CB305012

Overview
NameCB305012
Unique NameCB305012
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length579
Libraries
Library NameType
Flavedo Maturecdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2325 contig Csv1_Contig2325:131..710. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A2_RAT (Elongation factor 1-alpha 2 OS=Rattus norvegicus GN=Eef1a2 PE=2 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.419e-42
Identity = 58/74 (78.38%), Postives = 65/74 (87.84%), Query Frame = -1 
Query:  223 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 444
            KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIKNVEKK
Sbjct:  371 KFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 73.1738 bits (178), Expect = 3.419e-42
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA K
Sbjct:  328 DSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A2_MOUSE (Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef1a2 PE=1 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.419e-42
Identity = 58/74 (78.38%), Postives = 65/74 (87.84%), Query Frame = -1 
Query:  223 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 444
            KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIKNVEKK
Sbjct:  371 KFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKK 444

HSP 2 Score: 73.1738 bits (178), Expect = 3.419e-42
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK DP +EAA FTSQVII+NHPGQI  GY+PV+DCHT+HIA K
Sbjct:  328 DSKADPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_DANRE (Elongation factor 1-alpha OS=Danio rerio GN=eef1a PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 3.419e-42
Identity = 57/74 (77.03%), Postives = 65/74 (87.84%), Query Frame = -1 
Query:  223 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 444
            KF+EL  KIDRRSGK+LE  PK LK+GDA +V+M+P KPM VE+FS YPPLGRFAVRDMRQTVAVGVIK+VEKK
Sbjct:  371 KFAELKEKIDRRSGKKLEDNPKALKSGDAAIVEMVPGKPMCVESFSTYPPLGRFAVRDMRQTVAVGVIKSVEKK 444

HSP 2 Score: 75.485 bits (184), Expect = 3.419e-42
Identity = 33/44 (75.00%), Postives = 38/44 (86.36%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP  EAANF +QVII+NHPGQI  GYAPVLDCHT+HIA K
Sbjct:  328 DSKNDPPMEAANFNAQVIILNHPGQISQGYAPVLDCHTAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A4_OSCTI (Elongation factor 1-alpha 4 OS=Oscheius tipulae GN=eft-4 PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.419e-42
Identity = 54/75 (72.00%), Postives = 65/75 (86.67%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF+EL  K+DRR+GK++E  PKFLK+GDAG+V++IPTKP+ VE F+DY PLGRFAVRDMRQTVAVGVIK V K D
Sbjct:  371 KFAELKEKVDRRTGKKVEDLPKFLKSGDAGIVELIPTKPLCVEAFTDYAPLGRFAVRDMRQTVAVGVIKGVTKDD 445

HSP 2 Score: 77.7962 bits (190), Expect = 3.419e-42
Identity = 33/45 (73.33%), Postives = 41/45 (91.11%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK+DPA+E+ +FT+QVI+MNHPGQIG GY PVLDCHT+HIA K
Sbjct:  327 SDSKNDPAKESKSFTAQVIVMNHPGQIGAGYTPVLDCHTAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A1_OSCTI (Elongation factor 1-alpha 1 OS=Oscheius tipulae GN=eft-1 PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 3.419e-42
Identity = 54/75 (72.00%), Postives = 65/75 (86.67%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF+EL  K+DRR+GK++E  PKFLK+GDAG+V++IPTKP+ VE F+DY PLGRFAVRDMRQTVAVGVIK V K D
Sbjct:  371 KFAELKEKVDRRTGKKVEDLPKFLKSGDAGIVELIPTKPLCVEAFTDYAPLGRFAVRDMRQTVAVGVIKGVTKDD 445

HSP 2 Score: 77.7962 bits (190), Expect = 3.419e-42
Identity = 33/45 (73.33%), Postives = 41/45 (91.11%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK+DPA+E+ +FT+QVI+MNHPGQIG GY PVLDCHT+HIA K
Sbjct:  327 SDSKNDPAKESKSFTAQVIVMNHPGQIGAGYTPVLDCHTAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_TRIRE (Elongation factor 1-alpha OS=Trichoderma reesei GN=tef1 PE=3 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 7.565e-42
Identity = 55/73 (75.34%), Postives = 64/73 (87.67%), Query Frame = -1 
Query:  226 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEK 444
            KF+EL+ KIDRR+GK  E  PKF+K+GD+ +VKMIP+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK VEK
Sbjct:  370 KFAELLEKIDRRTGKATESAPKFIKSGDSAIVKMIPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEK 442

HSP 2 Score: 74.7146 bits (182), Expect = 7.565e-42
Identity = 32/44 (72.73%), Postives = 39/44 (88.64%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP   AA+FT+QVI+MNHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPMGAASFTAQVIVMNHPGQVGAGYAPVLDCHTAHIACK 370
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A3_OSCTI (Elongation factor 1-alpha 3 OS=Oscheius tipulae GN=eft-3 PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 7.565e-42
Identity = 54/75 (72.00%), Postives = 64/75 (85.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KF+EL  K+DRR+GK  E  PKFLK+GDAG+V++IPTKP  VE+F+DY PLGRFAVRDMRQTVAVGVIK+V K D
Sbjct:  372 KFAELKEKVDRRTGKSTEASPKFLKSGDAGIVELIPTKPPCVESFTDYAPLGRFAVRDMRQTVAVGVIKSVTKDD 446

HSP 2 Score: 77.411 bits (189), Expect = 7.565e-42
Identity = 33/45 (73.33%), Postives = 40/45 (88.89%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK+DPA+E+ NFT+QVI+MNHPGQI  GY PVLDCHT+HIA K
Sbjct:  328 SDSKNDPAKESKNFTAQVIVMNHPGQISAGYTPVLDCHTAHIACK 372
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A3_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-3 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 7.565e-42
Identity = 55/75 (73.33%), Postives = 65/75 (86.67%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSEL+ KIDRRS K +E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGVIK VEK D
Sbjct:  369 KFSELIEKIDRRSEK-MEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGVIKAVEKVD 442

HSP 2 Score: 78.1814 bits (191), Expect = 7.565e-42
Identity = 34/45 (75.56%), Postives = 42/45 (93.33%), Query Frame = -3 
Query:  443 SNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 577
            S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA K
Sbjct:  325 SDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACK 369
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A3_HUMAN (Putative elongation factor 1-alpha-like 3 OS=Homo sapiens GN=EEF1AL3 PE=5 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 9.856e-42
Identity = 58/74 (78.38%), Postives = 65/74 (87.84%), Query Frame = -1 
Query:  223 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKK 444
            KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGVIK V+KK
Sbjct:  371 KFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKK 444

HSP 2 Score: 72.0182 bits (175), Expect = 9.856e-42
Identity = 32/44 (72.73%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCH +HIA K
Sbjct:  328 DSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHMAHIACK 371
[Close]
BLAST of CB305012 vs. ExPASy Swiss-Prot
Match: EF1A_COCIM (Elongation factor 1-alpha OS=Coccidioides immitis GN=TEF PE=2 SV=2)

HSP 1 Score: 120.939 bits (302), Expect = 9.857e-42
Identity = 56/75 (74.67%), Postives = 67/75 (89.33%), Query Frame = -1 
Query:  220 KFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGVIKNVEKKD 444
            KFSEL+ KIDRR+GK +E  PKF+K+GDA +VKM+P+KPM VE F+DYPPLGRFAVRDMRQTVAVGVIK+VEK +
Sbjct:  370 KFSELLEKIDRRTGKSVENNPKFIKSGDAAIVKMVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVEKSE 444

HSP 2 Score: 68.9366 bits (167), Expect = 9.857e-42
Identity = 28/44 (63.64%), Postives = 37/44 (84.09%), Query Frame = -3 
Query:  443 NSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVK 574
            +SK+DP +   +F +QVI++NHPGQ+G GYAPVLDCHT+HIA K
Sbjct:  327 DSKNDPPKGCDSFNAQVIVLNHPGQVGAGYAPVLDCHTAHIACK 370
The following BLAST results are available for this feature:
BLAST of CB305012 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 158
Match NameE-valueIdentityDescription
EF1A2_RAT3.419e-4278.38Elongation factor 1-alpha 2 OS=Rattus norvegicus G... [more]
EF1A2_MOUSE3.419e-4278.38Elongation factor 1-alpha 2 OS=Mus musculus GN=Eef... [more]
EF1A_DANRE3.419e-4277.03Elongation factor 1-alpha OS=Danio rerio GN=eef1a ... [more]
EF1A4_OSCTI3.419e-4272.00Elongation factor 1-alpha 4 OS=Oscheius tipulae GN... [more]
EF1A1_OSCTI3.419e-4272.00Elongation factor 1-alpha 1 OS=Oscheius tipulae GN... [more]
EF1A_TRIRE7.565e-4275.34Elongation factor 1-alpha OS=Trichoderma reesei GN... [more]
EF1A3_OSCTI7.565e-4272.00Elongation factor 1-alpha 3 OS=Oscheius tipulae GN... [more]
EF1A3_RHIRA7.565e-4273.33Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A3_HUMAN9.856e-4278.38Putative elongation factor 1-alpha-like 3 OS=Homo ... [more]
EF1A_COCIM9.857e-4274.67Elongation factor 1-alpha OS=Coccidioides immitis ... [more]

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Properties
Property NameValue
Genbank descriptionFLAVEDO0003_IIaR_G01 Flavedo Mature Citrus sinensis cDNA clone FLAVEDO0003_IIaR_G01 3, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CB305012 ID=CB305012; Name=CB305012; organism=Citrus sinensis; type=EST; length=579bp
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAACTTACCAAAAAAAAAC
TCTGATATAAAACCAAATTCAGAGAATTAAAATGAAGTCCCGTAAAACAA
ACTAAAACACGACAAAAATAGCATAAACAGAGAAAATAAAAGCTAACTAT
AGCAGATAGATCCCAAAAAACTCCTCACTTCTTCTTGGCTGCAGACTTGG
TGACCTTGGCACCTGTTGGATCCTTCTTTTCAACATTCTTAATCACACCA
ACAGCAACAGTCTGACGCATGTCCCTAACGGCAAAACGACCAAGAGGTGG
GTAGTCAGAGAAGGTTTCAACAACCATGGGCTTGGTGGGAATCATCTTAA
CCATTCCAGCATCTCCATTCTTCAAGAACTTGGGCTCCTTCTCAAGCTCC
TTGCCTGATCGCCTATCAATCTTCGTCACGAGCTCAGAAAACTTTGACGG
CAATGTGGGAGGTGTGGCAGTCAAGCACTGGGGCATATCCATTTCCAATC
TGGCCAGGGTGGTTCATGATGATGACCTGTGAGGTGAAGTTGGCAGCTTC
CCTGGCTGGATCATCCTTCGAGTTGGAAG
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