570 PE=1 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 1.610e-103
Identity = 181/198 (91.41%), Postives = 192/198 (96.97%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLEA+K+LVNDANFDCS TGFSLQAMDSSHVALV+LLLRSEGFEHYRCDRN+SMGMNL N+SKMLKCAGNDDIIT+KADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIP+AEYH+IVRMPS EF+RICKDL+SIGDTVVISVTKEGVKFST GDIGTANIVLRQNTTVDKPE+A  IE
Sbjct:    1 MLELRLVQGSLLKKVLEAVKDLVNDANFDCSTTGFSLQAMDSSHVALVSLLLRSEGFEHYRCDRNLSMGMNLGNMSKMLKCAGNDDIITIKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPDAEYHSIVRMPSGEFSRICKDLSSIGDTVVISVTKEGVKFSTAGDIGTANIVLRQNTTVDKPEDAIVIE 198

HSP 2 Score: 32.3426 bits (72), Expect = 1.610e-103
Identity = 14/16 (87.50%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVSL+FALRYMNS
Sbjct:  199 MNEPVSLSFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA1_ARATH (Proliferating cellular nuclear antigen 1 OS=Arabidopsis thaliana GN=PCNA PE=1 SV=2)

HSP 1 Score: 361.303 bits (926), Expect = 1.763e-102
Identity = 180/198 (90.91%), Postives = 192/198 (96.97%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE+IK+LVNDANFDCS+TGFSLQAMDSSHVALV+LLLRSEGFEHYRCDRN+SMGMNL N+SKMLKCAGNDDIIT+KADDG DTVTFMFESPTQDKIADFEMKLMDIDSEHLGIP+AEYH+IVRMPS EF+RICKDL+SIGDTVVISVTKEGVKFST GDIGTANIVLRQNTTVDKPE+A  IE
Sbjct:    1 MLELRLVQGSLLKKVLESIKDLVNDANFDCSSTGFSLQAMDSSHVALVSLLLRSEGFEHYRCDRNLSMGMNLGNMSKMLKCAGNDDIITIKADDGGDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPDAEYHSIVRMPSNEFSRICKDLSSIGDTVVISVTKEGVKFSTAGDIGTANIVLRQNTTVDKPEDAIVIE 198

HSP 2 Score: 31.5722 bits (70), Expect = 1.763e-102
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M EPVSL+FALRYMNS
Sbjct:  199 MKEPVSLSFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_BRANA (Proliferating cell nuclear antigen OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 1.480e-101
Identity = 178/198 (89.90%), Postives = 190/198 (95.96%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLE+IK+LVNDANFDCS TGFSLQAMDSSHVALV+LLLRSEGFEHYRCDRN+SMGMNL N+SKMLKCAGNDDIIT+KADDG DTVTFMFESP QDKIADFEMKLMDIDSEHLGIP+AEYH+IVRMPS EF+RICKDL++IGDTVVISVTKEGVKFST GDIGTANIVLRQNTTVDKPE+A  IE
Sbjct:    1 MLELRLVQGSLLKKVLESIKDLVNDANFDCSTTGFSLQAMDSSHVALVSLLLRSEGFEHYRCDRNLSMGMNLGNMSKMLKCAGNDDIITIKADDGGDTVTFMFESPKQDKIADFEMKLMDIDSEHLGIPDAEYHSIVRMPSNEFSRICKDLSTIGDTVVISVTKEGVKFSTAGDIGTANIVLRQNTTVDKPEDAIVIE 198

HSP 2 Score: 32.3426 bits (72), Expect = 1.480e-101
Identity = 14/16 (87.50%), Postives = 16/16 (100.00%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M+EPVSL+FALRYMNS
Sbjct:  199 MNEPVSLSFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_ORYSJ (Proliferating cell nuclear antigen OS=Oryza sativa subsp. japonica GN=Os02g0805200 PE=2 SV=2)

HSP 1 Score: 356.295 bits (913), Expect = 1.480e-101
Identity = 177/198 (89.39%), Postives = 190/198 (95.96%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLEAI+ELV DANFDCS TGFSLQAMDSSHVALVALLLRSEGFEHYRCDRN+SMGMNLNN++KML+CAGNDDIIT+KADDGSDTVTFMFESP QDKIADFEMKLMDIDSEHLGIP++EY AIVRMPS+EF+RICKDL+SIGDTV+ISVTKEGVKFST GDIGTANIV RQN TVDKPE+AT IE
Sbjct:    1 MLELRLVQGSLLKKVLEAIRELVTDANFDCSGTGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNLSMGMNLNNMAKMLRCAGNDDIITIKADDGSDTVTFMFESPNQDKIADFEMKLMDIDSEHLGIPDSEYQAIVRMPSSEFSRICKDLSSIGDTVIISVTKEGVKFSTAGDIGTANIVCRQNKTVDKPEDATIIE 198

HSP 2 Score: 33.4982 bits (75), Expect = 1.480e-101
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M EPVSLTFALRYMNS
Sbjct:  199 MQEPVSLTFALRYMNS 214
[Close]
BLAST of FC921302 vs. ExPASy Swiss-Prot
Match: PCNA_MAIZE (Proliferating cell nuclear antigen OS=Zea mays GN=PCNA PE=2 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 5.595e-101
Identity = 176/198 (88.89%), Postives = 190/198 (95.96%), Query Frame = 3 
Query:   63 MLELRLVQGSLLKKVLEAIKELVNDANFDCSATGFSLQAMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSAEFARICKDLASIGDTVVISVTKEGVKFSTRGDIGTANIVLRQNTTVDKPEEAT*IE 656
            MLELRLVQGSLLKKVLEAI+ELVNDANFDCS TGFSLQAMDSSHVALVALLLR+EGFEHYRCDRN+SMGMNLNN++KML+CAGNDDIIT+KADDGSDTVTFMFESP QDKIADFEMKLMDIDSEHLGIP++EY AIVRMPS+EF RICKDL+SIGDTVVISVTKEGVKFST G+IG+ANIV RQN T+DKPEEAT IE
Sbjct:    1 MLELRLVQGSLLKKVLEAIRELVNDANFDCSGTGFSLQAMDSSHVALVALLLRAEGFEHYRCDRNLSMGMNLNNMAKMLRCAGNDDIITIKADDGSDTVTFMFESPKQDKIADFEMKLMDIDSEHLGIPDSEYQAIVRMPSSEFMRICKDLSSIGDTVVISVTKEGVKFSTSGEIGSANIVCRQNQTIDKPEEATIIE 198

HSP 2 Score: 33.4982 bits (75), Expect = 5.595e-101
Identity = 15/16 (93.75%), Postives = 15/16 (93.75%), Query Frame = 2 
Query:  656 MHEPVSLTFALRYMNS 703
            M EPVSLTFALRYMNS
Sbjct:  199 MQEPVSLTFALRYMNS 214
The following BLAST results are available for this feature:
BLAST of FC921302 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 54
Match NameE-valueIdentityDescription
PCNA_TOBAC2.084e-10695.45Proliferating cell nuclear antigen OS=Nicotiana ta... [more]
PCNA1_DAUCA3.548e-10693.94Proliferating cell nuclear antigen OS=Daucus carot... [more]
PCNA_CATRO8.632e-10593.43Proliferating cell nuclear antigen OS=Catharanthus... [more]
PCNA_PEA1.469e-10492.93Proliferating cell nuclear antigen OS=Pisum sativu... [more]
PCNA_POPNI1.234e-10392.42Proliferating cell nuclear antigen OS=Populus nigr... [more]
PCNA2_ARATH1.610e-10391.41Proliferating cell nuclear antigen 2 OS=Arabidopsi... [more]
PCNA1_ARATH1.763e-10290.91Proliferating cellular nuclear antigen 1 OS=Arabid... [more]
PCNA_BRANA1.480e-10189.90Proliferating cell nuclear antigen OS=Brassica nap... [more]
PCNA_ORYSJ1.480e-10189.39Proliferating cell nuclear antigen OS=Oryza sativa... [more]
PCNA_MAIZE5.595e-10188.89Proliferating cell nuclear antigen OS=Zea mays GN=... [more]

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Properties
Property NameValue
Genbank descriptionC32103D08EF RVDevelopN Citrus clementina cDNA clone C32103D08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC921302 ID=FC921302; Name=FC921302; organism=Citrus clementina; type=EST; length=703bp
GAAGCTTTCCCTTTCTATCTTTCAAACCCTAACCCTAATTTCCCAAGAAA
CTCGCAGCAAAAATGCTTGAGCTAAGGCTAGTTCAAGGATCCCTTCTCAA
GAAAGTGCTGGAGGCCATCAAGGAGCTGGTCAACGATGCCAACTTTGACT
GCTCGGCCACCGGTTTCTCTCTTCAAGCCATGGACTCCAGCCACGTGGCG
CTGGTGGCCCTGCTGCTCAGATCGGAAGGCTTCGAGCACTACCGCTGTGA
CCGGAATATCTCTATGGGAATGAATCTCAATAACGTGTCGAAGATGCTTA
AGTGTGCTGGCAACGATGACATCATCACTCTCAAGGCCGACGACGGCAGC
GATACCGTTACTTTCATGTTTGAAAGTCCCACTCAAGATAAGATTGCTGA
TTTTGAGATGAAACTGATGGACATTGATAGTGAGCATCTTGGTATACCGG
AGGCTGAATACCATGCCATTGTAAGGATGCCTTCAGCTGAGTTTGCTAGA
ATTTGTAAAGATCTTGCCAGCATTGGTGACACAGTCGTGATTTCTGTCAC
AAAGGAGGGTGTGAAGTTCTCTACCAGAGGTGATATTGGAACTGCTAATA
TTGTGCTCAGGCAAAATACTACAGTAGACAAGCCAGAAGAAGCAACATAA
ATTGAATGCATGAGCCAGTGTCATTGACTTTTGCTCTGAGATACATGAAT
TCA
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