CF509770

Overview
NameCF509770
Unique NameCF509770
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length462
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_SOLLC (Delta-1-pyrroline-5-carboxylate synthetase OS=Solanum lycopersicum GN=PRO2 PE=2 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 3.992e-65
Identity = 120/153 (78.43%), Postives = 134/153 (87.58%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIK STKIPVLGHADGICHVYVDKSA++D+AK I +DAKIDYPAACNAMETLLVHKDLA NG LN+L+VELQ +GV L+GGP+AS LL IPE R F HEY+S+ CTVE+V+DV AAIDHIHQHGSAHTD I  EDQ+VAE FL QVDSAAV
Sbjct:  496 VSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAACNAMETLLVHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMIPEARTFRHEYSSLACTVEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVAEVFLRQVDSAAV 648          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_ACTDE (Delta-1-pyrroline-5-carboxylate synthetase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 2.861e-63
Identity = 117/153 (76.47%), Postives = 132/153 (86.27%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIKESTKIPVLGHADGICHVYVDKSAN+D+AK +V+DAK DYPAACNAMETLLVHKDL  NG L+EL+VELQ +GV + GGPRAS LL IPE R  HHEY+S+ CT+EIVDDV AAIDHIH+HGSAHTD I  ED +VAE FL QVDS++V
Sbjct:  496 VSQIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAACNAMETLLVHKDLVQNGCLDELIVELQIKGVVIHGGPRASSLLHIPEARSLHHEYSSLACTIEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVAEIFLRQVDSSSV 648          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS2_ARATH (Delta-1-pyrroline-5-carboxylate synthetase B OS=Arabidopsis thaliana GN=P5CSB PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 5.395e-62
Identity = 117/153 (76.47%), Postives = 128/153 (83.66%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIK STKIPVLGHADGICHVYVDKS  LD+AK IV DAK+DYPAACNAMETLLVHKDL  NG L++L+  LQ +GV L+GGPRAS  L IPET+ FHHEY+S  CTVEIV+DV  AIDHIHQHGSAHTDCI  ED +VAE FL QVDSAAV
Sbjct:  496 VSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACTVEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAV 648          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS1_ARATH (Delta-1-pyrroline-5-carboxylate synthetase A OS=Arabidopsis thaliana GN=P5CSA PE=1 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 8.614e-60
Identity = 111/153 (72.55%), Postives = 126/153 (82.35%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            V+QIK +TKIPVLGHADGICHVYVDK+ + D+AK IV DAK+DYPAACNAMETLLVHKDL  N  LNEL+  LQ  GV L+GGPRASK+L IPE R F+HEY +  CTVE+V+DV  AIDHIH+HGSAHTDCI  ED +VAE FL QVDSAAV
Sbjct:  496 VTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAV 648          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_MESCR (Delta-1-pyrroline-5-carboxylate synthetase OS=Mesembryanthemum crystallinum GN=P5CS PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.125e-59
Identity = 116/155 (74.84%), Postives = 130/155 (83.87%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKL--LQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIKEST+IPVLGHADGICHVYVDKSAN+D+AK IV+DAK DYPAACNAMETLLVHKDLA NG LN+L+V+L+ EGV +FGGPR   L    I  T+ F+ EY+S  CTVEIVDDV AAI+HI+ HGSAHTDCI AED KVAETFL  VDSAAV
Sbjct:  493 VSQIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAACNAMETLLVHKDLAENGGLNDLIVDLRTEGVTMFGGPRIDALQEFNIQATQTFNREYSSPACTVEIVDDVYAAIEHINHHGSAHTDCIIAEDHKVAETFLQLVDSAAV 647          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_ORYSJ (Delta-1-pyrroline-5-carboxylate synthetase OS=Oryza sativa subsp. japonica GN=P5CS PE=2 SV=2)

HSP 1 Score: 225.328 bits (573), Expect = 9.524e-59
Identity = 109/153 (71.24%), Postives = 130/153 (84.97%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIK STKIPVLGHADGICHVY+DKSA++D+AK+IV+DAKIDYPAACNAMETLLVHKDL  +  L++++V L+ EGV ++GGP A K L  P+   FHHEY+SM CTVE VDDV++AIDHIH++GSAHTDCI   D KVAETFL +VDSAAV
Sbjct:  496 VSQIKASTKIPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAACNAMETLLVHKDLMKSPGLDDILVALKTEGVNIYGGPIAHKALGFPKAVSFHHEYSSMACTVEFVDDVQSAIDHIHRYGSAHTDCIVTTDDKVAETFLRRVDSAAV 648          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_VIGAC (Delta-1-pyrroline-5-carboxylate synthetase OS=Vigna aconitifolia PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 2.122e-58
Identity = 110/153 (71.90%), Postives = 128/153 (83.66%), Query Frame = 2
Query:    2 VSQIKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            VSQIK STKIPVLGHADGICHVYVDKSAN+++AK IV+DAK+DYPAACNAMETLL+HKDL   G L E++++L+ EGV L+GGP AS LL IP+   FHHEY+S+ CT EIVDDV AAIDHI+ +GSAHTD I AED +VA  FL QVDSAAV
Sbjct:  497 VSQIKSSTKIPVLGHADGICHVYVDKSANVEMAKRIVLDAKVDYPAACNAMETLLIHKDLIEKGWLKEIILDLRTEGVILYGGPVASSLLNIPQAHSFHHEYSSLACTAEIVDDVYAAIDHINLYGSAHTDSIVAEDNEVANVFLRQVDSAAV 649          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_HUMAN (Delta-1-pyrroline-5-carboxylate synthetase OS=Homo sapiens GN=ALDH18A1 PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 5.262e-33
Identity = 70/156 (44.87%), Postives = 103/156 (66.03%), Query Frame = 2
Query:    2 VSQIKESTK-IPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQI--PETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            V  I+++ K IPV+GH++GICH+YVD  A++D    +V D+K +YPAACNA+ETLL+H+DL      ++++  L+ E V +  GP+ +  L     E +    EY  +   +E+VD+V+ AIDHIH++GS+HTD I  ED+  AE FL  VDSA V
Sbjct:  564 VRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACV 719          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: PROA_SYNPW (Gamma-glutamyl phosphate reductase OS=Synechococcus sp. (strain WH7803) GN=proA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 2.000e-32
Identity = 72/153 (47.06%), Postives = 101/153 (66.01%), Query Frame = 2
Query:   11 IKESTKIPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQIPETRL---FHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            I+++T+IPVLGHADGICH+YVD +A+LD A  + +D+K  YPAACNA+ETLLVH+ +A +  L+  +   +  GV +  G  AS  L +PE+     +  EY  +   V++VDD+ AA DHI + GS HT+CI  ED   A+ FL  +DSA V
Sbjct:  216 IQDNTRIPVLGHADGICHLYVDAAADLDKAVRVALDSKTQYPAACNAIETLLVHRSVAAS-FLSAAIPAFRDAGV-VLRGDAASVALGVPESATEADWRTEYLDLTLAVKVVDDLAAAADHIRRFGSRHTECIVTEDAAAADRFLSSIDSAGV 366          
BLAST of CF509770 vs. ExPASy Swiss-Prot
Match: P5CS_PONAB (Delta-1-pyrroline-5-carboxylate synthetase OS=Pongo abelii GN=ALDH18A1 PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 2.000e-32
Identity = 69/156 (44.23%), Postives = 103/156 (66.03%), Query Frame = 2
Query:    2 VSQIKESTK-IPVLGHADGICHVYVDKSANLDVAKNIVIDAKIDYPAACNAMETLLVHKDLACNGALNELVVELQHEGVGLFGGPRASKLLQI--PETRLFHHEYNSMVCTVEIVDDVRAAIDHIHQHGSAHTDCIGAEDQKVAETFLCQVDSAAV 460
            V  I+++ K IPV+GH++GICH+YVD  A++D    +V D+K +YPAACNA+ETLL+H+DL      ++++  L+ E V +  GP+ +  L     E +    EY  +   +E+VD+V+ AIDHIH++GS+HTD I  E++  AE FL  VDSA V
Sbjct:  564 VRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTENENTAEFFLQHVDSACV 719          
The following BLAST results are available for this feature:
BLAST of CF509770 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
P5CS_SOLLC3.992e-6578.43Delta-1-pyrroline-5-carboxylate synthetase OS=Sola... [more]
P5CS_ACTDE2.861e-6376.47Delta-1-pyrroline-5-carboxylate synthetase OS=Acti... [more]
P5CS2_ARATH5.395e-6276.47Delta-1-pyrroline-5-carboxylate synthetase B OS=Ar... [more]
P5CS1_ARATH8.614e-6072.55Delta-1-pyrroline-5-carboxylate synthetase A OS=Ar... [more]
P5CS_MESCR1.125e-5974.84Delta-1-pyrroline-5-carboxylate synthetase OS=Mese... [more]
P5CS_ORYSJ9.524e-5971.24Delta-1-pyrroline-5-carboxylate synthetase OS=Oryz... [more]
P5CS_VIGAC2.122e-5871.90Delta-1-pyrroline-5-carboxylate synthetase OS=Vign... [more]
P5CS_HUMAN5.262e-3344.87Delta-1-pyrroline-5-carboxylate synthetase OS=Homo... [more]
PROA_SYNPW2.000e-3247.06Gamma-glutamyl phosphate reductase OS=Synechococcu... [more]
P5CS_PONAB2.000e-3244.23Delta-1-pyrroline-5-carboxylate synthetase OS=Pong... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-278 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-81_B04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF509770 ID=CF509770; Name=CF509770; organism=Citrus sinensis; type=EST; length=462bp
TGTTTCTCAAATCAAGGAGTCAACAAAAATTCCTGTACTAGGTCATGCTG
ATGGAATTTGTCATGTTTATGTTGATAAATCGGCTAACCTTGATGTGGCA
AAGAACATTGTTATTGATGCGAAGATAGACTATCCTGCAGCCTGCAATGC
TATGGAAACTCTTCTGGTACACAAGGATTTAGCATGCAATGGTGCACTCA
ATGAGCTTGTTGTAGAACTTCAACATGAAGGTGTCGGTTTATTTGGCGGA
CCAAGAGCAAGTAAATTACTACAAATTCCTGAGACACGCTTATTCCATCA
TGAGTACAACTCAATGGTTTGTACGGTTGAAATTGTGGACGATGTGCGTG
CTGCTATTGACCATATTCATCAACATGGAAGTGCCCATACTGATTGCATT
GGTGCAGAAGATCAAAAAGTTGCTGAAACATTTTTATGTCAAGTTGACAG
TGCTGCAGTATT
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