CB305012

Overview
NameCB305012
Unique NameCB305012
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length579
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2325 contig Csv1_Contig2325:131..710. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_THEGJ (Elongation factor 1-alpha OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=tuf PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 7.234e-21
Identity = 33/75 (44.00%), Postives = 55/75 (73.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            +F +L+ K+D R+G  +E+ P+F+K GD+ +V + PTK MV+E   + P +GRFA+RDM QTVA G++ +++K +
Sbjct:  354 RFEQLLAKLDPRTGNIVEENPQFIKTGDSAIVILRPTKAMVIEPVKEIPQMGRFAIRDMGQTVAAGMVISIQKAE 428

HSP 2 Score: 41.2022 bits (95), Expect = 7.234e-21
Identity = 17/37 (45.95%), Postives = 24/37 (64.86%), Query Frame = -3 
Query:  443 REAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 553
            R    F +Q+I++NHP  I  GY PVL  HT+ +AV+
Sbjct:  318 RPKDTFKAQIIVLNHPTAITVGYTPVLHAHTTQVAVR 354
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METM7 (Elongation factor 1-alpha OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=tuf PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 9.410e-21
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  220 FSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 441
            F EL  K++  +G+ LE+ P FLK GDA +VK++PTKP+V+E+  + P LGRFA+RDM  TVA G+   V  K+
Sbjct:  354 FMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVIESVKEIPQLGRFAIRDMGMTVAAGMAIQVTAKN 427

HSP 2 Score: 41.2022 bits (95), Expect = 9.410e-21
Identity = 16/38 (42.11%), Postives = 25/38 (65.79%), Query Frame = -3 
Query:  449 DPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 562
            +P   AA+FT+Q++++ HP  +  GY PV   HT+ IA
Sbjct:  314 NPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIA 351
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_CENSY (Elongation factor 1-alpha OS=Cenarchaeum symbiosum GN=tuf PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.224e-20
Identity = 32/72 (44.44%), Postives = 52/72 (72.22%), Query Frame = -1 
Query:  223 SELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 438
            SE V+KI+  +G   E+ PKFLK GD+ ++K+ P +P  +ETF ++P +GRFA+RDM  T+A G++K + ++
Sbjct:  362 SEFVSKINPATGAVEEENPKFLKVGDSAIIKIRPVRPTPIETFKEFPEMGRFALRDMGATIAAGIVKEITEE 433

HSP 2 Score: 43.1282 bits (100), Expect = 1.224e-20
Identity = 15/38 (39.47%), Postives = 26/38 (68.42%), Query Frame = -3 
Query:  449 DPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 562
            +P + A  F +Q+I+++HP  +  GY PV+ CHT+ +A
Sbjct:  321 NPPKVAKEFLAQIIVIHHPTALAPGYTPVMHCHTAQVA 358
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METBF (Elongation factor 1-alpha OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=tuf PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.224e-20
Identity = 36/71 (50.70%), Postives = 50/71 (70.42%), Query Frame = -1 
Query:  223 ELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 435
            EL  K+D +SG+  E+ P FLK GDA +V + PTKPMV+E   + P LGRFA+RDM  T+A G+  +V++K
Sbjct:  352 ELNKKLDPKSGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLGRFAIRDMGMTIAAGMCMSVKQK 422

HSP 2 Score: 43.1282 bits (100), Expect = 1.224e-20
Identity = 17/42 (40.48%), Postives = 25/42 (59.52%), Query Frame = -3 
Query:  440 DDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKV 565
            D+P + A  F  Q++++ HP  I  GY PV   HTS IA ++
Sbjct:  309 DNPPKVADTFVGQIVVLQHPSAITAGYTPVFHAHTSQIACQL 350
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METMP (Elongation factor 1-alpha OS=Methanococcus maripaludis GN=tuf PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.592e-20
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  220 FSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 441
            F EL  K++  +G+ LE+ P FLK GDA +VK++PTKP+V+E+  + P LGRFA+RDM  TVA G+   V  K+
Sbjct:  354 FMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMAIQVTAKN 427

HSP 2 Score: 41.2022 bits (95), Expect = 1.592e-20
Identity = 16/38 (42.11%), Postives = 25/38 (65.79%), Query Frame = -3 
Query:  449 DPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 562
            +P   AA+FT+Q++++ HP  +  GY PV   HT+ IA
Sbjct:  314 NPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIA 351
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METM6 (Elongation factor 1-alpha OS=Methanococcus maripaludis (strain C6 / ATCC BAA-1332) GN=tuf PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.592e-20
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  220 FSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 441
            F EL  K++  +G+ LE+ P FLK GDA +VK++PTKP+V+E+  + P LGRFA+RDM  TVA G+   V  K+
Sbjct:  354 FMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMAIQVTAKN 427

HSP 2 Score: 41.2022 bits (95), Expect = 1.592e-20
Identity = 16/38 (42.11%), Postives = 25/38 (65.79%), Query Frame = -3 
Query:  449 DPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 562
            +P   AA+FT+Q++++ HP  +  GY PV   HT+ IA
Sbjct:  314 NPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIA 351
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METM5 (Elongation factor 1-alpha OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=tuf PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.592e-20
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = -1 
Query:  220 FSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 441
            F EL  K++  +G+ LE+ P FLK GDA +VK++PTKP+V+E+  + P LGRFA+RDM  TVA G+   V  K+
Sbjct:  354 FMELQKKLNPATGEVLEENPDFLKAGDAAIVKLMPTKPLVMESVKEIPQLGRFAIRDMGMTVAAGMAIQVTAKN 427

HSP 2 Score: 41.2022 bits (95), Expect = 1.592e-20
Identity = 16/38 (42.11%), Postives = 25/38 (65.79%), Query Frame = -3 
Query:  449 DPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 562
            +P   AA+FT+Q++++ HP  +  GY PV   HT+ IA
Sbjct:  314 NPPTVAADFTAQIVVLQHPSVMTVGYTPVFHAHTAQIA 351
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_CALMQ (Elongation factor 1-alpha OS=Caldivirga maquilingensis (strain DSMZ 13496 / IC-167) GN=tuf PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.502e-20
Identity = 36/76 (47.37%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  229 TLPSKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVE 456
            T+P++  EL++++D  +G+ +E++P+F+K GD  MV++ P KP+VVE FSD P LGRF++RDM +TVA G I  ++
Sbjct:  361 TVPTQIIELISRLDPATGQTVEQKPQFIKRGDVAMVRLKPLKPVVVERFSDLPALGRFSLRDMGRTVAAGQIIEIK 436

HSP 2 Score: 34.6538 bits (78), Expect = 3.502e-20
Identity = 12/42 (28.57%), Postives = 24/42 (57.14%), Query Frame = -3 
Query:  440 DDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKV 565
            ++P   A    +++ ++ HP  IG GY PV+  HT+ +  ++
Sbjct:  325 NNPPTVAEEIVARIAVLEHPTTIGVGYTPVMHVHTATVPTQI 366
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_AERPE (Elongation factor 1-alpha OS=Aeropyrum pernix GN=tuf PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.002e-19
Identity = 35/76 (46.05%), Postives = 57/76 (75.00%), Query Frame = -1 
Query:  229 TLPSKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVE 456
            ++ S+  E+  K+D ++G+ +E+ P+FLK GDA +V+  P KP+VVE FS+ P LGRFA+RDM +TV +G++ +V+
Sbjct:  353 SVSSRIIEIKAKLDPKTGQVVEQNPQFLKAGDAAIVRFKPVKPLVVEKFSEIPQLGRFAMRDMNRTVGIGIVTDVK 428

HSP 2 Score: 34.6538 bits (78), Expect = 1.002e-19
Identity = 13/42 (30.95%), Postives = 24/42 (57.14%), Query Frame = -3 
Query:  440 DDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKV 565
            D P   A  F +++ ++ HP  I  GY PV+  HT+ ++ ++
Sbjct:  317 DKPPTVAEEFEARIFVIWHPSAITVGYTPVIHVHTASVSSRI 358
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_METMA (Elongation factor 1-alpha OS=Methanosarcina mazei GN=tuf PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.003e-19
Identity = 33/67 (49.25%), Postives = 48/67 (71.64%), Query Frame = -1 
Query:  223 KIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 423
            K+D ++G+  E+ P FLK GDA +V + PTKPMV+E   + P LGRFA+RDM  T+A G+  +V++K
Sbjct:  356 KLDPKTGQVKEENPTFLKAGDAAIVTIKPTKPMVIEPVKEIPQLGRFAIRDMGMTIAAGMCMSVKQK 422

HSP 2 Score: 43.1282 bits (100), Expect = 1.003e-19
Identity = 17/42 (40.48%), Postives = 25/42 (59.52%), Query Frame = -3 
Query:  440 DDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKV 565
            D+P + A  F  Q++++ HP  I  GY PV   HTS IA ++
Sbjct:  309 DNPPKVADEFVGQIVVLQHPSAITAGYTPVFHAHTSQIACQL 350
The following BLAST results are available for this feature:
BLAST of CB305012 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A_THEGJ7.234e-2144.00Elongation factor 1-alpha OS=Thermococcus gammatol... [more]
EF1A_METM79.410e-2150.00Elongation factor 1-alpha OS=Methanococcus maripal... [more]
EF1A_CENSY1.224e-2044.44Elongation factor 1-alpha OS=Cenarchaeum symbiosum... [more]
EF1A_METBF1.224e-2050.70Elongation factor 1-alpha OS=Methanosarcina barker... [more]
EF1A_METMP1.592e-2050.00Elongation factor 1-alpha OS=Methanococcus maripal... [more]
EF1A_METM61.592e-2050.00Elongation factor 1-alpha OS=Methanococcus maripal... [more]
EF1A_METM51.592e-2050.00Elongation factor 1-alpha OS=Methanococcus maripal... [more]
EF1A_CALMQ3.502e-2047.37Elongation factor 1-alpha OS=Caldivirga maquilinge... [more]
EF1A_AERPE1.002e-1946.05Elongation factor 1-alpha OS=Aeropyrum pernix GN=t... [more]
EF1A_METMA1.003e-1949.25Elongation factor 1-alpha OS=Methanosarcina mazei ... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IIaR_G01 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IIaR_G01 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB305012 ID=CB305012; Name=CB305012; organism=Citrus sinensis; type=EST; length=579bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAACTTACCAAAAAAAAAC
TCTGATATAAAACCAAATTCAGAGAATTAAAATGAAGTCCCGTAAAACAA
ACTAAAACACGACAAAAATAGCATAAACAGAGAAAATAAAAGCTAACTAT
AGCAGATAGATCCCAAAAAACTCCTCACTTCTTCTTGGCTGCAGACTTGG
TGACCTTGGCACCTGTTGGATCCTTCTTTTCAACATTCTTAATCACACCA
ACAGCAACAGTCTGACGCATGTCCCTAACGGCAAAACGACCAAGAGGTGG
GTAGTCAGAGAAGGTTTCAACAACCATGGGCTTGGTGGGAATCATCTTAA
CCATTCCAGCATCTCCATTCTTCAAGAACTTGGGCTCCTTCTCAAGCTCC
TTGCCTGATCGCCTATCAATCTTCGTCACGAGCTCAGAAAACTTTGACGG
CAATGTGGGAGGTGTGGCAGTCAAGCACTGGGGCATATCCATTTCCAATC
TGGCCAGGGTGGTTCATGATGATGACCTGTGAGGTGAAGTTGGCAGCTTC
CCTGGCTGGATCATCCTTCGAGTTGGAAG
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