CF832440

Overview
NameCF832440
Unique NameCF832440
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length794
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF_ORYSJ (Fructose-bisphosphate aldolase cytoplasmic isozyme OS=Oryza sativa subsp. japonica GN=FBA PE=1 SV=2)

HSP 1 Score: 349.747 bits (896), Expect = 1.049e-95
Identity = 171/197 (86.80%), Postives = 187/197 (94.92%), Query Frame = 2
Query:    5 NANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            NA GLARYAIICQENGLVPIVEPEILVDGPHDI++CA V+E VLAACYKALN+HHVLLEGTLLKPNMVTPGS+A KV+P+VIAE+TVR LQRT+PAAVPA+VFLSGGQSEEEAT NLNAMN+L TKKPWSLSFSFGRALQQSTLKAW+GK EN++KA+AAFL RCKANSEATLGTYKGDA LGEGA+ESLHVKDYKY
Sbjct:  162 NAQGLARYAIICQENGLVPIVEPEILVDGPHDIDRCAYVSEVVLAACYKALNEHHVLLEGTLLKPNMVTPGSDAKKVSPEVIAEYTVRTLQRTVPAAVPAIVFLSGGQSEEEATLNLNAMNKLSTKKPWSLSFSFGRALQQSTLKAWSGKAENIEKARAAFLTRCKANSEATLGTYKGDAVLGEGASESLHVKDYKY 358          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF_CICAR (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Cicer arietinum GN=ALDC PE=2 SV=1)

HSP 1 Score: 340.502 bits (872), Expect = 6.365e-93
Identity = 168/198 (84.85%), Postives = 185/198 (93.43%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENA GLARYA+ICQENGLVPIVE EILVDG HDI+KCA +TERVLAA YKAL+DHHVLLEGTLLKPNMVTPGS++PKV P+V+AEHTVRALQRT+PAAVPAVVFLSGGQSEEEAT NLNA+NQ+K KKPW+LSFSFGRALQQSTLKAW+GKEENV+ AQ A L R KANSEATLGTYKG++QLGEGA+ESLHVKDYKY
Sbjct:  162 ENAYGLARYAVICQENGLVPIVELEILVDGSHDIHKCAAITERVLAATYKALSDHHVLLEGTLLKPNMVTPGSDSPKVAPEVVAEHTVRALQRTVPAAVPAVVFLSGGQSEEEATVNLNAINQVKGKKPWTLSFSFGRALQQSTLKAWSGKEENVKNAQDALLTRAKANSEATLGTYKGNSQLGEGASESLHVKDYKY 359          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF_MAIZE (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Zea mays PE=2 SV=1)

HSP 1 Score: 336.265 bits (861), Expect = 1.200e-91
Identity = 168/197 (85.28%), Postives = 181/197 (91.88%), Query Frame = 2
Query:    5 NANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            NA GLARYAIICQENGLVPIVEPEILVDGPHDI++CA VTE VLAACYKALN+HHVLLEGTLLKPNMVTPGS++ KVTP+VIAE+TVR LQRT+PAAVPAV+FLSGGQSEEEATRNLNAMN+L TKKPWSLSFSFGRALQ STLKAWAGK EN++KA+AAFL RCKANSEATLGTYKGDA       ESLHVKDYKY
Sbjct:  162 NAQGLARYAIICQENGLVPIVEPEILVDGPHDIDRCAYVTETVLAACYKALNEHHVLLEGTLLKPNMVTPGSDSKKVTPEVIAEYTVRTLQRTVPAAVPAVLFLSGGQSEEEATRNLNAMNKLSTKKPWSLSFSFGRALQASTLKAWAGKVENLEKARAAFLARCKANSEATLGTYKGDA---AADTESLHVKDYKY 355          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF2_PEA (Fructose-bisphosphate aldolase, cytoplasmic isozyme 2 OS=Pisum sativum PE=3 SV=1)

HSP 1 Score: 333.954 bits (855), Expect = 5.957e-91
Identity = 166/198 (83.84%), Postives = 180/198 (90.91%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENA GLARYA+ICQENGLVPIVEPEILVDG HDI KCA +TERVLAA YKAL+DHHV+LEGTLLKPNMVTPGS+APKV P+VIAEHTVRALQRT+PAAVPAVVFLSGGQSEEEA+ NLNA+NQ+K KKPW+LSFSFGRALQQSTLKAW GK ENV+ AQ A L R KANSEATLGTYKG + LG GA+ESLHVKDYKY
Sbjct:  162 ENAYGLARYAVICQENGLVPIVEPEILVDGSHDILKCAAITERVLAATYKALSDHHVILEGTLLKPNMVTPGSDAPKVAPEVIAEHTVRALQRTVPAAVPAVVFLSGGQSEEEASVNLNAINQIKGKKPWTLSFSFGRALQQSTLKAWGGKTENVKAAQDALLTRAKANSEATLGTYKGASNLGAGASESLHVKDYKY 359          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF_SPIOL (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 323.553 bits (828), Expect = 8.051e-88
Identity = 161/198 (81.31%), Postives = 177/198 (89.39%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENANGLARY IICQENGLVPIVEPEILVDG HDI++CA+V+ERVLAACYKALNDHHVLLEGT LKPN+VTPGSE+ KVTP+VIAE+TVR LQRT+P AVP V+FLSGGQSEEEAT NLNAMN+L+TKKPW+LSFS+GRALQQSTLKAW GKEENV KAQ  FL R K NSEATLG Y+G A  G  A+ESLHVKDYKY
Sbjct:  161 ENANGLARYGIICQENGLVPIVEPEILVDGTHDIDRCAEVSERVLAACYKALNDHHVLLEGTSLKPNIVTPGSESKKVTPEVIAEYTVRTLQRTVPQAVPGVMFLSGGQSEEEATLNLNAMNKLETKKPWTLSFSYGRALQQSTLKAWQGKEENVAKAQEVFLARAKGNSEATLGKYQGGAG-GADASESLHVKDYKY 357          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF1_PEA (Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 3.870e-82
Identity = 153/198 (77.27%), Postives = 169/198 (85.35%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            +NA GLARYAIICQENGLV  VEPEIL DG HDI KCA VTE VLAACYKALND HVLLEGTLLKPNMVTPGS++PKV+P+VI E+TV AL+RT+PAAVP +VFLSGGQSEE+AT NLNAMN+    KPW+LSFSFGRALQQSTLK W+GK+ENV KAQ  FL RCKANSEATLG Y G +  G  A+ESLHVKDYKY
Sbjct:  161 QNAQGLARYAIICQENGLVLFVEPEILTDGSHDIAKCAAVTETVLAACYKALNDQHVLLEGTLLKPNMVTPGSDSPKVSPEVIGEYTVNALRRTVPAAVPGIVFLSGGQSEEQATLNLNAMNKFDVVKPWTLSFSFGRALQQSTLKTWSGKKENVGKAQDVFLARCKANSEATLGKYGGGSGTGL-ASESLHVKDYKY 357          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALF_ARATH (Fructose-bisphosphate aldolase, cytoplasmic isozyme OS=Arabidopsis thaliana GN=At4g26520 PE=2 SV=2)

HSP 1 Score: 275.404 bits (703), Expect = 2.514e-73
Identity = 139/198 (70.20%), Postives = 159/198 (80.30%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            E+A  LARYAIICQENGLVPIVEPE+L  G HDI KCA VTE VLAA +KALN HHVLLEGTLLKPNMVTPGS++PKV P++IAE+TV AL+RT+P A+P +VFLSG Q EE+AT NLNAMN+L   KPW+L+FSFG ALQQS +KAWAGK ENV KAQA FL RCKAN +ATLG Y G A     A E+L V  Y+Y
Sbjct:  161 EDARVLARYAIICQENGLVPIVEPEVLTGGSHDIKKCAAVTETVLAAVFKALNYHHVLLEGTLLKPNMVTPGSDSPKVAPELIAEYTVTALRRTVPPAIPGIVFLSGIQREEQATLNLNAMNKLDVLKPWTLTFSFGGALQQSAIKAWAGKPENVAKAQAKFLTRCKANKDATLGKYTGWASGDSAAFENLVVIGYRY 358          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALDOB_DANRE (Fructose-bisphosphate aldolase B OS=Danio rerio GN=aldob PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 5.255e-63
Identity = 125/199 (62.81%), Postives = 145/199 (72.86%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPG-SEAPKVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENAN LARYA ICQ+NGLVPIVEPEIL DG HD+ +C   TE+VLAA YKAL+DHHV LEGTLLKPNMVT G S   K TP  +A  TV AL+RT+PAAVP + FLSGGQSEEEA+ NLNAMNQL   +PW LSFS+GRALQ S L AW G+  N + +Q AF+ R K NS A+ G YK   Q G+ + +SL    Y Y
Sbjct:  166 ENANVLARYASICQQNGLVPIVEPEILPDGDHDLKQCQYATEKVLAAVYKALSDHHVYLEGTLLKPNMVTAGHSCTKKYTPLEVAMATVTALRRTVPAAVPGICFLSGGQSEEEASLNLNAMNQLSLHRPWKLSFSYGRALQASALSAWKGQAANKKASQDAFVTRAKINSLASKGEYKPSGQAGQASTQSLFTASYTY 364          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALDOC_PANTR (Fructose-bisphosphate aldolase C OS=Pan troglodytes GN=ALDOC PE=2 SV=3)

HSP 1 Score: 240.35 bits (612), Expect = 8.964e-63
Identity = 120/199 (60.30%), Postives = 148/199 (74.37%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAP-KVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENAN LARYA ICQ+NG+VPIVEPEIL DG HD+ +C  VTE+VLAA YKAL+DHHV LEGTLLKPNMVTPG   P K TP+ IA  TV AL+RT+P AVP V FLSGGQSEEEA+ NLNA+N+    +PW+L+FS+GRALQ S L AW G+ +N + A   F+ R + N  A  G Y+G  + G  AA+SL++ ++ Y
Sbjct:  166 ENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAEAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY 364          
BLAST of CF832440 vs. ExPASy Swiss-Prot
Match: ALDOC_MACFA (Fructose-bisphosphate aldolase C OS=Macaca fascicularis GN=ALDOC PE=2 SV=3)

HSP 1 Score: 239.195 bits (609), Expect = 1.997e-62
Identity = 120/199 (60.30%), Postives = 147/199 (73.87%), Query Frame = 2
Query:    2 ENANGLARYAIICQENGLVPIVEPEILVDGPHDINKCADVTERVLAACYKALNDHHVLLEGTLLKPNMVTPGSEAP-KVTPDVIAEHTVRALQRTMPAAVPAVVFLSGGQSEEEATRNLNAMNQLKTKKPWSLSFSFGRALQQSTLKAWAGKEENVQKAQAAFLVRCKANSEATLGTYKGDAQLGEGAAESLHVKDYKY 595
            ENAN LARYA ICQ+NG+VPIVEPEIL DG HD+ +C  VTE+VLAA YKAL+DHHV LEGTLLKPNMVTPG   P K TP+ IA  TV AL+RT+P AVP V FLSGGQSEEEA+ NLNA+N+    +PW+L+FS+GRALQ S L AW G+ +N   A   F+ R + N  A  G Y+G  + G  AA+SL++ ++ Y
Sbjct:  166 ENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASLNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY 364          
The following BLAST results are available for this feature:
BLAST of CF832440 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 58
Match NameE-valueIdentityDescription
ALF_ORYSJ1.049e-9586.80Fructose-bisphosphate aldolase cytoplasmic isozyme... [more]
ALF_CICAR6.365e-9384.85Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_MAIZE1.200e-9185.28Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF2_PEA5.957e-9183.84Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_SPIOL8.051e-8881.31Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF1_PEA3.870e-8277.27Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALF_ARATH2.514e-7370.20Fructose-bisphosphate aldolase, cytoplasmic isozym... [more]
ALDOB_DANRE5.255e-6362.81Fructose-bisphosphate aldolase B OS=Danio rerio GN... [more]
ALDOC_PANTR8.964e-6360.30Fructose-bisphosphate aldolase C OS=Pan troglodyte... [more]
ALDOC_MACFA1.997e-6260.30Fructose-bisphosphate aldolase C OS=Macaca fascicu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_01N21_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa01N21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832440 ID=CF832440; Name=CF832440; organism=Citrus sinensis; type=EST; length=794bp
TGAGAATGCCAATGGCCTGGCCCGATATGCTATCATCTGCCAGGAGAATG
GCCTTGTTCCCATTGTTGAACCTGAGATCCTTGTTGACGGTCCCCATGAC
ATCAACAAGTGTGCTGATGTGACCGAGCGTGTTCTTGCTGCATGCTACAA
GGCTCTGAATGACCACCATGTCCTTCTTGAGGGTACCCTTTTGAAGCCCA
ACATGGTGACCCCTGGATCTGAAGCTCCCAAAGTTACACCGGACGTGATT
GCTGAACACACTGTCCGGGCCCTGCAACGCACTATGCCTGCAGCAGTTCC
TGCTGTTGTGTTCTTGTCTGGTGGGCAGAGTGAGGAGGAGGCTACCCGCA
ACCTTAATGCCATGAACCAGCTCAAGACGAAGAAGCCATGGTCACTTTCC
TTCTCCTTTGGACGTGCACTTCAGCAGAGCACTCTCAAGGCATGGGCTGG
AAAGGAAGAGAATGTGCAGAAGGCCCAGGCTGCTTTCCTTGTCAGGTGCA
AGGCCAACTCTGAGGCTACCCTCGGTACTTACAAGGGTGATGCCCAGCTT
GGTGAAGGTGCCGCAGAGTCTCTCCATGTCAAGGACTACAAATACTAAGC
ACAGTTGAAGCTTTGGGTGTTGTGCTTTATGAACTTTATGAGAGTAAATG
GCGGTATGAACATTAGGTTGGCCCATTCTCATTCATAGTTTTAGAGCTTT
TTCATATTTAGGTGGAGCAATAAAAAGCTTGGGAACTTCGGTTTGAGTGG
TTTTGATCAAAACTAAACTGAAAACTGTTTATGCTTTTGTTTTT
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