FC931874

Overview
NameFC931874
Unique NameFC931874
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length711
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF2_ARATH (Thioredoxin F-type 2, chloroplastic OS=Arabidopsis thaliana GN=At5g16400 PE=2 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.037e-52
Identity = 99/124 (79.84%), Postives = 110/124 (88.71%), Query Frame = 2
Query:  239 GVFTVRSSLEIAGATVGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRSG 610
            G   VR SLE    TVG+VTEV+KDTFWPIVKAAGDK VVLDMYTQWCGPCKVIAPK++EL++KY D++FLKLDCNQ+NK LAKELGIRVVPTFKILKDNKVVKEVTGAK EDL+ AI+A RSG
Sbjct:   62 GSCVVRCSLETVNVTVGQVTEVDKDTFWPIVKAAGDKIVVLDMYTQWCGPCKVIAPKYKELSEKYQDMVFLKLDCNQDNKPLAKELGIRVVPTFKILKDNKVVKEVTGAKYEDLLAAIEAARSG 185          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF_PEA (Thioredoxin F-type, chloroplastic OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.320e-51
Identity = 99/122 (81.15%), Postives = 112/122 (91.80%), Query Frame = 2
Query:  248 TVRSSLEIAG--ATVGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRS 607
            +VRSSLE AG   TVG+VTEVNKDTFWPIV AAGDKTVVLDM+T+WCGPCKVIAP ++EL++KY DV+FLKLDCNQ+NKSLAKELGI+VVPTFKILKDNK+VKEVTGAK +DLV AID VRS
Sbjct:   60 SVRSSLETAGPTVTVGKVTEVNKDTFWPIVNAAGDKTVVLDMFTKWCGPCKVIAPLYEELSQKYLDVVFLKLDCNQDNKSLAKELGIKVVPTFKILKDNKIVKEVTGAKFDDLVAAIDTVRS 181          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF1_ARATH (Thioredoxin F-type 1, chloroplastic OS=Arabidopsis thaliana GN=At3g02730 PE=1 SV=2)

HSP 1 Score: 193.741 bits (491), Expect = 8.010e-49
Identity = 93/124 (75.00%), Postives = 106/124 (85.48%), Query Frame = 2
Query:  236 GGVFTVRSSLEIAGATVGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRS 607
            G    VR SLE    +VG+VTEV+KDTFWPIVKAAG+K VVLDMYTQWCGPCKVIAPK++ L++KY DV+FLKLDCN +N+ LAKELGIRVVPTFKILKDNKVVKEVTGAK +DLV AI+  RS
Sbjct:   51 GDSSVVRCSLETVNVSVGQVTEVDKDTFWPIVKAAGEKLVVLDMYTQWCGPCKVIAPKYKALSEKYDDVVFLKLDCNPDNRPLAKELGIRVVPTFKILKDNKVVKEVTGAKYDDLVAAIETARS 174          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF_BRANA (Thioredoxin F-type, chloroplastic OS=Brassica napus GN=TRXF PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.330e-48
Identity = 92/124 (74.19%), Postives = 106/124 (85.48%), Query Frame = 2
Query:  236 GGVFTVRSSLEIAGATVGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRS 607
            G    VR SL+    +VG+VTEV+KDTFWPIVKAAG+K VVLDMYTQWCGPCKVIAPK++ L++KY DV+FLKLDCN EN+ LAKELGIRVVPTFKILKDN+VVKEVTGAK +DLV AI+  RS
Sbjct:   54 GDSSVVRCSLQTVNVSVGQVTEVDKDTFWPIVKAAGEKIVVLDMYTQWCGPCKVIAPKYKALSEKYEDVVFLKLDCNPENRPLAKELGIRVVPTFKILKDNQVVKEVTGAKYDDLVAAIETARS 177          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF_SPIOL (Thioredoxin F-type, chloroplastic OS=Spinacia oleracea PE=1 SV=2)

HSP 1 Score: 186.422 bits (472), Expect = 1.279e-46
Identity = 95/138 (68.84%), Postives = 113/138 (81.88%), Query Frame = 2
Query:  212 KKSNKQLNGGVFTVRSSLEIAGAT------VGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRS 607
            +K  +++ GG   VR+S+E A  T      VG+VTEVNKDTFWPIVKAAGDK VVLDM+TQWCGPCK +APK+++LA++Y DVIFLKLDCNQENK+LAKELGIRVVPTFKILK+N VV EVTGAK + L+ AI A RS
Sbjct:   54 RKDRRRMRGG--EVRASMEQALGTQEMEAIVGKVTEVNKDTFWPIVKAAGDKPVVLDMFTQWCGPCKAMAPKYEKLAEEYLDVIFLKLDCNQENKTLAKELGIRVVPTFKILKENSVVGEVTGAKYDKLLEAIQAARS 189          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXF_MESCR (Thioredoxin F-type, chloroplastic OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 7.017e-45
Identity = 93/132 (70.45%), Postives = 107/132 (81.06%), Query Frame = 2
Query:  227 QLNGGVFTVRSSLEIA-GAT----VGEVTEVNKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAIDAVRS 607
            +++G    V++SLE A GA     VG+VTEV+KDTFWPI   AGDK VVLDMYTQWCGPCKV+APK+QELA+K  DV+FLKLDCNQENK LAKELGIRVVPTFKILK  K+V EVTGAK + LV AI+A RS
Sbjct:   59 RMSGRSCCVKASLETAVGAESETLVGKVTEVDKDTFWPIANGAGDKPVVLDMYTQWCGPCKVMAPKYQELAEKLLDVVFLKLDCNQENKPLAKELGIRVVPTFKILKGGKIVDEVTGAKFDKLVAAIEAARS 190          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: THIO_CALJA (Thioredoxin OS=Callithrix jacchus GN=TXN PE=3 SV=3)

HSP 1 Score: 83.5741 bits (205), Expect = 1.167e-15
Identity = 41/97 (42.27%), Postives = 62/97 (63.92%), Query Frame = 2
Query:  305 NKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAID 595
            +KD F   + AAGDK VV+D    WCGPCK+I P F  L++KYS+V+FL++D + + + +A E  ++ +PTF+  K  + V E +GA  E L   I+
Sbjct:    7 SKDAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVVFLEVDVD-DCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATIN 102          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: THIO_HUMAN (Thioredoxin OS=Homo sapiens GN=TXN PE=1 SV=3)

HSP 1 Score: 81.2629 bits (199), Expect = 5.794e-15
Identity = 41/97 (42.27%), Postives = 61/97 (62.89%), Query Frame = 2
Query:  305 NKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAID 595
            +K  F   + AAGDK VV+D    WCGPCK+I P F  L++KYS+VIFL++D + + + +A E  ++ +PTF+  K  + V E +GA  E L   I+
Sbjct:    7 SKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVD-DCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATIN 102          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: TRXH1_ARATH (Thioredoxin H-type 1 OS=Arabidopsis thaliana GN=TRX1 PE=1 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.686e-14
Identity = 41/84 (48.81%), Postives = 57/84 (67.86%), Query Frame = 2
Query:  332 KAAGDKT-VVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDL 580
            KA   KT VV+D    WCGPC+ IAP F +LAKK  +V+FLK+D   E KS+A +  I+ +PTF  LK+ K++ +V GAK ++L
Sbjct:   23 KANESKTLVVVDFTASWCGPCRFIAPFFADLAKKLPNVLFLKVD-TDELKSVASDWAIQAMPTFMFLKEGKILDKVVGAKKDEL 105          
BLAST of FC931874 vs. ExPASy Swiss-Prot
Match: THIO_RABIT (Thioredoxin OS=Oryctolagus cuniculus GN=TXN PE=1 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 2.202e-14
Identity = 37/97 (38.14%), Postives = 62/97 (63.92%), Query Frame = 2
Query:  305 NKDTFWPIVKAAGDKTVVLDMYTQWCGPCKVIAPKFQELAKKYSDVIFLKLDCNQENKSLAKELGIRVVPTFKILKDNKVVKEVTGAKLEDLVFAID 595
            +K  F  ++ +AGDK VV+D    WCGPCK+I P F  L++K+++V+F+++D + + K +A E  ++ +PTF+  K  + V E +GA  E L   I+
Sbjct:    7 SKSAFQEVLDSAGDKLVVVDFSATWCGPCKMIKPFFHALSEKFNNVVFIEVDVD-DCKDIAAECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATIN 102          
The following BLAST results are available for this feature:
BLAST of FC931874 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53
Match NameE-valueIdentityDescription
TRXF2_ARATH2.037e-5279.84Thioredoxin F-type 2, chloroplastic OS=Arabidopsis... [more]
TRXF_PEA1.320e-5181.15Thioredoxin F-type, chloroplastic OS=Pisum sativum... [more]
TRXF1_ARATH8.010e-4975.00Thioredoxin F-type 1, chloroplastic OS=Arabidopsis... [more]
TRXF_BRANA2.330e-4874.19Thioredoxin F-type, chloroplastic OS=Brassica napu... [more]
TRXF_SPIOL1.279e-4668.84Thioredoxin F-type, chloroplastic OS=Spinacia oler... [more]
TRXF_MESCR7.017e-4570.45Thioredoxin F-type, chloroplastic OS=Mesembryanthe... [more]
THIO_CALJA1.167e-1542.27Thioredoxin OS=Callithrix jacchus GN=TXN PE=3 SV=3[more]
THIO_HUMAN5.794e-1542.27Thioredoxin OS=Homo sapiens GN=TXN PE=1 SV=3[more]
TRXH1_ARATH1.686e-1448.81Thioredoxin H-type 1 OS=Arabidopsis thaliana GN=TR... [more]
THIO_RABIT2.202e-1438.14Thioredoxin OS=Oryctolagus cuniculus GN=TXN PE=1 S... [more]

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Properties
Property NameValue
Genbank descriptionC34202B04EF PostHarveN Citrus clementina cDNA clone C34202B04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931874 ID=FC931874; Name=FC931874; organism=Citrus clementina; type=EST; length=711bp
GACGGAACACAAAACACATATTTTTGTTACTTCACTCTCCACCACAAAAA
TCAAAATGTCTTTACTCCAATTCTCCTCCACCACGGTCCGATCTCCGACG
TCGGTACCGTGCTCCGGGAATCACCAGATCACAGCCTCTGCTTTCTCAGC
GGGAGTCTCAAGCTCAAGAACCGGCTCTTACTCCTCGTCATTAGTAGCGA
AGAAGGCGAATAAGAAGAGCAATAAGCAGCTAAACGGCGGTGTTTTCACG
GTGAGATCGAGCTTGGAGATTGCGGGGGCCACAGTGGGAGAGGTCACTGA
AGTAAATAAGGATACTTTTTGGCCCATCGTTAAGGCCGCCGGTGATAAGA
CTGTTGTTCTTGATATGTACACTCAATGGTGTGGTCCTTGTAAAGTGATA
GCTCCAAAATTTCAAGAATTAGCCAAAAAGTACTCCGATGTTATATTTCT
AAAGCTTGATTGCAACCAAGAGAACAAGTCATTGGCGAAAGAGCTTGGAA
TAAGGGTTGTTCCTACTTTCAAGATTCTGAAAGACAATAAGGTTGTAAAG
GAAGTGACGGGGGCCAAATTGGAAGACCTAGTTTTCGCCATTGATGCTGT
TAGATCCGGTTGAAAATTCTTTCCTATTATTTCTCCTGTAAAACTATAGC
AATATGATCAGCAATGTGTACGTGCTTGAGAATAATTGATTGGTCCACTT
GTATGTATTTA
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